Objective:To evaluate the hepatoprotective effects of the ethanol fraction of Verbascum thapsus L.(EFVT)against CCl4-induced liver injury and elucidate its underlying mechanisms.Methods:The assessment of antioxidant p...Objective:To evaluate the hepatoprotective effects of the ethanol fraction of Verbascum thapsus L.(EFVT)against CCl4-induced liver injury and elucidate its underlying mechanisms.Methods:The assessment of antioxidant properties and cell viability was conducted using the 2,2-diphenyl-1-picrylhydrazyl assay and HepG2 cells,respectively.The in vivo hepatoprotective efficacy of EFVT was evaluated in a rat model of carbon tetrachloride(CCl4)-induced liver injury by determining biochemical parameters,and oxidative stress-and inflammation-related markers.Gas chromatography-mass spectrometry was also employed for the qualitative analysis of its phytochemical composition.Results:GC-MS analysis of EFVT revealed the presence of several bioactive compounds such as 3 methyl mannoside and 9,12-octadecadienoic acid.Oral administration of EFVT significantly mitigated CCl4-induced liver injury,as evidenced by reduced levels of total bilirubin,alkaline phosphatase,alanine aminotransferase,aspartate aminotransferase,and malondialdehyde,boosted activities of catalase and superoxide dismutase,as well as enhanced glutathione levels.Histopathological examinations indicated EFVT restored abnormal liver architecture and reduced inflammation.Additionally,EFVT substantially downregulated the mRNA levels of IL-6,IL-1β,TNF-α,and NF-κB,and upregulated IL-10 expression.Conclusions:These findings underscore the therapeutic potential of EFVT in ameliorating liver damage associated with oxidative stress,providing scientific validation for its traditional utilization in ethnomedicine.展开更多
CO_(2)fumigation has been extensively used in greenhouses cultivation to enhance crop yield.The effects under the precise level of elevated CO_(2)(e[CO_(2)])on crop morphology,yield,and fruit quality remain largely el...CO_(2)fumigation has been extensively used in greenhouses cultivation to enhance crop yield.The effects under the precise level of elevated CO_(2)(e[CO_(2)])on crop morphology,yield,and fruit quality remain largely elusive yet.To explore the response of plant growth to the continuous RCPs(Representative Concentration Pathways)projected CO_(2)concentration[CO_(2)],tomato(Hezuo 908)plants were grown under ambient CO_(2)(a[CO_(2)],462μmol/mol)and e[CO_(2)](550,700,850 and 1000μmol/mol):named as EC550,EC_(700),EC_(850),and EC_(1000),respectively,under uniform environmental condition for two planting seasons.Collective growth of tomato plants(plant height,stem diameter,and leaf area index)was significantly enhanced under EC_(700)and showed a slightly negative response under EC_(850).The optimum yield was stimulated under EC_(700)by 74.05%and 55.91%,while maximum total dry weight(DW_(t))was enhanced under EC_(1000)by 58.23%and 39.78%during autumn-winter and spring-summer planting seasons,respectively,as compared to a[CO_(2)].The greatest yield and least DWt stimulated under EC_(700)for both seasons indicated that EC_(700)improved the ability of the tomato plants to translocate carbohydrates to fruits.Optimum water use efficiency related to yield(WUE_(y))was enhanced by 55.91-210.87%under EC_(700)compared to a[CO_(2)].The titratable acid(TA)was improved by 19.94%(EC_(700)),29.17%(EC_(850)),and 97.92%(EC_(1000)),and the lycopene(Lp)was increased by 2.22%(EC_(700))and reduced by 2.28%(EC_(1000)).Thus,the overall optimum impact on tomato growth was explored under EC_(700).Super e[CO_(2)]did not positively influence the tomato growth process and yield under adequate water and fertilizer conditions.The present study results are beneficial for greenhouse crop production and might be used as a reference to validate the climate change influence modeling.展开更多
文摘Objective:To evaluate the hepatoprotective effects of the ethanol fraction of Verbascum thapsus L.(EFVT)against CCl4-induced liver injury and elucidate its underlying mechanisms.Methods:The assessment of antioxidant properties and cell viability was conducted using the 2,2-diphenyl-1-picrylhydrazyl assay and HepG2 cells,respectively.The in vivo hepatoprotective efficacy of EFVT was evaluated in a rat model of carbon tetrachloride(CCl4)-induced liver injury by determining biochemical parameters,and oxidative stress-and inflammation-related markers.Gas chromatography-mass spectrometry was also employed for the qualitative analysis of its phytochemical composition.Results:GC-MS analysis of EFVT revealed the presence of several bioactive compounds such as 3 methyl mannoside and 9,12-octadecadienoic acid.Oral administration of EFVT significantly mitigated CCl4-induced liver injury,as evidenced by reduced levels of total bilirubin,alkaline phosphatase,alanine aminotransferase,aspartate aminotransferase,and malondialdehyde,boosted activities of catalase and superoxide dismutase,as well as enhanced glutathione levels.Histopathological examinations indicated EFVT restored abnormal liver architecture and reduced inflammation.Additionally,EFVT substantially downregulated the mRNA levels of IL-6,IL-1β,TNF-α,and NF-κB,and upregulated IL-10 expression.Conclusions:These findings underscore the therapeutic potential of EFVT in ameliorating liver damage associated with oxidative stress,providing scientific validation for its traditional utilization in ethnomedicine.
基金supported by the Natural Science Foundation of China(Grant No.51509107,51609103,41860863)Belt and Road Special Foundation of the State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering(Grant No.2020nkzd01)Postdoctoral Research of Jiangsu Province(Grant No.Bs510001),Open Fund of High-tech Key Laboratory of Agricultural Equipment and Intelligentization of Jiangsu Province and Faculty of Agricultural Equipment of Jiangsu University for financial support(Grant No.JNZ201917).
文摘CO_(2)fumigation has been extensively used in greenhouses cultivation to enhance crop yield.The effects under the precise level of elevated CO_(2)(e[CO_(2)])on crop morphology,yield,and fruit quality remain largely elusive yet.To explore the response of plant growth to the continuous RCPs(Representative Concentration Pathways)projected CO_(2)concentration[CO_(2)],tomato(Hezuo 908)plants were grown under ambient CO_(2)(a[CO_(2)],462μmol/mol)and e[CO_(2)](550,700,850 and 1000μmol/mol):named as EC550,EC_(700),EC_(850),and EC_(1000),respectively,under uniform environmental condition for two planting seasons.Collective growth of tomato plants(plant height,stem diameter,and leaf area index)was significantly enhanced under EC_(700)and showed a slightly negative response under EC_(850).The optimum yield was stimulated under EC_(700)by 74.05%and 55.91%,while maximum total dry weight(DW_(t))was enhanced under EC_(1000)by 58.23%and 39.78%during autumn-winter and spring-summer planting seasons,respectively,as compared to a[CO_(2)].The greatest yield and least DWt stimulated under EC_(700)for both seasons indicated that EC_(700)improved the ability of the tomato plants to translocate carbohydrates to fruits.Optimum water use efficiency related to yield(WUE_(y))was enhanced by 55.91-210.87%under EC_(700)compared to a[CO_(2)].The titratable acid(TA)was improved by 19.94%(EC_(700)),29.17%(EC_(850)),and 97.92%(EC_(1000)),and the lycopene(Lp)was increased by 2.22%(EC_(700))and reduced by 2.28%(EC_(1000)).Thus,the overall optimum impact on tomato growth was explored under EC_(700).Super e[CO_(2)]did not positively influence the tomato growth process and yield under adequate water and fertilizer conditions.The present study results are beneficial for greenhouse crop production and might be used as a reference to validate the climate change influence modeling.
