Exosomes are extracellular vesicles with sizes from 30 to 150 nm in diameter and modulate the transport of multiple intracellular biological molecules including proteins,nucleic acids,lipids,and metabolites.They regul...Exosomes are extracellular vesicles with sizes from 30 to 150 nm in diameter and modulate the transport of multiple intracellular biological molecules including proteins,nucleic acids,lipids,and metabolites.They regulate a large number of cells and are involved in different pathological and physiological activities including carcinogenesis,viral infection,cell-cell communication and immune responses as well.Stem cell-derived exosomes carry many benefits over simple stem cells in the form of easy access,freedom from tumourigenic capabilities,non-infusion toxicity,effortless preservation,and immunogenicity.Exosomes have almost the same properties and perform functions effectively in the same way as their parental cells do like adult stem cells and embryonic stem cells.Due to their pluripotent or multipotent abilities,stem cells(SCs)transform into several types of cells.In addition to other secretions,SC also give exosomes,which in turn shows therapeutic significance for many disorders,including cancer,diabetes mellitus,skin allergies and regenerative medicine.Exosomes originating from mesenchymal stem cells(MSCs)have miRNAs,lipids,and proteins that trigger diabetes and cancer situations in humans.Exosomes from SCs(sc-exos)are preferred to SC as there are fewer side effects and other challenges,including effectiveness,drug delivery,lower immunogenicity and tumourigenicity.In the current review,we summarize the data from the last 5 years'articles about exosomes and stem cell-derived microvesicles for the therapeutic potential of various diseases such as cancer,Alzheimer's disease,diabetes,and Parkinson's disease with clinical challenges and future aspects.展开更多
Tumor protein p53 (TP53) mediates DNA repair and cell proliferation in growing cells. The TP53 gene is a tumor suppressor that regulates the expression of target genes in response to multiple cellular stress factors. ...Tumor protein p53 (TP53) mediates DNA repair and cell proliferation in growing cells. The TP53 gene is a tumor suppressor that regulates the expression of target genes in response to multiple cellular stress factors. Key target genes are involved in crucial cellular events such as DNA repair, cell cycle regulation, apoptosis, metabolism, and senescence. TP53 genetic variants and the activity of the wild-type p53 protein (WT-p53) have been linked to a wide range of tumorigenesis. Various genetic and epigenetic alterations, including germline and somatic mutations, loss of heterozygosity, and DNA methylation, can alter TP53 activity, potentially resulting in cancer initiation and progression. This study was designed to screen three reported mutations in the DNA-binding domain of the p53 protein in breast cancer, to evaluate the relative susceptibility and risk associated with breast cancer in the local population. Genomic DNA was isolated from 30 breast tumor tissues along with controls. Tetra and Tri ARMS PCR were performed to detect mutations in the TP53 coding region. For SNPs c.637C>T and c.733C>T, all analyzed cases were homozygous for the wild-type allele ‘C,’ while for SNP c.745A>G, all cases were homozygous for the wild-type allele ‘A.’ These results indicate no relevance of these three SNPs to cancer progression in our study cohort. Additionally, the findings from whole exon sequencing will help to predict more precise outcomes and assess the importance of TP53 gene mutations in breast cancer patients.展开更多
TP53 is a tumor suppressor gene that is mutated in most cancer types and has been extensively studied in cancer research.p53 plays a critical role in regulating the expression of target genes and is involved in key pr...TP53 is a tumor suppressor gene that is mutated in most cancer types and has been extensively studied in cancer research.p53 plays a critical role in regulating the expression of target genes and is involved in key processes such as apoptosis,cell cycle regulation,and genomic stability,earning it the title“guardian of the genome.”Numerous studies have demonstrated p53’s influence on and regulation of autophagy,ferroptosis,the tumor microenvironment,and cell metabolism,all of which contribute to tumor suppression.Alterations in p53,specifically mutant p53(mutp53),not only impair its tumor-suppressing functions but also enhance oncogenic characteristics.Recent data indicate that mutp53 is strongly associated with poor prognosis and advanced cancers,making it an ideal target for the development of novel cancer therapies.This review summarizes the post-translational modifications of p53,the mechanisms of mutp53 accumulation,and its gain-of-function,based on previous findings.Additionally,this review discusses its impact on metabolic homeostasis,ferroptosis,genomic instability,the tumor microenvironment,and cancer stem cells,and highlights recent advancements in mutp53 research.展开更多
Nanotechnology has far-reaching implications and applications in multiple fields.The biomedical and health sectors can use nanotechnology concepts for medication delivery and treatment.Under controlled conditions,it c...Nanotechnology has far-reaching implications and applications in multiple fields.The biomedical and health sectors can use nanotechnology concepts for medication delivery and treatment.Under controlled conditions,it can target and initiate administering drugs and several other therapeutic agents.Since cancer is the largest cause of death worldwide,prompt diagnosis and effective anticancer treatments are crucial.In this particular context,nanotechnology reduces side effects and directs drug delivery to specifically target cancer cells,providing unique benefits for cancer therapy.In the present thorough review,the most noteworthy new findings for 2010–2023 were compiled,which address the development and use of nanosystems for cancer treatment.Nanoparticles allow precise and controlled release of therapeutic substances at specific action locations,enabling targeted medication delivery.Size,shape,surface,charge,and loading methods impact its efficiency.Researchers have made advancements in encapsulating drugs into nanoliposomes and nanoemulsions,including paclitaxel and fisetin,and are currently testing their suitability in ongoing clinical trials.The purpose of this review is to serve as a continuous path toward recognizing the extraordinary potential of various nanoparticles in cancer therapies.展开更多
Cancer stem cells(CSCs),or tumor-initiating cells(TICs),are cancerous cell subpopulations that remain while tumor cells propagate as a unique subset and exhibit multiple applications in several diseases.They are respo...Cancer stem cells(CSCs),or tumor-initiating cells(TICs),are cancerous cell subpopulations that remain while tumor cells propagate as a unique subset and exhibit multiple applications in several diseases.They are responsible for cancer cell initiation,development,metastasis,proliferation,and recurrence due to their self-renewal and differentiation abilities in many kinds of cells.Artificial intelligence(AI)has gained significant attention because of its vast applications in various fields including agriculture,healthcare,transportation,and robotics,particularly in detecting human diseases such as cancer.The division and metastasis of cancerous cells are not easy to identify at early stages due to their uncontrolled situations.It has provided some real-time pictures of cancer progression and relapse.The purpose of this review paper is to explore new investigations into the role of AI in cancer stem cell progression and metastasis and in regenerative medicines.It describes the association of machine learning and AI with CSCs along with its numerous applications from cancer diagnosis to therapy.This review has also provided key challenges and future directions of AI in cancer stem cell research diagnosis and therapeutic approach.展开更多
MicroRNAs(miRNAs)are small non-coding RNAs,19-25 nucleotides in length,that regulate gene expression.miRNAs are involved in various cellular,biological,and pathological activities,including the development and progres...MicroRNAs(miRNAs)are small non-coding RNAs,19-25 nucleotides in length,that regulate gene expression.miRNAs are involved in various cellular,biological,and pathological activities,including the development and progression of malignant tumors.Numerous studies have highlighted the significant roles of miRNA deregulation in various cancer types,involving critical pathways such as gene deletions and amplifications,abnormal epigenetic changes,and dysfunctional miRNA regulatory systems.miRNAs can function both as oncogenes and tumor suppressor genes in different contexts.Several cancer hallmarks are associated with deregulated miRNAs,including resistance to cell death,metastasis,sustained proliferative signaling,promotion of angiogenesis,and the suppression of growth factors.Recent research has demonstrated that miRNAs contribute to drug resistance in tumor cells by targeting genes linked to drug resistance or by influencing genes that regulate the cell cycle,apoptosis,and proliferation.A single miRNA often has tissue-specific regulatory functions and can affect multiple genes.Various miRNA types have been identified as potential biomarkers for tumor diagnosis and critical targets for novel therapeutic approaches.This review aims to explore the role of miRNAs in cancer progression,metastasis,and potential therapeutic interventions.展开更多
Cancer stem cells(CSCs),first identified in blood cancers,are increasingly recognized as significant biomarkers and targets in tumor therapy due to their metastatic potential and role in cancer recurrence.Recent resea...Cancer stem cells(CSCs),first identified in blood cancers,are increasingly recognized as significant biomarkers and targets in tumor therapy due to their metastatic potential and role in cancer recurrence.Recent research has demonstrated the dedication of scientists in targeting CSCs to explore novel therapeutic strategies.Many types of cancer exhibit metastasis,heterogeneity,and resistance to treatment,all of which are influenced by CSCs.These cells utilize various transcription factors and signaling pathways to carry out these functions.By identifying and understanding these pathways,new therapeutic breakthroughs can be achieved.Thus,targeting cancer stem cells holds great potential and importance in cancer treatment.Moreover,CSCs offer promising avenues for treating otherwise incurable diseases.However,targeting CSCs presents challenges such as immunological rejection and disease recurrence.Advancing research into CSCs may reveal new insights in the fight against cancer and ultimately improve human health.This review explores the roles of CSCs in cancer development and treatment,aiming to uncover new therapeutic approaches.展开更多
The present study describes the frequency of Foot and Mouth Disease （FMD） virus serotypes （O, A and Asia-l） in major regions （all provinces） of Pakistan using Indirect Sandwich ELISA. Also, spatial distribution ...The present study describes the frequency of Foot and Mouth Disease （FMD） virus serotypes （O, A and Asia-l） in major regions （all provinces） of Pakistan using Indirect Sandwich ELISA. Also, spatial distribution of various FMD serotypes and their comparison is discussed. A total of 590 samples （Epithelial tissue） have been analyzed during a period of five years （2005-2009）. Out of 590 samples, 180 were found positive, giving an overall confirmation of FMDV about 33.2 %. Of the prevalent serotypes, FMDV ＇O＇ serotype caused most outbreaks （20.7 %）, followed by serotype A （6.6 %） and serotype Asia-1 （4.6 %） while there was no positive case of type ＇C＇. The study clearly showed that the disease was more frequent in the agro-climatic zones than in hilly areas. Based on the data of 590 samples （〉50 outbreaks）, the overall prevalence of FMDV in cattle and buffaloes in Pakistan was 33.2 %, while in cattle alone, it was 37.1%, higher than in buffalo （28.7 %）. There were eight cases of mixed serotypes infection, indicating the presence of endemic state of disease. Another significant feature was the change over time. In phase-I （2005-2007）, there was an overall prevalence of 29.4 %, while the occurrence of the serotype O, A and Asia-1 was 20.4 %, 2.9 % and 4.7 %, respectively. During phase-II （2008-2009）, the overall prevalence was 59.21%, while those of serotype O, A and Asia-1 were 22.4 %, 31.6 % and 4.0 %, respectively. This clearly indicated a shift from serotype O to A, which may help to explain the occurrence of more severe outbreaks, despite vaccination.展开更多
The sensitivity, specificity and reproducibility of the serological tests for detection of avian in-fluenza viruses were carried-out by using Ham- agglutination inhibition (HI), Agar gel precipita-tion test (AGPT), an...The sensitivity, specificity and reproducibility of the serological tests for detection of avian in-fluenza viruses were carried-out by using Ham- agglutination inhibition (HI), Agar gel precipita-tion test (AGPT), and Enzyme linked immu-nosorbent assay (ELISA) and Serum neutraliza-tion test. The geometric mean titre (GMT) of hae- magglutination inhibition antibodies recor- ded as log2 indicated that the post vaccination titres in the field were on higher side i.e., 7.9 for H7 and 5.9 for H9. The correlation between HI titre and AGPT affirmed that for the AGPT test need high antibody titre for positive reaction. The pooled sera were also used to correlate the se-rum neutralization test and enzyme linked im- muno-sorbent assay. The serial two fold dilute- ons were tested for the serum neutralization ac- tivity and concluded that the HI titre log2 4 pro-vided 100% protection, than 52% and 45% pro-tection in 1:2 and 1:4 dilution was recorded, respectively. Similarly, the ELISA test showed positive results up to 1:16 HI titre, i.e. log2 4 and confirmed the linear relation between these two serological tests. In HI test, the concentration of antigen can influence the result. It also needs careful preparation of concentration of eryth-rocyte suspension. Agar Gel immuno-diffusion is basically a qualitative test as it can not de-termine the quantity of antigen or antibody with the help of this test. It lacks the level of sensi-tivity as offered by other test. If serum neutrali-zation test is performed on a pooled serum sam- ples, then it could lead to a false conclusion on antibodies status. ELISA is most sensitive, spe-cific and accurate as compare to all other sero-logical tests.展开更多
Background Hepatitis B virus (HBV) is the major etiological agent causing acute and chronic liver disease worldwide with significant morbidity and mortality. The high genetic variability of HBV is reflected by eight g...Background Hepatitis B virus (HBV) is the major etiological agent causing acute and chronic liver disease worldwide with significant morbidity and mortality. The high genetic variability of HBV is reflected by eight genotypes (A to H), each with a particular geographical prevalence. The global pattern of HBV genotypes is associated with the distribution of human population among the different continents and reflects the patterns of human migrations. Objectives This study was conducted with following objectives: 1) To study the prevalence of HBV genotype in Pakistani population;2) To assess that the RFLP system is simple, rapid and standardized way of identifying HBV genotype. Study Design & Method In cross-sectional study design a total of 255 HBV ELISA positive samples were studied in order to identify the most prevalent genotypes in Pakistan. These HBV related patients visited various hospitals in Pakistan at Faisalabad, Lahore and Islamabad. Among these samples, 214 were PCR positive and rest 41 were PCR negative for HBV. S-gene of HBV PCR positive samples was amplified by regular (first round) and nested PCR (second round). Second-round PCR products were digested by Restriction Fragment Length Polymorphism (RFLP). This was carried out using five restriction enzymes (HphI, NciI, AlwI, EarI and NlaIV) that identified the genotype-specific sequences. Results & Conclusion Among (214) PCR positive samples only genotype C and D were identified in local population with 21 cases (9.81%) of genotype C and 195 (91.1%) of genotype D. Hence, the algorithm adopted in this study can be used to identify various HBV genotypes.展开更多
Model based implementation of a novel nonlinear adaptive filter for extraction of time varying sinusoids using Xilinx system generator has been presented in this work. The practicality of this filter model along with ...Model based implementation of a novel nonlinear adaptive filter for extraction of time varying sinusoids using Xilinx system generator has been presented in this work. The practicality of this filter model along with its performance makes it one of the foremost candidates to be applied on nonlinear systems for the purpose of estimation and extraction using reconfigurable hardware like FPGA. A design implementation and verification approach has been discussed for more efficient implementation. Timing and power analysis has been performed and the architecture has been optimized for speed and power to perform at higher frequency when integrated on a Xilinx FPGA. The proposed hardware oriented architecture has been successfully implemented and simulated. The simulation results to track a noisy input have also been shown to demonstrate the exceptional performance of the hardware based architecture developed.展开更多
文摘Exosomes are extracellular vesicles with sizes from 30 to 150 nm in diameter and modulate the transport of multiple intracellular biological molecules including proteins,nucleic acids,lipids,and metabolites.They regulate a large number of cells and are involved in different pathological and physiological activities including carcinogenesis,viral infection,cell-cell communication and immune responses as well.Stem cell-derived exosomes carry many benefits over simple stem cells in the form of easy access,freedom from tumourigenic capabilities,non-infusion toxicity,effortless preservation,and immunogenicity.Exosomes have almost the same properties and perform functions effectively in the same way as their parental cells do like adult stem cells and embryonic stem cells.Due to their pluripotent or multipotent abilities,stem cells(SCs)transform into several types of cells.In addition to other secretions,SC also give exosomes,which in turn shows therapeutic significance for many disorders,including cancer,diabetes mellitus,skin allergies and regenerative medicine.Exosomes originating from mesenchymal stem cells(MSCs)have miRNAs,lipids,and proteins that trigger diabetes and cancer situations in humans.Exosomes from SCs(sc-exos)are preferred to SC as there are fewer side effects and other challenges,including effectiveness,drug delivery,lower immunogenicity and tumourigenicity.In the current review,we summarize the data from the last 5 years'articles about exosomes and stem cell-derived microvesicles for the therapeutic potential of various diseases such as cancer,Alzheimer's disease,diabetes,and Parkinson's disease with clinical challenges and future aspects.
文摘Tumor protein p53 (TP53) mediates DNA repair and cell proliferation in growing cells. The TP53 gene is a tumor suppressor that regulates the expression of target genes in response to multiple cellular stress factors. Key target genes are involved in crucial cellular events such as DNA repair, cell cycle regulation, apoptosis, metabolism, and senescence. TP53 genetic variants and the activity of the wild-type p53 protein (WT-p53) have been linked to a wide range of tumorigenesis. Various genetic and epigenetic alterations, including germline and somatic mutations, loss of heterozygosity, and DNA methylation, can alter TP53 activity, potentially resulting in cancer initiation and progression. This study was designed to screen three reported mutations in the DNA-binding domain of the p53 protein in breast cancer, to evaluate the relative susceptibility and risk associated with breast cancer in the local population. Genomic DNA was isolated from 30 breast tumor tissues along with controls. Tetra and Tri ARMS PCR were performed to detect mutations in the TP53 coding region. For SNPs c.637C>T and c.733C>T, all analyzed cases were homozygous for the wild-type allele ‘C,’ while for SNP c.745A>G, all cases were homozygous for the wild-type allele ‘A.’ These results indicate no relevance of these three SNPs to cancer progression in our study cohort. Additionally, the findings from whole exon sequencing will help to predict more precise outcomes and assess the importance of TP53 gene mutations in breast cancer patients.
文摘TP53 is a tumor suppressor gene that is mutated in most cancer types and has been extensively studied in cancer research.p53 plays a critical role in regulating the expression of target genes and is involved in key processes such as apoptosis,cell cycle regulation,and genomic stability,earning it the title“guardian of the genome.”Numerous studies have demonstrated p53’s influence on and regulation of autophagy,ferroptosis,the tumor microenvironment,and cell metabolism,all of which contribute to tumor suppression.Alterations in p53,specifically mutant p53(mutp53),not only impair its tumor-suppressing functions but also enhance oncogenic characteristics.Recent data indicate that mutp53 is strongly associated with poor prognosis and advanced cancers,making it an ideal target for the development of novel cancer therapies.This review summarizes the post-translational modifications of p53,the mechanisms of mutp53 accumulation,and its gain-of-function,based on previous findings.Additionally,this review discusses its impact on metabolic homeostasis,ferroptosis,genomic instability,the tumor microenvironment,and cancer stem cells,and highlights recent advancements in mutp53 research.
文摘Nanotechnology has far-reaching implications and applications in multiple fields.The biomedical and health sectors can use nanotechnology concepts for medication delivery and treatment.Under controlled conditions,it can target and initiate administering drugs and several other therapeutic agents.Since cancer is the largest cause of death worldwide,prompt diagnosis and effective anticancer treatments are crucial.In this particular context,nanotechnology reduces side effects and directs drug delivery to specifically target cancer cells,providing unique benefits for cancer therapy.In the present thorough review,the most noteworthy new findings for 2010–2023 were compiled,which address the development and use of nanosystems for cancer treatment.Nanoparticles allow precise and controlled release of therapeutic substances at specific action locations,enabling targeted medication delivery.Size,shape,surface,charge,and loading methods impact its efficiency.Researchers have made advancements in encapsulating drugs into nanoliposomes and nanoemulsions,including paclitaxel and fisetin,and are currently testing their suitability in ongoing clinical trials.The purpose of this review is to serve as a continuous path toward recognizing the extraordinary potential of various nanoparticles in cancer therapies.
文摘Cancer stem cells(CSCs),or tumor-initiating cells(TICs),are cancerous cell subpopulations that remain while tumor cells propagate as a unique subset and exhibit multiple applications in several diseases.They are responsible for cancer cell initiation,development,metastasis,proliferation,and recurrence due to their self-renewal and differentiation abilities in many kinds of cells.Artificial intelligence(AI)has gained significant attention because of its vast applications in various fields including agriculture,healthcare,transportation,and robotics,particularly in detecting human diseases such as cancer.The division and metastasis of cancerous cells are not easy to identify at early stages due to their uncontrolled situations.It has provided some real-time pictures of cancer progression and relapse.The purpose of this review paper is to explore new investigations into the role of AI in cancer stem cell progression and metastasis and in regenerative medicines.It describes the association of machine learning and AI with CSCs along with its numerous applications from cancer diagnosis to therapy.This review has also provided key challenges and future directions of AI in cancer stem cell research diagnosis and therapeutic approach.
文摘MicroRNAs(miRNAs)are small non-coding RNAs,19-25 nucleotides in length,that regulate gene expression.miRNAs are involved in various cellular,biological,and pathological activities,including the development and progression of malignant tumors.Numerous studies have highlighted the significant roles of miRNA deregulation in various cancer types,involving critical pathways such as gene deletions and amplifications,abnormal epigenetic changes,and dysfunctional miRNA regulatory systems.miRNAs can function both as oncogenes and tumor suppressor genes in different contexts.Several cancer hallmarks are associated with deregulated miRNAs,including resistance to cell death,metastasis,sustained proliferative signaling,promotion of angiogenesis,and the suppression of growth factors.Recent research has demonstrated that miRNAs contribute to drug resistance in tumor cells by targeting genes linked to drug resistance or by influencing genes that regulate the cell cycle,apoptosis,and proliferation.A single miRNA often has tissue-specific regulatory functions and can affect multiple genes.Various miRNA types have been identified as potential biomarkers for tumor diagnosis and critical targets for novel therapeutic approaches.This review aims to explore the role of miRNAs in cancer progression,metastasis,and potential therapeutic interventions.
文摘Cancer stem cells(CSCs),first identified in blood cancers,are increasingly recognized as significant biomarkers and targets in tumor therapy due to their metastatic potential and role in cancer recurrence.Recent research has demonstrated the dedication of scientists in targeting CSCs to explore novel therapeutic strategies.Many types of cancer exhibit metastasis,heterogeneity,and resistance to treatment,all of which are influenced by CSCs.These cells utilize various transcription factors and signaling pathways to carry out these functions.By identifying and understanding these pathways,new therapeutic breakthroughs can be achieved.Thus,targeting cancer stem cells holds great potential and importance in cancer treatment.Moreover,CSCs offer promising avenues for treating otherwise incurable diseases.However,targeting CSCs presents challenges such as immunological rejection and disease recurrence.Advancing research into CSCs may reveal new insights in the fight against cancer and ultimately improve human health.This review explores the roles of CSCs in cancer development and treatment,aiming to uncover new therapeutic approaches.
基金Food & Agriculture Organization FMD Project"Progressive Control of Foot and Mouth Disease in Pakistan(GCP/PAK/123/USA)the FAO (GTFS/INT/907/ITA) and EU(SLSP) funded projects
文摘The present study describes the frequency of Foot and Mouth Disease （FMD） virus serotypes （O, A and Asia-l） in major regions （all provinces） of Pakistan using Indirect Sandwich ELISA. Also, spatial distribution of various FMD serotypes and their comparison is discussed. A total of 590 samples （Epithelial tissue） have been analyzed during a period of five years （2005-2009）. Out of 590 samples, 180 were found positive, giving an overall confirmation of FMDV about 33.2 %. Of the prevalent serotypes, FMDV ＇O＇ serotype caused most outbreaks （20.7 %）, followed by serotype A （6.6 %） and serotype Asia-1 （4.6 %） while there was no positive case of type ＇C＇. The study clearly showed that the disease was more frequent in the agro-climatic zones than in hilly areas. Based on the data of 590 samples （〉50 outbreaks）, the overall prevalence of FMDV in cattle and buffaloes in Pakistan was 33.2 %, while in cattle alone, it was 37.1%, higher than in buffalo （28.7 %）. There were eight cases of mixed serotypes infection, indicating the presence of endemic state of disease. Another significant feature was the change over time. In phase-I （2005-2007）, there was an overall prevalence of 29.4 %, while the occurrence of the serotype O, A and Asia-1 was 20.4 %, 2.9 % and 4.7 %, respectively. During phase-II （2008-2009）, the overall prevalence was 59.21%, while those of serotype O, A and Asia-1 were 22.4 %, 31.6 % and 4.0 %, respectively. This clearly indicated a shift from serotype O to A, which may help to explain the occurrence of more severe outbreaks, despite vaccination.
文摘The sensitivity, specificity and reproducibility of the serological tests for detection of avian in-fluenza viruses were carried-out by using Ham- agglutination inhibition (HI), Agar gel precipita-tion test (AGPT), and Enzyme linked immu-nosorbent assay (ELISA) and Serum neutraliza-tion test. The geometric mean titre (GMT) of hae- magglutination inhibition antibodies recor- ded as log2 indicated that the post vaccination titres in the field were on higher side i.e., 7.9 for H7 and 5.9 for H9. The correlation between HI titre and AGPT affirmed that for the AGPT test need high antibody titre for positive reaction. The pooled sera were also used to correlate the se-rum neutralization test and enzyme linked im- muno-sorbent assay. The serial two fold dilute- ons were tested for the serum neutralization ac- tivity and concluded that the HI titre log2 4 pro-vided 100% protection, than 52% and 45% pro-tection in 1:2 and 1:4 dilution was recorded, respectively. Similarly, the ELISA test showed positive results up to 1:16 HI titre, i.e. log2 4 and confirmed the linear relation between these two serological tests. In HI test, the concentration of antigen can influence the result. It also needs careful preparation of concentration of eryth-rocyte suspension. Agar Gel immuno-diffusion is basically a qualitative test as it can not de-termine the quantity of antigen or antibody with the help of this test. It lacks the level of sensi-tivity as offered by other test. If serum neutrali-zation test is performed on a pooled serum sam- ples, then it could lead to a false conclusion on antibodies status. ELISA is most sensitive, spe-cific and accurate as compare to all other sero-logical tests.
文摘Background Hepatitis B virus (HBV) is the major etiological agent causing acute and chronic liver disease worldwide with significant morbidity and mortality. The high genetic variability of HBV is reflected by eight genotypes (A to H), each with a particular geographical prevalence. The global pattern of HBV genotypes is associated with the distribution of human population among the different continents and reflects the patterns of human migrations. Objectives This study was conducted with following objectives: 1) To study the prevalence of HBV genotype in Pakistani population;2) To assess that the RFLP system is simple, rapid and standardized way of identifying HBV genotype. Study Design & Method In cross-sectional study design a total of 255 HBV ELISA positive samples were studied in order to identify the most prevalent genotypes in Pakistan. These HBV related patients visited various hospitals in Pakistan at Faisalabad, Lahore and Islamabad. Among these samples, 214 were PCR positive and rest 41 were PCR negative for HBV. S-gene of HBV PCR positive samples was amplified by regular (first round) and nested PCR (second round). Second-round PCR products were digested by Restriction Fragment Length Polymorphism (RFLP). This was carried out using five restriction enzymes (HphI, NciI, AlwI, EarI and NlaIV) that identified the genotype-specific sequences. Results & Conclusion Among (214) PCR positive samples only genotype C and D were identified in local population with 21 cases (9.81%) of genotype C and 195 (91.1%) of genotype D. Hence, the algorithm adopted in this study can be used to identify various HBV genotypes.
文摘Model based implementation of a novel nonlinear adaptive filter for extraction of time varying sinusoids using Xilinx system generator has been presented in this work. The practicality of this filter model along with its performance makes it one of the foremost candidates to be applied on nonlinear systems for the purpose of estimation and extraction using reconfigurable hardware like FPGA. A design implementation and verification approach has been discussed for more efficient implementation. Timing and power analysis has been performed and the architecture has been optimized for speed and power to perform at higher frequency when integrated on a Xilinx FPGA. The proposed hardware oriented architecture has been successfully implemented and simulated. The simulation results to track a noisy input have also been shown to demonstrate the exceptional performance of the hardware based architecture developed.
