Neural image compression(NIC)has shown remarkable rate-distortion(R-D)efficiency.However,the considerable computational and spatial complexity of most NIC methods presents deployment challenges on resource-constrained...Neural image compression(NIC)has shown remarkable rate-distortion(R-D)efficiency.However,the considerable computational and spatial complexity of most NIC methods presents deployment challenges on resource-constrained devices.We introduce a lightweight neural image compression framework designed to efficiently process both local and global information.In this framework,the convolutional branch extracts local information,whereas the frequency domain branch extracts global information.To capture global information without the high computational costs of dense pixel operations,such as attention mechanisms,Fourier transform is employed.This approach allows for the manipulation of global information in the frequency domain.Additionally,we employ feature shift operations as a strategy to acquire large receptive fields without any computational cost,thus circumventing the need for large kernel convolution.Our framework achieves a superior balance between ratedistortion performance and complexity.On varying resolution sets,our method not only achieves rate-distortion(R-D)performance on par with versatile video coding(VVC)intra and other state-of-the-art(SOTA)NIC methods but also exhibits the lowest computational requirements,with approximately 200 KMACs/pixel.The code will be available at https://github.com/baoyu2020/SFNIC.展开更多
As the well drilling depth has broken through the 10,000 m in China,accurate measurements of downhole engineering parameters,such as annulus temperature and pressure for the whole wellbore,are significant in controlli...As the well drilling depth has broken through the 10,000 m in China,accurate measurements of downhole engineering parameters,such as annulus temperature and pressure for the whole wellbore,are significant in controlling potential downhole complexities.In this present work,a new micro-measurer is developed by integrating measurements of downhole temperature,pressure,magnetic field strength,and its own dynamic signals.The micro-measurer can flow with drilling fluid from the drillstring to the bottomhole and then float up back to the ground via the wellbore annulus.Compared with other downhole measurement tools that are fixedly connected to the drill string,its“measure-and-move-on”approach reduces the residence time in the high-temperature and high-pressure zone at the bottomhole;moreover,both the pressure and temperature at different well depth can be measured,thereby the temperature and pressure profiles of the whole wellbore can be constructed.In addition,the bluetooth low energy(BLE)technique is applied to offer the micro-measurer with the capability of wireless information transmission;while hydrodynamic optimization of the micro-measurer is carried out to design the structure of the micro-measurer,which can promote its recovery rate from downhole.In addition,an intelligent joint for releasing micro-measurers from the wellbore annulus is also proposed,aiming to overcome the limitation imposed by the nozzle on the size of the micro-measurer.Both the indoor experiments and the field tests have verified the feasibility of the newly designed micro-measurer,which is a key step for establishing a complete downhole internet of things(IoT)system to serve the intelligent drilling in the future.展开更多
Objective The clinical features,disease course and visual outcomes of toxoplasmosis are less commonly reported in China than in other countries.To reduce misdiagnosis and improve visual function,the clinical character...Objective The clinical features,disease course and visual outcomes of toxoplasmosis are less commonly reported in China than in other countries.To reduce misdiagnosis and improve visual function,the clinical characteristics,management and visual outcomes of 13 cases of ocular toxoplasmosis(OT)were described.Methods This retrospective study included 14 eyes of 13 patients who were diagnosed with OT in Hubei,China.The clinical characteristics,course of treatment and outcomes are presented.There were 7 males and 6 females.Results The main form of OT was retinochoroiditis with vitritis or anterior uveitis.Next-generation sequencing was applied to 3 eyes,and positive results were found in those eyes.Thirteen patients were positive for Toxoplasma gondii IgG antibodies,and 3 of them were also positive for IgM T.gondii antibodies.One patient with acquired immune deficiency syndrome was diagnosed with coinfection with OT and cytomegalovirus,as evidenced by an aqueous humor etiological test.Three patients were misdiagnosed with noninfectious uveitis.Recurrence occurred in 3 eyes during the follow-up periods.One patient who received vitreous implantation of Ozurdex therapy at another hospital before referral relapsed.One patient who received sulfadiazine,azithromycin and glucocorticoid therapy relapsed.One patient who received sulfadiazine therapy experienced relapse.Patients who received clindamycin and sulfadiazine or who received clindamycin only did not experience recurrence during the follow-up period.The best corrected visual acuity was improved in 6 eyes after inflammation resolved.Conclusions Primary active retinochoroiditis is the main form of OT in Hubei,China.Timely correct diagnosis on the basis of ocular characteristics and aetiological test results and effective treatment should be adopted to prevent poor visual outcomes and recurrence.展开更多
目的建立一套基于阴性检测结果的样本孔位内标CT值(Internal Control CT Value in negative samples,NEG-IC-CT)中位数(MD-IC)的实时质控方案,用于监测血站核酸实验室常规室内质控(IQC)未覆盖的核酸检测系统性能渐进性漂移等异常情况,...目的建立一套基于阴性检测结果的样本孔位内标CT值(Internal Control CT Value in negative samples,NEG-IC-CT)中位数(MD-IC)的实时质控方案,用于监测血站核酸实验室常规室内质控(IQC)未覆盖的核酸检测系统性能渐进性漂移等异常情况,并验证其可行性。方法回顾性收集本站万泰核酸检测系统运行期间,新、旧两套设备组四个试剂批次的54426份阴性样本的内标CT值。将每日NEG-IC-CT作为研究指标,用中位数绝对离差(MAD)计算控制限构建Median-MAD质控图,同时评估本方案对检测系统运行状态的监测能力。结果经统计分析,新旧设备间以及旧设备两个试剂批次间NEG-IC-CT值分布均存在显著差异(P<0.0001),新设备两个试剂批次间的NEG-IC-CT值分布差异不显著(P>0.05)。本方案识别出三类不同的异常情况,旧设备在O1、O2两个试剂批次出现的失控事件,提示设备老化引发性能衰减的可能;新设备B期试剂批号更换未及时记录,质控图出现了台阶型漂移;新设备A期后期,触发了系统的预警,提示混合使用剩余试剂、工作人员超长工作时间操作等行为可能存在质量风险。结论本研究构建的基于NEG-IC-CT值中位数的实时质控方案,其监控效能在本站核酸检测中已得到初步验证。该方案能有效察觉不同检测系统和不同试剂批次间的差异,对常规室内质控形成有效补充,该方案能为血站核酸检测过程的性能监控提供直观有效的评估方法。展开更多
As a novel micro-propulsion system for small satellites(from micro to nano),the ionic liquid electro spray propulsion system is a promising candidate.However,performance measurement and evaluation of the Ionic Liquid ...As a novel micro-propulsion system for small satellites(from micro to nano),the ionic liquid electro spray propulsion system is a promising candidate.However,performance measurement and evaluation of the Ionic Liquid Electrospray Thruster(ILET)is one of the most challenging issues for practical application,due to the difficulties in the development of a prototype and direct measurements of micro-thrust and small flow rate.To address this issue,a Modular Ionic Liquid Electrospray Thruster(MILET)prototype is constructed,and a diagnostic system for thrust and mass flow rate is specially developed based on an analytical balance method.With the ionic liquid 1-ethyl-3-methylimidazolium tetrafluoroborate as the propellant,a series of experiments is carried out on the MILET prototype under a wide operating condition through changing the applied voltage to control the thrust.Under different applied voltages,the thrust and the mass flow rate of the propellant are directly measured.The propulsive performance parameters of the thruster,such as thrust,specific impulse,thrust-to-power ratio,thruster efficiency,etc.,are comprehensively analyzed.Then,a performance comparison is made between the MILET and other representative ILETs.With a relatively low applied voltage ranging from 1550 V to 2000 V,the MILET achieves a quasi-constant specific impulse of 1263 s with the averaged thrust-to-power ratio of 65.2μN/W and thruster efficiency of 40.7%.The performance of ILET is also compared with other typical electric propulsions.The results demonstrate that the ILET exhibits an excellent ability of minimalization with high specific impulse and thruster efficiency,which guarantees a great superiority in micro propulsions.Finally,the ways to further improve the performance of ILET are discussed,which further confirms the potential prospect of ILET.The present result helps to advance the development and application of ILET.展开更多
AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from c...AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from cell culture media)reagent,and Total exosome isolation(from serum)reagent respectively.Identity and purity of the exosomes was confirmed by Nanosight?analysis,electron microscopy,and Western blots for CD63 marker.Exosomal RNA cargo was recovered with the Total exosome RNA and protein isolation kit.Finally,RNA was profiled using Bioanalyzer and quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR)methodology.RESULTS:Here we describe a novel approach for robust and scalable isolation of exosomes from cell culture media and serum,with subsequent isolation and analysis of RNA residing within these vesicles.The isolation procedure is completed in a fraction of the time,compared to the current standard protocols utilizing ultracentrifugation,and allows to recover fully intact exosomes in higher yields.Exosomes were found tocontain a very diverse RNA cargo,primarily short sequences 20-200 nt(such as mi RNA and fragments of m RNA),however longer RNA species were detected as well,including full-length 18S and 28S r RNA.CONCLUSION:We have successfully developed a set of reagents and a workflow allowing fast and efficient extraction of exosomes,followed by isolation of RNA and its analysis by q RT-PCR and other techniques.展开更多
OBJECTIVE To study the relationship between bFGF gene expression and proliferative activity in meningiomas. METHODS Thirty-seven samples of meningioma were examed using Northern hybridization for bFGF-gene expression ...OBJECTIVE To study the relationship between bFGF gene expression and proliferative activity in meningiomas. METHODS Thirty-seven samples of meningioma were examed using Northern hybridization for bFGF-gene expression and immunohistochemi- stry for bFGF protein expression and the Ki-67LI. RESULTS bFGF mRNA was detected in 22 meningiomas of Grade Ⅰ and in all samples of Grade Ⅱ and Ⅲ. Six of the Grade Ⅰ tumors were negative, giving an overall positive rate of 83.8 % for bFGF mRNA. Autoradiography was conducted using a thin scaning apparatus, bFGF mRNA expression compared to that for β-actin was 0.34±0.06 for Grade Ⅰ tumors compared to 0.82±0.12 for Grade Ⅱ and Ⅲ tumors. The difference was highly significant （P〈0.001）. The results of immunohistochemistry showed that the number of the positive cells for the bFGF protein was 29.7±7.6% in Grade Ⅰ tumors and 63.2±11.7 % in Grade Ⅱ plus Ⅲ, resulting in a significant difference between them （P〈0.001） . The Ki-67LI was found to be 2.8±1.1% in Grade Ⅰ and 6.5±1.3 % in Grade Ⅱ plus Ⅲ. The former was significantly lower than the later （P〈0.001）. CONCLUSION The expression of bFGF was correlated well with the malignancy of the meningiomas.展开更多
基金supported by the National Natural Science Foundation of China(Grants 62031013,62102339 and 62472124)the Guangdong Province Key Construction Discipline Scientific Research Capacity Improvement Project(Grant 2022ZDJS117)+1 种基金Shenzhen Colleges and Universities Stable Support Programme(Grant GXWD20220811170130002)Shenzhen Science and Technology Programme(Grant RCBS20221008093121052).
文摘Neural image compression(NIC)has shown remarkable rate-distortion(R-D)efficiency.However,the considerable computational and spatial complexity of most NIC methods presents deployment challenges on resource-constrained devices.We introduce a lightweight neural image compression framework designed to efficiently process both local and global information.In this framework,the convolutional branch extracts local information,whereas the frequency domain branch extracts global information.To capture global information without the high computational costs of dense pixel operations,such as attention mechanisms,Fourier transform is employed.This approach allows for the manipulation of global information in the frequency domain.Additionally,we employ feature shift operations as a strategy to acquire large receptive fields without any computational cost,thus circumventing the need for large kernel convolution.Our framework achieves a superior balance between ratedistortion performance and complexity.On varying resolution sets,our method not only achieves rate-distortion(R-D)performance on par with versatile video coding(VVC)intra and other state-of-the-art(SOTA)NIC methods but also exhibits the lowest computational requirements,with approximately 200 KMACs/pixel.The code will be available at https://github.com/baoyu2020/SFNIC.
基金supported by the National Natural Science Foundation of China(No.U22B2072)the Research Project of China Petroleum Science and Technology Innovation Fund(No.2025DQ02-0144).
文摘As the well drilling depth has broken through the 10,000 m in China,accurate measurements of downhole engineering parameters,such as annulus temperature and pressure for the whole wellbore,are significant in controlling potential downhole complexities.In this present work,a new micro-measurer is developed by integrating measurements of downhole temperature,pressure,magnetic field strength,and its own dynamic signals.The micro-measurer can flow with drilling fluid from the drillstring to the bottomhole and then float up back to the ground via the wellbore annulus.Compared with other downhole measurement tools that are fixedly connected to the drill string,its“measure-and-move-on”approach reduces the residence time in the high-temperature and high-pressure zone at the bottomhole;moreover,both the pressure and temperature at different well depth can be measured,thereby the temperature and pressure profiles of the whole wellbore can be constructed.In addition,the bluetooth low energy(BLE)technique is applied to offer the micro-measurer with the capability of wireless information transmission;while hydrodynamic optimization of the micro-measurer is carried out to design the structure of the micro-measurer,which can promote its recovery rate from downhole.In addition,an intelligent joint for releasing micro-measurers from the wellbore annulus is also proposed,aiming to overcome the limitation imposed by the nozzle on the size of the micro-measurer.Both the indoor experiments and the field tests have verified the feasibility of the newly designed micro-measurer,which is a key step for establishing a complete downhole internet of things(IoT)system to serve the intelligent drilling in the future.
基金Hubei Natural Science Foundation of China(No.2022CFB296).
文摘Objective The clinical features,disease course and visual outcomes of toxoplasmosis are less commonly reported in China than in other countries.To reduce misdiagnosis and improve visual function,the clinical characteristics,management and visual outcomes of 13 cases of ocular toxoplasmosis(OT)were described.Methods This retrospective study included 14 eyes of 13 patients who were diagnosed with OT in Hubei,China.The clinical characteristics,course of treatment and outcomes are presented.There were 7 males and 6 females.Results The main form of OT was retinochoroiditis with vitritis or anterior uveitis.Next-generation sequencing was applied to 3 eyes,and positive results were found in those eyes.Thirteen patients were positive for Toxoplasma gondii IgG antibodies,and 3 of them were also positive for IgM T.gondii antibodies.One patient with acquired immune deficiency syndrome was diagnosed with coinfection with OT and cytomegalovirus,as evidenced by an aqueous humor etiological test.Three patients were misdiagnosed with noninfectious uveitis.Recurrence occurred in 3 eyes during the follow-up periods.One patient who received vitreous implantation of Ozurdex therapy at another hospital before referral relapsed.One patient who received sulfadiazine,azithromycin and glucocorticoid therapy relapsed.One patient who received sulfadiazine therapy experienced relapse.Patients who received clindamycin and sulfadiazine or who received clindamycin only did not experience recurrence during the follow-up period.The best corrected visual acuity was improved in 6 eyes after inflammation resolved.Conclusions Primary active retinochoroiditis is the main form of OT in Hubei,China.Timely correct diagnosis on the basis of ocular characteristics and aetiological test results and effective treatment should be adopted to prevent poor visual outcomes and recurrence.
文摘目的建立一套基于阴性检测结果的样本孔位内标CT值(Internal Control CT Value in negative samples,NEG-IC-CT)中位数(MD-IC)的实时质控方案,用于监测血站核酸实验室常规室内质控(IQC)未覆盖的核酸检测系统性能渐进性漂移等异常情况,并验证其可行性。方法回顾性收集本站万泰核酸检测系统运行期间,新、旧两套设备组四个试剂批次的54426份阴性样本的内标CT值。将每日NEG-IC-CT作为研究指标,用中位数绝对离差(MAD)计算控制限构建Median-MAD质控图,同时评估本方案对检测系统运行状态的监测能力。结果经统计分析,新旧设备间以及旧设备两个试剂批次间NEG-IC-CT值分布均存在显著差异(P<0.0001),新设备两个试剂批次间的NEG-IC-CT值分布差异不显著(P>0.05)。本方案识别出三类不同的异常情况,旧设备在O1、O2两个试剂批次出现的失控事件,提示设备老化引发性能衰减的可能;新设备B期试剂批号更换未及时记录,质控图出现了台阶型漂移;新设备A期后期,触发了系统的预警,提示混合使用剩余试剂、工作人员超长工作时间操作等行为可能存在质量风险。结论本研究构建的基于NEG-IC-CT值中位数的实时质控方案,其监控效能在本站核酸检测中已得到初步验证。该方案能有效察觉不同检测系统和不同试剂批次间的差异,对常规室内质控形成有效补充,该方案能为血站核酸检测过程的性能监控提供直观有效的评估方法。
基金co-supported by NSAF,China(No.U1730134)the National Natural Science Foundation of China(No.11772310)+1 种基金the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University,China(No.CX202034)the Innovation and Entrepreneurship Fund of the Student Affairs Department of Northwestern Polytechnical University,China(No.2021-CXCY-005)。
文摘As a novel micro-propulsion system for small satellites(from micro to nano),the ionic liquid electro spray propulsion system is a promising candidate.However,performance measurement and evaluation of the Ionic Liquid Electrospray Thruster(ILET)is one of the most challenging issues for practical application,due to the difficulties in the development of a prototype and direct measurements of micro-thrust and small flow rate.To address this issue,a Modular Ionic Liquid Electrospray Thruster(MILET)prototype is constructed,and a diagnostic system for thrust and mass flow rate is specially developed based on an analytical balance method.With the ionic liquid 1-ethyl-3-methylimidazolium tetrafluoroborate as the propellant,a series of experiments is carried out on the MILET prototype under a wide operating condition through changing the applied voltage to control the thrust.Under different applied voltages,the thrust and the mass flow rate of the propellant are directly measured.The propulsive performance parameters of the thruster,such as thrust,specific impulse,thrust-to-power ratio,thruster efficiency,etc.,are comprehensively analyzed.Then,a performance comparison is made between the MILET and other representative ILETs.With a relatively low applied voltage ranging from 1550 V to 2000 V,the MILET achieves a quasi-constant specific impulse of 1263 s with the averaged thrust-to-power ratio of 65.2μN/W and thruster efficiency of 40.7%.The performance of ILET is also compared with other typical electric propulsions.The results demonstrate that the ILET exhibits an excellent ability of minimalization with high specific impulse and thruster efficiency,which guarantees a great superiority in micro propulsions.Finally,the ways to further improve the performance of ILET are discussed,which further confirms the potential prospect of ILET.The present result helps to advance the development and application of ILET.
文摘AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from cell culture media)reagent,and Total exosome isolation(from serum)reagent respectively.Identity and purity of the exosomes was confirmed by Nanosight?analysis,electron microscopy,and Western blots for CD63 marker.Exosomal RNA cargo was recovered with the Total exosome RNA and protein isolation kit.Finally,RNA was profiled using Bioanalyzer and quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR)methodology.RESULTS:Here we describe a novel approach for robust and scalable isolation of exosomes from cell culture media and serum,with subsequent isolation and analysis of RNA residing within these vesicles.The isolation procedure is completed in a fraction of the time,compared to the current standard protocols utilizing ultracentrifugation,and allows to recover fully intact exosomes in higher yields.Exosomes were found tocontain a very diverse RNA cargo,primarily short sequences 20-200 nt(such as mi RNA and fragments of m RNA),however longer RNA species were detected as well,including full-length 18S and 28S r RNA.CONCLUSION:We have successfully developed a set of reagents and a workflow allowing fast and efficient extraction of exosomes,followed by isolation of RNA and its analysis by q RT-PCR and other techniques.
文摘OBJECTIVE To study the relationship between bFGF gene expression and proliferative activity in meningiomas. METHODS Thirty-seven samples of meningioma were examed using Northern hybridization for bFGF-gene expression and immunohistochemi- stry for bFGF protein expression and the Ki-67LI. RESULTS bFGF mRNA was detected in 22 meningiomas of Grade Ⅰ and in all samples of Grade Ⅱ and Ⅲ. Six of the Grade Ⅰ tumors were negative, giving an overall positive rate of 83.8 % for bFGF mRNA. Autoradiography was conducted using a thin scaning apparatus, bFGF mRNA expression compared to that for β-actin was 0.34±0.06 for Grade Ⅰ tumors compared to 0.82±0.12 for Grade Ⅱ and Ⅲ tumors. The difference was highly significant （P〈0.001）. The results of immunohistochemistry showed that the number of the positive cells for the bFGF protein was 29.7±7.6% in Grade Ⅰ tumors and 63.2±11.7 % in Grade Ⅱ plus Ⅲ, resulting in a significant difference between them （P〈0.001） . The Ki-67LI was found to be 2.8±1.1% in Grade Ⅰ and 6.5±1.3 % in Grade Ⅱ plus Ⅲ. The former was significantly lower than the later （P〈0.001）. CONCLUSION The expression of bFGF was correlated well with the malignancy of the meningiomas.
