Anti-inflammatory peptides that derived from food attract more and more attention due to their wide range of sources and easy absorption.In this study,the aim was to identify and characterize anti-inflammatory peptide...Anti-inflammatory peptides that derived from food attract more and more attention due to their wide range of sources and easy absorption.In this study,the aim was to identify and characterize anti-inflammatory peptides from yak bone collagen.The prepared yak bone collagen peptides(YBCPs)was separated,purified and identified through ultrafiltration,reverse-phase high-performance liquid chromatography(RP-HPLC),and nano-liquid chromatography-tandem mass spectrometry(nano LC-MS/MS)successively.Finally,a total of 115 peptides were identified.After that,12 peptides were screened out based on the predicted biological activity score.Molecular docking results indicated that 6 peptides(GPAGPSGPAGK,GPAGPSGPAGKDGR,GPSGPQGIR,GPAGPQGPR,GEAGPAGPAGPAGPR,and GEGGPQGPR)could successfully interact with the key factors in the nuclear factor kappa-B(NF-κB)signaling pathway and nitric oxide(NO)production due to form various bonds such as salt bridge,conventional hydrogen bond,and carbon-hydrogen bond.The anti-inflammatory effects of these 6 peptides were further verified by in vitro cell test.Results indicated that the 6 peptides might play an anti-inflammatory role by regulating different proteins in the NF-κB signaling pathway and NO production.This study would further promote the application of yak bone collagen in health food.展开更多
Cancers of the digestive system are major contributors to global cancer-associated morbidity and mortality,accounting for 35%of annual cases of cancer deaths.The etiologies,molecular features,and therapeutic managemen...Cancers of the digestive system are major contributors to global cancer-associated morbidity and mortality,accounting for 35%of annual cases of cancer deaths.The etiologies,molecular features,and therapeutic management of these cancer entities are highly heterogeneous and complex.Over the last decade,genomic and functional studies have provided unprecedented insights into the biology of digestive cancers,identifying genetic drivers of tumor progression and key interaction points of tumor cells with the immune system.This knowledge is continuously translated into novel treatment concepts and targets,which are dynamically reshaping the therapeutic landscape of these tumors.In this review,we provide a concise overview of the etiology and molecular pathology of the six most common cancers of the digestive system,including esophageal,gastric,biliary tract,pancreatic,hepatocellular,and colorectal cancers.We comprehensively describe the current stage-dependent pharmacological management of these malignancies,including chemo-,targeted,and immunotherapy.For each cancer entity,we provide an overview of recent therapeutic advancements and research progress.Finally,we describe how novel insights into tumor heterogeneity and immune evasion deepen our understanding of therapy resistance and provide an outlook on innovative therapeutic strategies that will shape the future management of digestive cancers,including CAR-T cell therapy,novel antibody-drug conjugates and targeted therapies.展开更多
基金This work was supported by Postdoctoral Science Foundation of Jiangsu Province(2021K269B)the National Key Research&Developmental Program of China(2021YFC2100300)+1 种基金China Agriculture Research System of Finance and Agriculture Ministry(No.CARS-11-HNSHY)Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(No.1630052021012,1630052020023,1630052020024).
文摘Anti-inflammatory peptides that derived from food attract more and more attention due to their wide range of sources and easy absorption.In this study,the aim was to identify and characterize anti-inflammatory peptides from yak bone collagen.The prepared yak bone collagen peptides(YBCPs)was separated,purified and identified through ultrafiltration,reverse-phase high-performance liquid chromatography(RP-HPLC),and nano-liquid chromatography-tandem mass spectrometry(nano LC-MS/MS)successively.Finally,a total of 115 peptides were identified.After that,12 peptides were screened out based on the predicted biological activity score.Molecular docking results indicated that 6 peptides(GPAGPSGPAGK,GPAGPSGPAGKDGR,GPSGPQGIR,GPAGPQGPR,GEAGPAGPAGPAGPR,and GEGGPQGPR)could successfully interact with the key factors in the nuclear factor kappa-B(NF-κB)signaling pathway and nitric oxide(NO)production due to form various bonds such as salt bridge,conventional hydrogen bond,and carbon-hydrogen bond.The anti-inflammatory effects of these 6 peptides were further verified by in vitro cell test.Results indicated that the 6 peptides might play an anti-inflammatory role by regulating different proteins in the NF-κB signaling pathway and NO production.This study would further promote the application of yak bone collagen in health food.
基金supported by DFG-SFB1324.MPE is supported by the DFG(RTG 2727,Project B1.3.,445549683)the Dr.Hans und Lore Graf Stiftung,Germany.JB is supported by the Hector Foundation II.ML and LD were supported by the Clinician Scientist Program ICON of the Medical Faculty Mannheim.We apologize to all colleagues whose work was not cited due to space constraints.
文摘Cancers of the digestive system are major contributors to global cancer-associated morbidity and mortality,accounting for 35%of annual cases of cancer deaths.The etiologies,molecular features,and therapeutic management of these cancer entities are highly heterogeneous and complex.Over the last decade,genomic and functional studies have provided unprecedented insights into the biology of digestive cancers,identifying genetic drivers of tumor progression and key interaction points of tumor cells with the immune system.This knowledge is continuously translated into novel treatment concepts and targets,which are dynamically reshaping the therapeutic landscape of these tumors.In this review,we provide a concise overview of the etiology and molecular pathology of the six most common cancers of the digestive system,including esophageal,gastric,biliary tract,pancreatic,hepatocellular,and colorectal cancers.We comprehensively describe the current stage-dependent pharmacological management of these malignancies,including chemo-,targeted,and immunotherapy.For each cancer entity,we provide an overview of recent therapeutic advancements and research progress.Finally,we describe how novel insights into tumor heterogeneity and immune evasion deepen our understanding of therapy resistance and provide an outlook on innovative therapeutic strategies that will shape the future management of digestive cancers,including CAR-T cell therapy,novel antibody-drug conjugates and targeted therapies.
