Objective:To investigate the characteristics of PEGylated mucin and its potential usage. Methods:Mucin was extracted from giant African land snails and PECylated mucin was prepared with different ratios of PEG 2000-Mw...Objective:To investigate the characteristics of PEGylated mucin and its potential usage. Methods:Mucin was extracted from giant African land snails and PECylated mucin was prepared with different ratios of PEG 2000-Mwt and mucin（1:1,0:1,2:1,1:3 and 3:1 to form batch A-E） using solvent technique.The physicochemical properties of mucin were identified and the solubility of mucin was assessed.The thermal properties of PEGylated mucin were measured by differential scanning calorimetry（DSC）.Results:Carbohydrates,proteins and trace amounts of fats were present in snail mucin.The mucin powder was water-soluble at 30℃and more water-soluble at 35℃but not soluble;in acetone,ethanol,0.1 M NaOH,0.1M H<sub>2</sub>SO<sub>4</sub> and 0.1 NH<sub>4</sub>OH was water-soluble.The melting point T<sub>m</sub> ranged from 58.58℃to 61.17℃, crystallization temperature Tc 37.08℃to 39.83℃,and glass transition temperature Tg 126.85℃to 138.39℃.The variation in T<sub>m</sub>,T<sub>c</sub>,and T<sub>g</sub> with the composition in the PEGylaton showed that an interaction between PEG and mucin occured.Conclusions:This result can serve as a basis for further evaluation of the PEGylation method and be used for drug delivery.展开更多
Objective:To investigate the immunological effect of Vernonia amygdalina(V.amygdalina) leaf extract and immunace on HIV infected patients taking highly active antiretrwiral therapy. Methods:Fresh V.amygdalina leaves w...Objective:To investigate the immunological effect of Vernonia amygdalina(V.amygdalina) leaf extract and immunace on HIV infected patients taking highly active antiretrwiral therapy. Methods:Fresh V.amygdalina leaves were collected within Nsukka area in Enugu State.The leaves were rinsed with distilled water.Two handful of cleaned fresh leaves were soaked in 200 mL water and squeezed gently by hand to a mixture.Clients were divided into four groups and each group was given different combination.They took the medication for four weeks.The immune effect was tested against marketed immune booster in some retroviral clients.Results:The mean absolute CD4 count was increased in the client who took the extract or supplement.And the clients who took both the extract and supplement had a greater increase in the CD4 count.The increased CD4 was significant as compared with the control group(P<0.05).The skin rashes were also improved in the entire groups.Conclusions:It can be concluded that the aqueous extract of V.amygdalina and immunace or both have immunological effect on HIV infected patients. Therefore,we suggest that the V.amygdalina extract or immunace or both could be used as adjuvant in the management of HIV/AIDS clients.展开更多
文摘Objective:To investigate the characteristics of PEGylated mucin and its potential usage. Methods:Mucin was extracted from giant African land snails and PECylated mucin was prepared with different ratios of PEG 2000-Mwt and mucin（1:1,0:1,2:1,1:3 and 3:1 to form batch A-E） using solvent technique.The physicochemical properties of mucin were identified and the solubility of mucin was assessed.The thermal properties of PEGylated mucin were measured by differential scanning calorimetry（DSC）.Results:Carbohydrates,proteins and trace amounts of fats were present in snail mucin.The mucin powder was water-soluble at 30℃and more water-soluble at 35℃but not soluble;in acetone,ethanol,0.1 M NaOH,0.1M H<sub>2</sub>SO<sub>4</sub> and 0.1 NH<sub>4</sub>OH was water-soluble.The melting point T<sub>m</sub> ranged from 58.58℃to 61.17℃, crystallization temperature Tc 37.08℃to 39.83℃,and glass transition temperature Tg 126.85℃to 138.39℃.The variation in T<sub>m</sub>,T<sub>c</sub>,and T<sub>g</sub> with the composition in the PEGylaton showed that an interaction between PEG and mucin occured.Conclusions:This result can serve as a basis for further evaluation of the PEGylation method and be used for drug delivery.
文摘Objective:To investigate the immunological effect of Vernonia amygdalina(V.amygdalina) leaf extract and immunace on HIV infected patients taking highly active antiretrwiral therapy. Methods:Fresh V.amygdalina leaves were collected within Nsukka area in Enugu State.The leaves were rinsed with distilled water.Two handful of cleaned fresh leaves were soaked in 200 mL water and squeezed gently by hand to a mixture.Clients were divided into four groups and each group was given different combination.They took the medication for four weeks.The immune effect was tested against marketed immune booster in some retroviral clients.Results:The mean absolute CD4 count was increased in the client who took the extract or supplement.And the clients who took both the extract and supplement had a greater increase in the CD4 count.The increased CD4 was significant as compared with the control group(P<0.05).The skin rashes were also improved in the entire groups.Conclusions:It can be concluded that the aqueous extract of V.amygdalina and immunace or both have immunological effect on HIV infected patients. Therefore,we suggest that the V.amygdalina extract or immunace or both could be used as adjuvant in the management of HIV/AIDS clients.
