In nanotechnology,using of metallic nanoparticles(MNPs)has obtained major attention for over a century because of their unique properties in nano scale.In this paper,the effect of three factors(incubation time,concent...In nanotechnology,using of metallic nanoparticles(MNPs)has obtained major attention for over a century because of their unique properties in nano scale.In this paper,the effect of three factors(incubation time,concentrations of copper sulfate and glucose)on the biosynthesis of copper(II)oxide nanoparticles(CuO NPs)by Halomonas elongata IBRC-M 10214 and the antibacterial activity of nanoparticles were evaluated.Experiment design and analysis of Taguchi method were carried out by Qualitek-4 software.Effect of CuSO4 concentration(1.4,2.8 and 5.6 mM),incubation time(48,72 and 96 h)as three different levels was measured as three major factors in the biosynthesis of metallic nanoparticles.Synthesized CuO nanoparticles were characterized by utilizing ultraviolet-visible(UV-Vis)spectroscopy,X-ray diffraction(XRD),Fourier transform infra-red(FT-IR)spectroscopy and field emission scanning electron microscope(FESEM)techniques.Among the three factors,results illustrated that considerable effect was related to CuSO4 concentration.These analyses demonstrated that the average CuO nanoparticles crystalline size was 57-79 nm with rectangular shape.Also,for evaluating of antibacterial effects,maximum zone of inhibition,two important multidrug resistant pathogenesis bacteria,Escherichia coli ATCC 25922,and Staphylococcus aureus ATCC 43300 were used.Antibacterial assay of CuO nanoparticles showed antibacterial activity toward the pathogenic bacterial strains of E.coli by 5 mm and S.aureus by 5.5 mm for maximum zone of inhibition.In conclusion,this study presented simple,low expensive,eco-friendly and high productivity in the fabrication of CuO nanoparticles.In addition,these metallic nanoparticles had antibacterial effect that could be usable in medicinal aspect for fighting against prominent pathogen bacteria such as E.coli ATCC 25922 and S.aureus ATCC 43300.展开更多
Urinary tract infections(UTIs)are the reason for 15-70%of urinary stone disease(USD).Many diabetic patients suffer from USD resulting from bacterial infections by multi drug resistant(MDR)bacteria such as Staphylococc...Urinary tract infections(UTIs)are the reason for 15-70%of urinary stone disease(USD).Many diabetic patients suffer from USD resulting from bacterial infections by multi drug resistant(MDR)bacteria such as Staphylococcus aureus ATCC 43300.These bacteria can enhance struvite stones’formation in diabetic patients with UTIs.In this regard,hyperglycemia,proteinuria,and using of metformin drug may be important factors.Three parameters including glucose,metformin,and protein with their three levels of concentrations were determined by experimental design of Taguchi method to obtain optimized formation of struvite stones.Artificial urine medium was utilized for simulation of natural human urine.Scanning electron microscopy(SEM)and energy dispersive X-ray analysis(EDAX)techniques were applied for evaluation of morphology and elemental compositions of formed calcium phosphate.This study showed highest concentration of struvite stones(0.512 g/L)under interaction conditions of 3×2×2 levels respectively for glucose,metformin,and protein.Results of SEM and EDAX analyses demonstrated clumped crystallite property and flower-like nanocomposites(NCs)of struvite stones with contribution of calcium(160.8)and phosphate(131.4)elements.It may be concluded from this investigation that therapy of MDR bacteria,hyperglycemia,and proteinuria can decrease urinary stone formation in diabetic patients by having UTIs.展开更多
文摘In nanotechnology,using of metallic nanoparticles(MNPs)has obtained major attention for over a century because of their unique properties in nano scale.In this paper,the effect of three factors(incubation time,concentrations of copper sulfate and glucose)on the biosynthesis of copper(II)oxide nanoparticles(CuO NPs)by Halomonas elongata IBRC-M 10214 and the antibacterial activity of nanoparticles were evaluated.Experiment design and analysis of Taguchi method were carried out by Qualitek-4 software.Effect of CuSO4 concentration(1.4,2.8 and 5.6 mM),incubation time(48,72 and 96 h)as three different levels was measured as three major factors in the biosynthesis of metallic nanoparticles.Synthesized CuO nanoparticles were characterized by utilizing ultraviolet-visible(UV-Vis)spectroscopy,X-ray diffraction(XRD),Fourier transform infra-red(FT-IR)spectroscopy and field emission scanning electron microscope(FESEM)techniques.Among the three factors,results illustrated that considerable effect was related to CuSO4 concentration.These analyses demonstrated that the average CuO nanoparticles crystalline size was 57-79 nm with rectangular shape.Also,for evaluating of antibacterial effects,maximum zone of inhibition,two important multidrug resistant pathogenesis bacteria,Escherichia coli ATCC 25922,and Staphylococcus aureus ATCC 43300 were used.Antibacterial assay of CuO nanoparticles showed antibacterial activity toward the pathogenic bacterial strains of E.coli by 5 mm and S.aureus by 5.5 mm for maximum zone of inhibition.In conclusion,this study presented simple,low expensive,eco-friendly and high productivity in the fabrication of CuO nanoparticles.In addition,these metallic nanoparticles had antibacterial effect that could be usable in medicinal aspect for fighting against prominent pathogen bacteria such as E.coli ATCC 25922 and S.aureus ATCC 43300.
文摘Urinary tract infections(UTIs)are the reason for 15-70%of urinary stone disease(USD).Many diabetic patients suffer from USD resulting from bacterial infections by multi drug resistant(MDR)bacteria such as Staphylococcus aureus ATCC 43300.These bacteria can enhance struvite stones’formation in diabetic patients with UTIs.In this regard,hyperglycemia,proteinuria,and using of metformin drug may be important factors.Three parameters including glucose,metformin,and protein with their three levels of concentrations were determined by experimental design of Taguchi method to obtain optimized formation of struvite stones.Artificial urine medium was utilized for simulation of natural human urine.Scanning electron microscopy(SEM)and energy dispersive X-ray analysis(EDAX)techniques were applied for evaluation of morphology and elemental compositions of formed calcium phosphate.This study showed highest concentration of struvite stones(0.512 g/L)under interaction conditions of 3×2×2 levels respectively for glucose,metformin,and protein.Results of SEM and EDAX analyses demonstrated clumped crystallite property and flower-like nanocomposites(NCs)of struvite stones with contribution of calcium(160.8)and phosphate(131.4)elements.It may be concluded from this investigation that therapy of MDR bacteria,hyperglycemia,and proteinuria can decrease urinary stone formation in diabetic patients by having UTIs.
