Biotechnology has revolutionized agriculture through innovations such as genetically modified(GM)technology and CRISPR/Cas9 genome editing.In this review,a comparative analysis of these methods that addresses the conc...Biotechnology has revolutionized agriculture through innovations such as genetically modified(GM)technology and CRISPR/Cas9 genome editing.In this review,a comparative analysis of these methods that addresses the concerns regarding the oversimplification of the notion that the CRISPR-based editing approach is equivalent to traditional GM approaches is offered.Since the 1990s,despite the potential benefits for crop improvement and food security,controversies have arisen around recombinant technology and the introduction of GM products due to perceived environmental and health concerns.In contrast,the recent emergence of the CRISPR/Cas systemas a precise genome editing tool has garnered relatively less public resistance.In this review,the reasons for these contrasting findings are explored to shed light on the distinct characteristics inherent to each approach.Additionally,we conduct a bibliographic analysis to examine the shift in research priorities between the two technological eras,followed by a comprehensive comparison of the two approaches,to enable a better understanding of the potential synergies that can be achieved between agricultural biotechnology and conventional techniques for enhancing modern agriculture.A nuanced understanding of these technologies is crucial for informed decision-making,responsible implementation,and addressing the concerns surrounding agricultural biotechnology.展开更多
Abiotic stresses such as drought,heat,salinity,and heavy metal contamination severely affect global agricultural productivity.Between 2005 and 2015,droughts caused losses of approximately USD 29 billion in developing ...Abiotic stresses such as drought,heat,salinity,and heavy metal contamination severely affect global agricultural productivity.Between 2005 and 2015,droughts caused losses of approximately USD 29 billion in developing countries,and from 2008 to 2018,droughts accounted for over 34%of crop and livestock yield losses,totaling about USD 37 billion.To support the growing human population,agricultural output must increase substantially,necessitating a 60%–100%rise in crop productivity to meet the escalating demand.To address environmental challenges,organic,inorganic,and microbial biostimulants are increasingly employed to enhance plant resilience through various morphological,physiological,and biochemical modifications.Plant biostimulants enhance plant resilience under abiotic stress through mechanisms such as abscisic acid signaling modulation,which regulates stomatal closure to reduce water loss during drought and heat stress.Additionally,they aid in scavenging reactive oxygen species and stabilizing ion channels,mitigating oxidative damage,and maintaining ionic balance under stress conditions such as salinity.This review summarizes recent advancements in applying these biostimulants,focusing on their roles in triggering morphological,physiological,biochemical,and molecular changes that collectively enhance plant resilience under stress conditions.It also includes a bibliometric analysis of all articles published on biostimulants from 2019 to 2024 and explores future research directions.Emphasis was placed on optimizing biostimulant formulations and understanding their synergistic effects to maximize their efficacy under various stress conditions.By integrating biostimulants into agricultural practices,we can adopt a sustainable strategy to safeguard crop productivity in the face of climate change and environmental stressors.展开更多
基金supported by the by the Fundamental Research Grant Scheme(Grant No.FRGS/1/2023/STG03/UM/02/2)Universiti Malaya RU Fund(Grant No.ST087-2022).
文摘Biotechnology has revolutionized agriculture through innovations such as genetically modified(GM)technology and CRISPR/Cas9 genome editing.In this review,a comparative analysis of these methods that addresses the concerns regarding the oversimplification of the notion that the CRISPR-based editing approach is equivalent to traditional GM approaches is offered.Since the 1990s,despite the potential benefits for crop improvement and food security,controversies have arisen around recombinant technology and the introduction of GM products due to perceived environmental and health concerns.In contrast,the recent emergence of the CRISPR/Cas systemas a precise genome editing tool has garnered relatively less public resistance.In this review,the reasons for these contrasting findings are explored to shed light on the distinct characteristics inherent to each approach.Additionally,we conduct a bibliographic analysis to examine the shift in research priorities between the two technological eras,followed by a comprehensive comparison of the two approaches,to enable a better understanding of the potential synergies that can be achieved between agricultural biotechnology and conventional techniques for enhancing modern agriculture.A nuanced understanding of these technologies is crucial for informed decision-making,responsible implementation,and addressing the concerns surrounding agricultural biotechnology.
文摘Abiotic stresses such as drought,heat,salinity,and heavy metal contamination severely affect global agricultural productivity.Between 2005 and 2015,droughts caused losses of approximately USD 29 billion in developing countries,and from 2008 to 2018,droughts accounted for over 34%of crop and livestock yield losses,totaling about USD 37 billion.To support the growing human population,agricultural output must increase substantially,necessitating a 60%–100%rise in crop productivity to meet the escalating demand.To address environmental challenges,organic,inorganic,and microbial biostimulants are increasingly employed to enhance plant resilience through various morphological,physiological,and biochemical modifications.Plant biostimulants enhance plant resilience under abiotic stress through mechanisms such as abscisic acid signaling modulation,which regulates stomatal closure to reduce water loss during drought and heat stress.Additionally,they aid in scavenging reactive oxygen species and stabilizing ion channels,mitigating oxidative damage,and maintaining ionic balance under stress conditions such as salinity.This review summarizes recent advancements in applying these biostimulants,focusing on their roles in triggering morphological,physiological,biochemical,and molecular changes that collectively enhance plant resilience under stress conditions.It also includes a bibliometric analysis of all articles published on biostimulants from 2019 to 2024 and explores future research directions.Emphasis was placed on optimizing biostimulant formulations and understanding their synergistic effects to maximize their efficacy under various stress conditions.By integrating biostimulants into agricultural practices,we can adopt a sustainable strategy to safeguard crop productivity in the face of climate change and environmental stressors.
