The clustered regularly interspaced short palindromic repeat(CRISPR)-Caspase(Cas)system acts as a natural defense of bacteria against invasion by mobile genetic elements,such as plasmids,transposons,and bacteriophages...The clustered regularly interspaced short palindromic repeat(CRISPR)-Caspase(Cas)system acts as a natural defense of bacteria against invasion by mobile genetic elements,such as plasmids,transposons,and bacteriophages.The review discusses the different classes and types of CRISPR-Cas systems in terms of principles of their action,limitations,and future perspectives.Also,the role of the CRISPR-Cas system as a new arsenal in fighting multidrug-resistant pathogens and clinically relevant pathogenic viruses and up-to-date clinical trials have been discussed and highlighted.Moreover,the utilization mode,regulation,and the link of CRISPR-Cas to quorum sensing for targeting cell chromosome or antimicrobial-resistant gene(s)of some clinically relevant pathogens,including Klebsiella pneumoniae,Escherichia coli,and Pseudomonas aeruginosa have been discussed.The information provided in this review can act as a platform for researchers for the future use of the CRISPR-Cas system as a smart antimicrobial agent for combating infections caused by life-threatening pathogens,particularly those with limited therapeutic options.展开更多
基金supported by the Large Research Group Project of the Deanship of Scientific Research at King Khalid University,Abha,Saudi Arabia(Project No.R.G.P.2/297/44).
文摘The clustered regularly interspaced short palindromic repeat(CRISPR)-Caspase(Cas)system acts as a natural defense of bacteria against invasion by mobile genetic elements,such as plasmids,transposons,and bacteriophages.The review discusses the different classes and types of CRISPR-Cas systems in terms of principles of their action,limitations,and future perspectives.Also,the role of the CRISPR-Cas system as a new arsenal in fighting multidrug-resistant pathogens and clinically relevant pathogenic viruses and up-to-date clinical trials have been discussed and highlighted.Moreover,the utilization mode,regulation,and the link of CRISPR-Cas to quorum sensing for targeting cell chromosome or antimicrobial-resistant gene(s)of some clinically relevant pathogens,including Klebsiella pneumoniae,Escherichia coli,and Pseudomonas aeruginosa have been discussed.The information provided in this review can act as a platform for researchers for the future use of the CRISPR-Cas system as a smart antimicrobial agent for combating infections caused by life-threatening pathogens,particularly those with limited therapeutic options.
