Twenty lactating cows of two to five parity having an average live weight of 290.00 to 330.00 kg and an average milk production of 4.54 kg/head/day to 4.66 kg/head/day were selected from the Central Cattle breeding St...Twenty lactating cows of two to five parity having an average live weight of 290.00 to 330.00 kg and an average milk production of 4.54 kg/head/day to 4.66 kg/head/day were selected from the Central Cattle breeding Station and Dairy Farm. The four dietary treatment were： SlooTo （Straw 100： Triticale 0 as control）; $5oT5o （Straw 50： Triticale 50）; $25T75 （Straw 25： Triticale 75） and SoTloo （Straw 0： Triticale 100）. It was observed that the roughage dry matter intake （DMI） （2.61 ~ 0.07 kg） in percent live weight was significantly （P 〈 0.05） higher in $25T75 and the lowest （1.76 ~ 0.018 kg） in the control group （SlooTo） and the differences were significant among the dietary treatments except $5oT5o and SoTloo. The total DMI was significantly （P 〈 0.05） higher in SoT^oo （13.36 i 0.13 kg） followed by $25T75, $5oT5o and SlooTo, respectively. The digestibility of crude protein （CP） was slightly higher in $25T75 （75.48 ~ 0.96） compared to SoTtoo （75.31 ~ 1.45） and the difference was non-significant （P 〉 0.05）. Milk production was significantly （P 〈 0.05） the highest in $5oT5o and the lowest in SlooTo followed by SoTloo, $25T75, respectively. The percent increased in milk yield was also the highest in $5oT5o （67.68%） and the lowest in SlooTo （28.85%）. The 4% fat corrected milk was also significantly （P 〈 0.05） higher in $5oT5o followed by SoTloo, $25T75 and SlooTo, respectively. Therefore, it can be concluded that triticale and straw at a ratio of 50：50 may be fed for better production performance of dairy cows.展开更多
Two experiments were carried out to investigate the effect of dietary supple-mentation of urea molasses multi-nutrient cake (UMMC) on productive and reproductive performances of rabbit. In Experiment-I, 80 rabbit does...Two experiments were carried out to investigate the effect of dietary supple-mentation of urea molasses multi-nutrient cake (UMMC) on productive and reproductive performances of rabbit. In Experiment-I, 80 rabbit does (age 24 weeks) were randomly assigned into four dietary treatment groups (T0, T1, T2 and T3) to investigate the effect of UMMC on the reproductive performances whereas in Experiment-II, 112 young rabbits (age 5 weeks) were also randomly assigned into another four dietary treatment groups to investigate the effect of UMMC on the productive performance. The rabbits of four dietary treatment groups (T0, T1, T2 and T3) in both experiments were fed 0%, 4%, 6% and 8% urea containing UMMC, respectively. The results revealed that the dietary supplementation of UMMC increased (P < 0.05) live weight of pregnant does, conception rate, litter size, and litter weight and individual kit weight at weaning. On the other hand, UMMC supplementation reduced (P < 0.05) the kit mortality, though it did not affect the gestation period, litter size, litter weight and individual kit weight at birth. Dietary UMMC supplementation also improved (P < 0.05) the productive performances of growing rabbits in terms of live weight gain, feed conversion ratio and performance index by almost same amount of feed intake. On the contrary, blood parameters (Hb, PCV and ESR) were not affected by the UMMC except for red blood cell and white blood cell counts, but were within their normal range indicating the safe use of UMMC. UMMC also increased (P < 0.05) the fecal bacterial population. Production cost of UMMC was around 0.19 USD less in each kg of feed than the commercial pellet. In both of the experiments rabbits fed 6% urea containing UMMC showed best result than control and other treatment groups as well.展开更多
Background: Mammalian ovaries contain follicles containing an oocyte enclosed by layers of granulosa cells (GC). Follicle growth and oocyte maturation are largely dependent on GC numbers and viability, but there is no...Background: Mammalian ovaries contain follicles containing an oocyte enclosed by layers of granulosa cells (GC). Follicle growth and oocyte maturation are largely dependent on GC numbers and viability, but there is no established, reliable method for assessing the number of viable GC within an isolated follicle. Methods: Centrifugation conditions and the Trypan Blue (TB) Exclusion assay were optimised for low cell densities compatible with the numbers of GC in follicles. Mouse ovarian follicles were disaggregated to produce a single cell suspension of GC which were examined by TB (n = 4), but also by crystal violet assay in a 96-well plate format after 24 h in vitro (n = 3). GC viability in vitro was characterised further by using enzyme-linked immunoassays to quantify GC production of anti-Mullerian hormone (AMH) and estrogen. Results: The centrifugation and low cell density TB protocol could accurately measure the viability of 78 GC in 10 μL, with an intra-assay coefficient of variation (CoV) 22%, and inter-assay CoV 7%. The best follicle disaggregation method (30 min 37°C exposure to 2 mg/mL collagenase prior to 30 min exposure to 0.025% hyaluronidase) yielded (656 ±87) GC per antral follicle of which 82% ±5% were viable. Culturing 312 - 20,000 GC per well for 24 hours and assessing viability by crystal violet assay generated a linear correlation between OD value and viable GC number (R2 = 0.98) and estrogen concentration per well (R2 = 0.92). 20,000 GC per well produced 143 ±16 pg/mL estrogen during 24 hours in vitro, but no detectable AMH. Conclusion: This is the first report describing the isolation of viable, estrogen-producing GC from murine follicles, and their subsequent culture. These procedures are transferrable to other species including humans and can be applied to screening the reproductive toxicity of pharmaceutical agents.展开更多
文摘Twenty lactating cows of two to five parity having an average live weight of 290.00 to 330.00 kg and an average milk production of 4.54 kg/head/day to 4.66 kg/head/day were selected from the Central Cattle breeding Station and Dairy Farm. The four dietary treatment were： SlooTo （Straw 100： Triticale 0 as control）; $5oT5o （Straw 50： Triticale 50）; $25T75 （Straw 25： Triticale 75） and SoTloo （Straw 0： Triticale 100）. It was observed that the roughage dry matter intake （DMI） （2.61 ~ 0.07 kg） in percent live weight was significantly （P 〈 0.05） higher in $25T75 and the lowest （1.76 ~ 0.018 kg） in the control group （SlooTo） and the differences were significant among the dietary treatments except $5oT5o and SoTloo. The total DMI was significantly （P 〈 0.05） higher in SoT^oo （13.36 i 0.13 kg） followed by $25T75, $5oT5o and SlooTo, respectively. The digestibility of crude protein （CP） was slightly higher in $25T75 （75.48 ~ 0.96） compared to SoTtoo （75.31 ~ 1.45） and the difference was non-significant （P 〉 0.05）. Milk production was significantly （P 〈 0.05） the highest in $5oT5o and the lowest in SlooTo followed by SoTloo, $25T75, respectively. The percent increased in milk yield was also the highest in $5oT5o （67.68%） and the lowest in SlooTo （28.85%）. The 4% fat corrected milk was also significantly （P 〈 0.05） higher in $5oT5o followed by SoTloo, $25T75 and SlooTo, respectively. Therefore, it can be concluded that triticale and straw at a ratio of 50：50 may be fed for better production performance of dairy cows.
文摘Two experiments were carried out to investigate the effect of dietary supple-mentation of urea molasses multi-nutrient cake (UMMC) on productive and reproductive performances of rabbit. In Experiment-I, 80 rabbit does (age 24 weeks) were randomly assigned into four dietary treatment groups (T0, T1, T2 and T3) to investigate the effect of UMMC on the reproductive performances whereas in Experiment-II, 112 young rabbits (age 5 weeks) were also randomly assigned into another four dietary treatment groups to investigate the effect of UMMC on the productive performance. The rabbits of four dietary treatment groups (T0, T1, T2 and T3) in both experiments were fed 0%, 4%, 6% and 8% urea containing UMMC, respectively. The results revealed that the dietary supplementation of UMMC increased (P < 0.05) live weight of pregnant does, conception rate, litter size, and litter weight and individual kit weight at weaning. On the other hand, UMMC supplementation reduced (P < 0.05) the kit mortality, though it did not affect the gestation period, litter size, litter weight and individual kit weight at birth. Dietary UMMC supplementation also improved (P < 0.05) the productive performances of growing rabbits in terms of live weight gain, feed conversion ratio and performance index by almost same amount of feed intake. On the contrary, blood parameters (Hb, PCV and ESR) were not affected by the UMMC except for red blood cell and white blood cell counts, but were within their normal range indicating the safe use of UMMC. UMMC also increased (P < 0.05) the fecal bacterial population. Production cost of UMMC was around 0.19 USD less in each kg of feed than the commercial pellet. In both of the experiments rabbits fed 6% urea containing UMMC showed best result than control and other treatment groups as well.
文摘Background: Mammalian ovaries contain follicles containing an oocyte enclosed by layers of granulosa cells (GC). Follicle growth and oocyte maturation are largely dependent on GC numbers and viability, but there is no established, reliable method for assessing the number of viable GC within an isolated follicle. Methods: Centrifugation conditions and the Trypan Blue (TB) Exclusion assay were optimised for low cell densities compatible with the numbers of GC in follicles. Mouse ovarian follicles were disaggregated to produce a single cell suspension of GC which were examined by TB (n = 4), but also by crystal violet assay in a 96-well plate format after 24 h in vitro (n = 3). GC viability in vitro was characterised further by using enzyme-linked immunoassays to quantify GC production of anti-Mullerian hormone (AMH) and estrogen. Results: The centrifugation and low cell density TB protocol could accurately measure the viability of 78 GC in 10 μL, with an intra-assay coefficient of variation (CoV) 22%, and inter-assay CoV 7%. The best follicle disaggregation method (30 min 37°C exposure to 2 mg/mL collagenase prior to 30 min exposure to 0.025% hyaluronidase) yielded (656 ±87) GC per antral follicle of which 82% ±5% were viable. Culturing 312 - 20,000 GC per well for 24 hours and assessing viability by crystal violet assay generated a linear correlation between OD value and viable GC number (R2 = 0.98) and estrogen concentration per well (R2 = 0.92). 20,000 GC per well produced 143 ±16 pg/mL estrogen during 24 hours in vitro, but no detectable AMH. Conclusion: This is the first report describing the isolation of viable, estrogen-producing GC from murine follicles, and their subsequent culture. These procedures are transferrable to other species including humans and can be applied to screening the reproductive toxicity of pharmaceutical agents.
