There are many patients in the blood purification center who need maintenance hemodialysis to maintain life. Those patients generally havelow resistance and are easily exposed to coronavirus because they go back and f...There are many patients in the blood purification center who need maintenance hemodialysis to maintain life. Those patients generally havelow resistance and are easily exposed to coronavirus because they go back and forth the hospital and residence three times a week andclosely contact with family, caregivers, community personnel, people in various means of transportation, medical staff, and other patientsvisiting hospital. Therefore, the blood purification center has become a high‑risk environment for the spread of COVID-19 infection. In viewof this, our center quickly responded to the formulation and implementation of infection prevention and control measures suitable for thecharacteristics of the blood purification center and continuous renal replacement therapy (CRRT) emergency plan for fever and suspectedpatients. According to these measures, we have a positive effect on preventing and controlling nosocomial infection in the blood purificationcenter.展开更多
Land use reflects human activities on land.Urban land use is the highest level human alteration on Earth,and it is rapidly changing due to population increase and urbanization.Urban areas have widespread effects on lo...Land use reflects human activities on land.Urban land use is the highest level human alteration on Earth,and it is rapidly changing due to population increase and urbanization.Urban areas have widespread effects on local hydrology,climate,biodiversity,and food production[1,2].However,maps,that contain knowledge on the distribution,pattern and composition of various land use types in urban areas,are limited to city level.The mapping standard on data sources,methods,land use classification schemes varies from city to city,due to differences in financial input and skills of mapping personnel.To address various national and global environmental challenges caused by urbanization,it is important to have urban land uses at the national and global scales that are derived from the same or consistent data sources with the same or compatible classification systems and mapping methods.This is because,only with urban land use maps produced with similar criteria,consistent environmental policies can be made,and action efforts can be compared and assessed for large scale environmental administration.However,despite of the fact that a number of urban-extent maps exist at global scales[3,4],more detailed urban land use maps do not exist at the same scale.Even at big country or regional levels such as for the United States,China and European Union,consistent land use mapping efforts are rare[5,6](e.g.,https://sdi4apps.eu/open_land_use/).展开更多
Accurately representing and rendering dynamic scenes over time remains a central challenge in neural rendering and computer graphics.Existing dynamic Gaussian-based methods often suffer from limited temporal consisten...Accurately representing and rendering dynamic scenes over time remains a central challenge in neural rendering and computer graphics.Existing dynamic Gaussian-based methods often suffer from limited temporal consistency,flickering under fast motion,and poor adaptability to non-human structures.To address these issues,we propose DG-4DGS,a deformation-graph-constrained 4D Gaussian splatting framework for temporally stable dynamic rendering.The method anchors all Gaussians in a canonical space and enforces cross-frame geometric alignment through a deformation graph.Based on neighborhood-consistency features,a multi-head residual decoder refines position,rotation/scale,and color attributes to achieve fine-detail fidelity without relying on online densification or pruning.Compared with 4DGS and avatar-based approaches,DG-4DGS achieves higher PSNR(peak signal-to-noise ratio)and SSIM(structural similarity index measure)scores and significantly smaller model size on both the TalkBody4D(human)and Horse(non-human)datasets.It effectively suppresses temporal flickering and cross-frame drift in high-frequency regions such as hair strands,cloth wrinkles,and limb extremities.The framework does not depend on parametric templates,facilitating extension to non-human and complex clothing scenarios,though its performance still depends on deformation-tracking quality and neighborhood topology selection.展开更多
The respiro-fermentative metabolism in the yeast Saccharomyces cerevisiae,also called the Crabtree effect,results in lower energy efficiency and biomass yield which can impact yields of chemicals to be produced using ...The respiro-fermentative metabolism in the yeast Saccharomyces cerevisiae,also called the Crabtree effect,results in lower energy efficiency and biomass yield which can impact yields of chemicals to be produced using this cell factory.Although it can be engineered to become Crabtree negative,the slow growth and glucose consumption rate limit its industrial application.Here the Crabtree effect in yeast can be alleviated by engineering the transcription factor Mth1 involved in glucose signaling and a subunit of the RNA polymerase II mediator complex Med2.It was found that the mutant with the MTH1A81D&MED2*432Y allele could grow in glucose rich medium with a specific growth rate of 0.30 h^(-1),an ethanol yield of 0.10 g g^(-1),and a biomass yield of 0.21 g g^(-1),compared with a specific growth rate of 0.40 h^(-1),an ethanol yield of 0.46 g g^(-1),and a biomass yield of 0.11 g g^(-1) in the wild-type strain CEN.PK 113-5D.Transcriptome analysis revealed significant downregulation of the glycolytic process,as well as the upregulation of the TCA cycle and the electron transfer chain.Significant expression changes of several reporter transcription factors were also identified,which might explain the higher energy efficiencies in the engineered strain.We further demonstrated the potential of the engineered strain with the production of 3-hydroxypropionic acid at a titer of 2.04 g L^(-1),i.e.,5.4-fold higher than that of a reference strain,indicating that the alleviated glucose repression could enhance the supply of mitochondrial acetyl-CoA.These results suggested that the engineered strain could be used as an efficient cell factory for mitochondrial production of acetyl-CoA derived chemicals.展开更多
文摘There are many patients in the blood purification center who need maintenance hemodialysis to maintain life. Those patients generally havelow resistance and are easily exposed to coronavirus because they go back and forth the hospital and residence three times a week andclosely contact with family, caregivers, community personnel, people in various means of transportation, medical staff, and other patientsvisiting hospital. Therefore, the blood purification center has become a high‑risk environment for the spread of COVID-19 infection. In viewof this, our center quickly responded to the formulation and implementation of infection prevention and control measures suitable for thecharacteristics of the blood purification center and continuous renal replacement therapy (CRRT) emergency plan for fever and suspectedpatients. According to these measures, we have a positive effect on preventing and controlling nosocomial infection in the blood purificationcenter.
基金partially supported by the National Key Research and Development Program of China(2016YFA0600104)supported by donations made by Delos Living LLC,and the Cyrus Tang Foundation+2 种基金supported by the National Natural Science Foundation of China(41471419)Beijing Institute of Urban Planningsupported by the Fundamental Research Funds for the Central Universities(CCNU19TD002).
文摘Land use reflects human activities on land.Urban land use is the highest level human alteration on Earth,and it is rapidly changing due to population increase and urbanization.Urban areas have widespread effects on local hydrology,climate,biodiversity,and food production[1,2].However,maps,that contain knowledge on the distribution,pattern and composition of various land use types in urban areas,are limited to city level.The mapping standard on data sources,methods,land use classification schemes varies from city to city,due to differences in financial input and skills of mapping personnel.To address various national and global environmental challenges caused by urbanization,it is important to have urban land uses at the national and global scales that are derived from the same or consistent data sources with the same or compatible classification systems and mapping methods.This is because,only with urban land use maps produced with similar criteria,consistent environmental policies can be made,and action efforts can be compared and assessed for large scale environmental administration.However,despite of the fact that a number of urban-extent maps exist at global scales[3,4],more detailed urban land use maps do not exist at the same scale.Even at big country or regional levels such as for the United States,China and European Union,consistent land use mapping efforts are rare[5,6](e.g.,https://sdi4apps.eu/open_land_use/).
文摘Accurately representing and rendering dynamic scenes over time remains a central challenge in neural rendering and computer graphics.Existing dynamic Gaussian-based methods often suffer from limited temporal consistency,flickering under fast motion,and poor adaptability to non-human structures.To address these issues,we propose DG-4DGS,a deformation-graph-constrained 4D Gaussian splatting framework for temporally stable dynamic rendering.The method anchors all Gaussians in a canonical space and enforces cross-frame geometric alignment through a deformation graph.Based on neighborhood-consistency features,a multi-head residual decoder refines position,rotation/scale,and color attributes to achieve fine-detail fidelity without relying on online densification or pruning.Compared with 4DGS and avatar-based approaches,DG-4DGS achieves higher PSNR(peak signal-to-noise ratio)and SSIM(structural similarity index measure)scores and significantly smaller model size on both the TalkBody4D(human)and Horse(non-human)datasets.It effectively suppresses temporal flickering and cross-frame drift in high-frequency regions such as hair strands,cloth wrinkles,and limb extremities.The framework does not depend on parametric templates,facilitating extension to non-human and complex clothing scenarios,though its performance still depends on deformation-tracking quality and neighborhood topology selection.
基金The authors thank for the support from the National Key Research and Development Program of China(2018YFA0900201)National Natural Science Foundation of China(21808008 and 21908004)+2 种基金the Fundamental Research Funds for the Central Universities(buctrc201801)the Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technologythe Knut and Alice Wallenberg Foundation.
文摘The respiro-fermentative metabolism in the yeast Saccharomyces cerevisiae,also called the Crabtree effect,results in lower energy efficiency and biomass yield which can impact yields of chemicals to be produced using this cell factory.Although it can be engineered to become Crabtree negative,the slow growth and glucose consumption rate limit its industrial application.Here the Crabtree effect in yeast can be alleviated by engineering the transcription factor Mth1 involved in glucose signaling and a subunit of the RNA polymerase II mediator complex Med2.It was found that the mutant with the MTH1A81D&MED2*432Y allele could grow in glucose rich medium with a specific growth rate of 0.30 h^(-1),an ethanol yield of 0.10 g g^(-1),and a biomass yield of 0.21 g g^(-1),compared with a specific growth rate of 0.40 h^(-1),an ethanol yield of 0.46 g g^(-1),and a biomass yield of 0.11 g g^(-1) in the wild-type strain CEN.PK 113-5D.Transcriptome analysis revealed significant downregulation of the glycolytic process,as well as the upregulation of the TCA cycle and the electron transfer chain.Significant expression changes of several reporter transcription factors were also identified,which might explain the higher energy efficiencies in the engineered strain.We further demonstrated the potential of the engineered strain with the production of 3-hydroxypropionic acid at a titer of 2.04 g L^(-1),i.e.,5.4-fold higher than that of a reference strain,indicating that the alleviated glucose repression could enhance the supply of mitochondrial acetyl-CoA.These results suggested that the engineered strain could be used as an efficient cell factory for mitochondrial production of acetyl-CoA derived chemicals.
