Hybrid potato breeding based on diploid inbred lines is transforming the way of genetic improvement of this staple food crop,which requires a deep understanding of potato domestication and differentiation.In the prese...Hybrid potato breeding based on diploid inbred lines is transforming the way of genetic improvement of this staple food crop,which requires a deep understanding of potato domestication and differentiation.In the present study,we resequenced 314 diploid wild and landrace accessions to generate a variome map of 47,203,407 variants.Using the variome map,we discovered the reshaping of tuber transcriptome during potato domestication,characterized genome-wide differentiation between landrace groups Stenotomum and Phureja.We identified a jasmonic acid biosynthetic gene possibly affecting the tuber dormancy period.Genome-wide association studies revealed a UDP-glycosyltransferase gene for the biosynthesis of anti-nutritional steroidal glycoalkaloids(SGAs),and a Dehydration Responsive Element Binding(DREB)transcription factor conferring increased average tuber weight.In addition,genome similarity and group-specific SNP analyses indicated that tetraploid potatoes originated from the diploid Solanum tuberosum group Stenotomum.These findings shed light on the evolutionary trajectory of potato domestication and improvement,providing a solid foundation for advancing hybrid potato-breeding practices.展开更多
High heterozygosity and tetrasomic inheritance complicate studies of asexually propagated polyploids,such as potato.Reverse genetics approaches,especially mutant library construction,can be an ideal choice if a proper...High heterozygosity and tetrasomic inheritance complicate studies of asexually propagated polyploids,such as potato.Reverse genetics approaches,especially mutant library construction,can be an ideal choice if a proper mutagenesis genotype is available.Here,we aimed to generate a model system for potato research using anther cultures of Solanum verrucosum,a self-compatible diploid potato with strong late blight resistance.Six of the 23 regenerants obtained(SVA4,SVA7,SVA22,SVA23,SVA32,and SVA33)were diploids,and their homozygosity was estimated to be>99.99%with 22 polymorphic In Del makers.Two lines-SVA4 and SVA32-had reduced stature(plant height≤80 cm),high seed yield(>1,000 seeds/plant),and good tuber set(>30 tubers/plant).We further confirmed the full homozygosity of SVA4 and SVA32 using wholegenome resequencing.These two regenerants possess all the characteristics of a model plant:diploidy,100%homozygosity,self-compatibility,and amenability to transgenesis.Thus,we have successfully generated two lines,SVA4 and SVA32,which can potentially be used for mutagenesis and as model plants to rejuvenate current methods of conducting potato research.展开更多
基金supported by funding from the National Key Research and Development Program of China(2021YFD1201400)the Inner Mongolia Autonomous Region Science and Technology Innovation Guide Award Fund,Special Project for First-Class Discipline Research of Department of Education,Inner Mongolia Autonomous Region(YLXKZX-ND-028)+7 种基金the Central Guidance on Local Science and Technology Development Fund(2022ZY0141)the Program for Innovative Research Team in Universities of Inner Mongolia Autonomous Region(NMGIRT2410)to J.Q.the Agricultural Science and Technology Innovation Program(CAAS-ZDXT201804)and Shenzhen Outstanding Talent Training Fund to S.H.the National Natural Science Foundation of China(32372720)to J.Z.Yunnan Science Fund(202105AF150028)to Y.S.the National Key Research and Development Program of China(2023YFF1000100)to T.L.China Agriculture Research System of MOF and MARA(CARS-09-P17)Key Technology Research Project of Inner Mongolia Autonomous Region(2020GG0054)to R.Z.
文摘Hybrid potato breeding based on diploid inbred lines is transforming the way of genetic improvement of this staple food crop,which requires a deep understanding of potato domestication and differentiation.In the present study,we resequenced 314 diploid wild and landrace accessions to generate a variome map of 47,203,407 variants.Using the variome map,we discovered the reshaping of tuber transcriptome during potato domestication,characterized genome-wide differentiation between landrace groups Stenotomum and Phureja.We identified a jasmonic acid biosynthetic gene possibly affecting the tuber dormancy period.Genome-wide association studies revealed a UDP-glycosyltransferase gene for the biosynthesis of anti-nutritional steroidal glycoalkaloids(SGAs),and a Dehydration Responsive Element Binding(DREB)transcription factor conferring increased average tuber weight.In addition,genome similarity and group-specific SNP analyses indicated that tetraploid potatoes originated from the diploid Solanum tuberosum group Stenotomum.These findings shed light on the evolutionary trajectory of potato domestication and improvement,providing a solid foundation for advancing hybrid potato-breeding practices.
基金supported by the National Natural Science Foundation of China(31660415)Natural Science Foundation of Inner Mongolia Autonomous Region(2016JQ03 and 2016MS0332)+1 种基金Inner Mongolia Autonomous Region Science and Technology Innovation Guide Award Fund to J.Q.Inner Mongolia Young Technological Talents Fund。
文摘High heterozygosity and tetrasomic inheritance complicate studies of asexually propagated polyploids,such as potato.Reverse genetics approaches,especially mutant library construction,can be an ideal choice if a proper mutagenesis genotype is available.Here,we aimed to generate a model system for potato research using anther cultures of Solanum verrucosum,a self-compatible diploid potato with strong late blight resistance.Six of the 23 regenerants obtained(SVA4,SVA7,SVA22,SVA23,SVA32,and SVA33)were diploids,and their homozygosity was estimated to be>99.99%with 22 polymorphic In Del makers.Two lines-SVA4 and SVA32-had reduced stature(plant height≤80 cm),high seed yield(>1,000 seeds/plant),and good tuber set(>30 tubers/plant).We further confirmed the full homozygosity of SVA4 and SVA32 using wholegenome resequencing.These two regenerants possess all the characteristics of a model plant:diploidy,100%homozygosity,self-compatibility,and amenability to transgenesis.Thus,we have successfully generated two lines,SVA4 and SVA32,which can potentially be used for mutagenesis and as model plants to rejuvenate current methods of conducting potato research.
