Background:Burkholderia pseudomallei is a gram-negative bacterium widely found in Southeast Asia and north-ern Australia.This bacterium,which lacks an available vaccine,is the causative agent of melioidosis and has pr...Background:Burkholderia pseudomallei is a gram-negative bacterium widely found in Southeast Asia and north-ern Australia.This bacterium,which lacks an available vaccine,is the causative agent of melioidosis and has properties that potentially enable its exploitation as a bioweapon.Methods:Polymerase chain reaction assays targeting each of the lipopolysaccharide(LPS)genetic types were used to investigate genotype frequencies in B.pseudomallei populations.Silver staining,gas chromatography-mass spectrometry(GC-MS),and immunofluorescence were used to characterize LPS.Results:In our study,a total of 169 clinical B.pseudomallei isolates were collected from Hainan Province,China between 2004 and 2016.The results showed that LPS genotype A was the predominant type,comprising 91.1%of the samples,compared with only 8.9%of LPS genotype B.The majority of patients were male and were diagnosed with sepsis or pneumonia.Silver staining and GC-MS demonstrated that LPS genotypes A and B exhibited distinct phenotypes and molecular structures.Immunofluorescence tests showed there was no cross-reaction between LPS genotypes A and B.Conclusions:This is the first report on the molecular epidemiology of B.pseudomallei based on O-antigen in China.Tracking the regional distribution of different LPS genotypes offers significant insights relevant to the development and administration of LPS-based vaccines.展开更多
基金supported by the National Natural Science Foundation of China(No.81901681 and No.82272350)the Science and Technology Innovation Ability Enhancement Special Project of Army Medical University(No.2019XQN04).
文摘Background:Burkholderia pseudomallei is a gram-negative bacterium widely found in Southeast Asia and north-ern Australia.This bacterium,which lacks an available vaccine,is the causative agent of melioidosis and has properties that potentially enable its exploitation as a bioweapon.Methods:Polymerase chain reaction assays targeting each of the lipopolysaccharide(LPS)genetic types were used to investigate genotype frequencies in B.pseudomallei populations.Silver staining,gas chromatography-mass spectrometry(GC-MS),and immunofluorescence were used to characterize LPS.Results:In our study,a total of 169 clinical B.pseudomallei isolates were collected from Hainan Province,China between 2004 and 2016.The results showed that LPS genotype A was the predominant type,comprising 91.1%of the samples,compared with only 8.9%of LPS genotype B.The majority of patients were male and were diagnosed with sepsis or pneumonia.Silver staining and GC-MS demonstrated that LPS genotypes A and B exhibited distinct phenotypes and molecular structures.Immunofluorescence tests showed there was no cross-reaction between LPS genotypes A and B.Conclusions:This is the first report on the molecular epidemiology of B.pseudomallei based on O-antigen in China.Tracking the regional distribution of different LPS genotypes offers significant insights relevant to the development and administration of LPS-based vaccines.
