Cells are the basic unit of human organs that are not fully understood.The revolutionary advancements of optical imaging alowed us to observe single cells in whole organs,revealing the complicated composition of cells...Cells are the basic unit of human organs that are not fully understood.The revolutionary advancements of optical imaging alowed us to observe single cells in whole organs,revealing the complicated composition of cells with spatial information.Therefore,in this review,we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals.Then,we describe optical imaging to achieve threedimensional spatial discrimination for biological tisutes.Due to the milky appearance of tissues,the spatial information burred deep in the whole organ.Fortunately,strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.展开更多
Reflective dark field microscopy is used to observe the decrease in the light scattered from Ag nanoparticles immobilised on differing solid substrates.The nanoparticles are exposed to solutions containing halide ions...Reflective dark field microscopy is used to observe the decrease in the light scattered from Ag nanoparticles immobilised on differing solid substrates.The nanoparticles are exposed to solutions containing halide ions,both at open circuit and under potentiostatic control,leading to the loss of the nanomaterial.By coupling optical and electrochemical techniques the physical origin of this transformation is demonstrated to be the electrochemical dissolution of the metal nanoparticles driven by electron transfer to ultra-trace dissolved oxygen.The dissolution kinetics of the surface-supported metal nanoparticles is compared on four substrate materials(i.e.,glass,indium titanium oxide,glassy carbon and platinum)with different electrical conductivity.The three conductive substrates catalyse the redox-driven dissolution of Ag nanoparticles with the electrons transferred from the nanoparticles,via the macroscopic electrode to the dioxygen electron acceptor.展开更多
基金supported by the National Science and Technology Innovation 2030 Grant No. (2021ZD0200104)National Nature Science Foundation of China (81871082).
文摘Cells are the basic unit of human organs that are not fully understood.The revolutionary advancements of optical imaging alowed us to observe single cells in whole organs,revealing the complicated composition of cells with spatial information.Therefore,in this review,we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals.Then,we describe optical imaging to achieve threedimensional spatial discrimination for biological tisutes.Due to the milky appearance of tissues,the spatial information burred deep in the whole organ.Fortunately,strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.
文摘Reflective dark field microscopy is used to observe the decrease in the light scattered from Ag nanoparticles immobilised on differing solid substrates.The nanoparticles are exposed to solutions containing halide ions,both at open circuit and under potentiostatic control,leading to the loss of the nanomaterial.By coupling optical and electrochemical techniques the physical origin of this transformation is demonstrated to be the electrochemical dissolution of the metal nanoparticles driven by electron transfer to ultra-trace dissolved oxygen.The dissolution kinetics of the surface-supported metal nanoparticles is compared on four substrate materials(i.e.,glass,indium titanium oxide,glassy carbon and platinum)with different electrical conductivity.The three conductive substrates catalyse the redox-driven dissolution of Ag nanoparticles with the electrons transferred from the nanoparticles,via the macroscopic electrode to the dioxygen electron acceptor.
