[Objectives] To study the anti-inflammatory activity and mechanism of tanshinone I,cryptotanshinone and 15,16-dihydrotanshinone I on HAECs induced by TNF-α. [Methods]Vitamin E was used as a positive control and TNF-...[Objectives] To study the anti-inflammatory activity and mechanism of tanshinone I,cryptotanshinone and 15,16-dihydrotanshinone I on HAECs induced by TNF-α. [Methods]Vitamin E was used as a positive control and TNF-α-induced human aortic endothelial cells( HAECs) were selected as the inflammation model cells. The m RNA levels of IL-6,ICAM-1,VCAM-1,and NF-κB were analyzed by quantitative RT-PCR. The protein expression of NF-κB,ICAM-1,VCAM-1 and phosphorylation of ERK1/2 were determined by Western blot. The adhesion of U937 to HAECs was assessed by BCECF/AM labeling assay. [Results] TNF-α-induced over-expression of IL-6,NF-κB,ICAM-1,and VCAM-1 in HAECs were down-regulated by tanshinone I( TAN),cryptotanshinone( CPT) and 15,16-dihydrotanshinone I( DHT) both in m RNA and protein levels,respectively. Meanwhile 15,16-dihydrotanshinone I and cryptotanshinone could inhibit phosphorylation of ERK1/2 and tanshinone I inhibited U937 adhesion to HAECs significantly. [Conclusions] Tanshinones could inhibit TNF-α induced inflammatory responses on HAECs and the mechanism might be related to inhibition of phosphorylation of ERK1/2 and blocking NF-κB signaling pathway.展开更多
Using the quantum interference of photon pairs in N-stage nonlinear interferometers(NLIs),the contour of the joint spectral function can be modified into an islands pattern.We perform two series of experiments.One is ...Using the quantum interference of photon pairs in N-stage nonlinear interferometers(NLIs),the contour of the joint spectral function can be modified into an islands pattern.We perform two series of experiments.One is that all of the nonlinear fibers in pulse pumped NLIs are identical;the other is that the lengths of N pieces of nonlinear fibers are different.We not only demonstrate how the pattern of spectral function changes with the stage number N,but also characterize how the relative intensity of island peaks varies with N.The results well agree with theoretical predictions,revealing that the NLI with lengths of N pieces of nonlinear fibers following binomial distribution can provide a better active filtering function.Our investigation shows that the active filtering effect of multi-stage NLI is a useful tool for efficiently engineering the factorable two-photon state—a desirable resource for quantum information processing.展开更多
基金Supported by the National Natural Science Foundation of China(81274031)
文摘[Objectives] To study the anti-inflammatory activity and mechanism of tanshinone I,cryptotanshinone and 15,16-dihydrotanshinone I on HAECs induced by TNF-α. [Methods]Vitamin E was used as a positive control and TNF-α-induced human aortic endothelial cells( HAECs) were selected as the inflammation model cells. The m RNA levels of IL-6,ICAM-1,VCAM-1,and NF-κB were analyzed by quantitative RT-PCR. The protein expression of NF-κB,ICAM-1,VCAM-1 and phosphorylation of ERK1/2 were determined by Western blot. The adhesion of U937 to HAECs was assessed by BCECF/AM labeling assay. [Results] TNF-α-induced over-expression of IL-6,NF-κB,ICAM-1,and VCAM-1 in HAECs were down-regulated by tanshinone I( TAN),cryptotanshinone( CPT) and 15,16-dihydrotanshinone I( DHT) both in m RNA and protein levels,respectively. Meanwhile 15,16-dihydrotanshinone I and cryptotanshinone could inhibit phosphorylation of ERK1/2 and tanshinone I inhibited U937 adhesion to HAECs significantly. [Conclusions] Tanshinones could inhibit TNF-α induced inflammatory responses on HAECs and the mechanism might be related to inhibition of phosphorylation of ERK1/2 and blocking NF-κB signaling pathway.
基金the National Key Research and Development Program of China(No.2016YFA0301403)the National Natural Science Foundation of China(Nos.11527808 and 11874279)the Science and Technology Program ofTianjin(No.18ZXZNGX00210)。
文摘Using the quantum interference of photon pairs in N-stage nonlinear interferometers(NLIs),the contour of the joint spectral function can be modified into an islands pattern.We perform two series of experiments.One is that all of the nonlinear fibers in pulse pumped NLIs are identical;the other is that the lengths of N pieces of nonlinear fibers are different.We not only demonstrate how the pattern of spectral function changes with the stage number N,but also characterize how the relative intensity of island peaks varies with N.The results well agree with theoretical predictions,revealing that the NLI with lengths of N pieces of nonlinear fibers following binomial distribution can provide a better active filtering function.Our investigation shows that the active filtering effect of multi-stage NLI is a useful tool for efficiently engineering the factorable two-photon state—a desirable resource for quantum information processing.
