Phytosterols(PS)are natural steroidal compounds with multiple physiological functions,but their poor solubi-lity,high crystallinity,and low bioavailability hinder effective utilization.In this study,PS-loaded nano-str...Phytosterols(PS)are natural steroidal compounds with multiple physiological functions,but their poor solubi-lity,high crystallinity,and low bioavailability hinder effective utilization.In this study,PS-loaded nano-structured lipid carriers modified with cross-linked chitosan(CLCS-PS-NLCs)developed via emulsificationevaporation combined with high-pressure homogenization.The optimal formulation was achieved with 0.5%(w/w)chitosan and a tripolyphosphate(TPP):chitosan(CS)mass ratio of 2:1 to enhance carrier stability and PS absorption.Structural characterization using dynamic light scattering(DLS)and transmission electron micro-scopy(TEM),along with fourier transform infrared(FTIR),X-ray diffraction(XRD),and differential scanning calorimetry(DSC),confirmed efficient PS encapsulation and successful chitosan modification.CLCS-PS-NLCs demonstrated excellent stability under varying pH,ionic strength,and storage conditions.In vitro and in vivo studies showed improved digestion and absorption of PS,achieving a bioaccessibility of 36.65%compared to only 13.28%for PS-NLCs,and enhancing its uptake in rats.This study provides a promising delivery system for PS with potential applications in functional foods and pharmaceuticals.展开更多
Lunasin,a novel bioactive peptide initially found in soybean,exhibits multiple biological functions.Here,we overexpressed lunasin in maize to generated the transgenic maize with high lunasin content for application in...Lunasin,a novel bioactive peptide initially found in soybean,exhibits multiple biological functions.Here,we overexpressed lunasin in maize to generated the transgenic maize with high lunasin content for application in functional food.A maize variety YF464 was screened and used as receptor material(WT),and the identification of lunasin peptide in maize was demonstrated through Western blot and ultrahigh pressure liquid chromatography-electrospray ionization with tandem mass spectrometry(UPLC-ESI-MS/MS).Thirteen overexpression(OE)lines were obtained,then OE1(182.34±13.62μg g^(-1))and OE2(294.51±34.25μg g^(-1))were selected for further study based on high expression of lunasin.Lunasin extract from maize(LEM)presented significantly higher antioxidant,anti-inflammatory,and anti-cancer activity in OE lines than that from WT.Furthermore,LEM from OE1 and OE2 showed superior anti-proliferation function on human breast cancer cells MDA-MB-231 than that from WT.Combination analysis with matched proteins from transcriptome and proteome after lunasin exposure showed that genes and proteins associated with mitophagy was highly activated in MDA-MB-231 cells,indicated lunasin may act an important role in promoting mitophagy,inducing cell death.Therefore,lunasin overexpressing maize has a great potential as a functional food in the diets of patients with cancer,together with an alternative for dietary nutrition and health care in the future.More importantly,it will provide the scientific data for further exploration on its anti-cancer function.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32272302).
文摘Phytosterols(PS)are natural steroidal compounds with multiple physiological functions,but their poor solubi-lity,high crystallinity,and low bioavailability hinder effective utilization.In this study,PS-loaded nano-structured lipid carriers modified with cross-linked chitosan(CLCS-PS-NLCs)developed via emulsificationevaporation combined with high-pressure homogenization.The optimal formulation was achieved with 0.5%(w/w)chitosan and a tripolyphosphate(TPP):chitosan(CS)mass ratio of 2:1 to enhance carrier stability and PS absorption.Structural characterization using dynamic light scattering(DLS)and transmission electron micro-scopy(TEM),along with fourier transform infrared(FTIR),X-ray diffraction(XRD),and differential scanning calorimetry(DSC),confirmed efficient PS encapsulation and successful chitosan modification.CLCS-PS-NLCs demonstrated excellent stability under varying pH,ionic strength,and storage conditions.In vitro and in vivo studies showed improved digestion and absorption of PS,achieving a bioaccessibility of 36.65%compared to only 13.28%for PS-NLCs,and enhancing its uptake in rats.This study provides a promising delivery system for PS with potential applications in functional foods and pharmaceuticals.
基金financially supported by Key Research and Development Program of Shanxi Province(2022ZDYF108-02)Shanxi Postdoctoral Reward Funds for Research Projects(SXBYKY2023005)+2 种基金Shanxi Fundamental Research Program for Youths(202203021222153)Young Scientists Fund of the National Natural Science Foundation of China(32302362)Senior Foreign Experts introducing Project(G202204011L).
文摘Lunasin,a novel bioactive peptide initially found in soybean,exhibits multiple biological functions.Here,we overexpressed lunasin in maize to generated the transgenic maize with high lunasin content for application in functional food.A maize variety YF464 was screened and used as receptor material(WT),and the identification of lunasin peptide in maize was demonstrated through Western blot and ultrahigh pressure liquid chromatography-electrospray ionization with tandem mass spectrometry(UPLC-ESI-MS/MS).Thirteen overexpression(OE)lines were obtained,then OE1(182.34±13.62μg g^(-1))and OE2(294.51±34.25μg g^(-1))were selected for further study based on high expression of lunasin.Lunasin extract from maize(LEM)presented significantly higher antioxidant,anti-inflammatory,and anti-cancer activity in OE lines than that from WT.Furthermore,LEM from OE1 and OE2 showed superior anti-proliferation function on human breast cancer cells MDA-MB-231 than that from WT.Combination analysis with matched proteins from transcriptome and proteome after lunasin exposure showed that genes and proteins associated with mitophagy was highly activated in MDA-MB-231 cells,indicated lunasin may act an important role in promoting mitophagy,inducing cell death.Therefore,lunasin overexpressing maize has a great potential as a functional food in the diets of patients with cancer,together with an alternative for dietary nutrition and health care in the future.More importantly,it will provide the scientific data for further exploration on its anti-cancer function.
