Objective: This study aims to examine the causal relationship between inflammatory factors and the probability of developing vascular dementia (VD) using Mendelian Randomization (MR) and Chinese herbal medicine predic...Objective: This study aims to examine the causal relationship between inflammatory factors and the probability of developing vascular dementia (VD) using Mendelian Randomization (MR) and Chinese herbal medicine prediction method, and to screen potential Chinese herbal medicines for the prevention and treatment of VD. Methods: Single nucleotide polymorphisms (SNPs) that exhibit a strong association with vascular dementia (VD) were identified as instrumental variables from the summary statistics of genome-wide association studies (GWAS). The primary analytical method employed was inverse variance weighting (IVW), while auxiliary analyses included the MR-Egger method, weighted median method, simple model, and weighted model. A two-way Mendelian randomization analysis was conducted to assess the causal relationship between inflammatory factors and the risk of VD, thereby identifying the key inflammatory factors involved. The MR-Egger intercept test and Cochran’s Q test were employed to assess the horizontal polymorphism and heterogeneity of instrumental variables. A sensitivity analysis was conducted by excluding one method at a time. Ultimately, based on key inflammatory factors, predictions for the prevention and treatment using traditional Chinese medicine were made, along with the screening of homologous herbal remedies. Results: Based on the results of the forward MR, the probability of developing VD was elevated when the inflammatory factors CXCL10 and CXCL5 were expressed at higher levels, whereas the probability of developing VD decreased as the expression levels of IL-13 and IL-20RA increased. These findings were supported by the assessment of pleiotropy, heterogeneity, and sensitivity. The results of the reverse MR analysis showed that there was no causal relationship between VD, as an exposure dataset, and these four inflammatory factors. According to the key inflammatory factors, 37 Chinese herbal medicines such as Siraitia grosvenorii were selected. Their characteristics including four natures, five flavors, channel tropism and treatment efficiency were cold, warm, neutral, pungent, sweet, bitter, lung meridian, spleen meridian, liver meridian, kidney meridian and clearing heat. Among them, Siraitia grosvenorii, Poria with hostwood, Perilla frutescens, and Radix Platycodi were all medicine and food homologous Chinese herbal medicines. Conclusions: The increase of CXCL10 and CXCL5 expression levels can increase the risk of VD, and the increase of IL-13 and IL-20 RA expression levels can reduce the risk of VD. Siraitia grosvenorii and other Chinese herbal medicines might be potential sources of therapeutic drugs for the treatment of VD. Medicine and food homologous Chinese herbal medicines, such as Siraitia grosvenorii, Poria with hostwood, Perilla frutescens, and Radix Platycodi, may help the elderly population with corresponding Traditional Chinese Medicine (TCM) constitutions to prevent VD.展开更多
Background and Objectives:The precise impact of tea consumption on the risk of depression remains unclear.This study aimed to explore the relationship between the consumption patterns of tea and the likelihood of de p...Background and Objectives:The precise impact of tea consumption on the risk of depression remains unclear.This study aimed to explore the relationship between the consumption patterns of tea and the likelihood of de pression onset,utilizing a two-sample Mendelian randomization(MR)methodology.Methods and S tudy Design:We utilized available genome-wide association study(GWAS)datasets on tea intake and depressive disorders.To investigate the causal relationship between tea consumption and depression,we employed a set of two-sample Mendelian Randomization(MR)methods.These included the inverse-variance weighted(IVW)analysis,weighted median approach,and MR-Egger regression.Additionally,we utilized MR-PRESSO and the MR-Egger intercept test for the detection of pleiotropic effects.To ensure the robustness and consistency of our findings,a sensitivity analysis was carried out,applying the'leave-one-out'strategy.The Bayesian weighted Mendelian ran domization(BWMR)was employed to conduct additional testing on the obtained results.Results:The study's outcomes revealed a causal association between increased tea intake and an increased risk of depression(Inverse-Variance Weighted Analysis:Odds Ratio[OR]=1.029,95%Confidence Interval[CI]:1.003-1.055,p=0.027).This was observed despite variations in instrumental variables and the nonexistence of horizontal pleiotropy.Fur ther more,the robustness of our Mendelian Randomization investigation was affirmed through the implementation of the'leave-one-out'method in our sensitivity analysis.The findings from BWMR were in line with those ob tained from IVW(BWMR:OR=1.030,95%CI:1.003-1.057,p=0.029).Conclusions:The results from this study indicate a substantial and positive causal link between the regularity of tea drinking and the risk of depres sion onset.展开更多
文摘Objective: This study aims to examine the causal relationship between inflammatory factors and the probability of developing vascular dementia (VD) using Mendelian Randomization (MR) and Chinese herbal medicine prediction method, and to screen potential Chinese herbal medicines for the prevention and treatment of VD. Methods: Single nucleotide polymorphisms (SNPs) that exhibit a strong association with vascular dementia (VD) were identified as instrumental variables from the summary statistics of genome-wide association studies (GWAS). The primary analytical method employed was inverse variance weighting (IVW), while auxiliary analyses included the MR-Egger method, weighted median method, simple model, and weighted model. A two-way Mendelian randomization analysis was conducted to assess the causal relationship between inflammatory factors and the risk of VD, thereby identifying the key inflammatory factors involved. The MR-Egger intercept test and Cochran’s Q test were employed to assess the horizontal polymorphism and heterogeneity of instrumental variables. A sensitivity analysis was conducted by excluding one method at a time. Ultimately, based on key inflammatory factors, predictions for the prevention and treatment using traditional Chinese medicine were made, along with the screening of homologous herbal remedies. Results: Based on the results of the forward MR, the probability of developing VD was elevated when the inflammatory factors CXCL10 and CXCL5 were expressed at higher levels, whereas the probability of developing VD decreased as the expression levels of IL-13 and IL-20RA increased. These findings were supported by the assessment of pleiotropy, heterogeneity, and sensitivity. The results of the reverse MR analysis showed that there was no causal relationship between VD, as an exposure dataset, and these four inflammatory factors. According to the key inflammatory factors, 37 Chinese herbal medicines such as Siraitia grosvenorii were selected. Their characteristics including four natures, five flavors, channel tropism and treatment efficiency were cold, warm, neutral, pungent, sweet, bitter, lung meridian, spleen meridian, liver meridian, kidney meridian and clearing heat. Among them, Siraitia grosvenorii, Poria with hostwood, Perilla frutescens, and Radix Platycodi were all medicine and food homologous Chinese herbal medicines. Conclusions: The increase of CXCL10 and CXCL5 expression levels can increase the risk of VD, and the increase of IL-13 and IL-20 RA expression levels can reduce the risk of VD. Siraitia grosvenorii and other Chinese herbal medicines might be potential sources of therapeutic drugs for the treatment of VD. Medicine and food homologous Chinese herbal medicines, such as Siraitia grosvenorii, Poria with hostwood, Perilla frutescens, and Radix Platycodi, may help the elderly population with corresponding Traditional Chinese Medicine (TCM) constitutions to prevent VD.
基金supported by Guangxi University of Chinese Medicine"Qihuang Project"high-level talent team(202410)Academic Team Building Project of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine(146[2018])+2 种基金Guangxi Key Discipline Construction Project of Tradi tional Chinese Medicine(GZXK-Z-20-13)Guangxi Clinical Research Center for Encephalopathy of Traditional Chinese Medicine,No.Guike AD20238028High-level Innovation Team and Outstanding Scholars Program of Universities in Guangxi(Guangxi Education Talents(2020)No.6).
文摘Background and Objectives:The precise impact of tea consumption on the risk of depression remains unclear.This study aimed to explore the relationship between the consumption patterns of tea and the likelihood of de pression onset,utilizing a two-sample Mendelian randomization(MR)methodology.Methods and S tudy Design:We utilized available genome-wide association study(GWAS)datasets on tea intake and depressive disorders.To investigate the causal relationship between tea consumption and depression,we employed a set of two-sample Mendelian Randomization(MR)methods.These included the inverse-variance weighted(IVW)analysis,weighted median approach,and MR-Egger regression.Additionally,we utilized MR-PRESSO and the MR-Egger intercept test for the detection of pleiotropic effects.To ensure the robustness and consistency of our findings,a sensitivity analysis was carried out,applying the'leave-one-out'strategy.The Bayesian weighted Mendelian ran domization(BWMR)was employed to conduct additional testing on the obtained results.Results:The study's outcomes revealed a causal association between increased tea intake and an increased risk of depression(Inverse-Variance Weighted Analysis:Odds Ratio[OR]=1.029,95%Confidence Interval[CI]:1.003-1.055,p=0.027).This was observed despite variations in instrumental variables and the nonexistence of horizontal pleiotropy.Fur ther more,the robustness of our Mendelian Randomization investigation was affirmed through the implementation of the'leave-one-out'method in our sensitivity analysis.The findings from BWMR were in line with those ob tained from IVW(BWMR:OR=1.030,95%CI:1.003-1.057,p=0.029).Conclusions:The results from this study indicate a substantial and positive causal link between the regularity of tea drinking and the risk of depres sion onset.
