Dental caries is one of the most prevalent and costly biofilm-induced oral diseases that causes the deterioration of the mineralized tooth tissue.Traditional antimicro-bial agents like antibiotics and antimicrobial pep...Dental caries is one of the most prevalent and costly biofilm-induced oral diseases that causes the deterioration of the mineralized tooth tissue.Traditional antimicro-bial agents like antibiotics and antimicrobial peptides(AMPs)struggle to effectively eradicate bacteria in biofilms without eliciting resistance.Herein,we demonstrate the construction of FeOOH@Fe-Lysine@Au nanostructured AMPs(nAMPs)dis-tinguished by their AMP-like antibacterial activity and self-producing reactive oxygen species(ROS)capacity for caries treatment.On the one hand,FeOOH@Fe-Lysine@Au nAMPs can catalyze glucose oxidation to generate ROS within the cariogenic biofilm microenvironment,resulting in the disintegration of the extra-cellular polymeric substance matrix and the exposure of bacteria.On the other hand,FeOOH@Fe-Lysine@Au nAMPs can attach to bacterial surfaces via electrostatic attractions,proceeding to damage membranes,disrupt metabolic pathways,and inhibit protein synthesis through the aggregated lysine and the generated ROS.Based on this antibacterial mechanism,FeOOH@Fe-Lysine@Au nAMPs can effectively eradicate Streptococcus mutans and its associated biofilm,significantly impeding the progression of dental caries.Given the straightforward and cost-efficient prepa-ration of FeOOH@Fe-Lysine@Au nAMPs compared to AMPs that require specific sequences,and their minimal adverse impacts on gingival/palatal tissues,major organs,and oral/gut microbiomes,our research may promote the development of novel therapeutic agents in dental health maintenance.展开更多
Background and Objectives:To investigate the capacity of clinical nutrition services in secondary and tertiary hospitals in the Sichuan Province,China.Methods and Study Design:Convenience sampling was used.Equestionna...Background and Objectives:To investigate the capacity of clinical nutrition services in secondary and tertiary hospitals in the Sichuan Province,China.Methods and Study Design:Convenience sampling was used.Equestionnaires were distributed to all eligible medical institutions in Sichuan through the official network of provincial and municipal clinical nutrition quality control centers.The data obtained were sorted in Microsoft Excel and analyzed by SPSS.Results:A total of 519 questionnaires were returned,of which 455 were valid.Only 228 hospitals were accessible to clinical nutrition services,of which 127 hospitals had independently set up clinical nutrition departments(CNDs).The ratio of clinical nutritionists to beds was 1:214.During the last decade,the rate of constructing new CNDs was maintained at approximately 5 units/year.A total of 72.4%of hospitals managed their clinical nutrition units as part of their medical technology departments.The specialist number ratio of senior,associate,intermediate and junior is approximately 1:4:8:10.There were 5 common charges for clinical nutrition.Conclusions:The sample representation was limited,and the capacity of clinical nutrition services may have been overestimated.Secondary and tertiary hospitals in Sichuan are currently in the second high tide of department establishment,with a positive trend of departmental affiliation standardization and a basic formation of a talent echelon.展开更多
基金Science and Technology Development Program of Jilin Province,Grant/Award Numbers:***202302001,20210203090SFScience and Technology Development Program of Changchun City,Grant/Award Number:23***13Special Project from Ministry of Science and Technology of China。
文摘Dental caries is one of the most prevalent and costly biofilm-induced oral diseases that causes the deterioration of the mineralized tooth tissue.Traditional antimicro-bial agents like antibiotics and antimicrobial peptides(AMPs)struggle to effectively eradicate bacteria in biofilms without eliciting resistance.Herein,we demonstrate the construction of FeOOH@Fe-Lysine@Au nanostructured AMPs(nAMPs)dis-tinguished by their AMP-like antibacterial activity and self-producing reactive oxygen species(ROS)capacity for caries treatment.On the one hand,FeOOH@Fe-Lysine@Au nAMPs can catalyze glucose oxidation to generate ROS within the cariogenic biofilm microenvironment,resulting in the disintegration of the extra-cellular polymeric substance matrix and the exposure of bacteria.On the other hand,FeOOH@Fe-Lysine@Au nAMPs can attach to bacterial surfaces via electrostatic attractions,proceeding to damage membranes,disrupt metabolic pathways,and inhibit protein synthesis through the aggregated lysine and the generated ROS.Based on this antibacterial mechanism,FeOOH@Fe-Lysine@Au nAMPs can effectively eradicate Streptococcus mutans and its associated biofilm,significantly impeding the progression of dental caries.Given the straightforward and cost-efficient prepa-ration of FeOOH@Fe-Lysine@Au nAMPs compared to AMPs that require specific sequences,and their minimal adverse impacts on gingival/palatal tissues,major organs,and oral/gut microbiomes,our research may promote the development of novel therapeutic agents in dental health maintenance.
基金supported by the Operating expenses of quality control center from Health Commission of Sichuan Province(TJCZ2016003)Key R&D Projects of Sichuan Science and Technology Bureau,the Construction and Transformation of Comprehensive Evaluation Index system for rational use of Food for Special Medical Purposes in Medical institutions(2021YFS0177).
文摘Background and Objectives:To investigate the capacity of clinical nutrition services in secondary and tertiary hospitals in the Sichuan Province,China.Methods and Study Design:Convenience sampling was used.Equestionnaires were distributed to all eligible medical institutions in Sichuan through the official network of provincial and municipal clinical nutrition quality control centers.The data obtained were sorted in Microsoft Excel and analyzed by SPSS.Results:A total of 519 questionnaires were returned,of which 455 were valid.Only 228 hospitals were accessible to clinical nutrition services,of which 127 hospitals had independently set up clinical nutrition departments(CNDs).The ratio of clinical nutritionists to beds was 1:214.During the last decade,the rate of constructing new CNDs was maintained at approximately 5 units/year.A total of 72.4%of hospitals managed their clinical nutrition units as part of their medical technology departments.The specialist number ratio of senior,associate,intermediate and junior is approximately 1:4:8:10.There were 5 common charges for clinical nutrition.Conclusions:The sample representation was limited,and the capacity of clinical nutrition services may have been overestimated.Secondary and tertiary hospitals in Sichuan are currently in the second high tide of department establishment,with a positive trend of departmental affiliation standardization and a basic formation of a talent echelon.
