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猪瘟病毒E2蛋白BC抗原域的酿酒酵母表面展示 被引量:1
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作者 刘小凤 汪倩 +1 位作者 罗明阳 孙金福 《微生物前沿》 2017年第3期72-78,共7页
为构建猪瘟病毒(CSFV)E2蛋白的B、C抗原域(E2BC)酿酒酵母表面展示体系,以pVEXE2为模板,用PCR克隆了E2BC基因,并插入酵母展示表达载体p1v5AG的BamH I和EcoRI位点,构建重组酵母展示表达载体,用LiAC方法转化酿酒酵母细胞W303,构建重组酵母... 为构建猪瘟病毒(CSFV)E2蛋白的B、C抗原域(E2BC)酿酒酵母表面展示体系,以pVEXE2为模板,用PCR克隆了E2BC基因,并插入酵母展示表达载体p1v5AG的BamH I和EcoRI位点,构建重组酵母展示表达载体,用LiAC方法转化酿酒酵母细胞W303,构建重组酵母菌。重组菌经培养后,对酵母菌细胞进行针对His标签的间接免疫荧光染色,检测His-E2BC融合表达蛋白。结果显示,重组酵母菌表面有绿色荧光,表明E2BC蛋白成功表达于酵母表面,为开发酵母载体口服疫苗奠定了基础。 展开更多
关键词 酵母表面展示 猪瘟病毒 E2BC抗原域 口服疫苗
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Research on the Joint Development of Cities of Guangdong-Hong Kong-Macao Greater Bay Area: A Case Study of Foshan City
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作者 mingyang luo 《Proceedings of Business and Economic Studies》 2021年第5期1-7,共7页
Promoting the construction of Guangdong-Hong Kong-Macao Greater Bay Area is a major policy decision and national strategy made by the central government of China,which significantly supports the Belt and Road Initiati... Promoting the construction of Guangdong-Hong Kong-Macao Greater Bay Area is a major policy decision and national strategy made by the central government of China,which significantly supports the Belt and Road Initiative and maintains the long-term prosperity for the whole country.As an important part of the Guangdong-Hong Kong-Macao Greater Bay Area,Foshan needs to find its own position and direction in order to accelerate the joint development with other cities through five measures. 展开更多
关键词 Guangdong-Hong Kong-Macao Greater Bay Area Joint development City integration Sanlong Bay innovation cluster Manufacturing industry innovation
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