Objectives:Chinese medicine doctors have practiced in the United States since April of 1854,the world's first Chinese medicine doctor's memorial is Kam Wah Chung Museum in John Day,Oregon,U.S.,to commemorate D...Objectives:Chinese medicine doctors have practiced in the United States since April of 1854,the world's first Chinese medicine doctor's memorial is Kam Wah Chung Museum in John Day,Oregon,U.S.,to commemorate Dr.Ing Hay(“Doc Hay”伍于念)and his business partner Lung On(梁光荣).There were few studies to explore Doc Hay's real practice and prescription,while most of papers focused on Doc Hay's legend life.This paper aims to analyze and interpret one of the intriguing herbal prescriptions that Doc Hay had hand-written.Materials and Methods:Dr.Arthur Yin Fan made an academic travel to Kam Wah Chung museum on August 5,2018 and examined the books and hand-written prescriptions of Doc Hay,which were not exhibited to the public at that time.This paper analyzes and interprets one of herbal prescriptions that Doc Hay had hand-written in 1907.Interpretation process included four steps:(1):Transform Doc Hay's hand-written prescription(with original herb names)to standard herb names in Chinese.(2):Induct and rearrange the herb name into groups based on herb property characteristics and then translate them into both PinYin names and English names.Analyzation of the prescription may include in what classic formula(s)routinely introduced in Chinese medicine textbooks.(3):Analyze the herbal action for each group and potential symptoms or conditions the patient may have had.An analysis of classic formula(s)used in the prescription may represent what clinical condition was being treated at that time.(4):Combine the analysis to give a comprehensive picture of the patient.Results:The prescription consists of 67 herbs,total 934.6 grams,and including Yin Qiao Powder(银翘散),Sang Ju Decoction(桑菊饮),Zhi Sou Powder(止嗽散),Qing Ying Decoction(清营汤),Xi Jiao Di Huang Decoction(犀角地黄汤),Long Dan Xie Gan Decoction(龙胆泻肝汤)and Chai Hu Shu Gan Powder(柴胡疏肝散).Speculation indicated that the prescription was for a patient who had obvious pulmonary infection accompanied by severe cough,and probably had prolonged pulmonary tuberculosis(TB)also with acute respiratory infection caused by other bacteria or viruses.Based on the analysis of composition of this prescription,it can be used for the treatment of epidemic diseases.Conclusion:The majority of herbs used in this prescription are commonly included in the herbal medicines in China used to effectively counter severe acute respiratory syndrome(SARS),H1N1 and currently COVID-19,it might be Doc Hay's basic formula for patients with“Spanish Flu”during the 1918-1920 pandemic.展开更多
We report brain imaging and genetic diagnosis in a family from Wuhan, China, with a history of Huntington＇s disease. Among 17 family members across three generations, four patients （Ⅱ2, Ⅱ6, Ⅲ5, and Ⅲ9） show typ...We report brain imaging and genetic diagnosis in a family from Wuhan, China, with a history of Huntington＇s disease. Among 17 family members across three generations, four patients （Ⅱ2, Ⅱ6, Ⅲ5, and Ⅲ9） show typical Huntington＇s disease, involuntary dance-like movements. Magnetic resonance imaging found lateral ventricular atrophy in three members （Ⅱ2, Ⅱ6, and Ⅲ5）. Moreover, genetic analysis identified abnormally amplified CAG sequence repeats （〉 40） in two members （Ⅲ5 and Ⅲ9）. Among borderline cases, with clinical symptoms and brain imaging features of Huntington＇s disease, two cases were identified （Ⅱ2 and Ⅱ6）, but shown by mutation analysis for CAG expansions in the important transcript 15 gene, to be non-Huntington＇s disease. Our findings suggest that clinical diagnosis of Huntington＇s disease requires a combination of clinical symptoms, radiological changes, and genetic diagnosis.展开更多
Sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19.Here,we report a simple yet efficient platform integrating a portable and low-cost custom-made detector and a no...Sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19.Here,we report a simple yet efficient platform integrating a portable and low-cost custom-made detector and a novel microwell array biochip for rapid and accurate detection of SARS-CoV-2.The instrument exhibits expedited amplification speed that enables colorimetric read-out within 25 minutes.A polymeric chip with a laser-engraved microwell array was developed to process the reaction between the primers and the respiratory swab RNA extracts,based on reverse transcriptase loop-mediated isothermal amplification(RT-LAMP).To achieve clinically acceptable performance,we synthesized a group of six primers to identify the conserved regions of the ORF1ab gene of SARS-CoV-2.Clinical trials were conducted with 87 PCR-positive and 43 PCRnegative patient samples.The platform demonstrated both high sensitivity(95.40%)and high specificity(95.35%),showing potentials for rapid and user-friendly diagnosis of COVID-19 among many other infectious pathogens.展开更多
Background:The coronavirus disease 2019(COVID-19)has affected approximately 2 million individuals worldwide;however,data regarding fatal cases have been limited.Objective:To report the clinical features of 162 fatal c...Background:The coronavirus disease 2019(COVID-19)has affected approximately 2 million individuals worldwide;however,data regarding fatal cases have been limited.Objective:To report the clinical features of 162 fatal cases of COVID-19 from 5 hospitals in Wuhan between December 30,2019 and March 12,2020.Methods:The demographic data,signs and symptoms,clinical course,comorbidities,laboratory findings,computed tomographic(CT)scans,treatments,and complications of the patients with fatal cases were retrieved from electronic medical records.Results:The median patient age was 69.5(interquartile range:63.0–77.25)years,and 80%of the patients were over 61 years.A total of 112(69.1%)patients were men.Hypertension(45.1%)was the most common comorbidity,while 59(36.4%)patients had no comorbidity.At admission,131(81.9%)patients had severe or critical COVID-19,whereas 39(18.1%)patients with hypertension or chronic lung disease had moderate COVID-19.In total,126(77.8%)patients received antiviral treatment,while 132(81.5%)patients received glucocorticoid treatment.A total of 116(71.6%)patients were admitted to the intensive care unit(ICU),and 137(85.1%)patients received mechanical ventilation.Most patients received mechanical ventilation before ICU admission.Approximately 93.2%of the patients developed respiratory failure or acute respiratory distress syndrome.There were no significant differences in the inhospital survival time among the hospitals(P=0.14).Conclusion:Young patients with moderate COVID-19 without comorbidity at admission could also develop fatal outcomes.The in-hospital survival time of the fatal cases was similar among the hospitals of different levels in Wuhan.展开更多
文摘Objectives:Chinese medicine doctors have practiced in the United States since April of 1854,the world's first Chinese medicine doctor's memorial is Kam Wah Chung Museum in John Day,Oregon,U.S.,to commemorate Dr.Ing Hay(“Doc Hay”伍于念)and his business partner Lung On(梁光荣).There were few studies to explore Doc Hay's real practice and prescription,while most of papers focused on Doc Hay's legend life.This paper aims to analyze and interpret one of the intriguing herbal prescriptions that Doc Hay had hand-written.Materials and Methods:Dr.Arthur Yin Fan made an academic travel to Kam Wah Chung museum on August 5,2018 and examined the books and hand-written prescriptions of Doc Hay,which were not exhibited to the public at that time.This paper analyzes and interprets one of herbal prescriptions that Doc Hay had hand-written in 1907.Interpretation process included four steps:(1):Transform Doc Hay's hand-written prescription(with original herb names)to standard herb names in Chinese.(2):Induct and rearrange the herb name into groups based on herb property characteristics and then translate them into both PinYin names and English names.Analyzation of the prescription may include in what classic formula(s)routinely introduced in Chinese medicine textbooks.(3):Analyze the herbal action for each group and potential symptoms or conditions the patient may have had.An analysis of classic formula(s)used in the prescription may represent what clinical condition was being treated at that time.(4):Combine the analysis to give a comprehensive picture of the patient.Results:The prescription consists of 67 herbs,total 934.6 grams,and including Yin Qiao Powder(银翘散),Sang Ju Decoction(桑菊饮),Zhi Sou Powder(止嗽散),Qing Ying Decoction(清营汤),Xi Jiao Di Huang Decoction(犀角地黄汤),Long Dan Xie Gan Decoction(龙胆泻肝汤)and Chai Hu Shu Gan Powder(柴胡疏肝散).Speculation indicated that the prescription was for a patient who had obvious pulmonary infection accompanied by severe cough,and probably had prolonged pulmonary tuberculosis(TB)also with acute respiratory infection caused by other bacteria or viruses.Based on the analysis of composition of this prescription,it can be used for the treatment of epidemic diseases.Conclusion:The majority of herbs used in this prescription are commonly included in the herbal medicines in China used to effectively counter severe acute respiratory syndrome(SARS),H1N1 and currently COVID-19,it might be Doc Hay's basic formula for patients with“Spanish Flu”during the 1918-1920 pandemic.
基金supported by the Fundamental Research Funds for the Central Universities,No.20100141110017,20103030201000217 and 201130302020008
文摘We report brain imaging and genetic diagnosis in a family from Wuhan, China, with a history of Huntington＇s disease. Among 17 family members across three generations, four patients （Ⅱ2, Ⅱ6, Ⅲ5, and Ⅲ9） show typical Huntington＇s disease, involuntary dance-like movements. Magnetic resonance imaging found lateral ventricular atrophy in three members （Ⅱ2, Ⅱ6, and Ⅲ5）. Moreover, genetic analysis identified abnormally amplified CAG sequence repeats （〉 40） in two members （Ⅲ5 and Ⅲ9）. Among borderline cases, with clinical symptoms and brain imaging features of Huntington＇s disease, two cases were identified （Ⅱ2 and Ⅱ6）, but shown by mutation analysis for CAG expansions in the important transcript 15 gene, to be non-Huntington＇s disease. Our findings suggest that clinical diagnosis of Huntington＇s disease requires a combination of clinical symptoms, radiological changes, and genetic diagnosis.
基金supported by the Special Research Funding on COVID19 from Sichuan Province of China(Grant No.2020YFS0004)Science and Technology Department,Ministry of Education of the People’s Republic of China,the NSFC(No.32071407 and No.62003023)+3 种基金the 111 Project(No.B13003)Innovation Research Plan from the Shanghai Municipal Education Commission(ZXWF082101/056)Exploratory Project of Natural Science Foundation of Shanghai(19ZR1476000)Beijing Advanced Innovation Center for Biomedical Engineering at Beihang University。
文摘Sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19.Here,we report a simple yet efficient platform integrating a portable and low-cost custom-made detector and a novel microwell array biochip for rapid and accurate detection of SARS-CoV-2.The instrument exhibits expedited amplification speed that enables colorimetric read-out within 25 minutes.A polymeric chip with a laser-engraved microwell array was developed to process the reaction between the primers and the respiratory swab RNA extracts,based on reverse transcriptase loop-mediated isothermal amplification(RT-LAMP).To achieve clinically acceptable performance,we synthesized a group of six primers to identify the conserved regions of the ORF1ab gene of SARS-CoV-2.Clinical trials were conducted with 87 PCR-positive and 43 PCRnegative patient samples.The platform demonstrated both high sensitivity(95.40%)and high specificity(95.35%),showing potentials for rapid and user-friendly diagnosis of COVID-19 among many other infectious pathogens.
基金supported by the National Natural Science Foundation of China(81900097,81903401)the Emergency Response Project of Hubei Science and Technology Department(2020FCA023)+3 种基金the Young Taishan Scholars Program of Shandong Province of China(tsqn20161046)the Shandong Province Higher Educational Young and Innovation Technology Supporting Program(2019KJL004)the Academic Promotion Program of Shandong First Medical University(2019RC010)the Emergency Diagnostic and Therapeutic Center of Central China.
文摘Background:The coronavirus disease 2019(COVID-19)has affected approximately 2 million individuals worldwide;however,data regarding fatal cases have been limited.Objective:To report the clinical features of 162 fatal cases of COVID-19 from 5 hospitals in Wuhan between December 30,2019 and March 12,2020.Methods:The demographic data,signs and symptoms,clinical course,comorbidities,laboratory findings,computed tomographic(CT)scans,treatments,and complications of the patients with fatal cases were retrieved from electronic medical records.Results:The median patient age was 69.5(interquartile range:63.0–77.25)years,and 80%of the patients were over 61 years.A total of 112(69.1%)patients were men.Hypertension(45.1%)was the most common comorbidity,while 59(36.4%)patients had no comorbidity.At admission,131(81.9%)patients had severe or critical COVID-19,whereas 39(18.1%)patients with hypertension or chronic lung disease had moderate COVID-19.In total,126(77.8%)patients received antiviral treatment,while 132(81.5%)patients received glucocorticoid treatment.A total of 116(71.6%)patients were admitted to the intensive care unit(ICU),and 137(85.1%)patients received mechanical ventilation.Most patients received mechanical ventilation before ICU admission.Approximately 93.2%of the patients developed respiratory failure or acute respiratory distress syndrome.There were no significant differences in the inhospital survival time among the hospitals(P=0.14).Conclusion:Young patients with moderate COVID-19 without comorbidity at admission could also develop fatal outcomes.The in-hospital survival time of the fatal cases was similar among the hospitals of different levels in Wuhan.
