A novel Er:YAG laser system operating at 1645 nm with high pulse-repetition-frequency(PRF)of kHz level is demonstrated.A ring cavity with double gain medium end-pumped by two fiber lasers is utilized to obtain high pu...A novel Er:YAG laser system operating at 1645 nm with high pulse-repetition-frequency(PRF)of kHz level is demonstrated.A ring cavity with double gain medium end-pumped by two fiber lasers is utilized to obtain high pulse energy.A novel‘triple-reflection’configuration on a piezoelectric actuator(PZT)is adopted to achieve high-repetition-rate at 3-kHz operation with the ramp-fire locking method.Single frequency pulses with maximum average power of 18.3 W at 3 kHz are obtained,and the pulse duration time is 318 ns.The full line width at half maximum(FWHM)of the pulses measured by the heterodyne technique is 1.71 MHz at 3 kHz.To the best of our knowledge,this is the highest PRF single-frequency laser pulses achieved based Er:YAG gain medium.展开更多
Verticillium dahliae is an important fungal pathogen affecting cotton yield and quality.Therefore,the mining of V.dahlia-resistance genes is urgently needed.Proteases and protease inhibitors play crucial roles in plan...Verticillium dahliae is an important fungal pathogen affecting cotton yield and quality.Therefore,the mining of V.dahlia-resistance genes is urgently needed.Proteases and protease inhibitors play crucial roles in plant defense responses.However,the functions and regulatory mechanisms of the protease inhibitor PR6 gene family remain largely unknown.This study provides a comprehensive analysis of the PR6 gene family in the cotton genome.We performed genome-wide identification and functional characterization of the cotton GhPR6 gene family,which belongs to the potato protease inhibitor I family of inhibitors.Thirty-nine PR6s were identified in Gossypium arboreum,G.raimondii,G.barbadense,and G.hirsutum,and they were clustered into four groups.Based on the analysis of pathogen-induced and Ghlmm transcriptome data,Gh PR6-5b was identified as the key gene for V.dahliae resistance.Virus-induced gene silencing experiments revealed that cotton was more sensitive to V.dahliae V991after PR6-5b silencing.The present study established that GhWRKY75 plays an important role in resistance to Verticillium wilt in cotton by positively regulating GhPR6-5b expression by directly binding to the W-box TTGAC(T/C).Our findings established that GhWRKY75 is a potential candidate for improving cotton resistance to V.dahliae,and provide primary information for further investigations and the development of specific strategies to bolster the defense mechanisms of cotton against V.dahliae.展开更多
We demonstrated a high-energy single-frequency erbium-doped yttrium aluminum garnet(Er:YAG)laser.With1470 nm laser diodes(LDs)as pumping sources,single-frequency laser pulses with energy of 28.6 m J,21.6 m J,and 15.0 ...We demonstrated a high-energy single-frequency erbium-doped yttrium aluminum garnet(Er:YAG)laser.With1470 nm laser diodes(LDs)as pumping sources,single-frequency laser pulses with energy of 28.6 m J,21.6 m J,and 15.0 m J are obtained at pulse repetition frequency of 200 Hz,300 Hz,and 500 Hz,respectively.As far as we know,this is the highest single-frequency pulse energy with the Er:YAG gain medium.With the ring cavity design,pulse duration is maintained at hundreds of nanoseconds.This high-energy single-frequency laser with hundreds of nanoseconds pulse duration is a prospective laser source for light detection and ranging applications.展开更多
A novel 1 kHz single-frequency, Q-switched Er-doped yttrium aluminum garnet(Er:YAG) laser pumped by a 1470 nm laser diode is demonstrated. The 500 ns, 5.52 mJ single-frequency, diffraction-limited pulses are obtained ...A novel 1 kHz single-frequency, Q-switched Er-doped yttrium aluminum garnet(Er:YAG) laser pumped by a 1470 nm laser diode is demonstrated. The 500 ns, 5.52 mJ single-frequency, diffraction-limited pulses are obtained by using a ‘ramp-fire' injection-seeding technique and an optical feedback architecture. The full width at half-maximum of the pulse spectrum is measured to be 1.47 MHz by using the heterodyne technique. The beam quality M2 factors are measured to be 1.18 and 1.24 in the x and y directions, respectively.展开更多
This paper report an 1645 nm Er：YAG laser resonantly pumped by an Er-fiber laser operating at 1532 rim. 1.78 W continous wave （CW） output power with a slope efficiency of 52% was obtained using a long ring cavity a...This paper report an 1645 nm Er：YAG laser resonantly pumped by an Er-fiber laser operating at 1532 rim. 1.78 W continous wave （CW） output power with a slope efficiency of 52% was obtained using a long ring cavity and an 1 at.% doped Er：YAG crystal. In Q-switched mode, the obtained pulse energy was 1.6 mJ at a repetition rate of 500 Hz; for 1 kHz repetition rate, 1.2 mJ output energy was obtained.展开更多
Objectives:Resistance to apoptosis in esophageal squamous cell carcinoma(ESCC)constitutes a significant impediment to treatment efficacy.Exploring alternative cell death pathways and their regulatory factors beyond ap...Objectives:Resistance to apoptosis in esophageal squamous cell carcinoma(ESCC)constitutes a significant impediment to treatment efficacy.Exploring alternative cell death pathways and their regulatory factors beyond apoptosis is crucial for overcoming drug resistance and enhancing therapeutic outcomes in ESCC.Methods:Mammalian Ste 20-like kinase 1(MST1)is implicated in regulating various cell deaths,including apoptosis,autophagy,and pyroptosis.Employing enhanced ascorbate peroxidase 2(APEX2)proximity labeling coupled with immunoprecipitation-mass spectrometry(IP-MS),we elucidated the interactomes of MST1 across these three cell death paradigms.Results:Proteomic profiling unveiled the functional roles and subcellular localization of MST1 and its interacting proteins during normal proliferation and various cell death processes.Notably,MST1 exhibited an expanded interactome during cell death compared to normal proliferation and chromosome remodeling functions consistently.In apoptosis,there was a notable increase of mitosis-associated proteins such as INCENP,ANLN,KIF23,SHCBP1 and SUPT16H,which interacted with MST1,alongside decreased expression of the pre-apoptotic protein STK3.During autophagy,the bindings of DNA repair-related proteins CBX8 and m6A reader YTHDC1 to MST1 were enhanced.In pyroptosis,LRRFIP2 and FLII which can inhibit pyroptosis increasingly binding to MST1.Conclusions:Our findings delineate potential mechanisms through which MST1 and its interactomes regulate cell death,paving the way for further investigation to validate and consolidate these observations.展开更多
基金the National Key Research and Development Program of China(Grant No.2017YFB0405203)the National Natural Science Foundation of China(Grant No.61627821).
文摘A novel Er:YAG laser system operating at 1645 nm with high pulse-repetition-frequency(PRF)of kHz level is demonstrated.A ring cavity with double gain medium end-pumped by two fiber lasers is utilized to obtain high pulse energy.A novel‘triple-reflection’configuration on a piezoelectric actuator(PZT)is adopted to achieve high-repetition-rate at 3-kHz operation with the ramp-fire locking method.Single frequency pulses with maximum average power of 18.3 W at 3 kHz are obtained,and the pulse duration time is 318 ns.The full line width at half maximum(FWHM)of the pulses measured by the heterodyne technique is 1.71 MHz at 3 kHz.To the best of our knowledge,this is the highest PRF single-frequency laser pulses achieved based Er:YAG gain medium.
基金supported by the National Key R&D Program of China(2022YFD1200300)the National Nature Science Youth Science Fund Project,China(31801412)+2 种基金the Key R&D Program of Shandong Province,China(2021LZGC026)the Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences,China(CXGC2023G02)the Shandong Provincial Program,China(WST2020011)。
文摘Verticillium dahliae is an important fungal pathogen affecting cotton yield and quality.Therefore,the mining of V.dahlia-resistance genes is urgently needed.Proteases and protease inhibitors play crucial roles in plant defense responses.However,the functions and regulatory mechanisms of the protease inhibitor PR6 gene family remain largely unknown.This study provides a comprehensive analysis of the PR6 gene family in the cotton genome.We performed genome-wide identification and functional characterization of the cotton GhPR6 gene family,which belongs to the potato protease inhibitor I family of inhibitors.Thirty-nine PR6s were identified in Gossypium arboreum,G.raimondii,G.barbadense,and G.hirsutum,and they were clustered into four groups.Based on the analysis of pathogen-induced and Ghlmm transcriptome data,Gh PR6-5b was identified as the key gene for V.dahliae resistance.Virus-induced gene silencing experiments revealed that cotton was more sensitive to V.dahliae V991after PR6-5b silencing.The present study established that GhWRKY75 plays an important role in resistance to Verticillium wilt in cotton by positively regulating GhPR6-5b expression by directly binding to the W-box TTGAC(T/C).Our findings established that GhWRKY75 is a potential candidate for improving cotton resistance to V.dahliae,and provide primary information for further investigations and the development of specific strategies to bolster the defense mechanisms of cotton against V.dahliae.
基金supported by the National Key Research and Development Program of China(No.2017YFB0405203)the National Natural Science Foundation of China(NSFC)(No.61627821)。
文摘We demonstrated a high-energy single-frequency erbium-doped yttrium aluminum garnet(Er:YAG)laser.With1470 nm laser diodes(LDs)as pumping sources,single-frequency laser pulses with energy of 28.6 m J,21.6 m J,and 15.0 m J are obtained at pulse repetition frequency of 200 Hz,300 Hz,and 500 Hz,respectively.As far as we know,this is the highest single-frequency pulse energy with the Er:YAG gain medium.With the ring cavity design,pulse duration is maintained at hundreds of nanoseconds.This high-energy single-frequency laser with hundreds of nanoseconds pulse duration is a prospective laser source for light detection and ranging applications.
基金supported by the National Natural Science Foundation of China(NSFC)(No.61627821)the National Key Research and Development Program of China(No.2017YFB0405203)
文摘A novel 1 kHz single-frequency, Q-switched Er-doped yttrium aluminum garnet(Er:YAG) laser pumped by a 1470 nm laser diode is demonstrated. The 500 ns, 5.52 mJ single-frequency, diffraction-limited pulses are obtained by using a ‘ramp-fire' injection-seeding technique and an optical feedback architecture. The full width at half-maximum of the pulse spectrum is measured to be 1.47 MHz by using the heterodyne technique. The beam quality M2 factors are measured to be 1.18 and 1.24 in the x and y directions, respectively.
文摘This paper report an 1645 nm Er：YAG laser resonantly pumped by an Er-fiber laser operating at 1532 rim. 1.78 W continous wave （CW） output power with a slope efficiency of 52% was obtained using a long ring cavity and an 1 at.% doped Er：YAG crystal. In Q-switched mode, the obtained pulse energy was 1.6 mJ at a repetition rate of 500 Hz; for 1 kHz repetition rate, 1.2 mJ output energy was obtained.
基金supported by the funds of Guangdong Basic and Applied Basic Research Foundation(2019B030302012)the National Key R&D Program of China(2021YFC2501001,2022YFC3401002)+3 种基金Shenzhen Medical Research Funds(C2303002)the National Natural Science Foundation of China(82341024,82172930,82203286)the Major Program of Shenzhen Bay Laboratory(S201101004)China Postdoctoral Science Foundation(BX20220214).
文摘Objectives:Resistance to apoptosis in esophageal squamous cell carcinoma(ESCC)constitutes a significant impediment to treatment efficacy.Exploring alternative cell death pathways and their regulatory factors beyond apoptosis is crucial for overcoming drug resistance and enhancing therapeutic outcomes in ESCC.Methods:Mammalian Ste 20-like kinase 1(MST1)is implicated in regulating various cell deaths,including apoptosis,autophagy,and pyroptosis.Employing enhanced ascorbate peroxidase 2(APEX2)proximity labeling coupled with immunoprecipitation-mass spectrometry(IP-MS),we elucidated the interactomes of MST1 across these three cell death paradigms.Results:Proteomic profiling unveiled the functional roles and subcellular localization of MST1 and its interacting proteins during normal proliferation and various cell death processes.Notably,MST1 exhibited an expanded interactome during cell death compared to normal proliferation and chromosome remodeling functions consistently.In apoptosis,there was a notable increase of mitosis-associated proteins such as INCENP,ANLN,KIF23,SHCBP1 and SUPT16H,which interacted with MST1,alongside decreased expression of the pre-apoptotic protein STK3.During autophagy,the bindings of DNA repair-related proteins CBX8 and m6A reader YTHDC1 to MST1 were enhanced.In pyroptosis,LRRFIP2 and FLII which can inhibit pyroptosis increasingly binding to MST1.Conclusions:Our findings delineate potential mechanisms through which MST1 and its interactomes regulate cell death,paving the way for further investigation to validate and consolidate these observations.
