The coupling of washing with adsorption process can be adopted for the treatment of soils contaminated with heavy metals pollution.However,the complex environment of soil and the competitive behavior of leaching chemi...The coupling of washing with adsorption process can be adopted for the treatment of soils contaminated with heavy metals pollution.However,the complex environment of soil and the competitive behavior of leaching chemicals considerably restrain adsorption capacity of adsorbent material during washing process,which demands a higher resistance of the adsorbents to interference.In this study,we synthesized strongly magnetic,high specific surface area(573.49 m^(2)/g)UiO66 composites(i.e.,UiO66-Fe_(3)O_(4))using hydrothermal process.The UiO66-Fe_(3)O_(4) was applied as an adsorbent during the ethylene diamine tetraacetic acid(EDTA)-assisted washing process of contaminated soil.The incorporation of UiO66-Fe_(3)O_(4)results in rapid heavy metal removal and recovery from the soil under low concentrations of washing agent(0.001 mol/L)with reduced residual heavy metal mobility of soil after remediation.Furthermore,UiO66-Fe_(3)O_(4)can quickly recollect by an external magnet,which offers a simple and inexpensive recovery method for heavy metals from contaminated soil.Overall,UiO66-Fe_(3)O_(4)configuration with EDTA-assisted washing process showed opportunities for heavy metals contaminated sites.展开更多
Failures in uterine spiral artery remodeling can lead to placental defects and subsequent preeclampsia,a leading cause of fetal and maternal mortality during pregnancy.N 6-methyladenosine(m^(6)A),the most abundant m R...Failures in uterine spiral artery remodeling can lead to placental defects and subsequent preeclampsia,a leading cause of fetal and maternal mortality during pregnancy.N 6-methyladenosine(m^(6)A),the most abundant m RNA modification,is dysregulated in samples with preeclampsia.However,whether and how m^(6)A regulates uterine spiral artery remodeling and leads to subsequent preeclampsia in vivo remains unexplored.In this study,we generated two m^(6)A deficiency mouse models:one with a trophoblast-specific knockout of the m^(6)A methyltransferase gene Mettl3,and another with a methyltransferase enzyme mutation.Using these models,we demonstrated that m^(6)A deficiency impaired extravillous trophoblasts(EVTs)infiltration into the uterine spiral arteries,and the remodeling of the spiral arteries in vivo.We further showed that m^(6)A inhibition induced preeclampsia-like symptoms.Mechanistically,we revealed that the m^(6)A modification of FGF2 mRNA,which encodes a secreted peptide implicated in preeclampsia,facilitated its expression.Notably,administration of the FGF2 peptide largely restored EVTs invasion and uterine spiral artery remodeling in m^(6)A-deficient mice.Our findings underscore the importance of m^(6)A in facilitating uterine spiral artery remodeling and prove the pathological mechanisms in vivo,suggesting a new therapeutic approach for preeclampsia caused by m^(6)A deficiency.展开更多
Refrigerator insulation replacement results in discarding a large amount of waste refrigerator polyurethane(WRPU).Insect larvae like mealworms have been used to biodegrade pristine plastics.However,knowledge about mea...Refrigerator insulation replacement results in discarding a large amount of waste refrigerator polyurethane(WRPU).Insect larvae like mealworms have been used to biodegrade pristine plastics.However,knowledge about mealworms degrading WRPU is scarce.This study presents an in-depth investigation of the degradation of WRPU by mealworms using the micro-morphology,composition,and functional groups of WRPU and the egested frass characteristics.It was found that the WRPU debris in frass was scoured,implying that WRPU was ingested and degraded by mealworms.The carbon content of WRPU-based frass was lower than that of WRPU,indicating that mealworms utilized WRPU as a carbon source.The urethane groups in WRPU were broken,and benzene rings’C=C and C–H bonds in the isocyanate disappeared after being ingested by mealworms.Thermal gravimetric-differential thermal gravimetry analysis showed that the weight loss temperature of WRPU-based frass was 300°C lower than that of WRPU,indicating that the thermal stability of WRPU deteriorated after being ingested.The carbon balance analysis confirmed that carbon in the ingested WRPU released as CO_(2) increased from 18.84%to 29.80%,suggesting that WRPU was partially mineralized.The carbon in the mealworm biomass ingesting WRPU decreased.The possible reason is that WRPU does not supply sufficient nutrients for mealworm growth,and the impurities and odor present in WRPU affect the appetite of the mealworms.The microbial community analysis indicated that WRPU exerts a considerable effect on the gut microorganism of mealworms.These findings confirm that mealworms degrade WRPU.展开更多
基金supported by the University Basic Research Fund of China(No.2232020A-10)the Joint Foundation of Iron and Steel,National Natural Science Foundation of China(No.U1660107)。
文摘The coupling of washing with adsorption process can be adopted for the treatment of soils contaminated with heavy metals pollution.However,the complex environment of soil and the competitive behavior of leaching chemicals considerably restrain adsorption capacity of adsorbent material during washing process,which demands a higher resistance of the adsorbents to interference.In this study,we synthesized strongly magnetic,high specific surface area(573.49 m^(2)/g)UiO66 composites(i.e.,UiO66-Fe_(3)O_(4))using hydrothermal process.The UiO66-Fe_(3)O_(4) was applied as an adsorbent during the ethylene diamine tetraacetic acid(EDTA)-assisted washing process of contaminated soil.The incorporation of UiO66-Fe_(3)O_(4)results in rapid heavy metal removal and recovery from the soil under low concentrations of washing agent(0.001 mol/L)with reduced residual heavy metal mobility of soil after remediation.Furthermore,UiO66-Fe_(3)O_(4)can quickly recollect by an external magnet,which offers a simple and inexpensive recovery method for heavy metals from contaminated soil.Overall,UiO66-Fe_(3)O_(4)configuration with EDTA-assisted washing process showed opportunities for heavy metals contaminated sites.
基金supported by the National Natural Science Foundation of China(82230053,32222016)the National Key Research and Development Program of China(2019YFA0802300,2018YFC1004100)+1 种基金the Discipline Construction Project of Guangdong Medical University(4SG23026G)the Science and Technology Program of Guangzhou,China(202201010922)。
文摘Failures in uterine spiral artery remodeling can lead to placental defects and subsequent preeclampsia,a leading cause of fetal and maternal mortality during pregnancy.N 6-methyladenosine(m^(6)A),the most abundant m RNA modification,is dysregulated in samples with preeclampsia.However,whether and how m^(6)A regulates uterine spiral artery remodeling and leads to subsequent preeclampsia in vivo remains unexplored.In this study,we generated two m^(6)A deficiency mouse models:one with a trophoblast-specific knockout of the m^(6)A methyltransferase gene Mettl3,and another with a methyltransferase enzyme mutation.Using these models,we demonstrated that m^(6)A deficiency impaired extravillous trophoblasts(EVTs)infiltration into the uterine spiral arteries,and the remodeling of the spiral arteries in vivo.We further showed that m^(6)A inhibition induced preeclampsia-like symptoms.Mechanistically,we revealed that the m^(6)A modification of FGF2 mRNA,which encodes a secreted peptide implicated in preeclampsia,facilitated its expression.Notably,administration of the FGF2 peptide largely restored EVTs invasion and uterine spiral artery remodeling in m^(6)A-deficient mice.Our findings underscore the importance of m^(6)A in facilitating uterine spiral artery remodeling and prove the pathological mechanisms in vivo,suggesting a new therapeutic approach for preeclampsia caused by m^(6)A deficiency.
基金the National Key R&D Program of China(Nos.2019YFC0408204,2018YFC1903201,and 2018YFC0213605)the Shanghai Committee of Science and Technology(China)(No.19DZ1204702)+1 种基金the National Natural Scientific Foundation of China(No.52070126)the Joint Projects of Shanghai University grant(China)202142.
文摘Refrigerator insulation replacement results in discarding a large amount of waste refrigerator polyurethane(WRPU).Insect larvae like mealworms have been used to biodegrade pristine plastics.However,knowledge about mealworms degrading WRPU is scarce.This study presents an in-depth investigation of the degradation of WRPU by mealworms using the micro-morphology,composition,and functional groups of WRPU and the egested frass characteristics.It was found that the WRPU debris in frass was scoured,implying that WRPU was ingested and degraded by mealworms.The carbon content of WRPU-based frass was lower than that of WRPU,indicating that mealworms utilized WRPU as a carbon source.The urethane groups in WRPU were broken,and benzene rings’C=C and C–H bonds in the isocyanate disappeared after being ingested by mealworms.Thermal gravimetric-differential thermal gravimetry analysis showed that the weight loss temperature of WRPU-based frass was 300°C lower than that of WRPU,indicating that the thermal stability of WRPU deteriorated after being ingested.The carbon balance analysis confirmed that carbon in the ingested WRPU released as CO_(2) increased from 18.84%to 29.80%,suggesting that WRPU was partially mineralized.The carbon in the mealworm biomass ingesting WRPU decreased.The possible reason is that WRPU does not supply sufficient nutrients for mealworm growth,and the impurities and odor present in WRPU affect the appetite of the mealworms.The microbial community analysis indicated that WRPU exerts a considerable effect on the gut microorganism of mealworms.These findings confirm that mealworms degrade WRPU.
