With rapid developments of emerging technologies like synthetic biology,the demand for DNA polymerases with superior activities including higher thermostability and processivity has increased significantly.Thus,ration...With rapid developments of emerging technologies like synthetic biology,the demand for DNA polymerases with superior activities including higher thermostability and processivity has increased significantly.Thus,rational optimization of the performance of DNA polymerase is of great interest.Nuclear magnetic resonance(NMR)spectroscopy is a powerful technique used for studying protein structure and dynamics.It provides the atomic resolution information of enzymes under their functional solution environment to reveal the active sites(hot spots)of the enzyme,which could be further used for optimizing the performance of enzymes.In our previous work,we identified hot spot residues of Pyrococcus furiosus DNA polymerase(Pfu pol).We aim to employ these binding hot spots to screen for co-factors of Pfu pol,particularly targeting those molecules exhibiting weak intermolecular interactions.To validate this concept,we first demonstrated the feasibility of utilizing hot spot residues as screening probes for auxiliary factors by employing the well-characterized Tween-20 as a model system.Employing these hot spots as probes,two new co-factors,the heat shock protein TkHSP20 from Thermococcus Kodakaraensis and the chemical chaperone L-arginine,are identified to interact with Pfu pol to boost its performance in amplifying long DNA fragments by enhancing the thermal stability and the processivity of the Pfu pol.This NMR-based approach requires no prior assignment information of target enzymes,guiding the rational exploration of novel cofactors for Pfu pol.Moreover,our approach is not dependent on structural data or bioinformatics.Therefore,it has significant potential for application in various enzymes to expedite the progress in enzyme engineering.展开更多
With the rapid development of the world economy,IGBT has been widely used in motor drive and electric energy conversion.In order to timely detect the fatigue damage of IGBT,it is necessary to monitor the junction temp...With the rapid development of the world economy,IGBT has been widely used in motor drive and electric energy conversion.In order to timely detect the fatigue damage of IGBT,it is necessary to monitor the junction temperature of IGBT.In order to realize the fast calculation of IGBT junction temperature,a finite element method of IGBT temperature field reduction is proposed in this paper.Firstly,the finite element calculation process of IGBT temperature field is introduced and the linear equations of finite element calculation of temperature field are derived.Temperature field data of different working conditions are obtained by finite element simulation to form the sample space.Then the covariance matrix of the sample space is constructed,whose proper orthogonal decomposition and modal extraction are carried out.Reasonable basis vector space is selected to complete the low dimensional expression of temperature vector inside and outside the sample space.Finally,the reduced-order model of temperature field finite element is obtained and solved.The results of the reduced order model are compared with those of the finite element method,and the performance of the reduced-order model is evaluated from two aspects of accuracy and rapidity.展开更多
Objective:This work aimed to present a descriptive analysis of the outcome measures used in clinical trials of traditional Chinese medicine(TCM)for patients with stable angina pectoris,and to provide baseline data for...Objective:This work aimed to present a descriptive analysis of the outcome measures used in clinical trials of traditional Chinese medicine(TCM)for patients with stable angina pectoris,and to provide baseline data for the development of core outcome sets(COSs)for relevant clinical trials.Methods:Medical databases were searched to identify randomized trials of the effects of TCM for the treatment of stable angina pectoris.Outcome measures of each trial were extracted.Descriptive statistics were used to analyze the baseline characteristics of outcomes in clinical trials of TCM.Results:94 randomized trials(with 9,111 participants)involving 79 different outcomes were identified.The mean number of outcomes was 5(1-21 per trial).The 5 most commonly reported outcomes were efficacy rate of electrocardiogram,efficacy rate of angina pectoris,efficacy rate of TCM syndrome,fasting lipid indices,and withdrawal rate of nitroglycerin.Several challenges were identified:(1)significant heterogeneity of outcomes and differences in the technique and timing of the measurement of the same outcome;(2)transformation of continuous data into categorical data and presented as such in>90% of trials;(3)few trials on the outcomes associated with the advantages and characteristics of TCM;and(4)selective reporting of outcomes.Conclusions:The outcomes used are excessively heterogenous,and the choice of some outcomes(timing and techniques)for measurement is confusing or inappropriate.Hence,developing and implementing a COS is necessary for greater consistency.展开更多
Modifying electrochemical surface area(ECSA)and surface chemistry are promising approaches to enhance the capacities of oxygen cathodes for lithium-oxygen(Li-O_(2))batteries.Although various chemical approaches have b...Modifying electrochemical surface area(ECSA)and surface chemistry are promising approaches to enhance the capacities of oxygen cathodes for lithium-oxygen(Li-O_(2))batteries.Although various chemical approaches have been successfully used to tune the cathode surface,versatile physical techniques including plasma etching etc.could be more effortless and effective than arduous chemical treatments.Herein,for the first time,we propose a facile oxygen plasma treatment to simultaneously etch and modify the surface of Co_(3)O_(4)nanosheet arrays(NAs)cathode for Li-O_(2)batteries.The oxygen plasma not only etches Co_(3)O_(4)nanosheets to enhance the ECSA but also lowers the oxygen vacancy concentration to enable a Co^(3+)-rich surface.In addition,the NA architecture enables the full exposure of oxygen vacancies and surface Co^(3+)that function as the catalytically active sites.Thus,the synergistic effects of enhanced ECSA,modest oxygen vacancy and high surface Co^(3+)achieve a significantly enhanced reversible capacity of 3.45 mAh/cm^(2)for Co_(3)O_(4)NAs.This work not only develops a promising high-capacity cathode for Li-O_(2)batteries,but also provides a facile physical method to simultaneously tune the nanostructure and surface chemistry of energy storage materials.展开更多
The recent initiative by China’s National Medical Products Administration(NMPA)to realign traditional Chinese medicine(TCM)registration with clinical value marks a pivotal shift toward modernizing and enhancing the e...The recent initiative by China’s National Medical Products Administration(NMPA)to realign traditional Chinese medicine(TCM)registration with clinical value marks a pivotal shift toward modernizing and enhancing the efficacy of TCM.This initiative reflects a progressive attitude toward integrating TCM’s rich heritage with contemporary medical needs,ensuring that it remains relevant and effective in addressing modern health challenges[1].展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences XDB0540000Natural Science Foundation of China grants 22327901,22174151 and 21991080Hubei Provincial Natural Science Foundation of China 2023AFA041。
文摘With rapid developments of emerging technologies like synthetic biology,the demand for DNA polymerases with superior activities including higher thermostability and processivity has increased significantly.Thus,rational optimization of the performance of DNA polymerase is of great interest.Nuclear magnetic resonance(NMR)spectroscopy is a powerful technique used for studying protein structure and dynamics.It provides the atomic resolution information of enzymes under their functional solution environment to reveal the active sites(hot spots)of the enzyme,which could be further used for optimizing the performance of enzymes.In our previous work,we identified hot spot residues of Pyrococcus furiosus DNA polymerase(Pfu pol).We aim to employ these binding hot spots to screen for co-factors of Pfu pol,particularly targeting those molecules exhibiting weak intermolecular interactions.To validate this concept,we first demonstrated the feasibility of utilizing hot spot residues as screening probes for auxiliary factors by employing the well-characterized Tween-20 as a model system.Employing these hot spots as probes,two new co-factors,the heat shock protein TkHSP20 from Thermococcus Kodakaraensis and the chemical chaperone L-arginine,are identified to interact with Pfu pol to boost its performance in amplifying long DNA fragments by enhancing the thermal stability and the processivity of the Pfu pol.This NMR-based approach requires no prior assignment information of target enzymes,guiding the rational exploration of novel cofactors for Pfu pol.Moreover,our approach is not dependent on structural data or bioinformatics.Therefore,it has significant potential for application in various enzymes to expedite the progress in enzyme engineering.
基金This work was supported by the National Natural Science Foundation of China(82004505)the National Key Research and Development Program of the Ministry of Science and Technology of China(2020YFC0841600)Henan Province Coronavirus Emergency Prevention and Control Special Research(201100310400,201003050).
基金supported in part by Heilongjiang Provincial Natural Science Foundation of China under Project TD2021E004in part by Ningbo Science and Technology Bureau under S&T Innovation 2025 Major Special Programme with project code 2019B10071。
文摘With the rapid development of the world economy,IGBT has been widely used in motor drive and electric energy conversion.In order to timely detect the fatigue damage of IGBT,it is necessary to monitor the junction temperature of IGBT.In order to realize the fast calculation of IGBT junction temperature,a finite element method of IGBT temperature field reduction is proposed in this paper.Firstly,the finite element calculation process of IGBT temperature field is introduced and the linear equations of finite element calculation of temperature field are derived.Temperature field data of different working conditions are obtained by finite element simulation to form the sample space.Then the covariance matrix of the sample space is constructed,whose proper orthogonal decomposition and modal extraction are carried out.Reasonable basis vector space is selected to complete the low dimensional expression of temperature vector inside and outside the sample space.Finally,the reduced-order model of temperature field finite element is obtained and solved.The results of the reduced order model are compared with those of the finite element method,and the performance of the reduced-order model is evaluated from two aspects of accuracy and rapidity.
基金supported by the National Natural Science Foundation Project of China(81473544)Ministry of Education Program for Innovative Research Team(IRT1276)+2 种基金National key research and development projects of modernization of traditional Chinese medicine(2019YFC1710000,2019YFC1710003)Henan Youth Talent Promotion Project(2020HYTP060)the National Basic Research Program of China(2015CB554401).
文摘Objective:This work aimed to present a descriptive analysis of the outcome measures used in clinical trials of traditional Chinese medicine(TCM)for patients with stable angina pectoris,and to provide baseline data for the development of core outcome sets(COSs)for relevant clinical trials.Methods:Medical databases were searched to identify randomized trials of the effects of TCM for the treatment of stable angina pectoris.Outcome measures of each trial were extracted.Descriptive statistics were used to analyze the baseline characteristics of outcomes in clinical trials of TCM.Results:94 randomized trials(with 9,111 participants)involving 79 different outcomes were identified.The mean number of outcomes was 5(1-21 per trial).The 5 most commonly reported outcomes were efficacy rate of electrocardiogram,efficacy rate of angina pectoris,efficacy rate of TCM syndrome,fasting lipid indices,and withdrawal rate of nitroglycerin.Several challenges were identified:(1)significant heterogeneity of outcomes and differences in the technique and timing of the measurement of the same outcome;(2)transformation of continuous data into categorical data and presented as such in>90% of trials;(3)few trials on the outcomes associated with the advantages and characteristics of TCM;and(4)selective reporting of outcomes.Conclusions:The outcomes used are excessively heterogenous,and the choice of some outcomes(timing and techniques)for measurement is confusing or inappropriate.Hence,developing and implementing a COS is necessary for greater consistency.
基金supported by grants from the National Natural Science Foundation of China(Nos.22075219 and 51972257)the National Key Research Program of China(No.2016YFA0202602)the Research Start-Up Fund from Wuhan University of Technology.
文摘Modifying electrochemical surface area(ECSA)and surface chemistry are promising approaches to enhance the capacities of oxygen cathodes for lithium-oxygen(Li-O_(2))batteries.Although various chemical approaches have been successfully used to tune the cathode surface,versatile physical techniques including plasma etching etc.could be more effortless and effective than arduous chemical treatments.Herein,for the first time,we propose a facile oxygen plasma treatment to simultaneously etch and modify the surface of Co_(3)O_(4)nanosheet arrays(NAs)cathode for Li-O_(2)batteries.The oxygen plasma not only etches Co_(3)O_(4)nanosheets to enhance the ECSA but also lowers the oxygen vacancy concentration to enable a Co^(3+)-rich surface.In addition,the NA architecture enables the full exposure of oxygen vacancies and surface Co^(3+)that function as the catalytically active sites.Thus,the synergistic effects of enhanced ECSA,modest oxygen vacancy and high surface Co^(3+)achieve a significantly enhanced reversible capacity of 3.45 mAh/cm^(2)for Co_(3)O_(4)NAs.This work not only develops a promising high-capacity cathode for Li-O_(2)batteries,but also provides a facile physical method to simultaneously tune the nanostructure and surface chemistry of energy storage materials.
文摘The recent initiative by China’s National Medical Products Administration(NMPA)to realign traditional Chinese medicine(TCM)registration with clinical value marks a pivotal shift toward modernizing and enhancing the efficacy of TCM.This initiative reflects a progressive attitude toward integrating TCM’s rich heritage with contemporary medical needs,ensuring that it remains relevant and effective in addressing modern health challenges[1].
