The study on sand body connectivity and distribution patterns is of great significance for well emplacement and injection-production pattern analysis in the A oilfield of the Weixi?nan Sag currently at a pre-developme...The study on sand body connectivity and distribution patterns is of great significance for well emplacement and injection-production pattern analysis in the A oilfield of the Weixi?nan Sag currently at a pre-development stage.Based on the current drilling data,seismic data,and fault development characteristics,this study investigates the connectivity,geometric morphology,planar distribution,and vertical evolution of composite sand bodies(multi-stage superimposed channel sand bodies)within the fault block using seismic forward and inversion modeling.The El3I oil layer group in the third member of the Liushagang Formation is developed in the fan delta-front sub-facies,which mainly consists of subaqueous distributary channels.The thickness of single-stage subaqueous distributary channel sand bodies ranges from 2 to 6 m,and the width of composite channel sand bodies varies from 50 to 100 m.Under the long-term transgression background,the subaqueous distributary channels in the El3I oil layer group are relatively narrow,forming superimposed and continuous composite channel sand bodies through lateral migration and vertical stacking.The long-term base-level cycles control the width of subaqueous distributary channels,while the mid-term base-level cycles control the thickness of these channels.The subaqueous distributary channels developed during the late stage of mid-term base-level fall are thicker than those formed during the early stage.Accordingly,quantitative relationships between channel thickness and width are established for the early and late stages of mid-term base-level fall,to finely depict the evolution patterns of channel sand body geometry and stacking styles across different stages.These findings provide important guidance for accurately predicting the planar distribution and channel width of composite subaqueous distributary channels at different stages of the mid-term baselevel cycles.展开更多
Multi-axle heavy-duty vehicles(MHVs)are essential for military equipment transport due to their safety and stability.However,braking dynamic responses between MHVs and pavement systems still remain underexplored,parti...Multi-axle heavy-duty vehicles(MHVs)are essential for military equipment transport due to their safety and stability.However,braking dynamic responses between MHVs and pavement systems still remain underexplored,particularly regarding their complex load transfer mechanisms.This paper develops an enhanced model of a multi-axle heavy-duty vehicle(MHV)coupled with the uneven and flexible pavement.An advanced coupling iterative method is proposed to solve the highly dimensional equations of the MHV-pavement coupled system.The proposed method was validated through experimental tests,with characteristic parameters of vertical accelerations showing relative errors between 0.42%and 11.80%.The coupling effect and influence mechanism of the braking process are investigated by characteristic parameters of the dynamic responses.Additionally,the influences of braking conditions and pavement parameters are analyzed in time and frequency domains in order to reveal the vibration mechanisms of the coupled system.Moreover,this study establishes a theoretical foundation for monitoring pavement health via vehicle-mounted acceleration signals,which is necessary in military transportation.展开更多
Tight glutenite reservoirs are known for strong heterogeneity,complex wettability,and challenging development.Gas-Assisted Gravity Drainage(GAGD)technology has the potential to significantly improve recovery efficienc...Tight glutenite reservoirs are known for strong heterogeneity,complex wettability,and challenging development.Gas-Assisted Gravity Drainage(GAGD)technology has the potential to significantly improve recovery efficiency in glutenite reservoir.However,there is currently limited research on GAGD processes specifically designed for glutenite reservoirs,and there is a lack of relevant dimensionless numbers for predicting recovery efficiency.In this study,we developed a theoretical model based on the characteristics of glutenite reservoirs and used phase-field method to track the oil-gas interface for numerical simulations of dynamic GAGD processes.To explore the factors influencing gas-driven recovery,we simulated the effects of strong heterogeneity and dynamic wettability on the construction process under gravity assistance.Additionally,we introduced multiple dimensionless numbers(including capillary number,viscosity ratio,and Bond number)and conducted a series of numerical simulations.The results demonstrate that gravity enhances the stability of the oil-gas interface but causes unstable pressure fluctuations when passing through different-sized throat regions,particularly leading to front advancement in smaller throats.Although strong heterogeneity has negative impacts on GAGD,they can be mitigated by reducing injection velocity.Increasing oil-wettability promotes oil displacement by overcoming capillary forces,particularly in narrower pores,allowing residual oils to be expelled.Among the dimensionless numbers,the recovery efficiency is directly proportional to the Bond number and inversely proportional to the capillary number and viscosity ratio.Through sensitivity analysis of the dimensionless numbers’impact on the recovery efficiency,a new dimensionless N_(Glu) considering heterogeneity is proposed to accurately predict GAGD recovery of tight glutenite reservoirs.展开更多
The compositions and contents of soluble sugars highly determine the flavor and quality of fleshy fruits.In the present study,we found that the overexpression of transcription factor MdWRKY126 localized on the nucleus...The compositions and contents of soluble sugars highly determine the flavor and quality of fleshy fruits.In the present study,we found that the overexpression of transcription factor MdWRKY126 localized on the nucleus enhanced sucrose concentration while decreased fructose and glucose concentration in transgenic apple calli and ripening tomato fruits.To comprehensively understand the effects of the MdWRKY126 on the content of various soluble sugars in apple and tomato fruits,enzyme activities and related essential genes associated with the sugar metabolism and transportation pathway in MdWRKY126-overrexpressed apple and tomato lines were analyzed.The results indicated that the overexpression of MdWRKY126 upregulated sucrose phosphate synthase(SPS)activity and the gene expression levels of SPS and sucrose transporter SUT,which was conducive to a large accumulation of sucrose in fruit cells.Meanwhile,MdWRKY126 overexpression downregulated the activity of enzymes involved in sucrose decomposition including cell wall invertase(CWINV),sucrose synthase(SUSY)and the corresponding gene expressions,as well as inhibited the expression levels of hexose transporter(HTs)and tonoplast sugar transporter(TSTs)that transport hexose into vacuoles,resulting in a reduced hexose level in apple calli and tomato fruit.These findings enrich our understanding of the metabolism and regulation of soluble sugars in apple fruits.展开更多
Both sorbitol and sucrose are synthesized in source leaves and transported to fruit for supporting fruit growth in tree fruit species of the Rosaceae family.In apple(Malus domestica),antisense suppression of aldose-6-...Both sorbitol and sucrose are synthesized in source leaves and transported to fruit for supporting fruit growth in tree fruit species of the Rosaceae family.In apple(Malus domestica),antisense suppression of aldose-6-phosphate reductase,the key enzyme for sorbitol synthesis,significantly decreased the sorbitol concentration but increased the sucrose concentration in leaves,leading to a lower sorbitol but a higher sucrose supply to fruit in these plants.In response to this altered carbon supply,the transgenic fruit had lower concentration of sorbitol and much higher concentration of glucose but similar levels of fructose,sucrose,and starch throughout fruit development relative to the untransformed control.Activities of sorbitol dehydrogenase,fructokinase,and sucrose phosphate synthase were lower,whereas activities of neutral invertase,sucrose synthase,and hexokinase were higher in the transgenic fruit during fruit development.Transcript levels of MdSOT1,MdSDHs,MdFK2,and MdSPS3/6 were downregulated,whereas transcript levels of MdSUC1/4,MdSUSY1-3,MdNIV1/3,MdHKs,and MdTMT1 were upregulated in the transgenic fruit.These findings suggest that the Sucrose cycle and the sugar transport system are very effective in maintaining the level of fructose and provide insights into the roles of sorbitol and sucrose in regulating sugar metabolism and accumulation in sorbitol-synthesizing species.展开更多
Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)are the most typical pathogenic bacteria with a significantly high risk of bio-contamination,widely existing in hospital and public places.Recent studies on a...Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)are the most typical pathogenic bacteria with a significantly high risk of bio-contamination,widely existing in hospital and public places.Recent studies on antibacterial materials and the related mechanisms have attracted more interests of researchers.However,the antibacterial behavior of materials is usually evaluated separately on the single bacterial strain,which is far from the practical condition.Actually,the interaction between the polymicrobial communities can promote the growing profile of bacteria,which may weaken the antibacterial effect of materials.In this work,a 420 copper-bearing martensitic stainless steel(420 CuSS)was studied with respect to its antibacterial activity and the underlying mechanism in a co-culturing infection model using both E.coli and S.au reus.Observed via plating and counting colony forming units(CFU),Cu releasing,and material characterization,420 CuSS was proved to present excellent antibacterial performance against the mixed bacteria with an approximately 99.4%of antibacterial rate.In addition,420 CuSS could effectively inhibit the biofilm formation on its surfaces,resulting from a synergistic antibacterial effect of Cu ions,Fe ions,reactive oxygen species(ROS),and proton consumption of bacteria.展开更多
To investigate the functions of fructokinase(FRK)in apple(Malus domestica)carbohydrate metabolism,we cloned the coding sequences of MdFRK1 and MdFRK2 from the‘Royal Gala’apple.The results showed that MdFRK2 expressi...To investigate the functions of fructokinase(FRK)in apple(Malus domestica)carbohydrate metabolism,we cloned the coding sequences of MdFRK1 and MdFRK2 from the‘Royal Gala’apple.The results showed that MdFRK2 expression was extremely high in shoot tips and young fruit.Analyses of heterologously expressed proteins revealed that MdFRK2 had a higher affinity for fructose than did MdFRK1,with Km values of 0.1 and 0.62 mM for MdFRK2 and MdFRK1,respectively.The two proteins,however,exhibited similar Vmax values when their activities were significantly inhibited by high concentrations of fructose.MdFRK2 ectopic expression was associated with a general decrease in fructose concentration in transgenic lines.In leaves,increased FRK activity similarly resulted in reduced concentrations of glucose and sucrose but no alterations in sorbitol concentration.When compared with those in the untransformed control,genes involved in sorbitol synthesis(A6PR)and the degradation pathway(SDH1/2)were significantly upregulated in transgenic lines,whereas those involved in sucrose synthesis(SPS1)and other degradation processes(SUSY4,NINV1/2,and HxK2)were downregulated.The activity of enzymes participating in carbohydrate metabolism was proportional to the level of gene expression.However,the growth performance and photosynthetic efficiency did not differ between the transgenic and wild-type plants.These results provide new genetic evidence to support the view that FRK plays roles in regulating sugar and sorbitol metabolism in Rosaceae plants.展开更多
Fruits from three cultivars of apple(Malus × domestica Borkh.)—‘McIntosh’,‘Gala’and‘Mutsu’—were harvested from the exterior and interior of the tree canopy.Peel and flesh tissues were sampled separately t...Fruits from three cultivars of apple(Malus × domestica Borkh.)—‘McIntosh’,‘Gala’and‘Mutsu’—were harvested from the exterior and interior of the tree canopy.Peel and flesh tissues were sampled separately to determine how the position of the fruit on the tree might affect the levels of the primary and secondary metabolites in the fruit.Fruit from the outer-canopy had a higher fresh weight and a higher soluble solids content compared with inner-canopy fruit.Both the flesh and peel of the outer-canopy fruit had higher concentrations of soluble sugars and sugar alcohols,but lower starch concentrations than the inner-canopy fruit.Canopy position did not significantly affect malic acid concentrations,except in the peel of‘McIntosh’and the flesh of‘Mutsu’.Although levels of ascorbic and succinic acids were higher in the peel of the outer-canopy fruit,the responses of other organic acids to canopy position depended on tissue type and cultivar.Except for histidine,lysine,threonine and glycine,most amino acids accumulated at higher concentrations in the inner-canopy fruit.By contrast,levels of phenolic compounds from both the peel and flesh were significantly higher in the outer-canopy fruit.The significant effects of location within the canopy on both primary metabolites and secondary metabolites demonstrate the importance of light exposure on apple fruit quality.展开更多
Cold atmospheric plasmas(CAPs)have shown great applicability in agriculture.Many kinds of CAP sources have been studied in agricultural applications to promote plant growth and cure plant diseases.We briefly review th...Cold atmospheric plasmas(CAPs)have shown great applicability in agriculture.Many kinds of CAP sources have been studied in agricultural applications to promote plant growth and cure plant diseases.We briefly review the state-of-the-art stimulating effects of atmospheric-pressure dielectricbarrier-discharge(AP-DBD)plasmas,after the direct or indirect treatment of plants for growth promotion and disease control.We then discuss the special demands on the characteristics of the CAP sources for their applications in plant mutation breeding.An atmospheric and room temperature plasma(ARTP)jet generator with a large plasma irradiation area,a high enough concentration of chemically reactive species and a low gas temperature is designed for direct plant mutagenesis.Experimental measurements of the electrical,thermal and optical features of the ARTP generator are conducted.Then,an ARTP-P(ARTP for plant mutagenesis)mutation breeding machine is developed,and a typical case of plant mutation breeding by the ARTP-P mutation machine is presented using Coreopsis tinctoria Nutt.seeds.Physical and agricultural experiments show that the newly-developed ARTP-P mutation breeding machine with a large irradiation area can generate uniform CAP jets with high concentrations of chemically reactive species and mild gas temperatures,and have signiflcant mutagenesis effects on the Coreopsis tinctoria Nutt.seeds.The ARTP-P mutation breeding machine may provide a platform for systematic studies on mutation mechanisms and results for various plant seeds under different operating conditions in future research.展开更多
This study is to numerically test the interfacial instability of ferrofluid flow under the presence of a vacuum magnetic field.The ferrofluid parabolized stability equations(PSEs)are derived from the ferrofluid stabil...This study is to numerically test the interfacial instability of ferrofluid flow under the presence of a vacuum magnetic field.The ferrofluid parabolized stability equations(PSEs)are derived from the ferrofluid stability equations and the Rosensweig equations,and the characteristic values of the ferrofluid PSEs are given to describe the ellipticity of ferrofluid flow.Three numerical models representing specific cases considering with/without a vacuum magnetic field or viscosity are created to mathematically examine the interfacial instability by the computation of characteristic values.Numerical investigation shows strong dependence of the basic characteristic of ferrofluid Rayleigh-Taylor instability(RTI)on viscosity of ferrofluid and independence of the vacuum magnetic field.For the shock wave striking helium bubble,the magnetic field is not able to trigger the symmetry breaking of bubble but change the speed of the bubble movement.In the process of droplet formation from a submerged orifice,the collision between the droplet and the liquid surface causes symmetry breaking.Both the viscosity and the magnetic field exacerbate symmetry breaking.The computational results agree with the published experimental results.展开更多
Soluble sugars function not only as the energy and structural blocks supporting plants,but also as osmoregulators and signal molecules during plant adaptation to water deficit.Here,we investigated drought resistance i...Soluble sugars function not only as the energy and structural blocks supporting plants,but also as osmoregulators and signal molecules during plant adaptation to water deficit.Here,we investigated drought resistance in transgenic apple(Malus×domestica)overexpressing MdFRK2,a key gene regulating fructose content and sugar metabolism.There is no obvious phenotypic difference between MdFRK2-overexpressing transgenic plants and WT plants under the well-watered condition.However,the transgenic plants and the grafted plants using MdFRK2-overexpressing rootstock exhibited improved tolerance to drought stress.Overexpression of MdFRK2 significantly promoted the growth of root system under drought stress.RNA sequencing showed that under drought stress,genes involved in sugar metabolism,transcription regulation,signal transduction or hormone metabolism were differentially expressed in MdFRK2 transgenic plants.Consistent with the gene expression profile,the activities of enzyme(SDH,FRK and NI)involved in sugar metabolism in the roots of MdFRK2 transgenic plants were significantly higher than those of untransformed control plants after drought stress.Under drought stress,overexpression of MdFRK2 promoted the accumulation of IAA,and decreased the contents of ABA and CK in apple root system.In conclusion,these results suggest that MdFRK2 can promote the growth of apple roots under drought stress by regulating sugar metabolism and accumulation,hormone metabolism and signal transduction,and then resist drought stress.展开更多
Based on the Zufiria theoretical model, a new model regarding the asymptotic bubble velocity for the Rayleigh-Taylor （RT） instability is presented by use of the complex velocity potential proposed by Sohn. The propo...Based on the Zufiria theoretical model, a new model regarding the asymptotic bubble velocity for the Rayleigh-Taylor （RT） instability is presented by use of the complex velocity potential proposed by Sohn. The proposed model is an extension of the ordinary Zufiria model and can deal with non-ideal fluids. With the control variable method, the effect of the viscosity and surface tension on the bubble growth rate of the RT instability is studied. The result is consistent with Cao＇s result if we only consider the viscous effect and with Xia＇s result if we only consider the surface tension effect. The asymptotic bubble velocity predicted by the Zufiria model is smaller than that predicted by the Layzer model, and the result from the Zufiria model is much closer to White＇s experimental data.展开更多
Background:SIBP04 is a biosimilar of bevacizumab(Avastin■,Roche,Basel,Switzerland).This study evaluated the equivalence of SIBP04 to Avastin■as first-line treatment for locally advanced or metastatic non-squamous no...Background:SIBP04 is a biosimilar of bevacizumab(Avastin■,Roche,Basel,Switzerland).This study evaluated the equivalence of SIBP04 to Avastin■as first-line treatment for locally advanced or metastatic non-squamous non-small-cell lung cancer(nsqNSCLC).Methods:In this randomized,double-blind,multi-center,phase 3 trial,we recruited patients with locally advanced or metastatic nsqNSCLC from 58 hospitals at China.Patients were randomly allocated 1:1 to receive SIBP04 or Avastin■(15 mg/kg)combined with paclitaxel(175 mg/m^(2))and carboplatin(area under curve[AUC]=5.0,no more than 800 mg)(PC)regimens intravenously(3-week cycles,up to six cycles)followed by SIBP04 maintenance therapy.The primary endpoint was objective response rate(ORR),defined as the best overall response from the first dose to the 18th week,assessed by the independent review committee(IRC)according to the Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1.Clinical equivalence of the primary endpoint was done by comparing the two-sided 90%confidence interval(CI)of the ORR ratio(SIBP04 plus PC vs.Avastin■plus PC)in the per-protocol set(PPS)populaiton with the prespecified equivalence margin of 0.75–1.33.Secondary endpoints included progression-free survival,overall survival,duration of response,disease control rate,safety,immunogenicity,and pharmacological bioequivalence of steady-state trough concentrations.Results:From April 17,2020,to April 20,2021,517 patients were randomly assigned to receive SIBP04 plus PC(n=259)or Avastin■plus PC(n=258).The ORR of the SIBP04 plus PC group was 55.6%(95%CI,49.3–61.8)and that of the Avastin■plus PC group was 59.3%(95%CI,53.0–65.4)in the full analysis set(FAS)population(P=0.3944).The ORR of the SIBP04 plus PC group was 62.6%(95%CI,55.8–69.0)and that of the Avastin■plus PC group was 64.7%(95%CI,58.0–71.0)in the PPS population(P=0.6448).The ORR ratio(SIBP04 plus PC vs.Avastin■plus PC)was 0.94(90%CI,0.8270–1.0621)in the FAS population and 0.97(90%CI,0.8578–1.0900)in the PPS population,respectively,both within the prespecified equivalence margin of 0.75–1.33.Other efficacy endpoints,safety,immunogenicity,and pharmacokinetics were all comparable across the groups.Conclusions:SIBP04 showed equivalent efficacy and safety profile to Avastin■in patients with locally advanced or metastatic nsqNSCLC.SIBP04 plus PC regimen will offer an alternative first-line treatment option for this patient population.展开更多
Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product uses.However,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQ...Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product uses.However,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria sp.Numerous‘Huaiju 2#’plants were inoculated with Alternaria sp.for three or five days.Metabolic analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the control.Protein activity analysis also revealed a significant increase in defense enzyme activities in infected plants.RNA-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean reads.Among these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria sp.infection,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling pathways.In particular,SA signaling was highly responsive to Alternaria sp.infection.The qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq data.One candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgenic plants to black spot.These findings indicate that the SA response pathway is likely involved in the defense of‘Huaiju 2#’against Alternaria sp.pathogens.展开更多
Tap water is one of the most commonly used water resources in our daily life. However, the increasing water contamination and the health risk caused by pathogenic bacteria, such as Staphylococcus aureus and Escherichi...Tap water is one of the most commonly used water resources in our daily life. However, the increasing water contamination and the health risk caused by pathogenic bacteria, such as Staphylococcus aureus and Escherichia coli have attracted more attention. The mutualism of different pathogenic bacteria may diminish antibacterial effect of antibacterial agents. It was found that materials used for making pipe and tap played one of the most important roles in promoting bacterial growth. This paper is to report the performance of an innovative type 304 Cu-bearing stainless steel(304Cu SS) against microbes in tap water. The investigation methodologies involved were means of heterotrophic plate count, contact angle measurements, scanning electron microscopy for observing the cell and subtract surface morphology,atomic absorption spectrometry for copper ions release study, and confocal laser scanning microscopy used for examining live/dead bacteria on normal 304 stainless steel and 304 Cu SS. It was found that the surface free energy varied after being immersed in tap water with polar component and Cu ions release.The results showed 304 Cu SS could effectively kill most of the planktonic bacteria(max 95.9% antibacterial rate), and consequently inhibit bacterial biofilms formation on the surface, contributing to the reduction of pathogenic risk to the surrounding environments.展开更多
Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in bi...Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in biomaterials, where copper nanoparticles(Cu NPs) were generated by in situ reductions of Cu ions into a mussel-inspired hyperbranched polyglycerol(MI-h PG) coating via a simple dip-coating method.This hyperbranched polyglycerol with 10 % catechol groups’ modification presents excellent antifouling property, which could effectively reduce bacteria adhesion on the surface. In this work, polycaprolactone(PCL) electrospun fiber membrane was selected as the substrate, which is commonly used in biomedical implants in bone regeneration and cardiovascular stents because of its good biocompatibility and easy post-modification. The as-fabricated Cu NPs-incorporated PCL membrane [PCL-(MI-h PG)-Cu NPs]was confirmed with effective antibacterial performance via in vitro antibacterial tests against Staphylococcus aureus(S. aureus), Escherichia coli(E. coli), and multi-resistant E. coli. In addition, the in vitro results demonstrated that osteogenic property of PCL-(MI-h PG)-Cu NPs was realized by upregulating the osteoblast-related gene expressions and protein activity. This study shows that antibacterial and osteogenic properties can be balanced in a surface coating by introducing Cu NPs.展开更多
In red-fleshed kiwifruit,anthocyanin pigmentation is a crucial commercial trait.The MYB-bHLH-WD40(MBW)complex and other transcription factors regulate its accumulation.Herein,a new SEP gene,AcMADS68,was identified as ...In red-fleshed kiwifruit,anthocyanin pigmentation is a crucial commercial trait.The MYB-bHLH-WD40(MBW)complex and other transcription factors regulate its accumulation.Herein,a new SEP gene,AcMADS68,was identified as a regulatory candidate for anthocyanin biosynthesis in the kiwifruit by transcriptome data and bioinformatic analyses.AcMADS68 alone could not induce the accumulation of anthocyanin both in Actinidia arguta fruit and tobacco leaves.However,in combination with AcMYBF110,AcMYB123,and AcbHLH1,AcMADS68 co-overexpression increased anthocyanin biosynthesis,whereas its silencing reduced anthocyanin accumulation.The results of the dual-luciferase reporter,firefly luciferase complementation,yeast two-hybrid and co-immunoprecipitation assays showed that AcMADS68 could interact with both AcMYBF110 and AcMYB123 but not with AcbHLH1,thereby co-regulating anthocyanin biosynthesis by promoting the activation of the target genes,including AcANS,AcF3GT1,and AcGST1.Moreover,AcMADS68 also could activate the promoter of AcbHLH1 surported by dual-luciferase reporter and yeast one-hybrid assays,thereby further amplifying the regulation signals from the MBW complex,thus resulting in enhanced anthocyanin accumulation in the kiwifruit.These findings may facilitate better elucidation of various regulatory mechanisms underlying anthocyanin accumulation and contribute to the quality enhancement of red-fleshed kiwifruit.展开更多
文摘The study on sand body connectivity and distribution patterns is of great significance for well emplacement and injection-production pattern analysis in the A oilfield of the Weixi?nan Sag currently at a pre-development stage.Based on the current drilling data,seismic data,and fault development characteristics,this study investigates the connectivity,geometric morphology,planar distribution,and vertical evolution of composite sand bodies(multi-stage superimposed channel sand bodies)within the fault block using seismic forward and inversion modeling.The El3I oil layer group in the third member of the Liushagang Formation is developed in the fan delta-front sub-facies,which mainly consists of subaqueous distributary channels.The thickness of single-stage subaqueous distributary channel sand bodies ranges from 2 to 6 m,and the width of composite channel sand bodies varies from 50 to 100 m.Under the long-term transgression background,the subaqueous distributary channels in the El3I oil layer group are relatively narrow,forming superimposed and continuous composite channel sand bodies through lateral migration and vertical stacking.The long-term base-level cycles control the width of subaqueous distributary channels,while the mid-term base-level cycles control the thickness of these channels.The subaqueous distributary channels developed during the late stage of mid-term base-level fall are thicker than those formed during the early stage.Accordingly,quantitative relationships between channel thickness and width are established for the early and late stages of mid-term base-level fall,to finely depict the evolution patterns of channel sand body geometry and stacking styles across different stages.These findings provide important guidance for accurately predicting the planar distribution and channel width of composite subaqueous distributary channels at different stages of the mid-term baselevel cycles.
基金National Defense Basic Scientific Research Program of China(Grant No.JCKY2021602B030).
文摘Multi-axle heavy-duty vehicles(MHVs)are essential for military equipment transport due to their safety and stability.However,braking dynamic responses between MHVs and pavement systems still remain underexplored,particularly regarding their complex load transfer mechanisms.This paper develops an enhanced model of a multi-axle heavy-duty vehicle(MHV)coupled with the uneven and flexible pavement.An advanced coupling iterative method is proposed to solve the highly dimensional equations of the MHV-pavement coupled system.The proposed method was validated through experimental tests,with characteristic parameters of vertical accelerations showing relative errors between 0.42%and 11.80%.The coupling effect and influence mechanism of the braking process are investigated by characteristic parameters of the dynamic responses.Additionally,the influences of braking conditions and pavement parameters are analyzed in time and frequency domains in order to reveal the vibration mechanisms of the coupled system.Moreover,this study establishes a theoretical foundation for monitoring pavement health via vehicle-mounted acceleration signals,which is necessary in military transportation.
基金supported by the National Natural Science Foundation of China(U22B2075)the Fundamental Research Funds for the Central Universities(2024ZKPYSB03)support from Beijing University of Science and Technology.
文摘Tight glutenite reservoirs are known for strong heterogeneity,complex wettability,and challenging development.Gas-Assisted Gravity Drainage(GAGD)technology has the potential to significantly improve recovery efficiency in glutenite reservoir.However,there is currently limited research on GAGD processes specifically designed for glutenite reservoirs,and there is a lack of relevant dimensionless numbers for predicting recovery efficiency.In this study,we developed a theoretical model based on the characteristics of glutenite reservoirs and used phase-field method to track the oil-gas interface for numerical simulations of dynamic GAGD processes.To explore the factors influencing gas-driven recovery,we simulated the effects of strong heterogeneity and dynamic wettability on the construction process under gravity assistance.Additionally,we introduced multiple dimensionless numbers(including capillary number,viscosity ratio,and Bond number)and conducted a series of numerical simulations.The results demonstrate that gravity enhances the stability of the oil-gas interface but causes unstable pressure fluctuations when passing through different-sized throat regions,particularly leading to front advancement in smaller throats.Although strong heterogeneity has negative impacts on GAGD,they can be mitigated by reducing injection velocity.Increasing oil-wettability promotes oil displacement by overcoming capillary forces,particularly in narrower pores,allowing residual oils to be expelled.Among the dimensionless numbers,the recovery efficiency is directly proportional to the Bond number and inversely proportional to the capillary number and viscosity ratio.Through sensitivity analysis of the dimensionless numbers’impact on the recovery efficiency,a new dimensionless N_(Glu) considering heterogeneity is proposed to accurately predict GAGD recovery of tight glutenite reservoirs.
基金supported by the National Natural Science Foundation of China(Grant No.32172521)the Excellent Youth Science Foundation of Heilongjiang Province(Grant No.YQ2023C006)+1 种基金the China Postdoctoral Science Foundation(Grant No.2023MD744175)the Talent Introduction Programof Northeast Agricultural University of China,and the Collaborative Innovation System of the Agricultural Bioeconomy in Heilongjiang Province,China.
文摘The compositions and contents of soluble sugars highly determine the flavor and quality of fleshy fruits.In the present study,we found that the overexpression of transcription factor MdWRKY126 localized on the nucleus enhanced sucrose concentration while decreased fructose and glucose concentration in transgenic apple calli and ripening tomato fruits.To comprehensively understand the effects of the MdWRKY126 on the content of various soluble sugars in apple and tomato fruits,enzyme activities and related essential genes associated with the sugar metabolism and transportation pathway in MdWRKY126-overrexpressed apple and tomato lines were analyzed.The results indicated that the overexpression of MdWRKY126 upregulated sucrose phosphate synthase(SPS)activity and the gene expression levels of SPS and sucrose transporter SUT,which was conducive to a large accumulation of sucrose in fruit cells.Meanwhile,MdWRKY126 overexpression downregulated the activity of enzymes involved in sucrose decomposition including cell wall invertase(CWINV),sucrose synthase(SUSY)and the corresponding gene expressions,as well as inhibited the expression levels of hexose transporter(HTs)and tonoplast sugar transporter(TSTs)that transport hexose into vacuoles,resulting in a reduced hexose level in apple calli and tomato fruit.These findings enrich our understanding of the metabolism and regulation of soluble sugars in apple fruits.
基金This work was supported in part by the National Natural Science Foundation of China(No.31372038 to M.L.)Cornell Agricultural Experiment Station.
文摘Both sorbitol and sucrose are synthesized in source leaves and transported to fruit for supporting fruit growth in tree fruit species of the Rosaceae family.In apple(Malus domestica),antisense suppression of aldose-6-phosphate reductase,the key enzyme for sorbitol synthesis,significantly decreased the sorbitol concentration but increased the sucrose concentration in leaves,leading to a lower sorbitol but a higher sucrose supply to fruit in these plants.In response to this altered carbon supply,the transgenic fruit had lower concentration of sorbitol and much higher concentration of glucose but similar levels of fructose,sucrose,and starch throughout fruit development relative to the untransformed control.Activities of sorbitol dehydrogenase,fructokinase,and sucrose phosphate synthase were lower,whereas activities of neutral invertase,sucrose synthase,and hexokinase were higher in the transgenic fruit during fruit development.Transcript levels of MdSOT1,MdSDHs,MdFK2,and MdSPS3/6 were downregulated,whereas transcript levels of MdSUC1/4,MdSUSY1-3,MdNIV1/3,MdHKs,and MdTMT1 were upregulated in the transgenic fruit.These findings suggest that the Sucrose cycle and the sugar transport system are very effective in maintaining the level of fructose and provide insights into the roles of sorbitol and sucrose in regulating sugar metabolism and accumulation in sorbitol-synthesizing species.
基金financially supported by the National Natural Science Foundation of China(Nos.51101154,51631009,51672184,and 51371168)the National Basic Research Program of China(No.2012CB619101)National Key R&D Program of China(No.2020YFC1107400)。
文摘Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)are the most typical pathogenic bacteria with a significantly high risk of bio-contamination,widely existing in hospital and public places.Recent studies on antibacterial materials and the related mechanisms have attracted more interests of researchers.However,the antibacterial behavior of materials is usually evaluated separately on the single bacterial strain,which is far from the practical condition.Actually,the interaction between the polymicrobial communities can promote the growing profile of bacteria,which may weaken the antibacterial effect of materials.In this work,a 420 copper-bearing martensitic stainless steel(420 CuSS)was studied with respect to its antibacterial activity and the underlying mechanism in a co-culturing infection model using both E.coli and S.au reus.Observed via plating and counting colony forming units(CFU),Cu releasing,and material characterization,420 CuSS was proved to present excellent antibacterial performance against the mixed bacteria with an approximately 99.4%of antibacterial rate.In addition,420 CuSS could effectively inhibit the biofilm formation on its surfaces,resulting from a synergistic antibacterial effect of Cu ions,Fe ions,reactive oxygen species(ROS),and proton consumption of bacteria.
基金This work was supported by the Program for the National Natural Science Foundation of China(No.31372038)by the earmarked fund for the China Agriculture Research System(CARS-28).
文摘To investigate the functions of fructokinase(FRK)in apple(Malus domestica)carbohydrate metabolism,we cloned the coding sequences of MdFRK1 and MdFRK2 from the‘Royal Gala’apple.The results showed that MdFRK2 expression was extremely high in shoot tips and young fruit.Analyses of heterologously expressed proteins revealed that MdFRK2 had a higher affinity for fructose than did MdFRK1,with Km values of 0.1 and 0.62 mM for MdFRK2 and MdFRK1,respectively.The two proteins,however,exhibited similar Vmax values when their activities were significantly inhibited by high concentrations of fructose.MdFRK2 ectopic expression was associated with a general decrease in fructose concentration in transgenic lines.In leaves,increased FRK activity similarly resulted in reduced concentrations of glucose and sucrose but no alterations in sorbitol concentration.When compared with those in the untransformed control,genes involved in sorbitol synthesis(A6PR)and the degradation pathway(SDH1/2)were significantly upregulated in transgenic lines,whereas those involved in sucrose synthesis(SPS1)and other degradation processes(SUSY4,NINV1/2,and HxK2)were downregulated.The activity of enzymes participating in carbohydrate metabolism was proportional to the level of gene expression.However,the growth performance and photosynthetic efficiency did not differ between the transgenic and wild-type plants.These results provide new genetic evidence to support the view that FRK plays roles in regulating sugar and sorbitol metabolism in Rosaceae plants.
基金This work was supported by the Cornell Agricultural Experiment Station and New York Apple Research and Development Program。
文摘Fruits from three cultivars of apple(Malus × domestica Borkh.)—‘McIntosh’,‘Gala’and‘Mutsu’—were harvested from the exterior and interior of the tree canopy.Peel and flesh tissues were sampled separately to determine how the position of the fruit on the tree might affect the levels of the primary and secondary metabolites in the fruit.Fruit from the outer-canopy had a higher fresh weight and a higher soluble solids content compared with inner-canopy fruit.Both the flesh and peel of the outer-canopy fruit had higher concentrations of soluble sugars and sugar alcohols,but lower starch concentrations than the inner-canopy fruit.Canopy position did not significantly affect malic acid concentrations,except in the peel of‘McIntosh’and the flesh of‘Mutsu’.Although levels of ascorbic and succinic acids were higher in the peel of the outer-canopy fruit,the responses of other organic acids to canopy position depended on tissue type and cultivar.Except for histidine,lysine,threonine and glycine,most amino acids accumulated at higher concentrations in the inner-canopy fruit.By contrast,levels of phenolic compounds from both the peel and flesh were significantly higher in the outer-canopy fruit.The significant effects of location within the canopy on both primary metabolites and secondary metabolites demonstrate the importance of light exposure on apple fruit quality.
基金supported by the National Key Research and Development Program of China(No.2016YFD0102106)National Natural Science Foundation of China(Nos.11475103,21627812)。
文摘Cold atmospheric plasmas(CAPs)have shown great applicability in agriculture.Many kinds of CAP sources have been studied in agricultural applications to promote plant growth and cure plant diseases.We briefly review the state-of-the-art stimulating effects of atmospheric-pressure dielectricbarrier-discharge(AP-DBD)plasmas,after the direct or indirect treatment of plants for growth promotion and disease control.We then discuss the special demands on the characteristics of the CAP sources for their applications in plant mutation breeding.An atmospheric and room temperature plasma(ARTP)jet generator with a large plasma irradiation area,a high enough concentration of chemically reactive species and a low gas temperature is designed for direct plant mutagenesis.Experimental measurements of the electrical,thermal and optical features of the ARTP generator are conducted.Then,an ARTP-P(ARTP for plant mutagenesis)mutation breeding machine is developed,and a typical case of plant mutation breeding by the ARTP-P mutation machine is presented using Coreopsis tinctoria Nutt.seeds.Physical and agricultural experiments show that the newly-developed ARTP-P mutation breeding machine with a large irradiation area can generate uniform CAP jets with high concentrations of chemically reactive species and mild gas temperatures,and have signiflcant mutagenesis effects on the Coreopsis tinctoria Nutt.seeds.The ARTP-P mutation breeding machine may provide a platform for systematic studies on mutation mechanisms and results for various plant seeds under different operating conditions in future research.
基金the National Natural Science Foundation of China(No.11971411)the Research Foundation of Education Bureau of Hunan Province of China(No.18A067)。
文摘This study is to numerically test the interfacial instability of ferrofluid flow under the presence of a vacuum magnetic field.The ferrofluid parabolized stability equations(PSEs)are derived from the ferrofluid stability equations and the Rosensweig equations,and the characteristic values of the ferrofluid PSEs are given to describe the ellipticity of ferrofluid flow.Three numerical models representing specific cases considering with/without a vacuum magnetic field or viscosity are created to mathematically examine the interfacial instability by the computation of characteristic values.Numerical investigation shows strong dependence of the basic characteristic of ferrofluid Rayleigh-Taylor instability(RTI)on viscosity of ferrofluid and independence of the vacuum magnetic field.For the shock wave striking helium bubble,the magnetic field is not able to trigger the symmetry breaking of bubble but change the speed of the bubble movement.In the process of droplet formation from a submerged orifice,the collision between the droplet and the liquid surface causes symmetry breaking.Both the viscosity and the magnetic field exacerbate symmetry breaking.The computational results agree with the published experimental results.
基金supported by the National Natural Science Foundation of China(Grant No.32001988)the National Natural Science Foundation of Shaanxi Province(Grant No.2020JC-21)+1 种基金the Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas(Grant No.CSBAA2020002)the earmarked fund for the China Agriculture Research System(Grant No.CARS-27)。
文摘Soluble sugars function not only as the energy and structural blocks supporting plants,but also as osmoregulators and signal molecules during plant adaptation to water deficit.Here,we investigated drought resistance in transgenic apple(Malus×domestica)overexpressing MdFRK2,a key gene regulating fructose content and sugar metabolism.There is no obvious phenotypic difference between MdFRK2-overexpressing transgenic plants and WT plants under the well-watered condition.However,the transgenic plants and the grafted plants using MdFRK2-overexpressing rootstock exhibited improved tolerance to drought stress.Overexpression of MdFRK2 significantly promoted the growth of root system under drought stress.RNA sequencing showed that under drought stress,genes involved in sugar metabolism,transcription regulation,signal transduction or hormone metabolism were differentially expressed in MdFRK2 transgenic plants.Consistent with the gene expression profile,the activities of enzyme(SDH,FRK and NI)involved in sugar metabolism in the roots of MdFRK2 transgenic plants were significantly higher than those of untransformed control plants after drought stress.Under drought stress,overexpression of MdFRK2 promoted the accumulation of IAA,and decreased the contents of ABA and CK in apple root system.In conclusion,these results suggest that MdFRK2 can promote the growth of apple roots under drought stress by regulating sugar metabolism and accumulation,hormone metabolism and signal transduction,and then resist drought stress.
基金Project supported by the National Natural Science Foundation of China(Nos.11171281 and11201389)
文摘Based on the Zufiria theoretical model, a new model regarding the asymptotic bubble velocity for the Rayleigh-Taylor （RT） instability is presented by use of the complex velocity potential proposed by Sohn. The proposed model is an extension of the ordinary Zufiria model and can deal with non-ideal fluids. With the control variable method, the effect of the viscosity and surface tension on the bubble growth rate of the RT instability is studied. The result is consistent with Cao＇s result if we only consider the viscous effect and with Xia＇s result if we only consider the surface tension effect. The asymptotic bubble velocity predicted by the Zufiria model is smaller than that predicted by the Layzer model, and the result from the Zufiria model is much closer to White＇s experimental data.
文摘Background:SIBP04 is a biosimilar of bevacizumab(Avastin■,Roche,Basel,Switzerland).This study evaluated the equivalence of SIBP04 to Avastin■as first-line treatment for locally advanced or metastatic non-squamous non-small-cell lung cancer(nsqNSCLC).Methods:In this randomized,double-blind,multi-center,phase 3 trial,we recruited patients with locally advanced or metastatic nsqNSCLC from 58 hospitals at China.Patients were randomly allocated 1:1 to receive SIBP04 or Avastin■(15 mg/kg)combined with paclitaxel(175 mg/m^(2))and carboplatin(area under curve[AUC]=5.0,no more than 800 mg)(PC)regimens intravenously(3-week cycles,up to six cycles)followed by SIBP04 maintenance therapy.The primary endpoint was objective response rate(ORR),defined as the best overall response from the first dose to the 18th week,assessed by the independent review committee(IRC)according to the Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1.Clinical equivalence of the primary endpoint was done by comparing the two-sided 90%confidence interval(CI)of the ORR ratio(SIBP04 plus PC vs.Avastin■plus PC)in the per-protocol set(PPS)populaiton with the prespecified equivalence margin of 0.75–1.33.Secondary endpoints included progression-free survival,overall survival,duration of response,disease control rate,safety,immunogenicity,and pharmacological bioequivalence of steady-state trough concentrations.Results:From April 17,2020,to April 20,2021,517 patients were randomly assigned to receive SIBP04 plus PC(n=259)or Avastin■plus PC(n=258).The ORR of the SIBP04 plus PC group was 55.6%(95%CI,49.3–61.8)and that of the Avastin■plus PC group was 59.3%(95%CI,53.0–65.4)in the full analysis set(FAS)population(P=0.3944).The ORR of the SIBP04 plus PC group was 62.6%(95%CI,55.8–69.0)and that of the Avastin■plus PC group was 64.7%(95%CI,58.0–71.0)in the PPS population(P=0.6448).The ORR ratio(SIBP04 plus PC vs.Avastin■plus PC)was 0.94(90%CI,0.8270–1.0621)in the FAS population and 0.97(90%CI,0.8578–1.0900)in the PPS population,respectively,both within the prespecified equivalence margin of 0.75–1.33.Other efficacy endpoints,safety,immunogenicity,and pharmacokinetics were all comparable across the groups.Conclusions:SIBP04 showed equivalent efficacy and safety profile to Avastin■in patients with locally advanced or metastatic nsqNSCLC.SIBP04 plus PC regimen will offer an alternative first-line treatment option for this patient population.
基金funded by the Joint Funds of the National Natural Science Foundation of China(No.U1704120)the National Natural Science Foundation of China(No.31372105).
文摘Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product uses.However,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria sp.Numerous‘Huaiju 2#’plants were inoculated with Alternaria sp.for three or five days.Metabolic analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the control.Protein activity analysis also revealed a significant increase in defense enzyme activities in infected plants.RNA-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean reads.Among these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria sp.infection,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling pathways.In particular,SA signaling was highly responsive to Alternaria sp.infection.The qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq data.One candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgenic plants to black spot.These findings indicate that the SA response pathway is likely involved in the defense of‘Huaiju 2#’against Alternaria sp.pathogens.
基金financially supported by the National Natural Science Foundation of China (Nos. 51101154 and 51371168)the National Basic Research Program (No. 2012CB619101)+1 种基金the UK Royal Academy of Engineering (RAEng Ref. 1213RECI052)the Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, and Shenyang National Lab for Materials Science
文摘Tap water is one of the most commonly used water resources in our daily life. However, the increasing water contamination and the health risk caused by pathogenic bacteria, such as Staphylococcus aureus and Escherichia coli have attracted more attention. The mutualism of different pathogenic bacteria may diminish antibacterial effect of antibacterial agents. It was found that materials used for making pipe and tap played one of the most important roles in promoting bacterial growth. This paper is to report the performance of an innovative type 304 Cu-bearing stainless steel(304Cu SS) against microbes in tap water. The investigation methodologies involved were means of heterotrophic plate count, contact angle measurements, scanning electron microscopy for observing the cell and subtract surface morphology,atomic absorption spectrometry for copper ions release study, and confocal laser scanning microscopy used for examining live/dead bacteria on normal 304 stainless steel and 304 Cu SS. It was found that the surface free energy varied after being immersed in tap water with polar component and Cu ions release.The results showed 304 Cu SS could effectively kill most of the planktonic bacteria(max 95.9% antibacterial rate), and consequently inhibit bacterial biofilms formation on the surface, contributing to the reduction of pathogenic risk to the surrounding environments.
基金financially supported by SFB 765 and the Focus Area Nanoscale of Freie Universitat Berlinfinancial support from China Scholarship Councilsupported by the DFG and Dr. Pamela Winchester (Freie Universitat Berlin, Germany) for language polishing。
文摘Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in biomaterials, where copper nanoparticles(Cu NPs) were generated by in situ reductions of Cu ions into a mussel-inspired hyperbranched polyglycerol(MI-h PG) coating via a simple dip-coating method.This hyperbranched polyglycerol with 10 % catechol groups’ modification presents excellent antifouling property, which could effectively reduce bacteria adhesion on the surface. In this work, polycaprolactone(PCL) electrospun fiber membrane was selected as the substrate, which is commonly used in biomedical implants in bone regeneration and cardiovascular stents because of its good biocompatibility and easy post-modification. The as-fabricated Cu NPs-incorporated PCL membrane [PCL-(MI-h PG)-Cu NPs]was confirmed with effective antibacterial performance via in vitro antibacterial tests against Staphylococcus aureus(S. aureus), Escherichia coli(E. coli), and multi-resistant E. coli. In addition, the in vitro results demonstrated that osteogenic property of PCL-(MI-h PG)-Cu NPs was realized by upregulating the osteoblast-related gene expressions and protein activity. This study shows that antibacterial and osteogenic properties can be balanced in a surface coating by introducing Cu NPs.
基金supported by the National Natural Science Foundation of China(72171219,71921001,71801206,71971203)the Fundamental Research Funds for the Central Universities(WK2040000027,WK2040000041)+2 种基金USTC Research Funds of the Double First-Class Initiative(YD2040002004)Special Research Assistant Support Program of Chinese Academy of Sciencesthe Four Batch Talent Programs of China。
基金supported by grants from the National Natural Science Foundation(Grant No.32102312)the modern agricultural industry technology system(Grant No.CARS-26)the National Forestry and Grassland Extension Project(Grant No.K3130219012).
文摘In red-fleshed kiwifruit,anthocyanin pigmentation is a crucial commercial trait.The MYB-bHLH-WD40(MBW)complex and other transcription factors regulate its accumulation.Herein,a new SEP gene,AcMADS68,was identified as a regulatory candidate for anthocyanin biosynthesis in the kiwifruit by transcriptome data and bioinformatic analyses.AcMADS68 alone could not induce the accumulation of anthocyanin both in Actinidia arguta fruit and tobacco leaves.However,in combination with AcMYBF110,AcMYB123,and AcbHLH1,AcMADS68 co-overexpression increased anthocyanin biosynthesis,whereas its silencing reduced anthocyanin accumulation.The results of the dual-luciferase reporter,firefly luciferase complementation,yeast two-hybrid and co-immunoprecipitation assays showed that AcMADS68 could interact with both AcMYBF110 and AcMYB123 but not with AcbHLH1,thereby co-regulating anthocyanin biosynthesis by promoting the activation of the target genes,including AcANS,AcF3GT1,and AcGST1.Moreover,AcMADS68 also could activate the promoter of AcbHLH1 surported by dual-luciferase reporter and yeast one-hybrid assays,thereby further amplifying the regulation signals from the MBW complex,thus resulting in enhanced anthocyanin accumulation in the kiwifruit.These findings may facilitate better elucidation of various regulatory mechanisms underlying anthocyanin accumulation and contribute to the quality enhancement of red-fleshed kiwifruit.
