Background:Many studies have examined the role of genes,proteins,andmicroribonucleic acids(miRNAs)in colorectal cancer(CRC).However,these studies did not establish the regulatory relationships among multi-omics,and on...Background:Many studies have examined the role of genes,proteins,andmicroribonucleic acids(miRNAs)in colorectal cancer(CRC).However,these studies did not establish the regulatory relationships among multi-omics,and only a few have investigated the key genes involved in the transition from colorectal adenoma to CRC.In this study,we established regulatory networks of target gene-miRNA-transcription factors(TFs)to elucidate the pathogenesis of CRC.Methods:Data from 70 patients with CRC were obtained from the Gene Expression Omnibus database.Bioinformatics analyses were used to identify the hub genes involved in the colorectal adenoma-carcinoma sequence.We conducted prognostic evaluations,analyzed gene co-expression patterns,assessed immune cell infiltration,and performed Mendelian randomization.A gene-miRNA-TF network was constructed and further analyzed.Results:Periostin(POSTN),thrombospondin 2(THBS2),collagen alpha-2 type I(COL1A2),and other molecules were found to interact and play key roles in the colorectal adenoma-carcinoma sequence.The 3 genes-11 miRNAs-6 TFs regulatory network we constructed was involved in this process through various pathways and interactions with immune cells.Several molecules in this network affected the final prognosis of patients with CRC.THBS2 showed a causal genetic relationship with neutrophils(p=0.035,odds ratio=1.020[95% confidence interval=1.001-1.039]).Therefore,bleomycin and other drugs may potentially improve the prognosis of patients with CRC.Conclusions:The 3 genes-11 miRNAs-6 TFs regulatory network may provide valuable insights into the pathogenesis of CRC.Additionally,some of these molecules may affect patient prognosis,serving as biomarkers or therapeutic targets.THBS2 may promote neutrophil infiltration into CRC tissues by increasing neutrophil levels in the blood.展开更多
In an era defined by complex,interconnected challenges like climate change,pandemics,and resource depletion,the traditional siloed approach to science education is proving increasingly insufficient.Interdisciplinary p...In an era defined by complex,interconnected challenges like climate change,pandemics,and resource depletion,the traditional siloed approach to science education is proving increasingly insufficient.Interdisciplinary project-based learning represents a promising path forward in science education,fostering integrated and holistic learning experiences that move beyond isolated subject learning.Grounded in philosophical ideas of holism,pragmatism,constructivism,and transcendentalism,this article presents a case project illustrating the practical application of interdisciplinary project-based learning.This project engages students in integrating concepts from biology,chemistry,earth science,engineering,and social studies.Through phased activities-research and planning,data collection,implementation,and presentation-students develop a decent understanding of real-world problems while fostering skills in collaboration,problem-solving,and a sense of civic responsibility.Additionally,strategies are proposed to navigate the challenges associated with implementing interdisciplinary project-based learning,including aligning projects with standards,investing in professional development,leveraging community resources,and building support from stakeholders.展开更多
Ti6Al4V3Cu alloy is a promising biomaterial for combating implant-related infection.However,the antibacterial property of Ti6Al4V3Cu is expected to be enhanced due to the low content of Cu element in titanium.To addre...Ti6Al4V3Cu alloy is a promising biomaterial for combating implant-related infection.However,the antibacterial property of Ti6Al4V3Cu is expected to be enhanced due to the low content of Cu element in titanium.To address this issue,the antibacterial property of Ti6Al4V3Cu was tailored by the grooves with different groove widths(30μm,60μm,and 90μm)that were constructed on the surface of the Ti6Al4V3Cu by selective laser melting.The effect of grooves on corrosion resistance,antibacterial property,and cytocompatibility was investigated.The electrochemical tests showed that the corrosion resistance decreased with increasing groove width.The antibacterial tests indicated that the groove with a width of 30μm and 90μm groups showed better antibacterial activity against S.aureus(>90%)compared with the groove with a width of 60μm.The in vitro study suggested that all samples with different grooves were found to exhibit good cytocompatibility with osteoblast cells.It is considered that creating grooves on Ti6Al4V3Cu by selective laser melting is a promising strategy to enhance the antibacterial activity without sacrificing cytocompatibility.展开更多
To summarize the nursing experience of a three-stage liver resection combined with ALPPS(associating liver partition and portal vein ligation for staged hepatectomy)and intraoperative isolated perfusion chemotherapy f...To summarize the nursing experience of a three-stage liver resection combined with ALPPS(associating liver partition and portal vein ligation for staged hepatectomy)and intraoperative isolated perfusion chemotherapy for a patient at a tertiary hospital in Shenzhen,China,in order to provide insights for the treatment of such cases in the future.This operation was conducted for the first time globally,presenting significant challenges and no prior nursing experience to reference.Through evidence-based thinking and literature review,preoperative multidisciplinary team(MDT)collaboration was implemented for the patient.This included psychological support,rehabilitation planning,preoperative exercise regimens,comprehensive nutritional interventions,standardized pain management,and close monitoring of the patient’s blood drug concentrations during the operation.Intraoperatively,prevention and observation of potential complications were prioritized.As a result of the MDT interventions,the patient did not experience any related complications or adverse events during the perioperative period and was successfully discharged after recovery.Follow-up examinations conducted one month,six months,and one year post-discharge revealed no tumor recurrence,with all outcomes being favorable.展开更多
It is preliminary estimated that the proved geological reserves of original low-oil-saturation reservoirs(OLOSRs)of 54 blocks in China are more than 820 million tons.As same as the high water-cut stage of a reservoir ...It is preliminary estimated that the proved geological reserves of original low-oil-saturation reservoirs(OLOSRs)of 54 blocks in China are more than 820 million tons.As same as the high water-cut stage of a reservoir after water flooding,the initial water cut of OLOSRS is higher than 80%.The industrial application of EOR technology could efficiently enhance oil recovery rate from 8.4% to 20.1% of reservoirs after water flooding.The suitable measurements of EOR to the OLOSRs probably have a profound impact on achieving higher oilfield reserves and production.In this paper,the main characteristics and reserves potential of OLOSRs have been studied by investigations and laboratory experiments.The relationship between initial oil saturation and displacement efficiency is also analyzed.The feasibility of EOR technologies for different OLOSRs is discussed.The results show that the OLOSRs could be divided into two categories(primary and secondary)by origin of low oil saturation.The two categories contain abundant reserves in China’s major oilfields,but their occurrence states of crude oil are quite different.If the average oil saturation of the OLOSR is defined at 40%,there are 13.1%-54.2%crude oil that could be recovered by using the existing EOR technology,especially in the primary OLOSRs with low permeability and the secondary OLOSRs with high permeability.展开更多
文摘Background:Many studies have examined the role of genes,proteins,andmicroribonucleic acids(miRNAs)in colorectal cancer(CRC).However,these studies did not establish the regulatory relationships among multi-omics,and only a few have investigated the key genes involved in the transition from colorectal adenoma to CRC.In this study,we established regulatory networks of target gene-miRNA-transcription factors(TFs)to elucidate the pathogenesis of CRC.Methods:Data from 70 patients with CRC were obtained from the Gene Expression Omnibus database.Bioinformatics analyses were used to identify the hub genes involved in the colorectal adenoma-carcinoma sequence.We conducted prognostic evaluations,analyzed gene co-expression patterns,assessed immune cell infiltration,and performed Mendelian randomization.A gene-miRNA-TF network was constructed and further analyzed.Results:Periostin(POSTN),thrombospondin 2(THBS2),collagen alpha-2 type I(COL1A2),and other molecules were found to interact and play key roles in the colorectal adenoma-carcinoma sequence.The 3 genes-11 miRNAs-6 TFs regulatory network we constructed was involved in this process through various pathways and interactions with immune cells.Several molecules in this network affected the final prognosis of patients with CRC.THBS2 showed a causal genetic relationship with neutrophils(p=0.035,odds ratio=1.020[95% confidence interval=1.001-1.039]).Therefore,bleomycin and other drugs may potentially improve the prognosis of patients with CRC.Conclusions:The 3 genes-11 miRNAs-6 TFs regulatory network may provide valuable insights into the pathogenesis of CRC.Additionally,some of these molecules may affect patient prognosis,serving as biomarkers or therapeutic targets.THBS2 may promote neutrophil infiltration into CRC tissues by increasing neutrophil levels in the blood.
基金supported by the Anhui Provincial Education Science Research Program titled“Research on the Construction and Application of Evaluation Frameworks for Interdisciplinary Practical Activities in Primary School Science”(JKT25114)the Humanities and Social Sciences Research Program of Anhui Higher Education Institutions(2022AH052117).
文摘In an era defined by complex,interconnected challenges like climate change,pandemics,and resource depletion,the traditional siloed approach to science education is proving increasingly insufficient.Interdisciplinary project-based learning represents a promising path forward in science education,fostering integrated and holistic learning experiences that move beyond isolated subject learning.Grounded in philosophical ideas of holism,pragmatism,constructivism,and transcendentalism,this article presents a case project illustrating the practical application of interdisciplinary project-based learning.This project engages students in integrating concepts from biology,chemistry,earth science,engineering,and social studies.Through phased activities-research and planning,data collection,implementation,and presentation-students develop a decent understanding of real-world problems while fostering skills in collaboration,problem-solving,and a sense of civic responsibility.Additionally,strategies are proposed to navigate the challenges associated with implementing interdisciplinary project-based learning,including aligning projects with standards,investing in professional development,leveraging community resources,and building support from stakeholders.
基金the study and collection,analysis,and interpretation of data were supported by the National Natural Science Foundation of China(No.51801198)the Funds of Scientific and Technological Plan of Fujian Province(Nos.2020Y9064,2020Y0083 and 2020L3026)Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China(No.2021ZZ111).
文摘Ti6Al4V3Cu alloy is a promising biomaterial for combating implant-related infection.However,the antibacterial property of Ti6Al4V3Cu is expected to be enhanced due to the low content of Cu element in titanium.To address this issue,the antibacterial property of Ti6Al4V3Cu was tailored by the grooves with different groove widths(30μm,60μm,and 90μm)that were constructed on the surface of the Ti6Al4V3Cu by selective laser melting.The effect of grooves on corrosion resistance,antibacterial property,and cytocompatibility was investigated.The electrochemical tests showed that the corrosion resistance decreased with increasing groove width.The antibacterial tests indicated that the groove with a width of 30μm and 90μm groups showed better antibacterial activity against S.aureus(>90%)compared with the groove with a width of 60μm.The in vitro study suggested that all samples with different grooves were found to exhibit good cytocompatibility with osteoblast cells.It is considered that creating grooves on Ti6Al4V3Cu by selective laser melting is a promising strategy to enhance the antibacterial activity without sacrificing cytocompatibility.
文摘To summarize the nursing experience of a three-stage liver resection combined with ALPPS(associating liver partition and portal vein ligation for staged hepatectomy)and intraoperative isolated perfusion chemotherapy for a patient at a tertiary hospital in Shenzhen,China,in order to provide insights for the treatment of such cases in the future.This operation was conducted for the first time globally,presenting significant challenges and no prior nursing experience to reference.Through evidence-based thinking and literature review,preoperative multidisciplinary team(MDT)collaboration was implemented for the patient.This included psychological support,rehabilitation planning,preoperative exercise regimens,comprehensive nutritional interventions,standardized pain management,and close monitoring of the patient’s blood drug concentrations during the operation.Intraoperatively,prevention and observation of potential complications were prioritized.As a result of the MDT interventions,the patient did not experience any related complications or adverse events during the perioperative period and was successfully discharged after recovery.Follow-up examinations conducted one month,six months,and one year post-discharge revealed no tumor recurrence,with all outcomes being favorable.
基金supported by the CNPC program of New Technologies and Methods for Oil and Gas Field Development(2016A-0903).
文摘It is preliminary estimated that the proved geological reserves of original low-oil-saturation reservoirs(OLOSRs)of 54 blocks in China are more than 820 million tons.As same as the high water-cut stage of a reservoir after water flooding,the initial water cut of OLOSRS is higher than 80%.The industrial application of EOR technology could efficiently enhance oil recovery rate from 8.4% to 20.1% of reservoirs after water flooding.The suitable measurements of EOR to the OLOSRs probably have a profound impact on achieving higher oilfield reserves and production.In this paper,the main characteristics and reserves potential of OLOSRs have been studied by investigations and laboratory experiments.The relationship between initial oil saturation and displacement efficiency is also analyzed.The feasibility of EOR technologies for different OLOSRs is discussed.The results show that the OLOSRs could be divided into two categories(primary and secondary)by origin of low oil saturation.The two categories contain abundant reserves in China’s major oilfields,but their occurrence states of crude oil are quite different.If the average oil saturation of the OLOSR is defined at 40%,there are 13.1%-54.2%crude oil that could be recovered by using the existing EOR technology,especially in the primary OLOSRs with low permeability and the secondary OLOSRs with high permeability.
