Polyurethane is an excellent and widely used polymer material.In synthesizing polyurethane,the structure of soft and hard segments in polyurethane could be adjusted,which can obtain better properties than other polyme...Polyurethane is an excellent and widely used polymer material.In synthesizing polyurethane,the structure of soft and hard segments in polyurethane could be adjusted,which can obtain better properties than other polymer materials,such as good mechanical properties and biocompatibility.In recent years,due to their excellent chemical stability,biocompatibility,and low cytotoxicity,polyurethanes have been widely used for biomedical applications.To solve the problems of degradation and recycling,the development of bio-based polyurethane has also become a current hot spot.This paper summarizes the research progress and applications of polyurethane materials for dressings,skin sensors,orthopedics,and cardiovascular.It also looks forward to the future development of polyurethane medical materials.展开更多
Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in Eleutherococcus senticosus.The results of bisulfite sequencing showed that there were three kinds of samples with a ...Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in Eleutherococcus senticosus.The results of bisulfite sequencing showed that there were three kinds of samples with a low(0.68%),medium(0.72%)and high(0.79%)DNA methylation ratio in the promoter of MDD in E.senticosus,respectively.The transcriptome sequencing results showed that the expression of MDD in E.senticosus was significantly up-regulated in the types with low DNA methylation ratios of MDD(P<0.05).There was a significant negative correlation between the saponin content in E.senticosus and the DNA methylation ratio of MDD promoter(P<0.05).The screening results of differentially expressed transcription factors among the three groups with different DNA methylation ratio showed that a total of 4 transcription factors could bind to 6 CpG sites.Protein-protein interaction analysis showed that E.senticosus MDD could interacted with other key enzymes in the process of terpenoid synthesis.In addition,it was found that the DNA methylation of MDD promoter was mainly regulated by DNA methyltransferase.These results demonstrated that under the action of DNA methyltransferase,the changes of DNA methylation of MDD promoter could regulate its own expression level by affecting the combination ability of transcription factors,and then affected the expression of other genes in terpenoid synthesis pathway.The synthesis and accumulation of saponins in E.senticosus was also changed.展开更多
Post-traumatic stress disorder(PTSD)is a relatively common but complex mental illness with a range of diverse risk factors.Typical symptoms include the re-experience or avoidance of traumatic events,cognitive impairme...Post-traumatic stress disorder(PTSD)is a relatively common but complex mental illness with a range of diverse risk factors.Typical symptoms include the re-experience or avoidance of traumatic events,cognitive impairment,and hypervigilance.While the exact pathogenesis of PTSD is unclear,many studies indicate that epigenetic regulation plays a key role in its development.Specifically,numerous studies have indicated that the levels of histone acetylation and methylation,DNA methylation,and noncoding RNA are altered in PTSD patients.Further to this,natural products have been found to achieve epigenetic regulation of PTSD by regulating the expression of epigenetic enzymes,long noncoding RNA(lncRNA),and miRNA,thereby playing a role in improving PTSD symptoms.To date,however,no epigenetic regulation related drugs have been used in the treatment of PTSD.Furthermore,while natural products that can epigenetically regulate PTSD have received increasing levels of attention,there have not yet been any systematic reports on the topic.Here,we summarized the roles and mechanisms of natural products in the epigenetic regulation of PTSD,providing a novel and unique perspective that will help to guide the development and application of new PTSD treatments.展开更多
Kagome lattice,characterized by two-dimensional honeycomb network of corner-sharing triangles[1],presents flat bands,Dirac cones,and van Hove singularities(VHSs),which have been theoretically predicted and experimenta...Kagome lattice,characterized by two-dimensional honeycomb network of corner-sharing triangles[1],presents flat bands,Dirac cones,and van Hove singularities(VHSs),which have been theoretically predicted and experimentally observed[2-4].When combined with spin-orbit coupling(SOC)and magnetism,novel properties have emerged.Although kagome materials vary,most of their strong interlayer interactions make the synthesized crystals not layered,and the properties deviating from the raw two-dimensional kagome lattices.These crystals are difficult to fabricate into thin devices and to tune the physical properties of the materials using gate voltage.展开更多
Cognitive impairment caused by chronic cerebral hypoperfusion(CCH)is associated with white matter injury(WMI),possibly through the alteration of autophagy.Here,the autophagy—lysosomal pathway(ALP)dysfunction in white...Cognitive impairment caused by chronic cerebral hypoperfusion(CCH)is associated with white matter injury(WMI),possibly through the alteration of autophagy.Here,the autophagy—lysosomal pathway(ALP)dysfunction in white matter(WM)and its relationship with cognitive impairment were investigated in rats subjected to two vessel occlusion(2VO).The results showed that cognitive impairment occurred by the 28th day after 2VO.Injury and autophagy activation of mature oligodendrocytes and neuronal axons sequentially occurred in WM by the 3rd day.By the 14th day,abnormal accumulation of autophagy substrate,lysosomal dysfunction,and the activation of mechanistic target of rapamycin(MTOR)pathway were observed in WM,paralleled with mature oligodendrocyte death.This indicates autophagy activation was followed by ALP dysfunction caused by autophagy inhibition or lysosomal dysfunction.To target the ALP dysfunction,enhanced autophagy by systemic rapamycin treatment or overexpression of Beclin1(BECN1)in oligodendrocytes reduced mature oligodendrocyte death,and subsequently alleviated the WMI and cognitive impairment after CCH.These results reveal that early autophagy activation was followed by ALP dysfunction in WM after 2VO,which was associated with the aggravation of WMI and cognitive impairment.This study highlights that alleviating ALP dysfunction by enhancing oligodendrocyte autophagy has benefits for cognitive recovery after CCH.展开更多
Metabolite biosynthesis is regulated by gene expression,which is altered by DNA methylation in the promoter region.Chalcone isomerase(CHI)gene encodes a key enzyme in the Lithocarpus polystachyus Rehd flavonoid pathwa...Metabolite biosynthesis is regulated by gene expression,which is altered by DNA methylation in the promoter region.Chalcone isomerase(CHI)gene encodes a key enzyme in the Lithocarpus polystachyus Rehd flavonoid pathway,and the expression of L.polystachyus CHI(LpCHI)is closely related to the synthesis of flavonoid metabolites.In this study,we analyzed the DNA methylation site of the LpCHI promoter and its effect on gene expression and metabolite accumulation.The proportions of three types of LpCHI promoter DNA methylation are 7.5%,68.75%,18.75%,determined by bisulfite sequencing.Transcriptome sequencing shows that LpCHI is strongly up-regulated in LpCHI promoter methylation Type A but down-regulated in LpCHI promoter methylation Type B and Type C.The expression of LpCHI shows no significant difference between Type B and Type C.Moreover,nine kinds of differentially expressed transcription factors(DETFs)bind to seven CpG-sites of the LpCHI promoter region to regulate LpCHI expression.The results of metabolomics show that differentially accumulated flavonoids are higher in LpCHI promoter methylation Type A than in LpCHI promoter methylation Type B and Type C.Additionally,a positive correlation was found between the LpCHI expression and flavonoids accumulation.These results show that the effect of CpG site-specificity on gene transcription is great than that of overall promoter DNA methylation on gene transcription.The mechanisms of flavonoid genes regulating metabolite accumulation are further revealed.展开更多
基金Financial support from National Key Research and Development Program of China(2017YFE0102300)S&T Innovation 2025 Major Special Program of Ningbo(2022Z139)Scientific Research Funding Project of the Educational Department of Liaoning Province(LQ2020008)。
文摘Polyurethane is an excellent and widely used polymer material.In synthesizing polyurethane,the structure of soft and hard segments in polyurethane could be adjusted,which can obtain better properties than other polymer materials,such as good mechanical properties and biocompatibility.In recent years,due to their excellent chemical stability,biocompatibility,and low cytotoxicity,polyurethanes have been widely used for biomedical applications.To solve the problems of degradation and recycling,the development of bio-based polyurethane has also become a current hot spot.This paper summarizes the research progress and applications of polyurethane materials for dressings,skin sensors,orthopedics,and cardiovascular.It also looks forward to the future development of polyurethane medical materials.
基金funded by Natural Science Foundation of Hebei Province,Grant No.H2020209302 and Hebei Education Department Sponsored Scientific Research Projects,Grant No.ZD2019075.
文摘Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in Eleutherococcus senticosus.The results of bisulfite sequencing showed that there were three kinds of samples with a low(0.68%),medium(0.72%)and high(0.79%)DNA methylation ratio in the promoter of MDD in E.senticosus,respectively.The transcriptome sequencing results showed that the expression of MDD in E.senticosus was significantly up-regulated in the types with low DNA methylation ratios of MDD(P<0.05).There was a significant negative correlation between the saponin content in E.senticosus and the DNA methylation ratio of MDD promoter(P<0.05).The screening results of differentially expressed transcription factors among the three groups with different DNA methylation ratio showed that a total of 4 transcription factors could bind to 6 CpG sites.Protein-protein interaction analysis showed that E.senticosus MDD could interacted with other key enzymes in the process of terpenoid synthesis.In addition,it was found that the DNA methylation of MDD promoter was mainly regulated by DNA methyltransferase.These results demonstrated that under the action of DNA methyltransferase,the changes of DNA methylation of MDD promoter could regulate its own expression level by affecting the combination ability of transcription factors,and then affected the expression of other genes in terpenoid synthesis pathway.The synthesis and accumulation of saponins in E.senticosus was also changed.
基金supported by the Project of National Natural Science Foundation of China(No.82074041,82004051,81803508)Funded by China Postdoctoral Science Foundation(No.2020M670674)+4 种基金the Scientific Research Fund from Liaoning Education Department(No.2020LQN02,LJKZ0923,LJKQZ2021029)Natural Science Foundation of Liaoning Province(No.2021-MS-216)Fund for distinguished young scholars of Shenyang Pharmaceutical University(No.ZQ202202)Fund for outstanding young scholars of Shenyang Pharmaceutical University(No.YQ202103)rolling funding of young and middle-aged teachers'career development project of Shenyang Pharmaceutical University。
文摘Post-traumatic stress disorder(PTSD)is a relatively common but complex mental illness with a range of diverse risk factors.Typical symptoms include the re-experience or avoidance of traumatic events,cognitive impairment,and hypervigilance.While the exact pathogenesis of PTSD is unclear,many studies indicate that epigenetic regulation plays a key role in its development.Specifically,numerous studies have indicated that the levels of histone acetylation and methylation,DNA methylation,and noncoding RNA are altered in PTSD patients.Further to this,natural products have been found to achieve epigenetic regulation of PTSD by regulating the expression of epigenetic enzymes,long noncoding RNA(lncRNA),and miRNA,thereby playing a role in improving PTSD symptoms.To date,however,no epigenetic regulation related drugs have been used in the treatment of PTSD.Furthermore,while natural products that can epigenetically regulate PTSD have received increasing levels of attention,there have not yet been any systematic reports on the topic.Here,we summarized the roles and mechanisms of natural products in the epigenetic regulation of PTSD,providing a novel and unique perspective that will help to guide the development and application of new PTSD treatments.
基金National Key Research and Development Program of China(2022YFA1402404)National Natural Science Foundation of China(92161201,T2221003,12104221,12104220,12274208,12025404,12004174,91961101,61822403,11874203,and 12374043)+1 种基金Natural Science Foundation of Jiangsu Province(BK20230079)Fundamental Research Funds for the Central Universities(020414380192 and 2024300432)。
文摘Kagome lattice,characterized by two-dimensional honeycomb network of corner-sharing triangles[1],presents flat bands,Dirac cones,and van Hove singularities(VHSs),which have been theoretically predicted and experimentally observed[2-4].When combined with spin-orbit coupling(SOC)and magnetism,novel properties have emerged.Although kagome materials vary,most of their strong interlayer interactions make the synthesized crystals not layered,and the properties deviating from the raw two-dimensional kagome lattices.These crystals are difficult to fabricate into thin devices and to tune the physical properties of the materials using gate voltage.
基金the Natural Science Foundation of Liaoning Province(LJKQZ2021031,2022-MS-246,China)to Yueyang Liu。
文摘Cognitive impairment caused by chronic cerebral hypoperfusion(CCH)is associated with white matter injury(WMI),possibly through the alteration of autophagy.Here,the autophagy—lysosomal pathway(ALP)dysfunction in white matter(WM)and its relationship with cognitive impairment were investigated in rats subjected to two vessel occlusion(2VO).The results showed that cognitive impairment occurred by the 28th day after 2VO.Injury and autophagy activation of mature oligodendrocytes and neuronal axons sequentially occurred in WM by the 3rd day.By the 14th day,abnormal accumulation of autophagy substrate,lysosomal dysfunction,and the activation of mechanistic target of rapamycin(MTOR)pathway were observed in WM,paralleled with mature oligodendrocyte death.This indicates autophagy activation was followed by ALP dysfunction caused by autophagy inhibition or lysosomal dysfunction.To target the ALP dysfunction,enhanced autophagy by systemic rapamycin treatment or overexpression of Beclin1(BECN1)in oligodendrocytes reduced mature oligodendrocyte death,and subsequently alleviated the WMI and cognitive impairment after CCH.These results reveal that early autophagy activation was followed by ALP dysfunction in WM after 2VO,which was associated with the aggravation of WMI and cognitive impairment.This study highlights that alleviating ALP dysfunction by enhancing oligodendrocyte autophagy has benefits for cognitive recovery after CCH.
基金supported by the Natural Science Foundation of Hebei Province(H2020209033)the Science and Technology Project of Hebei Education Department(ZD2019075).
文摘Metabolite biosynthesis is regulated by gene expression,which is altered by DNA methylation in the promoter region.Chalcone isomerase(CHI)gene encodes a key enzyme in the Lithocarpus polystachyus Rehd flavonoid pathway,and the expression of L.polystachyus CHI(LpCHI)is closely related to the synthesis of flavonoid metabolites.In this study,we analyzed the DNA methylation site of the LpCHI promoter and its effect on gene expression and metabolite accumulation.The proportions of three types of LpCHI promoter DNA methylation are 7.5%,68.75%,18.75%,determined by bisulfite sequencing.Transcriptome sequencing shows that LpCHI is strongly up-regulated in LpCHI promoter methylation Type A but down-regulated in LpCHI promoter methylation Type B and Type C.The expression of LpCHI shows no significant difference between Type B and Type C.Moreover,nine kinds of differentially expressed transcription factors(DETFs)bind to seven CpG-sites of the LpCHI promoter region to regulate LpCHI expression.The results of metabolomics show that differentially accumulated flavonoids are higher in LpCHI promoter methylation Type A than in LpCHI promoter methylation Type B and Type C.Additionally,a positive correlation was found between the LpCHI expression and flavonoids accumulation.These results show that the effect of CpG site-specificity on gene transcription is great than that of overall promoter DNA methylation on gene transcription.The mechanisms of flavonoid genes regulating metabolite accumulation are further revealed.
