This study was conducted to explore the effects of polycyclic aromatic hydrocarbons (PAHs) on the growth and physiological characteristics of vegetable seedlings.Cucumber,Chinese cabbage and radish were cultured under...This study was conducted to explore the effects of polycyclic aromatic hydrocarbons (PAHs) on the growth and physiological characteristics of vegetable seedlings.Cucumber,Chinese cabbage and radish were cultured under the stress of 16 PAHs at different concentrations of 0.3,0.6 and 0.9 mg/L,respectively,with the seedlings cultured without PAHs as the control,to study the growth and physiological responses of various vegetable seedlings under PAHs stress.The results showed that the leaf width,fresh weight,CAT activity and POD activity of cucumber and the chlorophyll b content and CAT activity of radish reached the minimum values when treated with 0.3 mg/L PAHs.When the treatment concentration was 0.6 mg/L,the POD activity of cucumber,the vitamin C content of Chinese cabbage and the MDA content of radish were the smallest.The dry weight of cucumber,the fresh weight of Chinese cabbage and the fresh weight and POD activity of radish reached the minimums under the treatment of 0.9 mg/L PAHs.Different concentrations of PAHs could promote the synthesis and photosynthesis of chlorophyll in cucumber and Chinese cabbage leaves and the vitamin C content in radish.With the increase of PAHs treatment concentration,the MDA contents of cucumber and Chinese cabbage were on the increase,and reached the maximums under the treatment of 0.9 mg/L PAHs.One of the reasons why different kinds of vegetable seedlings have different physiological responses to PAHs may be related to the differences in the amount of PAHs absorbed by different leaf structures.展开更多
[Objective]This study aimed to investigate various factors affecting the dedifferentiation of different floral organs of Jinxiu wild chive.[Method]Floral organs of Jinxiu wild chive were cultured in vitro,to analyze t...[Objective]This study aimed to investigate various factors affecting the dedifferentiation of different floral organs of Jinxiu wild chive.[Method]Floral organs of Jinxiu wild chive were cultured in vitro,to analyze the effects of basic medium,exogenous hormone type and combination,and blossoming status of buds on callus induction.[Result]MS medium was the most suitable medium for callus induction from buds.NAA was conducive to improving callus formation rate compared with other auxins.Non-blossomed buds exhibited the highest rate of callus induction.Under the same culture conditions,the rate of callus induction varied extremely significantly among various floral organs;to be specific,the rate of callus induction from floral disc reached the highest,followed successively by bud and pedicel,but no callus was formed in floral axis group.[Conclusion]This study laid the foundation for further establishment of tissue culture and rapid propagation system of Jinxiu wild chive and other wild chives and provided technical support for the preservation,development and utilization of wild chive germplasm resources.展开更多
基金Supported by National Natural Science Foundation of China(31360479)National Modern Agriculture Technology System of Guangxi in Special Vegetable Innovation Team(nycytxgxcxtd-10-03)Natural Science Foundation of Guangxi Province(2014GXNSFAA118100)
文摘This study was conducted to explore the effects of polycyclic aromatic hydrocarbons (PAHs) on the growth and physiological characteristics of vegetable seedlings.Cucumber,Chinese cabbage and radish were cultured under the stress of 16 PAHs at different concentrations of 0.3,0.6 and 0.9 mg/L,respectively,with the seedlings cultured without PAHs as the control,to study the growth and physiological responses of various vegetable seedlings under PAHs stress.The results showed that the leaf width,fresh weight,CAT activity and POD activity of cucumber and the chlorophyll b content and CAT activity of radish reached the minimum values when treated with 0.3 mg/L PAHs.When the treatment concentration was 0.6 mg/L,the POD activity of cucumber,the vitamin C content of Chinese cabbage and the MDA content of radish were the smallest.The dry weight of cucumber,the fresh weight of Chinese cabbage and the fresh weight and POD activity of radish reached the minimums under the treatment of 0.9 mg/L PAHs.Different concentrations of PAHs could promote the synthesis and photosynthesis of chlorophyll in cucumber and Chinese cabbage leaves and the vitamin C content in radish.With the increase of PAHs treatment concentration,the MDA contents of cucumber and Chinese cabbage were on the increase,and reached the maximums under the treatment of 0.9 mg/L PAHs.One of the reasons why different kinds of vegetable seedlings have different physiological responses to PAHs may be related to the differences in the amount of PAHs absorbed by different leaf structures.
基金Supported by Special Fund for Bulk Vegetable Innovation Team of Guangxi Zhuang Autonomous Region from National Modern Agriculture Industry Technology System(nycytxgxcxtd-03-10-1)Natural Science Foundation of Guangxi Zhuang Autonomous Region(2011GXNSFB018033)+2 种基金Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(201001)Key Project of Science and Technology of Guangxi Zhuang Autonomous Region(GKG 0323001-1)Project of Nanning Bureau of Science and Technology(201102057B)
文摘[Objective]This study aimed to investigate various factors affecting the dedifferentiation of different floral organs of Jinxiu wild chive.[Method]Floral organs of Jinxiu wild chive were cultured in vitro,to analyze the effects of basic medium,exogenous hormone type and combination,and blossoming status of buds on callus induction.[Result]MS medium was the most suitable medium for callus induction from buds.NAA was conducive to improving callus formation rate compared with other auxins.Non-blossomed buds exhibited the highest rate of callus induction.Under the same culture conditions,the rate of callus induction varied extremely significantly among various floral organs;to be specific,the rate of callus induction from floral disc reached the highest,followed successively by bud and pedicel,but no callus was formed in floral axis group.[Conclusion]This study laid the foundation for further establishment of tissue culture and rapid propagation system of Jinxiu wild chive and other wild chives and provided technical support for the preservation,development and utilization of wild chive germplasm resources.
