SP600125 is an inhibitor of c-Jun NH2-terminal kinase (JNK), which plays a fundamental role in regulating animal development. Using SP600125 to deal with the mouse embryonic stem cells, it is revealed that the number ...SP600125 is an inhibitor of c-Jun NH2-terminal kinase (JNK), which plays a fundamental role in regulating animal development. Using SP600125 to deal with the mouse embryonic stem cells, it is revealed that the number of the ESC colonies decreased and the size became smaller. With treatment by SP600125, the proliferation of mouse ES cells is seriously inhibited for the cycle arrested in the G2/M phase, and the effect of SP600125 on the ES cells displays correlation of dose and time. The obtained results indicate that JNK may be an important regulator in the progression of cell cycle at the G2/M cell phase for the ES cells.展开更多
The laboratory rat was one of the earliest mammalian species for scientific research and used as animal disease models in physiology, toxicology, behavior, immunology, and tumor-biology for over 150 years （Jacob, 199...The laboratory rat was one of the earliest mammalian species for scientific research and used as animal disease models in physiology, toxicology, behavior, immunology, and tumor-biology for over 150 years （Jacob, 1999）. However, rat lags far behind mouse in generating human disease models and functional genomic studies because of the lack of authentic rat embryonic stem （ES） cells （Voigt and Serikawa, 2009）,展开更多
文摘SP600125 is an inhibitor of c-Jun NH2-terminal kinase (JNK), which plays a fundamental role in regulating animal development. Using SP600125 to deal with the mouse embryonic stem cells, it is revealed that the number of the ESC colonies decreased and the size became smaller. With treatment by SP600125, the proliferation of mouse ES cells is seriously inhibited for the cycle arrested in the G2/M phase, and the effect of SP600125 on the ES cells displays correlation of dose and time. The obtained results indicate that JNK may be an important regulator in the progression of cell cycle at the G2/M cell phase for the ES cells.
基金supported by the grants from the China National Basic Research Program(No.2012CB966501) to X.Zthe National High Technology R&D Program of China (No.2011AA020108) to Q.Z.the "Strategic Priority Research Program" of the Chinese Academy of Sciences(No. XDA01030101)
文摘The laboratory rat was one of the earliest mammalian species for scientific research and used as animal disease models in physiology, toxicology, behavior, immunology, and tumor-biology for over 150 years （Jacob, 1999）. However, rat lags far behind mouse in generating human disease models and functional genomic studies because of the lack of authentic rat embryonic stem （ES） cells （Voigt and Serikawa, 2009）,
