Alternaria alternata is a prevalent pathogenic fungus that infects a wide variety of fruits and vegetables,resulting in substantial economic losses.Microbial volatile organic compounds(mVOCs)serve as effective biofumi...Alternaria alternata is a prevalent pathogenic fungus that infects a wide variety of fruits and vegetables,resulting in substantial economic losses.Microbial volatile organic compounds(mVOCs)serve as effective biofumigants for the control of fungal infestations.In this study,we evaluated the inhibitory activity of volatile compounds produced(VOCs)emitted by Saccharomycopsis fibuligera KN-1 against A.alternata,demonstrating its capability to effectively inhibit mycelial growth and retard spore germination.Headspace solid-phase microextraction-gas chromatography/mass spectrometry(HS-SPME-GC/MS)was used to identify 23 volatile organic compounds,mainly including alcohols,acids,esters,phenols,ethers and alkanes.Among them,2-methylbutyric acid plays an important role in the antifungal effect.Phenylethanol and 3-methyl-1-butanol also demonstrated strong inhibitory effects.Notably,combining the IC_(50) values of ethyl octanoate and 2-methylbutyric acid resulted in significant synergistic effects.Furthermore,SEM analysis revealed that the abnormal morphology of A.alternata mycelium was observed after fumigation.In addition,the cell membrane may be a legitimate target of the VOCs by S.fibuligera KN-1.Transcriptomic and proteomic analyses indicated that S.fibuligera KN-1 disrupts the interconversion of pentose and gluconate,oxidative phosphorylation,and ABC transporters.This disruption impairs ATP production and carbon utilization,resulting in mitochondrial damage,decreased stress resistance,suggesting potential mechanistic interconnections.Consequently,S.fibuligera KN-1 emerges as a promising antifungal agent A.alternata infections.展开更多
基金supported by Shandong Province Key R&D Plan(Rural Revitalization Science and Technology Innovation Boosting Action Plan)Project(2024TZXD012,2023TZXD056)2021 Taishan Industry Leading Talent Project(tscx202211079)the special Funds of the Central Government Guiding Local Science and Technology Development(YDZX2023098).
文摘Alternaria alternata is a prevalent pathogenic fungus that infects a wide variety of fruits and vegetables,resulting in substantial economic losses.Microbial volatile organic compounds(mVOCs)serve as effective biofumigants for the control of fungal infestations.In this study,we evaluated the inhibitory activity of volatile compounds produced(VOCs)emitted by Saccharomycopsis fibuligera KN-1 against A.alternata,demonstrating its capability to effectively inhibit mycelial growth and retard spore germination.Headspace solid-phase microextraction-gas chromatography/mass spectrometry(HS-SPME-GC/MS)was used to identify 23 volatile organic compounds,mainly including alcohols,acids,esters,phenols,ethers and alkanes.Among them,2-methylbutyric acid plays an important role in the antifungal effect.Phenylethanol and 3-methyl-1-butanol also demonstrated strong inhibitory effects.Notably,combining the IC_(50) values of ethyl octanoate and 2-methylbutyric acid resulted in significant synergistic effects.Furthermore,SEM analysis revealed that the abnormal morphology of A.alternata mycelium was observed after fumigation.In addition,the cell membrane may be a legitimate target of the VOCs by S.fibuligera KN-1.Transcriptomic and proteomic analyses indicated that S.fibuligera KN-1 disrupts the interconversion of pentose and gluconate,oxidative phosphorylation,and ABC transporters.This disruption impairs ATP production and carbon utilization,resulting in mitochondrial damage,decreased stress resistance,suggesting potential mechanistic interconnections.Consequently,S.fibuligera KN-1 emerges as a promising antifungal agent A.alternata infections.
