Phosphatase and tensin homolog deleted on chromosome 10（PTEN） and the proliferating antigen Ki67 have been widely studied in several tumors.However,their role as indicator in non-small cell lung cancer（NSCLC）remai...Phosphatase and tensin homolog deleted on chromosome 10（PTEN） and the proliferating antigen Ki67 have been widely studied in several tumors.However,their role as indicator in non-small cell lung cancer（NSCLC）remains unknown.Here,we investigated the expression of PTEN and Ki67 in NSCLC tissues and paired normal lung tissues to identify whether these proteins are associated with lung cancer development and survival.Immunohistochemistry for PTEN and Ki67 was performed on 67 lung cancer tissues and 41 paired adjacent normal lung tissues to detect the expression of these two proteins.The expression of PTEN in NSCLC tissues（32.8%） was significantly lower than that in normal tissues（82.9%,P 〈 0.05）.In contrast,the expression of Ki67 in NSCLC tissues（76.1%） was significantly higher than that in normal tissues（27.3%,P 〈 0.05）.Expression of both PTEN and Ki67 were strongly associated with tumor histology,clinical stage,lymph node metastasis,differentiation and4-year postoperative survival rate（P 〈 0.05）.However,PTEN expression was negatively correlated with Ki67 expression（r =-0.279,P 〈 0.05）.In conclusion,low PTEN expression and Ki67 overexpression are associated with malignant invasion and lymph node metastasis of NSCLC.These proteins may serve as diagnostic and prognostic biomarkers of NSCLC.展开更多
This study explored the therapeutic effects of Auricularia auricula melanin(AAM)on alcoholic liver damage in vitro and in vivo.Human normal liver L02 cells were pre-treated with ethanol and then treated with AAM to ex...This study explored the therapeutic effects of Auricularia auricula melanin(AAM)on alcoholic liver damage in vitro and in vivo.Human normal liver L02 cells were pre-treated with ethanol and then treated with AAM to explore the therapeutic effect of AAM on ethanol-induced hepatocyte injury.The results show that AAM signifi cantly elevated the cell viability,ameliorated the cell morphology,reduced the ROS and increased the GSH/GSSG of ethanol-pretreated L02 cells.Then,mice were administered with ethanol to induce acute alcoholic liver damage,and administered with AAM to further study the therapeutic effect of AAM on alcoholic liver damage in mice.As a result,AAM reduced the levels of ALT,AST,TG,and MDA,increased the levels of ADH,SOD,and CAT in liver damage mice.The therapeutic effect of AAM may be related to inhibition of CYP2E1 expression and activation of Nrf2 and its downstream antioxidase.The research enriched the bioactivity of AAM and provided some ideas for the development of melanin-related health foods.展开更多
Tremella fuciformis polysaccharides have rich bioactivities,while low molecular weight polysaccharides have higher bioavailability and nutritional value.In this study,it was investigated that the structural characteri...Tremella fuciformis polysaccharides have rich bioactivities,while low molecular weight polysaccharides have higher bioavailability and nutritional value.In this study,it was investigated that the structural characteristics of low molecular weight polysaccharides from Tremella fuciformis(TFLP)prepared by acid hydrolysis method,its impact on antioxidant capacity and stress resistance of Caenorhabditis elegans,and potential mechanisms.TFLP was an acidic polysaccharide mainly composed of mannose and xylose with a molecular weight of 106 kDa.TFLP could prolong the median lifespan of C.elegans under heat stress and acute oxidative stress conditions through significantly reducing the malondialdehyde(MDA)level and increasing the activity of antioxidant enzymes total-superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)and catalase(CAT).The intervention of high-dose TFLP significantly prolonged the median lifespan of C.elegans under heat stress and acute oxidative stress conditions by 24.47%and 7.84%,respectively.At the same time,the MDA level significantly decreased by 69.59%,and the levels of T-SOD,GSH-Px and CAT increased significantly by 2.22,1.28 and 0.53 times,respectively.Meanwhile,the transcription levels of daf-16,fat-5,fat-7,and hsf-1 mRNA in C.elegans treated with TFLP were significantly increased,while the transcription levels of akt-1 and daf-2 mRNA were significantly reduced.In addition,propanoate metabolism,valine,leucine and isoleucine degradation metabolism and vitamin B6 metabolism were key metabolic pathways for TFLP to enhance antioxidant capacity and stress resistance in C.elegans.These results indicated that TFLP could enhance in vivo antioxidant capacity and stress resistance by improving the levels mRNA transcription and metabolites,and it provided new evidence for TFLP to exert in vivo antioxidant activity.展开更多
Identifying the maturity of asparagus is a crucial step for machine-assisted harvesting of asparagus in complex environments.This study proposes an innovative method to evaluate the height and diameter of asparagus st...Identifying the maturity of asparagus is a crucial step for machine-assisted harvesting of asparagus in complex environments.This study proposes an innovative method to evaluate the height and diameter of asparagus stems,combining an enhanced YOLOv5 detection algorithm with point cloud data.In this method,first,the YOLOv5 model was improved,enabling efficient recognition and detection of asparagus in complex environments.Subsequently,a RealSense L515 radar camera was deployed to capture both the original RGB images and the point cloud information.The improved YOLOv5 algorithm was then employed to detect asparagus instances within the RGB images,with the pixel positions of the detection frames mapped onto the point cloud dataset to extract comprehensive 3D point cloud details of the asparagus.Finally,noise was reduced through statistical filtering and Euclidean clustering,and asparagus height was determined using the oriented bounding box methodology.Slices,each with a thickness of 10 mm,were extracted at designated measurement points,and the asparagus diameter was calculated by assessing the disparity between the maximum and minimum coordinates perpendicular to the growth direction of the asparagus.Experimental results showed that the mean average precision,precision,and recall of the improved YOLOv5 model increased by 4.85%,5.09%,and 3.4%,reaching 98.21%,97.11%,and 95.33%,respectively,which are higher than those of the YOLOv5 prototype network.Therefore,the proposed method could effectively detect asparagus.The algorithm exhibited a mean absolute error of 1.08 cm,a mean absolute percentage error of 4.06%,and a root mean square error of 1.60 cm in its estimation of asparagus height.For asparagus diameter estimation,the algorithm achieved a mean absolute error of 0.86 mm,a mean absolute percentage error of 7.98%,and a root mean square error of 1.23 mm.These results confirm that the proposed method can estimate the height and diameter of asparagus stems accurately,thereby providing invaluable technical support for machine harvesting of asparagus.展开更多
Immune checkpoint inhibitors(ICI)targeting PD-1/PD-L1 have been approved for the treatment of a variety of cancers.However,the efficacy of antibody-based ICIs could be further improved by mitigating anti-drug antibodi...Immune checkpoint inhibitors(ICI)targeting PD-1/PD-L1 have been approved for the treatment of a variety of cancers.However,the efficacy of antibody-based ICIs could be further improved by mitigating anti-drug antibodies,proteolytic cleavage,and on-target off-tumor toxicity.One strategy for accomplishing this is through the use of extracellular vesicles(EVs),cell derived submicron vesicles with many unique properties.We constructed an engineered MDA-MB-231 cell line for harvesting EVs.This was accomplished by overexpressing a high-affinity variant human PD-1 protein(havPD-1),while simultaneously knocking out intrinsic PD-L1 and beta-2 microglobulin.The engineered havPD-1 EVs reduced PD-L1 overexpressing cancer cell proliferation and induced cellular apoptosis.Moreover,the EVs were shown to efficiently block PD-L1 mediated T cell suppression.Meanwhile antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity were not observed.The havPD-1 EVs treatment resulted in robust anti-tumor activity in both preventative co-implantation and therapeutic xenograft tumor models reconstituted with human T cells.The efficacy of the havPD-1 EVs was shown to be comparable to clinical anti-PD1 monoclonal antibodies.Additionally,loading the havPD-1 EVs with a potent PARP inhibitor was shown to further augment treatment efficacy.In brief,the engineered universal EVs harboring havPD-1 proteins can be used for cancer concurrent immunotherapy and chemotherapy.展开更多
基金supported by Nanjing Medical University Focus Development and Natural Science Foundation of China
文摘Phosphatase and tensin homolog deleted on chromosome 10（PTEN） and the proliferating antigen Ki67 have been widely studied in several tumors.However,their role as indicator in non-small cell lung cancer（NSCLC）remains unknown.Here,we investigated the expression of PTEN and Ki67 in NSCLC tissues and paired normal lung tissues to identify whether these proteins are associated with lung cancer development and survival.Immunohistochemistry for PTEN and Ki67 was performed on 67 lung cancer tissues and 41 paired adjacent normal lung tissues to detect the expression of these two proteins.The expression of PTEN in NSCLC tissues（32.8%） was significantly lower than that in normal tissues（82.9%,P 〈 0.05）.In contrast,the expression of Ki67 in NSCLC tissues（76.1%） was significantly higher than that in normal tissues（27.3%,P 〈 0.05）.Expression of both PTEN and Ki67 were strongly associated with tumor histology,clinical stage,lymph node metastasis,differentiation and4-year postoperative survival rate（P 〈 0.05）.However,PTEN expression was negatively correlated with Ki67 expression（r =-0.279,P 〈 0.05）.In conclusion,low PTEN expression and Ki67 overexpression are associated with malignant invasion and lymph node metastasis of NSCLC.These proteins may serve as diagnostic and prognostic biomarkers of NSCLC.
基金This work was financially supported by the Special Fund Project for Technological Innovation of Fujian Agriculture and Forestry University(CXZX2019055G)the Science and Technology Project on Social Development of Cixi(CN2020027).
文摘This study explored the therapeutic effects of Auricularia auricula melanin(AAM)on alcoholic liver damage in vitro and in vivo.Human normal liver L02 cells were pre-treated with ethanol and then treated with AAM to explore the therapeutic effect of AAM on ethanol-induced hepatocyte injury.The results show that AAM signifi cantly elevated the cell viability,ameliorated the cell morphology,reduced the ROS and increased the GSH/GSSG of ethanol-pretreated L02 cells.Then,mice were administered with ethanol to induce acute alcoholic liver damage,and administered with AAM to further study the therapeutic effect of AAM on alcoholic liver damage in mice.As a result,AAM reduced the levels of ALT,AST,TG,and MDA,increased the levels of ADH,SOD,and CAT in liver damage mice.The therapeutic effect of AAM may be related to inhibition of CYP2E1 expression and activation of Nrf2 and its downstream antioxidase.The research enriched the bioactivity of AAM and provided some ideas for the development of melanin-related health foods.
基金funded by Science and Technology Planning Project of Fujian Province(2021L3007).
文摘Tremella fuciformis polysaccharides have rich bioactivities,while low molecular weight polysaccharides have higher bioavailability and nutritional value.In this study,it was investigated that the structural characteristics of low molecular weight polysaccharides from Tremella fuciformis(TFLP)prepared by acid hydrolysis method,its impact on antioxidant capacity and stress resistance of Caenorhabditis elegans,and potential mechanisms.TFLP was an acidic polysaccharide mainly composed of mannose and xylose with a molecular weight of 106 kDa.TFLP could prolong the median lifespan of C.elegans under heat stress and acute oxidative stress conditions through significantly reducing the malondialdehyde(MDA)level and increasing the activity of antioxidant enzymes total-superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)and catalase(CAT).The intervention of high-dose TFLP significantly prolonged the median lifespan of C.elegans under heat stress and acute oxidative stress conditions by 24.47%and 7.84%,respectively.At the same time,the MDA level significantly decreased by 69.59%,and the levels of T-SOD,GSH-Px and CAT increased significantly by 2.22,1.28 and 0.53 times,respectively.Meanwhile,the transcription levels of daf-16,fat-5,fat-7,and hsf-1 mRNA in C.elegans treated with TFLP were significantly increased,while the transcription levels of akt-1 and daf-2 mRNA were significantly reduced.In addition,propanoate metabolism,valine,leucine and isoleucine degradation metabolism and vitamin B6 metabolism were key metabolic pathways for TFLP to enhance antioxidant capacity and stress resistance in C.elegans.These results indicated that TFLP could enhance in vivo antioxidant capacity and stress resistance by improving the levels mRNA transcription and metabolites,and it provided new evidence for TFLP to exert in vivo antioxidant activity.
基金support provided by the National Natural Science Foundation of China(Grant No.32171899).
文摘Identifying the maturity of asparagus is a crucial step for machine-assisted harvesting of asparagus in complex environments.This study proposes an innovative method to evaluate the height and diameter of asparagus stems,combining an enhanced YOLOv5 detection algorithm with point cloud data.In this method,first,the YOLOv5 model was improved,enabling efficient recognition and detection of asparagus in complex environments.Subsequently,a RealSense L515 radar camera was deployed to capture both the original RGB images and the point cloud information.The improved YOLOv5 algorithm was then employed to detect asparagus instances within the RGB images,with the pixel positions of the detection frames mapped onto the point cloud dataset to extract comprehensive 3D point cloud details of the asparagus.Finally,noise was reduced through statistical filtering and Euclidean clustering,and asparagus height was determined using the oriented bounding box methodology.Slices,each with a thickness of 10 mm,were extracted at designated measurement points,and the asparagus diameter was calculated by assessing the disparity between the maximum and minimum coordinates perpendicular to the growth direction of the asparagus.Experimental results showed that the mean average precision,precision,and recall of the improved YOLOv5 model increased by 4.85%,5.09%,and 3.4%,reaching 98.21%,97.11%,and 95.33%,respectively,which are higher than those of the YOLOv5 prototype network.Therefore,the proposed method could effectively detect asparagus.The algorithm exhibited a mean absolute error of 1.08 cm,a mean absolute percentage error of 4.06%,and a root mean square error of 1.60 cm in its estimation of asparagus height.For asparagus diameter estimation,the algorithm achieved a mean absolute error of 0.86 mm,a mean absolute percentage error of 7.98%,and a root mean square error of 1.23 mm.These results confirm that the proposed method can estimate the height and diameter of asparagus stems accurately,thereby providing invaluable technical support for machine harvesting of asparagus.
基金Thanks to Dr.L.Nathan Tumey for proofreading the article and suggestions.The work was partially supported by National Cancer Institute(1R01CA230339 subaward and 1R01CA255948)Jiangsu Provincial Medical Youth Talent Award(QNRC2016054)+3 种基金Nanjing Medical Science and Technology Development Foundation Major Program(ZDX16008)Precision Medicine Project of Wuxi Municipal Commission of Health and Family Planning(J201805)the Youth scientific research project of Wuxi municipal health commission(Q201951)and Top Talent Support Program for young and middle-aged people of Wuxi Health Committee(HB2020003).
文摘Immune checkpoint inhibitors(ICI)targeting PD-1/PD-L1 have been approved for the treatment of a variety of cancers.However,the efficacy of antibody-based ICIs could be further improved by mitigating anti-drug antibodies,proteolytic cleavage,and on-target off-tumor toxicity.One strategy for accomplishing this is through the use of extracellular vesicles(EVs),cell derived submicron vesicles with many unique properties.We constructed an engineered MDA-MB-231 cell line for harvesting EVs.This was accomplished by overexpressing a high-affinity variant human PD-1 protein(havPD-1),while simultaneously knocking out intrinsic PD-L1 and beta-2 microglobulin.The engineered havPD-1 EVs reduced PD-L1 overexpressing cancer cell proliferation and induced cellular apoptosis.Moreover,the EVs were shown to efficiently block PD-L1 mediated T cell suppression.Meanwhile antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity were not observed.The havPD-1 EVs treatment resulted in robust anti-tumor activity in both preventative co-implantation and therapeutic xenograft tumor models reconstituted with human T cells.The efficacy of the havPD-1 EVs was shown to be comparable to clinical anti-PD1 monoclonal antibodies.Additionally,loading the havPD-1 EVs with a potent PARP inhibitor was shown to further augment treatment efficacy.In brief,the engineered universal EVs harboring havPD-1 proteins can be used for cancer concurrent immunotherapy and chemotherapy.
