Complex genetic architecture is the major cause of heterogeneity in epilepsy,which poses challenges for accurate diagnosis and precise treatment.A large number of epilepsy candidate genes have been identified from cli...Complex genetic architecture is the major cause of heterogeneity in epilepsy,which poses challenges for accurate diagnosis and precise treatment.A large number of epilepsy candidate genes have been identified from clinical studies,particularly with the widespread use of next-generation sequencing.Validating these candidate genes is emerging as a valuable yet challenging task.Drosophila serves as an ideal animal model for validating candidate genes associated with neurogenetic disorders such as epilepsy,due to its rapid reproduction rate,powerful genetic tools,and efficient use of ethological and electrophysiological assays.Here,we systematically summarize the advantageous techniques of the Drosophila model used to investigate epilepsy genes,including genetic tools for manipulating target gene expression,ethological assays for seizure-like behaviors,electrophysiological techniques,and functional imaging for recording neural activity.We then introduce several typical strategies for identifying epilepsy genes and provide new insights into gene-gene interactions in epilepsy with polygenic causes.We summarize well-established precision medicine strategies for epilepsy and discuss prospective treatment options,including drug therapy and gene therapy for genetic epilepsy based on the Drosophila model.Finally,we also address genetic counseling and assisted reproductive technology as potential approaches for the prevention of genetic epilepsy.展开更多
The current study aimed to compare the effects between remimazolam and propofol on hemodynamic stability during the induction of general anesthesia in elderly patients.We used propofol at a rate of 60 mg/(kg·h)in...The current study aimed to compare the effects between remimazolam and propofol on hemodynamic stability during the induction of general anesthesia in elderly patients.We used propofol at a rate of 60 mg/(kg·h)in the propofol group(group P)or remimazolam at a rate of 6 mg/(kg·h)in the remimazolam group(group R)for the induction.A processed electroencephalogram was used to determine whether the induction was successful and when to stop the infusion of the study drug.We measured when patients entered the operating room(T_(0)),when the induction was successful(T_(1)),and when before(T_(2))and 5 min after successful endotracheal intubation(T_(3)).We found that mean arterial pressure(MAP)was lower at T_(1–3),compared with T_(0) in both groups,but higher at T_(2) in the group R,whileΔMAP_(T0–T2) andΔMAP_(max) were smaller in the group R(ΔMAP_(T0–T2):the difference between MAP at time point T_(0) and T_(2),ΔMAP_(max):the difference between MAP at time point T_(0) and the lowest value from T_(0) to T_(3)).Cardiac index and stroke volume index did not differ between groups,whereas systemic vascular resistance index was higher at T_(1–3) in the group R.These findings show that remimazolam,compared with propofol,better maintains hemodynamic stability during the induction,which may be attributed to its ability to better maintain systemic vascular resistance levels.展开更多
BACKGROUND： Previously, time data were analyzed by using constituent ratio or relative ratio; however, circular statistical analysis could exactly provide average peak phase of diseases. OBJECTIVE： To investigate th...BACKGROUND： Previously, time data were analyzed by using constituent ratio or relative ratio; however, circular statistical analysis could exactly provide average peak phase of diseases. OBJECTIVE： To investigate the correlation of solar term peak with onset and death of acute ischemic stroke. DESIGN： Retrospective case analysis. SETTINGS： Emergency Department of Foshan Municipal Hospital of Traditional Chinese Medicine; Department of Science and Education, the Second People＇s Hospital of Foshan. PARTICIPANTS： A total of 1 597 patients with acute ischemic stroke were selected from Emergency Room, Department of Neurology, Foshan Municipal Hospital of Traditional Chinese Medicine from 1994 to 2002. There were 875 males and 722 females, and their ages ranged from 33 to 97 years. All cases met the diagnostic criteria of acute cerebral infarction modified by the Second National Cerebrovascular Disease Academic Meeting; meanwhile, they were diagnosed with CT/MRI test. Patients and their relatives provided the confirmed consent. METHODS： Solar term of onset was retrospectively analyzed in 1 597 patients with acute ischemic stroke; among them, solar term of death in 90 cases were analyzed by using circular statistical analysis to calculate peak phase of onset and death of acute ischemic stroke and investigate the correlation of solar term with onset and death of acute ischemic stroke. MAIN OUTCOME MEASURES： Onset and death time of patients with acute ischemic stroke. RESULTS： Solar term of onset in 1 597 patients, especially solar term of death in 90 patients, was not concentrated （P 〉 0.05）, and specific peak phase was not found out. Acute ischemic stroke low attacked from vernal equinox to summer begins, but death caused by acute ischemic stroke high attacked from grain buds to autumn begins. CONCLUSION： Patients with acute ischemic stroke do not have specific solar term peak of onset and death.展开更多
The characteristic of individual is described by the Penna model. Based oninformation entropy and the Penna model we define the entropy in the Penna modelto discuss common ancestors and genetic diversity for two repro...The characteristic of individual is described by the Penna model. Based oninformation entropy and the Penna model we define the entropy in the Penna modelto discuss common ancestors and genetic diversity for two reproduction modes withand without mutation. About the problem of common ancestor, we find that all livingindividuals at any time step have a set of common ancestors which belongs to differenttimes. They have the most recent common ancestor and earlier common ancestors.The analysis of genetic diversity shows that the complexity of ecosystems is caused bymutations and there is no contribution of sexual reproduction to conserving geneticdiversity at long time scales. Moreover, in stable environment genetic diversity inasexual reproduction mode is higher than that in the sexual case.展开更多
We report resuscitation of a near-drowning child by a bystander in a rural south China township.Resuscitation was performed on a 6-year-old,otherwise healthy boy who was witnessed drowning in a river,with submersion f...We report resuscitation of a near-drowning child by a bystander in a rural south China township.Resuscitation was performed on a 6-year-old,otherwise healthy boy who was witnessed drowning in a river,with submersion for approximately 12 min.Following several resuscitation attempts,spontaneous breathing was established at the scene and the boy was transferred to a nearby hospital.He developed pneumonia but recovered completely on the ward and was discharged without any neurologic disabilities.This case shows that early bystander resuscitation is an important factor for survival after submersion.The search for an ideal approach to resuscitation must continue,and all members of society must work together towards this goal.展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation,No.2022A1515111123(to JQ)。
文摘Complex genetic architecture is the major cause of heterogeneity in epilepsy,which poses challenges for accurate diagnosis and precise treatment.A large number of epilepsy candidate genes have been identified from clinical studies,particularly with the widespread use of next-generation sequencing.Validating these candidate genes is emerging as a valuable yet challenging task.Drosophila serves as an ideal animal model for validating candidate genes associated with neurogenetic disorders such as epilepsy,due to its rapid reproduction rate,powerful genetic tools,and efficient use of ethological and electrophysiological assays.Here,we systematically summarize the advantageous techniques of the Drosophila model used to investigate epilepsy genes,including genetic tools for manipulating target gene expression,ethological assays for seizure-like behaviors,electrophysiological techniques,and functional imaging for recording neural activity.We then introduce several typical strategies for identifying epilepsy genes and provide new insights into gene-gene interactions in epilepsy with polygenic causes.We summarize well-established precision medicine strategies for epilepsy and discuss prospective treatment options,including drug therapy and gene therapy for genetic epilepsy based on the Drosophila model.Finally,we also address genetic counseling and assisted reproductive technology as potential approaches for the prevention of genetic epilepsy.
文摘The current study aimed to compare the effects between remimazolam and propofol on hemodynamic stability during the induction of general anesthesia in elderly patients.We used propofol at a rate of 60 mg/(kg·h)in the propofol group(group P)or remimazolam at a rate of 6 mg/(kg·h)in the remimazolam group(group R)for the induction.A processed electroencephalogram was used to determine whether the induction was successful and when to stop the infusion of the study drug.We measured when patients entered the operating room(T_(0)),when the induction was successful(T_(1)),and when before(T_(2))and 5 min after successful endotracheal intubation(T_(3)).We found that mean arterial pressure(MAP)was lower at T_(1–3),compared with T_(0) in both groups,but higher at T_(2) in the group R,whileΔMAP_(T0–T2) andΔMAP_(max) were smaller in the group R(ΔMAP_(T0–T2):the difference between MAP at time point T_(0) and T_(2),ΔMAP_(max):the difference between MAP at time point T_(0) and the lowest value from T_(0) to T_(3)).Cardiac index and stroke volume index did not differ between groups,whereas systemic vascular resistance index was higher at T_(1–3) in the group R.These findings show that remimazolam,compared with propofol,better maintains hemodynamic stability during the induction,which may be attributed to its ability to better maintain systemic vascular resistance levels.
基金the grants from Guangdong Province Administration of Traditional Chinese Medicine, No.401007
文摘BACKGROUND： Previously, time data were analyzed by using constituent ratio or relative ratio; however, circular statistical analysis could exactly provide average peak phase of diseases. OBJECTIVE： To investigate the correlation of solar term peak with onset and death of acute ischemic stroke. DESIGN： Retrospective case analysis. SETTINGS： Emergency Department of Foshan Municipal Hospital of Traditional Chinese Medicine; Department of Science and Education, the Second People＇s Hospital of Foshan. PARTICIPANTS： A total of 1 597 patients with acute ischemic stroke were selected from Emergency Room, Department of Neurology, Foshan Municipal Hospital of Traditional Chinese Medicine from 1994 to 2002. There were 875 males and 722 females, and their ages ranged from 33 to 97 years. All cases met the diagnostic criteria of acute cerebral infarction modified by the Second National Cerebrovascular Disease Academic Meeting; meanwhile, they were diagnosed with CT/MRI test. Patients and their relatives provided the confirmed consent. METHODS： Solar term of onset was retrospectively analyzed in 1 597 patients with acute ischemic stroke; among them, solar term of death in 90 cases were analyzed by using circular statistical analysis to calculate peak phase of onset and death of acute ischemic stroke and investigate the correlation of solar term with onset and death of acute ischemic stroke. MAIN OUTCOME MEASURES： Onset and death time of patients with acute ischemic stroke. RESULTS： Solar term of onset in 1 597 patients, especially solar term of death in 90 patients, was not concentrated （P 〉 0.05）, and specific peak phase was not found out. Acute ischemic stroke low attacked from vernal equinox to summer begins, but death caused by acute ischemic stroke high attacked from grain buds to autumn begins. CONCLUSION： Patients with acute ischemic stroke do not have specific solar term peak of onset and death.
文摘The characteristic of individual is described by the Penna model. Based oninformation entropy and the Penna model we define the entropy in the Penna modelto discuss common ancestors and genetic diversity for two reproduction modes withand without mutation. About the problem of common ancestor, we find that all livingindividuals at any time step have a set of common ancestors which belongs to differenttimes. They have the most recent common ancestor and earlier common ancestors.The analysis of genetic diversity shows that the complexity of ecosystems is caused bymutations and there is no contribution of sexual reproduction to conserving geneticdiversity at long time scales. Moreover, in stable environment genetic diversity inasexual reproduction mode is higher than that in the sexual case.
文摘We report resuscitation of a near-drowning child by a bystander in a rural south China township.Resuscitation was performed on a 6-year-old,otherwise healthy boy who was witnessed drowning in a river,with submersion for approximately 12 min.Following several resuscitation attempts,spontaneous breathing was established at the scene and the boy was transferred to a nearby hospital.He developed pneumonia but recovered completely on the ward and was discharged without any neurologic disabilities.This case shows that early bystander resuscitation is an important factor for survival after submersion.The search for an ideal approach to resuscitation must continue,and all members of society must work together towards this goal.
