Herein,a novel label-free electrochemical immunosensor was fabricated via immobilizing specific anti-β-lactoglobulin(β-LG)antibodies(Abs)onto an integrated electrode of gold nanoparticles(AuNPs)/Prussian blue(PB)/cu...Herein,a novel label-free electrochemical immunosensor was fabricated via immobilizing specific anti-β-lactoglobulin(β-LG)antibodies(Abs)onto an integrated electrode of gold nanoparticles(AuNPs)/Prussian blue(PB)/cubic Ia3d structured mesoporous carbon(CMK-8).This immunosensor allowed for the quantitative detection of the major milk allergenβ-LG.CMK-8 with excellent electrical conductivity and uniformly adjustable pore structure was modified on the glassy carbon electrode(GCE)and served as the sensitive substrate for the electro-polymerization of PB,forming the redox-active layer.AuNPs were subsequently electrochemically deposited on PB/CMK-8/GCE to improve the electrical conductivity and utilized as the connector for Abs immobilization.Duringβ-LG detection,the Abs-modified AuNPs/PB/CMK-8/GCE exhibited a significant reduction in differential pulse voltammetry current signal when exposed toβ-LG,displaying an inverse dose-dependent relationship.The developed electrochemical immunosensor demonstrated good detection performance forβ-LG,with a wider linear range of 0.01-100 ng/mL and a lower detection limit of 4.72 pg/mL.Meanwhile,the sensor exhibited remarkable repeatability,reproducibility,stability and anti-interference capabilities,which was further applied to detectβ-LG in dairy food,achieving satisfactory recoveries(89.2%-98.8%)and lower relative standard deviation(£3.1%).Therefore,this innovative electrochemical method for food allergen detection holds great potential application in food safety determination and evaluation.展开更多
In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk products.Experimental ...In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk products.Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment,amount of anti-β-LG antibody and regeneration reagent were optimized in detail.Under optimal conditions,the proposed QCM immunochip exhibited good recognition of β-LG,with a calibration curve of ΔF=12.877 C_(β-LG)^(0.4809)(R^(2)=0.9982)and limit of detection of 0.04μg/mL.Additionally,this portable QCM immunochip had good stability,high specificity,and no obvious cross-reaction to three other milk proteins(α-casein,α-lactalbumin,and lactoferrin).It could compete a qualitative measurement within5 min,and could be reused at least ten times.In the β-LG analysis of actual milk samples,the developed QCM immunochip yielded reliable and accurate results,which correlated strongly with those from the standard HPLC method(R^(2)=0.9969).Thus,the portable,stable,and reproducible QCM immunochip developed in this study allowed the rapid,cost-effectively and sensitively measure theβ-LG in milk products.展开更多
Superhydrophobic coatings that physically separate metal substrates from aqueous media have emerged as a promising strategy against metal corrosion;however,their practical application is hindered by poor mechanical du...Superhydrophobic coatings that physically separate metal substrates from aqueous media have emerged as a promising strategy against metal corrosion;however,their practical application is hindered by poor mechanical durability and rapid performance degradation in the harsh environment.Herein,inspired by the granular architecture and dynamic metal coordination chemistry in mussel byssus cuticle,a hierarchical metal coordination-mediated self-adaptive coating(SC)integrating surface superhydrophobicity,self-healing anticorrosion,and damage-monitoring capacity is constructed on steel substrates using the metal-organic framework(MOF)as the multifunctional nanoplatform.Specifically,a MOF-polydopamine nanocomposite coating is fabricated on mild steel via a coordination-dissociation-polymerization mechanism,where the MOF serves as a self-sacrificial template to initiate the deposition of polydopamine,and the SC is obtained after the subsequent hydrophobization via Michael addition and Schiff base reaction.The super-hydrophobic surface of SC with a water contact angle of 160°provides a superior passive barrier against corrosive media,showing a protective efficiency of 97.5%.Furthermore,the MOF-polydopamine interlayer endows the SC with superior corrosion-triggered self-healing properties by forming protective adsorption films at the exposed steel surface,thereby preventing rapid failure of the SC caused by mechanical damage.Additionally,the photothermal properties of the polydopamine moieties generate a rapid temperature gradient upon light exposure,allowing early-stage damage detection through infrared thermography.This work presents a biomimetic strategy for developing intelligent anticorrosion coatings that combine superhydrophobicity,self-repair,and realtime damage sensing,advancing the application of MOF-derived materials in protective coatings.展开更多
基金supported by the National Natural Science Foundation of China(32272416,31972147)Project of Tianjin Science and Technology Plan(22ZYJDSS00030).
文摘Herein,a novel label-free electrochemical immunosensor was fabricated via immobilizing specific anti-β-lactoglobulin(β-LG)antibodies(Abs)onto an integrated electrode of gold nanoparticles(AuNPs)/Prussian blue(PB)/cubic Ia3d structured mesoporous carbon(CMK-8).This immunosensor allowed for the quantitative detection of the major milk allergenβ-LG.CMK-8 with excellent electrical conductivity and uniformly adjustable pore structure was modified on the glassy carbon electrode(GCE)and served as the sensitive substrate for the electro-polymerization of PB,forming the redox-active layer.AuNPs were subsequently electrochemically deposited on PB/CMK-8/GCE to improve the electrical conductivity and utilized as the connector for Abs immobilization.Duringβ-LG detection,the Abs-modified AuNPs/PB/CMK-8/GCE exhibited a significant reduction in differential pulse voltammetry current signal when exposed toβ-LG,displaying an inverse dose-dependent relationship.The developed electrochemical immunosensor demonstrated good detection performance forβ-LG,with a wider linear range of 0.01-100 ng/mL and a lower detection limit of 4.72 pg/mL.Meanwhile,the sensor exhibited remarkable repeatability,reproducibility,stability and anti-interference capabilities,which was further applied to detectβ-LG in dairy food,achieving satisfactory recoveries(89.2%-98.8%)and lower relative standard deviation(£3.1%).Therefore,this innovative electrochemical method for food allergen detection holds great potential application in food safety determination and evaluation.
基金supported by the National Natural Science Foundation of China(31972147)Project of Tianjin Science and Technology Plan(19PTSYJC00050)+1 种基金the Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology(SKLFNS-KF-202011)Project program of Key Laboratory of Food Nutrition and Safety,Ministry of Education,Tianjin Key Laboratory of Food Nutrition and Safety,China(JYB202002)。
文摘In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk products.Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment,amount of anti-β-LG antibody and regeneration reagent were optimized in detail.Under optimal conditions,the proposed QCM immunochip exhibited good recognition of β-LG,with a calibration curve of ΔF=12.877 C_(β-LG)^(0.4809)(R^(2)=0.9982)and limit of detection of 0.04μg/mL.Additionally,this portable QCM immunochip had good stability,high specificity,and no obvious cross-reaction to three other milk proteins(α-casein,α-lactalbumin,and lactoferrin).It could compete a qualitative measurement within5 min,and could be reused at least ten times.In the β-LG analysis of actual milk samples,the developed QCM immunochip yielded reliable and accurate results,which correlated strongly with those from the standard HPLC method(R^(2)=0.9969).Thus,the portable,stable,and reproducible QCM immunochip developed in this study allowed the rapid,cost-effectively and sensitively measure theβ-LG in milk products.
基金the support from the Natural Sciences and Engineering Research Council of Canada(NSERC)the Canada Research Chairs Program+3 种基金the support from the China Scholarship Councilfinancially supported by the National Natural Science Foundation of China(51871097)the Engineering Research Centre for Material Corrosion and Protection in Colleges and Universities of Guizhou Province,China(Qianjiaoji[2022]057)the Changsha Municipal Natural Science Foundation(kq2502209)。
文摘Superhydrophobic coatings that physically separate metal substrates from aqueous media have emerged as a promising strategy against metal corrosion;however,their practical application is hindered by poor mechanical durability and rapid performance degradation in the harsh environment.Herein,inspired by the granular architecture and dynamic metal coordination chemistry in mussel byssus cuticle,a hierarchical metal coordination-mediated self-adaptive coating(SC)integrating surface superhydrophobicity,self-healing anticorrosion,and damage-monitoring capacity is constructed on steel substrates using the metal-organic framework(MOF)as the multifunctional nanoplatform.Specifically,a MOF-polydopamine nanocomposite coating is fabricated on mild steel via a coordination-dissociation-polymerization mechanism,where the MOF serves as a self-sacrificial template to initiate the deposition of polydopamine,and the SC is obtained after the subsequent hydrophobization via Michael addition and Schiff base reaction.The super-hydrophobic surface of SC with a water contact angle of 160°provides a superior passive barrier against corrosive media,showing a protective efficiency of 97.5%.Furthermore,the MOF-polydopamine interlayer endows the SC with superior corrosion-triggered self-healing properties by forming protective adsorption films at the exposed steel surface,thereby preventing rapid failure of the SC caused by mechanical damage.Additionally,the photothermal properties of the polydopamine moieties generate a rapid temperature gradient upon light exposure,allowing early-stage damage detection through infrared thermography.This work presents a biomimetic strategy for developing intelligent anticorrosion coatings that combine superhydrophobicity,self-repair,and realtime damage sensing,advancing the application of MOF-derived materials in protective coatings.
