Bioactive molecules have shown great promise for effectively regulating various bone formation processes,rendering them attractive therapeutics for bone regeneration.However,the widespread application of bioactive mol...Bioactive molecules have shown great promise for effectively regulating various bone formation processes,rendering them attractive therapeutics for bone regeneration.However,the widespread application of bioactive molecules is limited by their low accumulation and short half-lives in vivo.Hydrogels have emerged as ideal carriers to address these challenges,offering the potential to prolong retention times at lesion sites,extend half-lives in vivo and mitigate side effects,avoid burst release,and promote adsorption under physiological conditions.This review systematically summarizes the recent advances in the development of bioactive molecule-loaded hydrogels for bone regeneration,encompassing applications in cranial defect repair,femoral defect repair,periodontal bone regeneration,and bone regeneration with underlying diseases.Additionally,this review discusses the current strategies aimed at improving the release profiles of bioactive molecules through stimuli-responsive delivery,carrier-assisted delivery,and sequential delivery.Finally,this review elucidates the existing challenges and future directions of hydrogel encapsulated bioactive molecules in the field of bone regeneration.展开更多
Wound repair is a complex physiological process that often leads to bacterial infections,which significantly threaten human health.Therefore,developing wound-healing materials that promote healing and prevent bacteria...Wound repair is a complex physiological process that often leads to bacterial infections,which significantly threaten human health.Therefore,developing wound-healing materials that promote healing and prevent bacterial infections is crucial.In this study,the coordination interaction between sulfhydryl groups on dithiothreitol(DTT)and MoS_(2)nanosheets is investigated to synthesize a MoS_(2)-DTT nanozyme with photothermal properties and an improved free-radical scavenging ability.Double-bond-modified hyaluronic acid is used as a monomer and is cross-linked with a PF127-DA agent.PHMoD is prepared in coordination with MoS_(2)-DTT as the functional component.This hydrogel exhibits antioxidant and antibacterial properties,attributed to the catalytic activity of catalase-like enzymes and photothermal effects.Under the near-infrared(NIR),it exhibits potent antibacterial effects against gram-positive(Staphylococcus aureus)and gram-negative bacteria(Escherichia coli),achieving bactericidal rates of 99.76%and 99.42%,respectively.Furthermore,the hydrogel exhibits remarkable reactive oxygen species scavenging and antioxidant capabilities,effectively countering oxidative stress in L929 cells.Remarkably,in an animal model,wounds treated with the PHMoD(2.0)and NIR laser heal the fastest,sealing completely within 10 days.These results indicate the unique biocompatibility and bifunctionality of the PHMoD,which make it a promising material for wound-healing applications.展开更多
基金supported by the National Natural Science Foundation of China(51925304)Natural Science Foundation of Sichuan Province(2024NSFSC1023)Medical Research Program of Sichuan Province(Q23015).
文摘Bioactive molecules have shown great promise for effectively regulating various bone formation processes,rendering them attractive therapeutics for bone regeneration.However,the widespread application of bioactive molecules is limited by their low accumulation and short half-lives in vivo.Hydrogels have emerged as ideal carriers to address these challenges,offering the potential to prolong retention times at lesion sites,extend half-lives in vivo and mitigate side effects,avoid burst release,and promote adsorption under physiological conditions.This review systematically summarizes the recent advances in the development of bioactive molecule-loaded hydrogels for bone regeneration,encompassing applications in cranial defect repair,femoral defect repair,periodontal bone regeneration,and bone regeneration with underlying diseases.Additionally,this review discusses the current strategies aimed at improving the release profiles of bioactive molecules through stimuli-responsive delivery,carrier-assisted delivery,and sequential delivery.Finally,this review elucidates the existing challenges and future directions of hydrogel encapsulated bioactive molecules in the field of bone regeneration.
基金National Natural Science Foundation of China(32371384 and 52173140)Natural Science Foundation of Sichuan(2023NSFSC0329)+5 种基金Popularization and Application Project of Health Commission of Sichuan Province(20PJ279)Province and Science and Technology Plan Project Innovation and Entrepreneurship Seedling Project of Guangyuan(22MZGC0002)Fundamental Research Funds for the Central Universities(A0920502052301-477)Guiding Science and Technology Programme Projects of Guangyuan(23ZDYF0052)Sichuan Provincial Science and Technology Planned Projects(24NSFSC7829)Sichuan Provincial Medical Youth Innovative Scientific Research Project Program(Q23015).
文摘Wound repair is a complex physiological process that often leads to bacterial infections,which significantly threaten human health.Therefore,developing wound-healing materials that promote healing and prevent bacterial infections is crucial.In this study,the coordination interaction between sulfhydryl groups on dithiothreitol(DTT)and MoS_(2)nanosheets is investigated to synthesize a MoS_(2)-DTT nanozyme with photothermal properties and an improved free-radical scavenging ability.Double-bond-modified hyaluronic acid is used as a monomer and is cross-linked with a PF127-DA agent.PHMoD is prepared in coordination with MoS_(2)-DTT as the functional component.This hydrogel exhibits antioxidant and antibacterial properties,attributed to the catalytic activity of catalase-like enzymes and photothermal effects.Under the near-infrared(NIR),it exhibits potent antibacterial effects against gram-positive(Staphylococcus aureus)and gram-negative bacteria(Escherichia coli),achieving bactericidal rates of 99.76%and 99.42%,respectively.Furthermore,the hydrogel exhibits remarkable reactive oxygen species scavenging and antioxidant capabilities,effectively countering oxidative stress in L929 cells.Remarkably,in an animal model,wounds treated with the PHMoD(2.0)and NIR laser heal the fastest,sealing completely within 10 days.These results indicate the unique biocompatibility and bifunctionality of the PHMoD,which make it a promising material for wound-healing applications.
