The influence of essential (Cu, Ni and Zn) and non-essential heavy metals (Hg, Cr, Pb and Cd) on seed germination and early seedling growth in industrial oil crop Crambe abyssinica was evaluated under laboratory condi...The influence of essential (Cu, Ni and Zn) and non-essential heavy metals (Hg, Cr, Pb and Cd) on seed germination and early seedling growth in industrial oil crop Crambe abyssinica was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested only Cu and Hg significantly (P < 0.01) decreased Crambe seed germination in a dose-dependent manner at higher concentrations while certain Cr concentrations significantly increased the seed germination (P < 0.05). All the 7 heavy metals decreased significantly relative root length, shoot length and fresh seedling weight in a dose-dependent manner (P < 0.01). The heavy metals except Ni decreased relative root length first, then shoot length or fresh seedling weight, and finally seed germination. Ni seemed to influence the relative fresh seedling weight first, then shoot length, root length and finally seed germination at lower concentrations, but the decrease in relative root length became faster when the Ni concentrations were increased. Our results indicated that Crambe is tolerant or moderately tolerant to the heavy metals tested except Ni and can be improved for phytoremediation of soils contaminated by heavy metals.展开更多
Heavy metals present in soil and water naturally or as contaminants from human activities can cause bioaccumulation affecting the entire ecosystem and pose harmful health consequences in all life forms. Some famous no...Heavy metals present in soil and water naturally or as contaminants from human activities can cause bioaccumulation affecting the entire ecosystem and pose harmful health consequences in all life forms. Some famous non-food hyperaccumulators such as Thlaspi caerulescens, Sedum alfredii, Pteris vittata, Arabidopsis halleri and Athyrium yokoscense are of very little economic value, making it difficult for them to be used for phytoremediation. In this paper, the influence of heavy metals Cu, Ni, Zn, Hg, Cr, Pb and Cd on seed germination and early seedling growth in oil crop Eruca sativa was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested, only Ni at higher concentrations (1 mM and above) significantly decreased the Eruca seed germination in a dose-dependent manner. All heavy metals except Zn and Ni decreased the root length first, then the shoot length or the fresh seedling weight, and seed germination was always the last to be influenced. With Ni, the root length, shoot length and fresh seedling weight were stimulated when Ni concentrations were under 1 mM;with Zn, the root length, shoot length and fresh seedling weight were increased by all concentrations tested (0.20 - 5.0 mM). Our results indicated that Eruca is tolerant or moderately tolerant to Cu, Hg, Cr, and Cd and highly tolerant to Pb, Ni and Zn, and can be developed as an industrial oil crop for phytoremediation of soils contaminated by heavy metals.展开更多
Cells need to coordinate gene expression with their metabolic states to maintain cell homeostasis and growth.However,how cells transduce nutrient availability to appropriate gene expression response via histone modifi...Cells need to coordinate gene expression with their metabolic states to maintain cell homeostasis and growth.However,how cells transduce nutrient availability to appropriate gene expression response via histone modifications remains largely unknown.Here,we report that glucose specifically induces histone H3K4 trimethylation(H3K4me3),an evolutionarily conserved histone covalent modification associated with active gene transcription,and that glycolytic enzymes and metabolites are required for this in?duction.Although glycolysis supplies S-adenosylmethionine for histone methyltransferase Setl to catalyze H3K4me3,glucose induces H3K4me3 primarily by inhibiting histone demethylase Jhd2-catalyzed H3K4 demethylation.Glycolysis provides acetyl-CoA to stimulate histone acetyltransferase Gcn5 to acetylate H3K14,which then inhibits the binding of Jhd2 to chromatin to increase H3K4me3.By repressing Jhd2-mediated H3K4 demethylation,glycolytic enzymes regulate gene expression and cell survival during chronological aging.Thus,our results elucidate how cells reprogram their gene expression programs in response to glucose availability via histone modifications.展开更多
文摘The influence of essential (Cu, Ni and Zn) and non-essential heavy metals (Hg, Cr, Pb and Cd) on seed germination and early seedling growth in industrial oil crop Crambe abyssinica was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested only Cu and Hg significantly (P < 0.01) decreased Crambe seed germination in a dose-dependent manner at higher concentrations while certain Cr concentrations significantly increased the seed germination (P < 0.05). All the 7 heavy metals decreased significantly relative root length, shoot length and fresh seedling weight in a dose-dependent manner (P < 0.01). The heavy metals except Ni decreased relative root length first, then shoot length or fresh seedling weight, and finally seed germination. Ni seemed to influence the relative fresh seedling weight first, then shoot length, root length and finally seed germination at lower concentrations, but the decrease in relative root length became faster when the Ni concentrations were increased. Our results indicated that Crambe is tolerant or moderately tolerant to the heavy metals tested except Ni and can be improved for phytoremediation of soils contaminated by heavy metals.
文摘Heavy metals present in soil and water naturally or as contaminants from human activities can cause bioaccumulation affecting the entire ecosystem and pose harmful health consequences in all life forms. Some famous non-food hyperaccumulators such as Thlaspi caerulescens, Sedum alfredii, Pteris vittata, Arabidopsis halleri and Athyrium yokoscense are of very little economic value, making it difficult for them to be used for phytoremediation. In this paper, the influence of heavy metals Cu, Ni, Zn, Hg, Cr, Pb and Cd on seed germination and early seedling growth in oil crop Eruca sativa was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested, only Ni at higher concentrations (1 mM and above) significantly decreased the Eruca seed germination in a dose-dependent manner. All heavy metals except Zn and Ni decreased the root length first, then the shoot length or the fresh seedling weight, and seed germination was always the last to be influenced. With Ni, the root length, shoot length and fresh seedling weight were stimulated when Ni concentrations were under 1 mM;with Zn, the root length, shoot length and fresh seedling weight were increased by all concentrations tested (0.20 - 5.0 mM). Our results indicated that Eruca is tolerant or moderately tolerant to Cu, Hg, Cr, and Cd and highly tolerant to Pb, Ni and Zn, and can be developed as an industrial oil crop for phytoremediation of soils contaminated by heavy metals.
基金supported by grants from the National Natural Science Foundation of China(31970578,31872812,31671335,and 31600046)the Natural Science Foundation of Hubei Province(2017CFA066).
文摘Cells need to coordinate gene expression with their metabolic states to maintain cell homeostasis and growth.However,how cells transduce nutrient availability to appropriate gene expression response via histone modifications remains largely unknown.Here,we report that glucose specifically induces histone H3K4 trimethylation(H3K4me3),an evolutionarily conserved histone covalent modification associated with active gene transcription,and that glycolytic enzymes and metabolites are required for this in?duction.Although glycolysis supplies S-adenosylmethionine for histone methyltransferase Setl to catalyze H3K4me3,glucose induces H3K4me3 primarily by inhibiting histone demethylase Jhd2-catalyzed H3K4 demethylation.Glycolysis provides acetyl-CoA to stimulate histone acetyltransferase Gcn5 to acetylate H3K14,which then inhibits the binding of Jhd2 to chromatin to increase H3K4me3.By repressing Jhd2-mediated H3K4 demethylation,glycolytic enzymes regulate gene expression and cell survival during chronological aging.Thus,our results elucidate how cells reprogram their gene expression programs in response to glucose availability via histone modifications.
