Normal-phase and reversed-phase silica gel column chromatography and semi-preparative high performance liquid chromatography were applied to study the chemical constituents of the stems and twigs of the Hainan mangrov...Normal-phase and reversed-phase silica gel column chromatography and semi-preparative high performance liquid chromatography were applied to study the chemical constituents of the stems and twigs of the Hainan mangrove plant,Excoecaria agallocha L.A new 3,4-seco-ent-kaurane diterpenoid,named agallochanin acid A(1),and a rare ent-tetranorlabdane diterpenoid,named agallochanin acid B(2),along with four known diterpenoids were isolated.Their structures including absolute configurations were characterized via HR-ESI-MS,extensive 1D and 2D NMR investigations and calculated and experimental electronic circular dichroism(ECD)data.All isolates were tested for their inhibitory effects on NO production in lipopolysaccharide(LPS)-activated BV-2 cells.ent-3,4-Seco-17-oxo-kaur-4(19),15(16)-dien-3-oic acid(3)and ent-15-hydroxy-labda-8(17),13E-dien-3-one(5)exhibited considerable anti-neuroinflammatory activities with IC50 values of(14.32±1.99)and(4.24±0.37)μmol/L.展开更多
文摘Normal-phase and reversed-phase silica gel column chromatography and semi-preparative high performance liquid chromatography were applied to study the chemical constituents of the stems and twigs of the Hainan mangrove plant,Excoecaria agallocha L.A new 3,4-seco-ent-kaurane diterpenoid,named agallochanin acid A(1),and a rare ent-tetranorlabdane diterpenoid,named agallochanin acid B(2),along with four known diterpenoids were isolated.Their structures including absolute configurations were characterized via HR-ESI-MS,extensive 1D and 2D NMR investigations and calculated and experimental electronic circular dichroism(ECD)data.All isolates were tested for their inhibitory effects on NO production in lipopolysaccharide(LPS)-activated BV-2 cells.ent-3,4-Seco-17-oxo-kaur-4(19),15(16)-dien-3-oic acid(3)and ent-15-hydroxy-labda-8(17),13E-dien-3-one(5)exhibited considerable anti-neuroinflammatory activities with IC50 values of(14.32±1.99)and(4.24±0.37)μmol/L.
