Malus x domestica microRNA MdmiR285N is a potential key regulator of plant immunity,as it has been predicted to target 35 RNA transcripts coding for different disease resistance proteins involved in plant defense to p...Malus x domestica microRNA MdmiR285N is a potential key regulator of plant immunity,as it has been predicted to target 35 RNA transcripts coding for different disease resistance proteins involved in plant defense to pathogens.In this study,the promoter region of MdmiR285N was isolated from the apple genome and analyzed in silico to detect potential regulatory regions controlling its transcription.A complex network of putative regulatory elements involved in plant growth and development,and in response to different hormones and stress conditions,was identified.Activity of theβ-Glucoronidase(GUS)reporter gene driven by the promoter of MdmiR285N was examined in transgenic apple,demonstrating that MdmiR285N was expressed during the vegetative growth phase.Similarly,in transgenic Arabidopsis thaliana,spatial and temporal patterns of GUS expression revealed that MdmiR285N was differentially regulated during seed germination,vegetative phase change,and reproductive development.To elucidate the role of MdmiR285N in plant immunity,MdmiR285N expression in wild-type apple plants and GUS activity in transgenic apple and Arabidopsis thaliana plants were monitored in response to Erwinia amylovora and Pseudomonas syringae pv.Tomato DC3000.A significant decrease of MdmiR285N levels and GUS expression was observed during host-pathogen infections.Overall,these data suggest that MdmiR285N is involved in the biotic stress response,plant growth,and reproductive development.展开更多
Physcomitrium eurystomum Sendtn.is a very rare European ephemeral funaroid moss.The entire European population of this species is considered threatened and it is red-listed in many regions and countries.In addition to...Physcomitrium eurystomum Sendtn.is a very rare European ephemeral funaroid moss.The entire European population of this species is considered threatened and it is red-listed in many regions and countries.In addition to being recognized as threatened and included in nature conservation legislation,it also requires active protec-tion measures.This study aims to contribute to effective conservation practices for P.eurystomum.Different con-servation physiology tests were carried out to propagate this species to achieve a reliable procedure for biomass production and the potential reintroduction of germplasm.Ex situ tests,both in vitro and ex vitro,were carried out to determine the optimal method for spore production under laboratory conditions,considering that the spores are the best propagules for the reintroduction of the species.An important outcome of this research is a deeper understanding of the role of the spore bank of this species for its survival in a variable environment.Some additional characterizations of the biology of this ephemeral moss species are also discussed,such as the functioning of the photosynthetic apparatus of the vegetative vs.sexual phases to clarify the transition to the reproductive phase.It is proposed that both types of sporophytes be used in conservation practices due to viable spore bank self-sustainability in rapidly changing environmental conditions.展开更多
文摘Malus x domestica microRNA MdmiR285N is a potential key regulator of plant immunity,as it has been predicted to target 35 RNA transcripts coding for different disease resistance proteins involved in plant defense to pathogens.In this study,the promoter region of MdmiR285N was isolated from the apple genome and analyzed in silico to detect potential regulatory regions controlling its transcription.A complex network of putative regulatory elements involved in plant growth and development,and in response to different hormones and stress conditions,was identified.Activity of theβ-Glucoronidase(GUS)reporter gene driven by the promoter of MdmiR285N was examined in transgenic apple,demonstrating that MdmiR285N was expressed during the vegetative growth phase.Similarly,in transgenic Arabidopsis thaliana,spatial and temporal patterns of GUS expression revealed that MdmiR285N was differentially regulated during seed germination,vegetative phase change,and reproductive development.To elucidate the role of MdmiR285N in plant immunity,MdmiR285N expression in wild-type apple plants and GUS activity in transgenic apple and Arabidopsis thaliana plants were monitored in response to Erwinia amylovora and Pseudomonas syringae pv.Tomato DC3000.A significant decrease of MdmiR285N levels and GUS expression was observed during host-pathogen infections.Overall,these data suggest that MdmiR285N is involved in the biotic stress response,plant growth,and reproductive development.
基金The project is supported by the Serbian Ministry of Science,Technological Development and Innovations,Contract Nos.451-03-65/2024-03/200178 and 451-03-66/2024-03/200178.
文摘Physcomitrium eurystomum Sendtn.is a very rare European ephemeral funaroid moss.The entire European population of this species is considered threatened and it is red-listed in many regions and countries.In addition to being recognized as threatened and included in nature conservation legislation,it also requires active protec-tion measures.This study aims to contribute to effective conservation practices for P.eurystomum.Different con-servation physiology tests were carried out to propagate this species to achieve a reliable procedure for biomass production and the potential reintroduction of germplasm.Ex situ tests,both in vitro and ex vitro,were carried out to determine the optimal method for spore production under laboratory conditions,considering that the spores are the best propagules for the reintroduction of the species.An important outcome of this research is a deeper understanding of the role of the spore bank of this species for its survival in a variable environment.Some additional characterizations of the biology of this ephemeral moss species are also discussed,such as the functioning of the photosynthetic apparatus of the vegetative vs.sexual phases to clarify the transition to the reproductive phase.It is proposed that both types of sporophytes be used in conservation practices due to viable spore bank self-sustainability in rapidly changing environmental conditions.
