In this study,a denitrification(DN)–partial nitritation(PN)–anaerobic ammonia oxidation(Anammox)system for the efficient nitrogen removal of mature landfill leachate was built with a zone-partitioning self-reflux bi...In this study,a denitrification(DN)–partial nitritation(PN)–anaerobic ammonia oxidation(Anammox)system for the efficient nitrogen removal of mature landfill leachate was built with a zone-partitioning self-reflux biological reactor as the core device,and the effects of changes in seasonal temperature on the nitrogen removal in non-temperature-control environment were explored.The results showed that as the seasonal temperature decreased from 34℃to 11.3℃,the total nitrogen removal rate of the DN-PN-Anammox system gradually decreased from the peak value of 1.42 kg/(m^(3)·day)to 0.49 kg/(m^(3)·day).At low temperatures(<20℃),when the nitrogen load(NLR)of the system is not appropriate,the fluctuation of high NH_(4)^(+)-N concentration in the landfill leachate greatly influenced the stability of the nitrogen removal.At temperatures of 11℃–15℃,the NLR of the system is controlled below 0.5 kg/(m^(3)·day),which can achieve stable nitrogen removal and the nitrogen removal efficiency can reach above 96%.The abundance of Candidatus Brocadia gradually increased with the decrease of temperature.Nitrosomonas,Candidatus Brocadia and Candidatus Kuenenia as the main functional microorganisms in the low temperature.展开更多
Objective To identify clinical characteristics associated with the minimum lumen area （MLA） of proximal or middle intermediate lesions in the left anterior descending （LAD） artery, and to develop a model to predic...Objective To identify clinical characteristics associated with the minimum lumen area （MLA） of proximal or middle intermediate lesions in the left anterior descending （LAD） artery, and to develop a model to predict MLA. Methods We retrospectively analyzed demographic data, medical history, and intravascular ultrasound findings for 90 patients with intermediate lesions in the LAD artery. Linear regression was used to identify factors affecting MLA, and multiple regression was used to develop a model for predicting MLA. Results Age, number of lesions, and diabetes mellitus correlated significantly with MLA of proximal or middle intermediate lesions. A regression model for predicting MLA （mm2） was derived from the data： 7.00 - 0.05 × （age） - 0.50 × （number of lesions）. A cut-off value of 3.1 mm2 was proposed for deciding when to perform percutaneous coronary intervention. Conclusion This model for predicting MLA of proximal or middle intermediate lesions in the LAD artery showed high accuracy, sensitivity, and specificity, indicating good diagnostic potential.展开更多
Numerous models have been proposed to reduce the classification error of Naive Bayes by weakening its attribute independence assumption and some have demonstrated remarkable error performance. Considering that ensembl...Numerous models have been proposed to reduce the classification error of Naive Bayes by weakening its attribute independence assumption and some have demonstrated remarkable error performance. Considering that ensemble learning is an effective method of reducing the classifmation error of the classifier, this paper proposes a double-layer Bayesian classifier ensembles （DLBCE） algorithm based on frequent itemsets. DLBCE constructs a double-layer Bayesian classifier （DLBC） for each frequent itemset the new instance contained and finally ensembles all the classifiers by assigning different weight to different classifier according to the conditional mutual information. The experimental results show that the proposed algorithm outperforms other outstanding algorithms.展开更多
Objective： To compare the plaque composition between stable and unstable plaques, characterize unstable plaque by using iMap-intravascular ultrasound （IVUS）, and quantify the diagnostic criteria for unstable plaque...Objective： To compare the plaque composition between stable and unstable plaques, characterize unstable plaque by using iMap-intravascular ultrasound （IVUS）, and quantify the diagnostic criteria for unstable plaque. Methods： Thirty-three acute coronary syndrome （ACS） patients who had undergone coronary angiography and IVUS from February 19, 2014 to December 19, 2014 at Peking University People＇s Hospital were enrolled in the study. Baseline data were collected. The patients were divided into two groups according to their gray-scale IVUS imaging, stable plaque and unstable plaque. A difference-in-difference evaluation was performed using the baseline data and off-line iMap imaging results between the two groups. A receiver operating characteristic （ROC） curve was constructed to obtain the optimal cut-off value to diagnose unstable plaque. Results： Percentages of fibrotic and necrotic tissues, absolute values of lipidic, necrotic, and calcified tissues, and plaque burden were independent predictors for unstable plaque. Absolute necrotic area was the best predictor and exhibited the highest diagnostic value for plaque vulnerability （area under the curve （AUC）=0.806, P=0.000, 95% CI （0.718, 0.894））. The cut-off score for predicting unstable plaque was 4.0 mm2. Conclusions： This study attempted to propose a cut-off value based on absolute necrotic area using iMap-IVUS to predict plaque vulnerability in patients with ACS. This score might provide a valuable reference for diagnosing unstable plaque.展开更多
The typical model, which involves the measures： support, confidence, and interest, is often adapted to mining association rules. In the model, the related parameters are usually chosen by experience; consequently, th...The typical model, which involves the measures： support, confidence, and interest, is often adapted to mining association rules. In the model, the related parameters are usually chosen by experience; consequently, the number of useful rules is hard to estimate. If the number is too large, we cannot effectively extract the meaningful rules. This paper analyzes the meanings of the parameters and designs a variety of equations between the number of rules and the parameters by using regression method. Finally, we experimentally obtain a preferable regression equation. This paper uses multiple correlation coeficients to test the fitting efiects of the equations and uses significance test to verify whether the coeficients of parameters are significantly zero or not. The regression equation that has a larger multiple correlation coeficient will be chosen as the optimally fitted equation. With the selected optimal equation, we can predict the number of rules under the given parameters and further optimize the choice of the three parameters and determine their ranges of values.展开更多
An eigenvalue method considering the membrane vibration of wrinkling out-of-plane deformation is introduced, and the stress distributing rule in membrane wrinkled area is analyzed. A dynamic analytical model of rectan...An eigenvalue method considering the membrane vibration of wrinkling out-of-plane deformation is introduced, and the stress distributing rule in membrane wrinkled area is analyzed. A dynamic analytical model of rectangular shear wrinkled membrane and its numerical analysis approach are also developed. Results indicate that the stress in wrinkled area is not uniform, i.e. it is larger in wrinkling wave peaks along wrinkles and two ends of wrinkle in vertical direction. Vibration modes of wrinkled membrane are strongly correlated with the wrinkling configurations. The rigidity is larger due to the heavier stress in the part of wrinkling wave peaks. Therefore, wave peaks are always located at the node lines of vibration mode. The vibration frequency obviously increases with the vibration of wave peaks.展开更多
Objective:This study compared the long-term outcomes between rotational atherectomy(RA)for specific indications and on-label use of RA for severely calcified coronary lesions.Methods:Data for patients who underwent RA...Objective:This study compared the long-term outcomes between rotational atherectomy(RA)for specific indications and on-label use of RA for severely calcified coronary lesions.Methods:Data for patients who underwent RA between 2015 and 2020 in a single-center registry were analyzed.The specific indication group included patients with ostial lesions,unprotected left main coronary artery stenosis,chronic total occlusions,stent ablation,angulated lesions,and cardiac dysfunction,whereas patients with none of the above-mentioned characteristics were included in the on-label group.The primary endpoint was compared between groups.Results:A total of 176 patients in the on-label group and 125 patients in the specific indication group were included.Patient clinical characteristics were comparable between groups.The incidence of complications during the procedure was higher in the specific indication group than in the on-label group(20.0%vs.10.8%,P=0.018).No significant dif-ference was observed in in-hospital MACCE between groups(12.5%vs 9.7%,P=0.392).During 35(10–57)months of follow-up,MACCE occurred in 46 patients(15.3%).The incidence of MACCE was much higher in the specific indication group than the on-label group(25.6%vs 13.6%,P=0.034).Conclusions:RA for specific indications,compared with on-label use,had a higher incidence of complications dur-ing the procedure and poorer long-term clinical outcomes.展开更多
Optically induced electroporation(OIE)is a promising microfluidic-based approach for the electroporation of cell membranes.However,previously proposed microfluidic cell-electroporation devices required tedious sample ...Optically induced electroporation(OIE)is a promising microfluidic-based approach for the electroporation of cell membranes.However,previously proposed microfluidic cell-electroporation devices required tedious sample pre-treatment steps,specifically,periodic media exchange.To enable the use of this OIE process in a practical protocol,we developed a new design for a microfluidic device that can perform continuous OIE;i.e.,it is capable of automatically replacing the culture medium with electroporation buffers.Integrating medium exchanges on-chip with OIE minimises critical issues such as cell loss and damage,both of which are common in traditional,centrifuge-based approaches.Most importantly,our new system is suitable for handling small or rare cell populations.Two medium exchange modules,including a micropost array railing structure and a deterministic lateral displacement structure,were first adopted and optimised for medium exchange and then integrated with the OIE module.The efficacy of these integrated microfluidic systems was demonstrated by transfecting an enhanced green fluorescent protein(EGFP)plasmid into human embryonic kidney 293T cells,with an efficiency of 8.3%.This result is the highest efficiency reported for any existing OIE-based microfluidic system.In addition,successful co-transfections of three distinct plasmids(EGFP,DsRed and ECFP)into cells were successfully achieved.Hence,we demonstrated that this system is capable of automatically performing multiple gene transfections into mammalian cells.展开更多
基金supported by the Natural Science Foundation of China(No.51938010)the Suzhou Science and Technology Plan Project-Minsheng Project(No.SS201804-02)+1 种基金National Key Research and Development Programme of China(No.2016YFC 0401103)the Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment。
文摘In this study,a denitrification(DN)–partial nitritation(PN)–anaerobic ammonia oxidation(Anammox)system for the efficient nitrogen removal of mature landfill leachate was built with a zone-partitioning self-reflux biological reactor as the core device,and the effects of changes in seasonal temperature on the nitrogen removal in non-temperature-control environment were explored.The results showed that as the seasonal temperature decreased from 34℃to 11.3℃,the total nitrogen removal rate of the DN-PN-Anammox system gradually decreased from the peak value of 1.42 kg/(m^(3)·day)to 0.49 kg/(m^(3)·day).At low temperatures(<20℃),when the nitrogen load(NLR)of the system is not appropriate,the fluctuation of high NH_(4)^(+)-N concentration in the landfill leachate greatly influenced the stability of the nitrogen removal.At temperatures of 11℃–15℃,the NLR of the system is controlled below 0.5 kg/(m^(3)·day),which can achieve stable nitrogen removal and the nitrogen removal efficiency can reach above 96%.The abundance of Candidatus Brocadia gradually increased with the decrease of temperature.Nitrosomonas,Candidatus Brocadia and Candidatus Kuenenia as the main functional microorganisms in the low temperature.
文摘Objective To identify clinical characteristics associated with the minimum lumen area （MLA） of proximal or middle intermediate lesions in the left anterior descending （LAD） artery, and to develop a model to predict MLA. Methods We retrospectively analyzed demographic data, medical history, and intravascular ultrasound findings for 90 patients with intermediate lesions in the LAD artery. Linear regression was used to identify factors affecting MLA, and multiple regression was used to develop a model for predicting MLA. Results Age, number of lesions, and diabetes mellitus correlated significantly with MLA of proximal or middle intermediate lesions. A regression model for predicting MLA （mm2） was derived from the data： 7.00 - 0.05 × （age） - 0.50 × （number of lesions）. A cut-off value of 3.1 mm2 was proposed for deciding when to perform percutaneous coronary intervention. Conclusion This model for predicting MLA of proximal or middle intermediate lesions in the LAD artery showed high accuracy, sensitivity, and specificity, indicating good diagnostic potential.
基金supported by National Natural Science Foundation of China (Nos. 61073133, 60973067, and 61175053)Fundamental Research Funds for the Central Universities of China(No. 2011ZD010)
文摘Numerous models have been proposed to reduce the classification error of Naive Bayes by weakening its attribute independence assumption and some have demonstrated remarkable error performance. Considering that ensemble learning is an effective method of reducing the classifmation error of the classifier, this paper proposes a double-layer Bayesian classifier ensembles （DLBCE） algorithm based on frequent itemsets. DLBCE constructs a double-layer Bayesian classifier （DLBC） for each frequent itemset the new instance contained and finally ensembles all the classifiers by assigning different weight to different classifier according to the conditional mutual information. The experimental results show that the proposed algorithm outperforms other outstanding algorithms.
基金supported by the Capital Health Research and Development of Special(No.2014-2-4085),China
文摘Objective： To compare the plaque composition between stable and unstable plaques, characterize unstable plaque by using iMap-intravascular ultrasound （IVUS）, and quantify the diagnostic criteria for unstable plaque. Methods： Thirty-three acute coronary syndrome （ACS） patients who had undergone coronary angiography and IVUS from February 19, 2014 to December 19, 2014 at Peking University People＇s Hospital were enrolled in the study. Baseline data were collected. The patients were divided into two groups according to their gray-scale IVUS imaging, stable plaque and unstable plaque. A difference-in-difference evaluation was performed using the baseline data and off-line iMap imaging results between the two groups. A receiver operating characteristic （ROC） curve was constructed to obtain the optimal cut-off value to diagnose unstable plaque. Results： Percentages of fibrotic and necrotic tissues, absolute values of lipidic, necrotic, and calcified tissues, and plaque burden were independent predictors for unstable plaque. Absolute necrotic area was the best predictor and exhibited the highest diagnostic value for plaque vulnerability （area under the curve （AUC）=0.806, P=0.000, 95% CI （0.718, 0.894））. The cut-off score for predicting unstable plaque was 4.0 mm2. Conclusions： This study attempted to propose a cut-off value based on absolute necrotic area using iMap-IVUS to predict plaque vulnerability in patients with ACS. This score might provide a valuable reference for diagnosing unstable plaque.
基金supported by the National Natural Science Foundation of China (No. J07240003, No. 60773084, No. 60603023)National Research Fund for the Doctoral Program of Higher Education of China (No. 20070151009)
文摘The typical model, which involves the measures： support, confidence, and interest, is often adapted to mining association rules. In the model, the related parameters are usually chosen by experience; consequently, the number of useful rules is hard to estimate. If the number is too large, we cannot effectively extract the meaningful rules. This paper analyzes the meanings of the parameters and designs a variety of equations between the number of rules and the parameters by using regression method. Finally, we experimentally obtain a preferable regression equation. This paper uses multiple correlation coeficients to test the fitting efiects of the equations and uses significance test to verify whether the coeficients of parameters are significantly zero or not. The regression equation that has a larger multiple correlation coeficient will be chosen as the optimally fitted equation. With the selected optimal equation, we can predict the number of rules under the given parameters and further optimize the choice of the three parameters and determine their ranges of values.
文摘An eigenvalue method considering the membrane vibration of wrinkling out-of-plane deformation is introduced, and the stress distributing rule in membrane wrinkled area is analyzed. A dynamic analytical model of rectangular shear wrinkled membrane and its numerical analysis approach are also developed. Results indicate that the stress in wrinkled area is not uniform, i.e. it is larger in wrinkling wave peaks along wrinkles and two ends of wrinkle in vertical direction. Vibration modes of wrinkled membrane are strongly correlated with the wrinkling configurations. The rigidity is larger due to the heavier stress in the part of wrinkling wave peaks. Therefore, wave peaks are always located at the node lines of vibration mode. The vibration frequency obviously increases with the vibration of wave peaks.
基金This study was supported by the National Natural Science Foundation of China(grant No.81800316).
文摘Objective:This study compared the long-term outcomes between rotational atherectomy(RA)for specific indications and on-label use of RA for severely calcified coronary lesions.Methods:Data for patients who underwent RA between 2015 and 2020 in a single-center registry were analyzed.The specific indication group included patients with ostial lesions,unprotected left main coronary artery stenosis,chronic total occlusions,stent ablation,angulated lesions,and cardiac dysfunction,whereas patients with none of the above-mentioned characteristics were included in the on-label group.The primary endpoint was compared between groups.Results:A total of 176 patients in the on-label group and 125 patients in the specific indication group were included.Patient clinical characteristics were comparable between groups.The incidence of complications during the procedure was higher in the specific indication group than in the on-label group(20.0%vs.10.8%,P=0.018).No significant dif-ference was observed in in-hospital MACCE between groups(12.5%vs 9.7%,P=0.392).During 35(10–57)months of follow-up,MACCE occurred in 46 patients(15.3%).The incidence of MACCE was much higher in the specific indication group than the on-label group(25.6%vs 13.6%,P=0.034).Conclusions:RA for specific indications,compared with on-label use,had a higher incidence of complications dur-ing the procedure and poorer long-term clinical outcomes.
基金The authors gratefully acknowledge the financial support provided to this study by“the National Science Council in Taiwan(NSC102-2218-E-007-001)”.
文摘Optically induced electroporation(OIE)is a promising microfluidic-based approach for the electroporation of cell membranes.However,previously proposed microfluidic cell-electroporation devices required tedious sample pre-treatment steps,specifically,periodic media exchange.To enable the use of this OIE process in a practical protocol,we developed a new design for a microfluidic device that can perform continuous OIE;i.e.,it is capable of automatically replacing the culture medium with electroporation buffers.Integrating medium exchanges on-chip with OIE minimises critical issues such as cell loss and damage,both of which are common in traditional,centrifuge-based approaches.Most importantly,our new system is suitable for handling small or rare cell populations.Two medium exchange modules,including a micropost array railing structure and a deterministic lateral displacement structure,were first adopted and optimised for medium exchange and then integrated with the OIE module.The efficacy of these integrated microfluidic systems was demonstrated by transfecting an enhanced green fluorescent protein(EGFP)plasmid into human embryonic kidney 293T cells,with an efficiency of 8.3%.This result is the highest efficiency reported for any existing OIE-based microfluidic system.In addition,successful co-transfections of three distinct plasmids(EGFP,DsRed and ECFP)into cells were successfully achieved.Hence,we demonstrated that this system is capable of automatically performing multiple gene transfections into mammalian cells.
