Objective: To determine the expression of non-muscle myosin heavy chain 9(MYH9) in osteosarcoma and its effect on the migration and invasion abilities of tumor cell. Methods: A total of 65 cases of osteosarcoma and 20...Objective: To determine the expression of non-muscle myosin heavy chain 9(MYH9) in osteosarcoma and its effect on the migration and invasion abilities of tumor cell. Methods: A total of 65 cases of osteosarcoma and 20 cases with benign osteochondroma who underwent resection operation in the Orthopaedics Department of our hospital from January 1st 2009 to January 1st 2015 were selected. Their mR NA levels of MYH9 were tested by q rt-PCR. Immunohistochemical method was used to examine the expression of MYH9 in osteosarcoma and the correlation between the positive expression of MYH9 and the clinicopathological features of patients was illustrated by statistical analysis. MYH9 was compounded artificially. The expression of MYH9 in SAOS2 osteosarcoma cells was decreased by si RNA. Scratch test was used to determine the change of SAOS2 cell migration ability after MYH9 silence. Transwell assay was employed to detect the change of cell invasion ability after MYH9 silence.Results: The expression levels of m RNA of MYH9 and protein in osteosarcoma tissues were significant higher than those in benign osteochondroma tissues. The high expression of MYH9 in osteosarcoma tissues was apparently related to the high Enneking classification(III classification) and lung metastasis. SiR NA of MYH9 could evidently decrease the expression level of MYH9 in SAOS2. The down-regulated expression of MYH9 could inhibit the migration and invasion abilities of SAOS2 cells. Conclusions: MYH9 shows a trend of high expression in osteosarcoma tissues, and its high expression is associated with features such as tumor invasion and metastasis. The down-regulated MYH9 can realize an anti-tumor effect by inhibiting the migration and invasion of osteosarcoma cells.展开更多
Objective:To get scientific basis for further health education through the research of the road construction workers'KBP before and after the interventions of highway AIDS prevention project.Methods:Multi-stage ra...Objective:To get scientific basis for further health education through the research of the road construction workers'KBP before and after the interventions of highway AIDS prevention project.Methods:Multi-stage random sampling method was employeed to select workers of 8 sites from 14 sites along highway to investigate their AIDS knowledge,belief and performance(KBP)before and after highway AIDS prevention project.Results:Over 90%of the investigated workers had ever heard about AIDS,and the non-skilled workers of lower educational level improved more after intervention.The correct answer rate of the three transmitting ways of AIDS of drivers which is the focused group of highway before and after intervention had the obvious statistical significance(P<0.05),and the other group's correct answer rates also had improved after intervention.Most people's understanding of preventing AIDS through correct use of condoms when having sex had a statistically significanl differenee(P<0.05)after prevention.The rates of using condoms of foremen and skilled workers when having sex with commercial sex worker/casual partner increased after intervention.Conclusions:The health education of HIV among the road construction workers is effective and further health education of HIV prevention should be cairied out among the road construction workers to improve their knowledge and awareness of avoiding the high-risk behaviors.展开更多
High-entropy oxides(HEOs)are a new class of single-phase structures with unique electronic and catalytic properties;therefore,it is worthwhile to explore their applications in electrocatalysis.In this study,a Pt/(FeCo...High-entropy oxides(HEOs)are a new class of single-phase structures with unique electronic and catalytic properties;therefore,it is worthwhile to explore their applications in electrocatalysis.In this study,a Pt/(FeCoNiCrAl)_(3)O_(4)nanohybrid using HEO as a support was developed as an efficient catalyst for the hydrogen evolution reaction(HER).Pt/(FeCoNiCrAl)_(3)O_(4)exhibited high HER activity with a low overpotential of 22 mV at10 mA·cm^(-2),outperforming other binary,ternary,and quaternary supports.The HER activity of Pt/(FeCoNiCrAl)_(3)O_(4)was higher than that of a commercial Pt/C with a significantly lower Pt loading.The catalyst exhibited good activity and long-term stability(60 h)in an electrolytic water-splitting device.This good activity can be attributed to the fact that the introduction of Pt effectively facilitates electronic interactions between Pt and the HEO.In addition,the HEO substrate was more favorable for dispersing Pt particles,optimizing the electrochemical specific surface area,and significantly reducing the charge resistance of the HER.This study extends the application of HEOs in electrocatalysis and demonstrates the promising prospects of HEOs as supports for electrocatalysts.展开更多
基金supported by Hubei Natural Science Foundation Project(Grant No.2014CFB199)
文摘Objective: To determine the expression of non-muscle myosin heavy chain 9(MYH9) in osteosarcoma and its effect on the migration and invasion abilities of tumor cell. Methods: A total of 65 cases of osteosarcoma and 20 cases with benign osteochondroma who underwent resection operation in the Orthopaedics Department of our hospital from January 1st 2009 to January 1st 2015 were selected. Their mR NA levels of MYH9 were tested by q rt-PCR. Immunohistochemical method was used to examine the expression of MYH9 in osteosarcoma and the correlation between the positive expression of MYH9 and the clinicopathological features of patients was illustrated by statistical analysis. MYH9 was compounded artificially. The expression of MYH9 in SAOS2 osteosarcoma cells was decreased by si RNA. Scratch test was used to determine the change of SAOS2 cell migration ability after MYH9 silence. Transwell assay was employed to detect the change of cell invasion ability after MYH9 silence.Results: The expression levels of m RNA of MYH9 and protein in osteosarcoma tissues were significant higher than those in benign osteochondroma tissues. The high expression of MYH9 in osteosarcoma tissues was apparently related to the high Enneking classification(III classification) and lung metastasis. SiR NA of MYH9 could evidently decrease the expression level of MYH9 in SAOS2. The down-regulated expression of MYH9 could inhibit the migration and invasion abilities of SAOS2 cells. Conclusions: MYH9 shows a trend of high expression in osteosarcoma tissues, and its high expression is associated with features such as tumor invasion and metastasis. The down-regulated MYH9 can realize an anti-tumor effect by inhibiting the migration and invasion of osteosarcoma cells.
基金The application project of AIDS prevention and control of Ministry of health(WA2005-09)
文摘Objective:To get scientific basis for further health education through the research of the road construction workers'KBP before and after the interventions of highway AIDS prevention project.Methods:Multi-stage random sampling method was employeed to select workers of 8 sites from 14 sites along highway to investigate their AIDS knowledge,belief and performance(KBP)before and after highway AIDS prevention project.Results:Over 90%of the investigated workers had ever heard about AIDS,and the non-skilled workers of lower educational level improved more after intervention.The correct answer rate of the three transmitting ways of AIDS of drivers which is the focused group of highway before and after intervention had the obvious statistical significance(P<0.05),and the other group's correct answer rates also had improved after intervention.Most people's understanding of preventing AIDS through correct use of condoms when having sex had a statistically significanl differenee(P<0.05)after prevention.The rates of using condoms of foremen and skilled workers when having sex with commercial sex worker/casual partner increased after intervention.Conclusions:The health education of HIV among the road construction workers is effective and further health education of HIV prevention should be cairied out among the road construction workers to improve their knowledge and awareness of avoiding the high-risk behaviors.
基金financially supported by the National Natural Science Foundation of China(Nos.52122107,51972224 and 52001227)。
文摘High-entropy oxides(HEOs)are a new class of single-phase structures with unique electronic and catalytic properties;therefore,it is worthwhile to explore their applications in electrocatalysis.In this study,a Pt/(FeCoNiCrAl)_(3)O_(4)nanohybrid using HEO as a support was developed as an efficient catalyst for the hydrogen evolution reaction(HER).Pt/(FeCoNiCrAl)_(3)O_(4)exhibited high HER activity with a low overpotential of 22 mV at10 mA·cm^(-2),outperforming other binary,ternary,and quaternary supports.The HER activity of Pt/(FeCoNiCrAl)_(3)O_(4)was higher than that of a commercial Pt/C with a significantly lower Pt loading.The catalyst exhibited good activity and long-term stability(60 h)in an electrolytic water-splitting device.This good activity can be attributed to the fact that the introduction of Pt effectively facilitates electronic interactions between Pt and the HEO.In addition,the HEO substrate was more favorable for dispersing Pt particles,optimizing the electrochemical specific surface area,and significantly reducing the charge resistance of the HER.This study extends the application of HEOs in electrocatalysis and demonstrates the promising prospects of HEOs as supports for electrocatalysts.
