AIM:To investigate the association between the programmed death-1(PD-1)polymorphisms and genetic susceptibility of chronic hepatitis B virus(HBV)infection in Chinese patients.METHODS:Two single nucleotide polymorphism...AIM:To investigate the association between the programmed death-1(PD-1)polymorphisms and genetic susceptibility of chronic hepatitis B virus(HBV)infection in Chinese patients.METHODS:Two single nucleotide polymorphisms(SNPs),PD-1.1 G>A and PD-1.2 G>A,were genotyped in 539 patients with chronic HBV infection and 353 other family members(HbsAg-)from 256 nuclear families using polymerase chain reactiorestriction fragment length polymorphisms assay.The associations between PD-1 polymorphisms and genetic susceptibilityof chronic HBV infection were analyzed usng the familybased association analysis method.RESULTS:No association or linkage was detected among 539 patients.Univariate(single-marker)familybased association tests demonstrated that PD-1 genotypes,alleles and transmitted haplotypes are not associated with chronic HBV infection(all with P value more than 0.05).Transmission/disequilibrium test and sibship disequilibrium test analysis showed no excess of the alleles from heterozygous parents to affected offspring(P=0.688880,P=1.000000 respectively).CONCLUSION:The data demonstrated that PD-1.1 and PD-1.2 polymorphisms are not associated with chronic HBV infection in Chinese patients.展开更多
基金Supported by the Grants from the National Natural Science Foundation of China,No.30700698 and 30771907the Foundation of Pre-973 Program Projects,No.2009CB526411
文摘AIM:To investigate the association between the programmed death-1(PD-1)polymorphisms and genetic susceptibility of chronic hepatitis B virus(HBV)infection in Chinese patients.METHODS:Two single nucleotide polymorphisms(SNPs),PD-1.1 G>A and PD-1.2 G>A,were genotyped in 539 patients with chronic HBV infection and 353 other family members(HbsAg-)from 256 nuclear families using polymerase chain reactiorestriction fragment length polymorphisms assay.The associations between PD-1 polymorphisms and genetic susceptibilityof chronic HBV infection were analyzed usng the familybased association analysis method.RESULTS:No association or linkage was detected among 539 patients.Univariate(single-marker)familybased association tests demonstrated that PD-1 genotypes,alleles and transmitted haplotypes are not associated with chronic HBV infection(all with P value more than 0.05).Transmission/disequilibrium test and sibship disequilibrium test analysis showed no excess of the alleles from heterozygous parents to affected offspring(P=0.688880,P=1.000000 respectively).CONCLUSION:The data demonstrated that PD-1.1 and PD-1.2 polymorphisms are not associated with chronic HBV infection in Chinese patients.
