Segatella copri(formerly Prevotella copri)is a major indigestible polysaccharides consumer in the lower gut,and its interaction with polysaccharides is closely related to host health.To investigate the effects of S.co...Segatella copri(formerly Prevotella copri)is a major indigestible polysaccharides consumer in the lower gut,and its interaction with polysaccharides is closely related to host health.To investigate the effects of S.copriaccessible polysaccharides with different structures(SAPS)on gut microbiota and intestinal immunity,single strain and gut microbial fermentation were performed for accessibility testing,respectively,followed by cyclophosphamide(CTX)-induced immunocompromised mice experiment.During fermentation,xylan,arabinan,arabinoxylan,and arabinogalactan(four SAPS)promoted S.copri and gut microbiota growth with increased OD600 values,and enhanced the short-chain fatty acids(SCFAs)production,predominantly acetic acid.However,S.copri could not utilize xyloglucan.Interestingly,the four SAPS ameliorated CTX-induced immunocompromise with increased TNF-α,INF-γ,IL-6,IL-17,IL-2 in colon and index of spleen and thymus.Additionally,the four SAPS increased the relative abundance of Lactobacillus,Bacteroides,and S.copri,while inhibiting Oscillospira growth.Furthermore,they significantly promoted colonic levels of SCFAs,as well as altering the fecal metabolomic profile,e.g.Leukotriene C4,5,6-DHET,N-Methylserotonin and 24-Hydroxycholesterol,which were involved in arachidonic acid metabolism,tryptophan metabolism,and primary bile acid biosynthesis.Notably,arabinoxylan exhibited advantages in enhancing spleen index and MUC2 expression,whereas arabinan was more effective in increasing IL-17 and IL-6.Additionally,arabinogalactan increased colonic Claudin-1 expression,and xylan showed more benefits in promoting SCFAs production.In summary,the current research explored the regulation of gut microbiota and intestinal immunity by SAPS,providing a theoretical basis for the research on the relationship among polysaccharide structure,gut microbiota,and intestinal immunity.展开更多
基金support from National Science Fund for Excellent Young Scholars(32222065)National Key Research and Development Program of China(2023YFF1103600)+2 种基金Key Research and Development Project of Jiangxi Province(20223BBF61023)National Key Research and Development Program of China(2023YFF1105400)The Cen-tral Guidance Fund for Local Science and Technology Development in Jiangxi Province(20252ZDD020002)were gratefully acknowledged.
文摘Segatella copri(formerly Prevotella copri)is a major indigestible polysaccharides consumer in the lower gut,and its interaction with polysaccharides is closely related to host health.To investigate the effects of S.copriaccessible polysaccharides with different structures(SAPS)on gut microbiota and intestinal immunity,single strain and gut microbial fermentation were performed for accessibility testing,respectively,followed by cyclophosphamide(CTX)-induced immunocompromised mice experiment.During fermentation,xylan,arabinan,arabinoxylan,and arabinogalactan(four SAPS)promoted S.copri and gut microbiota growth with increased OD600 values,and enhanced the short-chain fatty acids(SCFAs)production,predominantly acetic acid.However,S.copri could not utilize xyloglucan.Interestingly,the four SAPS ameliorated CTX-induced immunocompromise with increased TNF-α,INF-γ,IL-6,IL-17,IL-2 in colon and index of spleen and thymus.Additionally,the four SAPS increased the relative abundance of Lactobacillus,Bacteroides,and S.copri,while inhibiting Oscillospira growth.Furthermore,they significantly promoted colonic levels of SCFAs,as well as altering the fecal metabolomic profile,e.g.Leukotriene C4,5,6-DHET,N-Methylserotonin and 24-Hydroxycholesterol,which were involved in arachidonic acid metabolism,tryptophan metabolism,and primary bile acid biosynthesis.Notably,arabinoxylan exhibited advantages in enhancing spleen index and MUC2 expression,whereas arabinan was more effective in increasing IL-17 and IL-6.Additionally,arabinogalactan increased colonic Claudin-1 expression,and xylan showed more benefits in promoting SCFAs production.In summary,the current research explored the regulation of gut microbiota and intestinal immunity by SAPS,providing a theoretical basis for the research on the relationship among polysaccharide structure,gut microbiota,and intestinal immunity.
