Background and Objectives:Non-Communicable Diseases(NCDs)represent a significant public health burden in China,particularly in cold regions where environmental factors further limit access to healthcare.To address thi...Background and Objectives:Non-Communicable Diseases(NCDs)represent a significant public health burden in China,particularly in cold regions where environmental factors further limit access to healthcare.To address this challenge,China has implemented the National Essential Public Health Service Package(NEPHSP)to improve NCD management through primary healthcare services.However,research on the effectiveness of NEPHSP in cold regions is limited.This study aims to identify the key factors influencing the implementation of NCD management services in cold regions,focusing on both barriers and facilitators.Methods:This qualitative study was conducted in three purposively selected cold regions in Northeast China(Harbin,Qiqihar,and Jiamusi).Data were collected through semi-structured interviews and focus group discussions,guided by the RE-AIM framework.Participants included local health administrators,primary healthcare workers,and community-dwelling individuals with NCDs.Thematic analysis was employed to code and extract key themes from the data.Results:A total of 72 participants(59.7%female,mean age 49.1±6.24 years)took part in the study.Major barriers to NCD management included low health literacy,limited awareness of NCDs,insufficient medical resources,poor public awareness,inadequate multisectoral collaboration,and the absence of effective audit and feedback systems.Key facilitators included the affordability and accessibility of primary healthcare services,strong patient-physician relationships,and the high priority given to NCD management by local governments.Furthermore,harsh environmental conditions and limited healthcare accessibility in cold regions complicated the management of NCDs,highlighting the need for tailored interventions that address the specific challenges of these regions.Conclusion:This study identifies significant facilitators and barriers to the implementation of NCD management services under the NEPHSP in cold regions of China.The unique challenges posed by cold climates necessitate the adaptation of healthcare services to local needs,with a focus on improving health literacy,enhancing healthcare infrastructure,and fostering multisectoral collaboration.These findings provide valuable insights for optimizing NCD prevention and management strategies within China's primary healthcare system.展开更多
AIM:To investigate the effects of gastric pacing on gastric emptying and plasma motilin level in a canine model of gastric motility disorders and the correlation between gastric emptying and plasma motilin level.METHO...AIM:To investigate the effects of gastric pacing on gastric emptying and plasma motilin level in a canine model of gastric motility disorders and the correlation between gastric emptying and plasma motilin level.METHODS: Ten healthy Mongrel dogs were divided into:experimental group of six dogs and control group of four dogs. A model of gastric motility disorders was established in the experimental group undergone truncal vagotomy combined with injection of glucagon. Gastric half-emptying time (GEt1/2) was monitored with single photon emission computerized tornography (SPECT), and the half-solid test meal was labeled with an isotope-^99mTc sulfur colloid. Plasma motilin concentration was measured with radioimmunoassay (RIA) kit. Surface gastric pacing at 1.1-1.2 times the intrinsic slow-wave frequency and a superimposed series of high frequency pulses (10-30Hz) was performed for 45min daily for a month in conscious dogs.RESULTS:After surgery, GEt1/2 in dogs undergone truncal vagotorny was increased significantly from 56.35±2.99min to 79.42±1.91min (P<0.001), but surface gastric pacing markedly accelerated gastric emptying and significantly decreased GEt1/2 to 64.94±1.75min (P<0.001) in animals undergone vagotomy. There was a significant increase of plasma level of motilin at the phase of IMCⅢ (interdigestive myoelectrical complex, IMCⅢ) in the dogs undergone bilateral truncal vagotomy (baseline vsvagotomy, 184.29±9.81pg/ml vs 242.09±17.22pg/ml;P<0.01).But plasma motilin concentration (212.55±11.20pg/ml; P<0.02) was decreased significantly after a long-term treatment with gastric pacing.Before gastric padng, GEt1/2 and plasma motilin concentration of the dogs undergone vagotomy showed a positive correlation (r=0.867, P<0.01), but after a long-term gastric pacing, GEt1/2 and motilin level showed a negative correlation (r=-0.733, P<0.04).CONCLUSION: Surface gastric pacing with optimal pacing parameters can improve gastric emptying parameters and significantly accelerate gastric emptying and can resume or alter motor function in a canine model of motility disorders. Gastric emptying is correlated well with plasma motilin level before and after padng, which suggests that motilin can modulate the mechanism of gastric pacing byaltering gastric motility.motilin can modulate the mechanism of gastric pacing by altering gastric motility.展开更多
Objective:To investigate the differential expression of microRNA(miRNA)in colon between ulcerative colitis(UC)and ulcerative colitis related colorectal cancer(UCRCC).Methods:An UC mouse model was built by dextran sodi...Objective:To investigate the differential expression of microRNA(miRNA)in colon between ulcerative colitis(UC)and ulcerative colitis related colorectal cancer(UCRCC).Methods:An UC mouse model was built by dextran sodium sulfate,and an UCRCC mouse model by dextran sodium sulfate and 1,2-diformylhydrazine.RNAs were extracted from the colon,purified and hybridized with fluorescence-labeled miRNA oligonucleotide gene chip.Real-time fluorescence quantitative PCR was used to verity the expression variation of miRNA.SAM was employed for the data analysis.Results:The up-regulated miRNA in colon cancer included has-miR-194,hasmiR-215,has-miR-93,has-miR-192,has-miR-92a,has-miR-29b,and has-miR-20a(median false discovery rate<5%),while the down-regulated miRNAs were has-miR-1231,has-miR-195,has-miR-143,and has-miR-145(median false discovery rate<5%).Conclusions:Significant differential expression of miRNA was found between the UC mouse and UCRCC mouse,which may be related to the onset,erosion and transfer of colorectal cancer.展开更多
Objective To study the two metal catalysts Ag/Al2O3 and Cu/Al2O3 that interdict the transmission pathway for SARS and other respiratory infectious diseases. Methods Two metal catalysts Ag/Al2O3 and Cu/Al2O3 were press...Objective To study the two metal catalysts Ag/Al2O3 and Cu/Al2O3 that interdict the transmission pathway for SARS and other respiratory infectious diseases. Methods Two metal catalysts Ag/Al2O3 and Cu/Al2O3 were pressed into wafers. One hundred μL 106 TCID50/mL SARS-CoV, 100 μL 106 PFU/mL recombinant baculovirus expressing hamster’s prion protein (haPrP) protein and roughly 106 E. coli were slowly dropped onto the surfaces of the catalyst wafers and exposed for 5 and 20 min, respectively. After eluted from the surfaces of wafers, the infectivity of viruses and propagation of bacteria were measured. The expression of PrP protein was determined by Western blot. The morphological changes of bacteria were observed by electronic microscopy. Results After exposure to the catalysts surfaces for 5 and 20 min, the infectivity of SARS-CoV in Vero cells and baculovirus in Sf9 cells dropped down to a very low and undetectable level, and no colony was detected using bacteria culture method. The expression of haPrP protein reduced to 21.8% in the preparation of Sf9 cells infected with recombinant baculovirus exposed for 5 min and was undetectable exposed for 20 min. Bacterial membranes seemed to be cracked and the cytoplasm seemed to be effluent from cell bodies. Conclusion Exposures to the surfaces of Ag/Al2O3 and Cu/Al2O3 destroy the replication and propagation abilities of SARS-CoV, baculovirus and E. coli. Inactivation ability of metal catalysts needs to interact with air, utilizing oxygen molecules in air. Efficiently killing viruses and bacteria on the surfaces of the two metal catalysts has a promising potential for air-disinfection in hospitals, communities, and households.展开更多
文摘Background and Objectives:Non-Communicable Diseases(NCDs)represent a significant public health burden in China,particularly in cold regions where environmental factors further limit access to healthcare.To address this challenge,China has implemented the National Essential Public Health Service Package(NEPHSP)to improve NCD management through primary healthcare services.However,research on the effectiveness of NEPHSP in cold regions is limited.This study aims to identify the key factors influencing the implementation of NCD management services in cold regions,focusing on both barriers and facilitators.Methods:This qualitative study was conducted in three purposively selected cold regions in Northeast China(Harbin,Qiqihar,and Jiamusi).Data were collected through semi-structured interviews and focus group discussions,guided by the RE-AIM framework.Participants included local health administrators,primary healthcare workers,and community-dwelling individuals with NCDs.Thematic analysis was employed to code and extract key themes from the data.Results:A total of 72 participants(59.7%female,mean age 49.1±6.24 years)took part in the study.Major barriers to NCD management included low health literacy,limited awareness of NCDs,insufficient medical resources,poor public awareness,inadequate multisectoral collaboration,and the absence of effective audit and feedback systems.Key facilitators included the affordability and accessibility of primary healthcare services,strong patient-physician relationships,and the high priority given to NCD management by local governments.Furthermore,harsh environmental conditions and limited healthcare accessibility in cold regions complicated the management of NCDs,highlighting the need for tailored interventions that address the specific challenges of these regions.Conclusion:This study identifies significant facilitators and barriers to the implementation of NCD management services under the NEPHSP in cold regions of China.The unique challenges posed by cold climates necessitate the adaptation of healthcare services to local needs,with a focus on improving health literacy,enhancing healthcare infrastructure,and fostering multisectoral collaboration.These findings provide valuable insights for optimizing NCD prevention and management strategies within China's primary healthcare system.
文摘AIM:To investigate the effects of gastric pacing on gastric emptying and plasma motilin level in a canine model of gastric motility disorders and the correlation between gastric emptying and plasma motilin level.METHODS: Ten healthy Mongrel dogs were divided into:experimental group of six dogs and control group of four dogs. A model of gastric motility disorders was established in the experimental group undergone truncal vagotomy combined with injection of glucagon. Gastric half-emptying time (GEt1/2) was monitored with single photon emission computerized tornography (SPECT), and the half-solid test meal was labeled with an isotope-^99mTc sulfur colloid. Plasma motilin concentration was measured with radioimmunoassay (RIA) kit. Surface gastric pacing at 1.1-1.2 times the intrinsic slow-wave frequency and a superimposed series of high frequency pulses (10-30Hz) was performed for 45min daily for a month in conscious dogs.RESULTS:After surgery, GEt1/2 in dogs undergone truncal vagotorny was increased significantly from 56.35±2.99min to 79.42±1.91min (P<0.001), but surface gastric pacing markedly accelerated gastric emptying and significantly decreased GEt1/2 to 64.94±1.75min (P<0.001) in animals undergone vagotomy. There was a significant increase of plasma level of motilin at the phase of IMCⅢ (interdigestive myoelectrical complex, IMCⅢ) in the dogs undergone bilateral truncal vagotomy (baseline vsvagotomy, 184.29±9.81pg/ml vs 242.09±17.22pg/ml;P<0.01).But plasma motilin concentration (212.55±11.20pg/ml; P<0.02) was decreased significantly after a long-term treatment with gastric pacing.Before gastric padng, GEt1/2 and plasma motilin concentration of the dogs undergone vagotomy showed a positive correlation (r=0.867, P<0.01), but after a long-term gastric pacing, GEt1/2 and motilin level showed a negative correlation (r=-0.733, P<0.04).CONCLUSION: Surface gastric pacing with optimal pacing parameters can improve gastric emptying parameters and significantly accelerate gastric emptying and can resume or alter motor function in a canine model of motility disorders. Gastric emptying is correlated well with plasma motilin level before and after padng, which suggests that motilin can modulate the mechanism of gastric pacing byaltering gastric motility.motilin can modulate the mechanism of gastric pacing by altering gastric motility.
基金funded by the Science and Technology Planning Project of Guangdong Province,China(NO.2011B031800214)
文摘Objective:To investigate the differential expression of microRNA(miRNA)in colon between ulcerative colitis(UC)and ulcerative colitis related colorectal cancer(UCRCC).Methods:An UC mouse model was built by dextran sodium sulfate,and an UCRCC mouse model by dextran sodium sulfate and 1,2-diformylhydrazine.RNAs were extracted from the colon,purified and hybridized with fluorescence-labeled miRNA oligonucleotide gene chip.Real-time fluorescence quantitative PCR was used to verity the expression variation of miRNA.SAM was employed for the data analysis.Results:The up-regulated miRNA in colon cancer included has-miR-194,hasmiR-215,has-miR-93,has-miR-192,has-miR-92a,has-miR-29b,and has-miR-20a(median false discovery rate<5%),while the down-regulated miRNAs were has-miR-1231,has-miR-195,has-miR-143,and has-miR-145(median false discovery rate<5%).Conclusions:Significant differential expression of miRNA was found between the UC mouse and UCRCC mouse,which may be related to the onset,erosion and transfer of colorectal cancer.
基金This work was supported by the National High-Technology Research and Development Program of China (863 Program) 2003AA208402 and2003AA208201.
文摘Objective To study the two metal catalysts Ag/Al2O3 and Cu/Al2O3 that interdict the transmission pathway for SARS and other respiratory infectious diseases. Methods Two metal catalysts Ag/Al2O3 and Cu/Al2O3 were pressed into wafers. One hundred μL 106 TCID50/mL SARS-CoV, 100 μL 106 PFU/mL recombinant baculovirus expressing hamster’s prion protein (haPrP) protein and roughly 106 E. coli were slowly dropped onto the surfaces of the catalyst wafers and exposed for 5 and 20 min, respectively. After eluted from the surfaces of wafers, the infectivity of viruses and propagation of bacteria were measured. The expression of PrP protein was determined by Western blot. The morphological changes of bacteria were observed by electronic microscopy. Results After exposure to the catalysts surfaces for 5 and 20 min, the infectivity of SARS-CoV in Vero cells and baculovirus in Sf9 cells dropped down to a very low and undetectable level, and no colony was detected using bacteria culture method. The expression of haPrP protein reduced to 21.8% in the preparation of Sf9 cells infected with recombinant baculovirus exposed for 5 min and was undetectable exposed for 20 min. Bacterial membranes seemed to be cracked and the cytoplasm seemed to be effluent from cell bodies. Conclusion Exposures to the surfaces of Ag/Al2O3 and Cu/Al2O3 destroy the replication and propagation abilities of SARS-CoV, baculovirus and E. coli. Inactivation ability of metal catalysts needs to interact with air, utilizing oxygen molecules in air. Efficiently killing viruses and bacteria on the surfaces of the two metal catalysts has a promising potential for air-disinfection in hospitals, communities, and households.
