Anti-aliasing spectrum analysis is essential for rotor blade condition monitoring based on Blade Tip Timing(BTT).The Multiple Signal Classification(MUSIC)algorithm,which exploits the orthogonality between signal and n...Anti-aliasing spectrum analysis is essential for rotor blade condition monitoring based on Blade Tip Timing(BTT).The Multiple Signal Classification(MUSIC)algorithm,which exploits the orthogonality between signal and noise subspaces,has been successfully applied for this purpose.However,conventional subspace selection methods relying on fixed thresholds are sensitive to variations in large eigenvalues.Furthermore,the complex disturbances during rotor operation and measurement complicate the identification of blade vibration characteristics.To overcome these challenges,this paper proposes Adaptive Subspace Separation(ASS)and Local Spectral Centroid(LSC)methods to improve the adaptability of subspace selection and the stability of frequency identification,respectively.The impacts of overestimating and underestimating the subspace dimensions on MUSIC's performance are derived mathematically.Simulation and experiments demonstrate the effectiveness of proposed approaches:ASS offers more accurate and stable subspace dimension selection and tracking,while LSC reduces the standard deviation of estimated frequencies by 30 percent.展开更多
[Objectives]To discuss the curative effect of the external application of self-prepared Chinese prescription decoction combined with gabapentin and cotton moxibustion on postherpetic neuralgia(PHN)and analyze its mech...[Objectives]To discuss the curative effect of the external application of self-prepared Chinese prescription decoction combined with gabapentin and cotton moxibustion on postherpetic neuralgia(PHN)and analyze its mechanism.[Methods]96 patients with PHN were divided into the control group(group A)and observation group(group B)(n=48).In group A,the patients were given gabapentin orally and treated by cotton moxibustion.In group B,the patients were treated by the external application of self-prepared Chinese prescription decoction on the basis of the above basic treatment.The total effective rate,apparent time and recurrence rate of the two groups were compared.[Results]The cure rate(72.91%)and total effective rate(95.83%)of patients in group B were higher than those in group A(54.16%,83.33%).The average apparent time[(5.79±1.40)d]and recurrence rate(5.97%)of patients in group B were significantly lower than those in group A[(7.03±4.37)d,10.45%],and the difference between the two groups was statistically significant(P<0.05).[Conclusions]The external application of self-prepared Chinese prescription decoction combined with gabapentin and cotton moxibustion therapy had a significant curative effect and low recurrence rate.展开更多
[Objectives]To observe the effects of Huanglian Jiedu Decoction on pain behaviors in rat models of postherpetic neuralgia(PHN)and analyze potential mechanisms.[Methods]Twenty SD rats with PHN models induced by intrapl...[Objectives]To observe the effects of Huanglian Jiedu Decoction on pain behaviors in rat models of postherpetic neuralgia(PHN)and analyze potential mechanisms.[Methods]Twenty SD rats with PHN models induced by intraplantar injection of 50μL(6×106)varicella-zoster virus(VZV)into the left hind paw were randomly divided into two groups(n=10 each)using a random number table:the model group(PHN group)and the Huanglian Jiedu Decoction group.From day 7 after inoculation,the Huanglian Jiedu Decoction group received oral gavage of Huanglian Jiedu Decoction twice daily for 14 days,while the PHN group received an equal volume of 0.9%saline.Thermal withdrawal latency(TWL)and mechanical withdrawal threshold(MWT)were measured at 1,4,7,14,and 21 d after inoculation.Pain-related behaviors(vocalization,scratching,and licking/biting of the hind paw)were recorded.[Results]Compared with the PHN group,the Huanglian Jiedu Decoction group exhibited significantly prolonged TWL and increased MWT at days 14 and 21(P<0.05).At day 4 post-inoculation,both groups showed marked pain-related behaviors(e.g.,vocalization,scratching,licking/biting).These behaviors persisted until day 21 in the PHN group but significantly decreased in the Huanglian Jiedu Decoction group at days 14 and 21(P<0.05).[Conclusions]Huanglian Jiedu Decoction reduces pain sensitivity and alleviates pain-related behaviors in PHN model rats.The mechanism may involve elevating pain thresholds and decreasing pain conduction velocity.展开更多
Xiaoer-Feire-Kechuan(XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation.The complicated composition rendered its chemical analysis and effective-component elucidation.In this study,w...Xiaoer-Feire-Kechuan(XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation.The complicated composition rendered its chemical analysis and effective-component elucidation.In this study,we combined quantitative analysis and bioactivity test to reveal the antiinflammatory constituents of XFK.First,UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 analytes(covering 9 out of 11 herbs) in different XFK formulations.Parallel reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and selectivity.Then,anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition assay.Finally,major analytes forsythosides H,I,A(8-10),and baicalin(15)(total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities(inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of XFK.The present study provided an effective strategy to discover effective constituents of multi-herb formulas.展开更多
Antrodia cinnamomea is a precious medicinal mushroom.It exhibits promising therapeutic effects on cancer,intoxication,hypertension,hepatitis,and inflammation.Its major bioactive constituents are ergostane and lanostan...Antrodia cinnamomea is a precious medicinal mushroom.It exhibits promising therapeutic effects on cancer,intoxication,hypertension,hepatitis,and inflammation.Its major bioactive constituents are ergostane and lanostane triterpenoids.In this study,we used intestinal Caco-2 cell monolayer model to reveal the intestinal absorption property of 14 representative triterpenoids from A.cinnamomea.The bidirectional transport through the monolayer at different time points was monitored by a fully validated LC/MS/MS method.In the case of pure compounds,ergostanes 5(25R-antcin H),6(25Santcin H)and 10(25R-antcin B)could readily pass through the Caco-2 cell layer,whereas lanostanes 13(dehydroeburicoic acid)and 14(eburicoic acid)could hardly pass through.When the cells were treated with A.cinnamomea extract,antcins A,B,C,H and K(1–6 and 9–11)were absorbed via passive transcellular diffusion,and showed high PAB and PBA values(>2.5×10^(-5) cm/s).Meanwhile,the lanostanes dehydrosulphurenic acid(8),15a-acetyldehydrosulphurenic acid(12),13 and 14 exhibited poor permeability.Transport features of these compounds were consistent with their pharmacokinetic behaviors in rats.This study could also be helpful in predicting the intestinal absorption of A.cinnamomea in human.展开更多
(+)-Sesamin was found in Semen Cuscutae for the first time. A rapid and simple approach for the analysis of(+)-sesamin in different sources of Semen Cuscutae is proposed, which used GC-FID for the determination of(+)-...(+)-Sesamin was found in Semen Cuscutae for the first time. A rapid and simple approach for the analysis of(+)-sesamin in different sources of Semen Cuscutae is proposed, which used GC-FID for the determination of(+)-sesamin and CC-MS for its identification. The result suggested that this approach could be used to identify Semen Cuscutae from various sources based on the different content of(+)-sesamin in them.展开更多
TongMai Granules is a Chinese medicine patent drug to treat cardiovascular diseases.It is prepared from the water extract of Dan-shen,Chuan-xiong and Ge-gen.The main compounds of TongMai Granules are isoflavone glycos...TongMai Granules is a Chinese medicine patent drug to treat cardiovascular diseases.It is prepared from the water extract of Dan-shen,Chuan-xiong and Ge-gen.The main compounds of TongMai Granules are isoflavone glycosides,isoflavones,and phenolic acids.However,it is difficult to detect low-polar compounds such as tanshinones and phthalides though they may also contribute to the therapeutic effect.In this work,an off-line two-dimensional chromatography method was developed to enrich the minor constituents in TongMai Granules.The extract was subjected to an SEP-PAK silica SPE column and eluted with petroleum ether,ethyl acetate–methanol(6:1,v/v),and methanol,successively.The three fractions were then analyzed using ultra-high performance liquid chromatography coupled with orbitrap mass spectrometry(UHPLC/orbitrap-MS).The isoflavones from Ge-gen,phenolic acids from Dan-shen and phthalides from Chuan-xiong were distributed in different fractions.A total of 131 compounds,including 59 from Ge-gen,26 from Dan-shen,43 from Chuan-xiong,and 3 common ones from Dan-shen and Chuan-xiong were tentatively identified.Among them,26 compounds were identified by comparing with reference standards.This study set a good example for comprehensive analysis of the constituents in traditional Chinese medicine formula.展开更多
Aluminum nitride （AlN） nanobelts were successfully synthesized in high yield through a chloride assisted vapor-solid process. X-ray diffraction, transmission electron microscopy, and selected area electronic diffrac...Aluminum nitride （AlN） nanobelts were successfully synthesized in high yield through a chloride assisted vapor-solid process. X-ray diffraction, transmission electron microscopy, and selected area electronic diffraction demonstrate that the as-prepared nanobelts are pure, structurally uniform and single crystalline, and can be indexed to hexagonal wurtzite structure. The micro observations show that there exist no defects in the obtained nanobelts. The growth direction of the nanobelts is along [0001]. The frequency spectra of the relative dielectric constant and of the dielectric loss were measured in the frequency range of 50 Hz to 5 MHz. Analysis of these spectra indicates that the interface in samples has great influence on the dielectric behavior of samples. As compared with AlN micropowders, AlN nanobelts have much higher relative dielectric constant, especially at low frequencies at room temperature.展开更多
[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment fr...[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment friendship. [Method] Using mung bean rooting method, the rooting promotion effects of 33 plant extracts on mung bean section were studied. [Result] When the concentration was 1 mg/mL, Eupatorium adenophorum （stem and leaf）, Cryptomeriafortunei （stem and leaf）, Pinus yunnanensis （stem）, Pinus yunnanensis （leaf）, Rhododendron simsii （leaf）, Pinus armandii （leaf）, Litchi chinensis （pericarp）, P/nus armandiifruit （fruit） and Alnus cremastogyne （leaf） showed strong promotion effect on rooting of mung bean sections, and the rooting promotion rate reached over 100% ; the roo- ting promotion effect ofE. adenophorum （stem and leaf） was the strongest, reaching（1068.42 ＋7.87）%, which was extremely higher than the extracts of other plants. [Conclusion] E. adenophorum （stem and leaf）, C. fortunei （stem and leaf）, P. yunnanens/s （stem）, P. yurmanens/s （leaf）, R. simsii （leaf）, P. armandii （leaf）, L. chinensis （pericarp）, P. armandiifruit （fruit） and A. cremastogyne （leaf） all contained substances with st/ong rooting promotion effect, which could be further used for development of natural rooting reagent.展开更多
Objective： Antrodia camphorata （AC）, a precious medicinal mushroom in Taiwan, is popularly used for adjuvant cancer therapy. This paper aims to clarify the metabolites which are present in tumor tissues after oral ...Objective： Antrodia camphorata （AC）, a precious medicinal mushroom in Taiwan, is popularly used for adjuvant cancer therapy. This paper aims to clarify the metabolites which are present in tumor tissues after oral administration of AC in Sarcoma-180 tumor-bearing mice, as well as their contents in tumors. Methods： Tumors of Sarcoma-180 tumor-bearing mice were obtained at 1 h and 4 h after oral administration of AC extract, and the metabolites in the tumor homogenate samples were characterized using UHPLC-orbitrap/MS analysis. Then, a fully validated LC-MS/MS method was developed for quantitative analysis of the most abundant compounds in tumor tissues, namely （25R/S）-antcin H. Results： A total of 33 compounds were characterized in tumor homogenate samples including 28 prototypes of triterpenoids and 5 metabolites. Among them, （25R）-antcin H and （25S）-antcin H had the highest contents of 2.03 and 0.66 μg/g tumor tissues for the 1 h group, and 2.04 and 0.59 μg/g tumor tissues for the 4 h group, respectively. It was obvious that （25R）-antcin H had higher tumor affinity than （25S）-antcin H, since the content of （25R）-antcin H was lower than that of （25S）-antcin H in AC extract （P 〈 0.01）. Conclusion： Triterpenoids can enter tumor tissues after oral administration of AC. Particularly, （25R）-antcin H showed higher exposure to tumor than （25S）-antcin H. These compounds could contribute to the anticancer activities of AC.展开更多
Currently,human health due to corona virus disease 2019(COVID-19)pandemic has been seriously threatened.The coronavirus severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)spike(S)protein plays a crucial role i...Currently,human health due to corona virus disease 2019(COVID-19)pandemic has been seriously threatened.The coronavirus severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)spike(S)protein plays a crucial role in virus transmission and several S-based therapeutic approaches have been approved for the treatment of COVID-19.However,the efficacy is compromised by the SARS-CoV-2 evolvement and mutation.Here we report the SARS-CoV-2 S protein receptor-binding domain(RBD)inhibitor licorice-saponin A3(A3)could widely inhibit RBD of SARS-CoV-2 variants,including Beta,Delta,and Omicron BA.1,XBB and BQ1.1.Furthermore,A3 could potently inhibit SARS-CoV-2 Omicron virus in Vero E6 cells,with EC50 of 1.016μM.The mechanism was related to binding with Y453 of RBD determined by hydrogen-deuterium exchange mass spectrometry(HDX-MS)analysis combined with quantum mechanics/molecular mechanics(QM/MM)simulations.Interestingly,phosphoproteomics analysis and multi fluorescent immunohistochemistry(mIHC)respectively indicated that A3 also inhibits host inflammation by directly modulating the JNK and p38 mitogen-activated protein kinase(MAPK)pathways and rebalancing the corresponding immune dysregulation.This work supports A3 as a promising broad-spectrum small molecule drug candidate for COVID-19.展开更多
Accurate aging diagnosis is crucial for the health and safety management of lithium-ion batteries in electric vehicles.Despite significant advancements achieved by data-driven methods,diagnosis accuracy remains constr...Accurate aging diagnosis is crucial for the health and safety management of lithium-ion batteries in electric vehicles.Despite significant advancements achieved by data-driven methods,diagnosis accuracy remains constrained by the high costs of check-up tests and the scarcity of labeled data.This paper presents a framework utilizing self-supervised machine learning to harness the potential of unlabeled data for diagnosing battery aging in electric vehicles during field operations.We validate our method using battery degradation datasets collected over more than two years from twenty real-world electric vehicles.Our analysis comprehensively addresses cell inconsistencies,physical interpretations,and charging uncertainties in real-world applications.This is achieved through self-supervised feature extraction using random short charging sequences in the main peak of incremental capacity curves.By leveraging inexpensive unlabeled data in a self-supervised approach,our method demonstrates improvements in average root mean square errors of 74.54%and 60.50%in the best and worst cases,respectively,compared to the supervised benchmark.This work underscores the potential of employing low-cost unlabeled data with self-supervised machine learning for effective battery health and safety management in realworld scenarios.展开更多
[Objectives]To explore the mechanism of Huanglian detoxification soup improving pain behavior by affecting serum IL-2 levels in postherpetic neuralgia(post herpes neuralgia,PHN)model rats.[Methods]20 PHN rats were ran...[Objectives]To explore the mechanism of Huanglian detoxification soup improving pain behavior by affecting serum IL-2 levels in postherpetic neuralgia(post herpes neuralgia,PHN)model rats.[Methods]20 PHN rats were randomly divided into model group(PHN group)and detoxification decoction group(n=10).In the group,10%Huanglian detoxification soup(0.4 mL/0.1 kg)was given once in the morning and evening for 14 d.The PHN group was filled with an equal volume of 0.9%sodium chloride solution.Tail vein serum inflammatory factor interleukin-2(IL-2),IL-βand IL-6 levels were measured using ELISA kits at 7 and 21 d.[Results]IL-2 increased at 14 and 21 d in IL group,while IL-βand IL-6 decreased compared with the PHN group(P<0.05).[Conclusions]Huanglian detoxification soup may raise IL-2 levels after VZV infection to promote the differentiation of cells of the immune system,so as to relieve the pain caused by IL-βand IL-6 inflammatory factors.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52405088 and 92360306)the Postdoctoral Fellowship Program of CPSF,China(No.GZC20241446)+2 种基金the Natural Science Basic Research Program of Shaanxi,China(No.2024JC-YBMS-402)the Fundamental Research Funds for the Central Universities,CHD(No.300102254102)the Foundation of Beilin District,China(No.GX2455)。
文摘Anti-aliasing spectrum analysis is essential for rotor blade condition monitoring based on Blade Tip Timing(BTT).The Multiple Signal Classification(MUSIC)algorithm,which exploits the orthogonality between signal and noise subspaces,has been successfully applied for this purpose.However,conventional subspace selection methods relying on fixed thresholds are sensitive to variations in large eigenvalues.Furthermore,the complex disturbances during rotor operation and measurement complicate the identification of blade vibration characteristics.To overcome these challenges,this paper proposes Adaptive Subspace Separation(ASS)and Local Spectral Centroid(LSC)methods to improve the adaptability of subspace selection and the stability of frequency identification,respectively.The impacts of overestimating and underestimating the subspace dimensions on MUSIC's performance are derived mathematically.Simulation and experiments demonstrate the effectiveness of proposed approaches:ASS offers more accurate and stable subspace dimension selection and tracking,while LSC reduces the standard deviation of estimated frequencies by 30 percent.
文摘[Objectives]To discuss the curative effect of the external application of self-prepared Chinese prescription decoction combined with gabapentin and cotton moxibustion on postherpetic neuralgia(PHN)and analyze its mechanism.[Methods]96 patients with PHN were divided into the control group(group A)and observation group(group B)(n=48).In group A,the patients were given gabapentin orally and treated by cotton moxibustion.In group B,the patients were treated by the external application of self-prepared Chinese prescription decoction on the basis of the above basic treatment.The total effective rate,apparent time and recurrence rate of the two groups were compared.[Results]The cure rate(72.91%)and total effective rate(95.83%)of patients in group B were higher than those in group A(54.16%,83.33%).The average apparent time[(5.79±1.40)d]and recurrence rate(5.97%)of patients in group B were significantly lower than those in group A[(7.03±4.37)d,10.45%],and the difference between the two groups was statistically significant(P<0.05).[Conclusions]The external application of self-prepared Chinese prescription decoction combined with gabapentin and cotton moxibustion therapy had a significant curative effect and low recurrence rate.
文摘[Objectives]To observe the effects of Huanglian Jiedu Decoction on pain behaviors in rat models of postherpetic neuralgia(PHN)and analyze potential mechanisms.[Methods]Twenty SD rats with PHN models induced by intraplantar injection of 50μL(6×106)varicella-zoster virus(VZV)into the left hind paw were randomly divided into two groups(n=10 each)using a random number table:the model group(PHN group)and the Huanglian Jiedu Decoction group.From day 7 after inoculation,the Huanglian Jiedu Decoction group received oral gavage of Huanglian Jiedu Decoction twice daily for 14 days,while the PHN group received an equal volume of 0.9%saline.Thermal withdrawal latency(TWL)and mechanical withdrawal threshold(MWT)were measured at 1,4,7,14,and 21 d after inoculation.Pain-related behaviors(vocalization,scratching,and licking/biting of the hind paw)were recorded.[Results]Compared with the PHN group,the Huanglian Jiedu Decoction group exhibited significantly prolonged TWL and increased MWT at days 14 and 21(P<0.05).At day 4 post-inoculation,both groups showed marked pain-related behaviors(e.g.,vocalization,scratching,licking/biting).These behaviors persisted until day 21 in the PHN group but significantly decreased in the Huanglian Jiedu Decoction group at days 14 and 21(P<0.05).[Conclusions]Huanglian Jiedu Decoction reduces pain sensitivity and alleviates pain-related behaviors in PHN model rats.The mechanism may involve elevating pain thresholds and decreasing pain conduction velocity.
基金supported by the National Key Research and Development Program of China (Grant No.: 2018YFC1707304, 2018YFC1707301)Beijing Natural Science Foundation (Grant No.: JQ18027)National Natural Science Foundation of China (Grant No.: 81725023)。
文摘Xiaoer-Feire-Kechuan(XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation.The complicated composition rendered its chemical analysis and effective-component elucidation.In this study,we combined quantitative analysis and bioactivity test to reveal the antiinflammatory constituents of XFK.First,UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 analytes(covering 9 out of 11 herbs) in different XFK formulations.Parallel reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and selectivity.Then,anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition assay.Finally,major analytes forsythosides H,I,A(8-10),and baicalin(15)(total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities(inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of XFK.The present study provided an effective strategy to discover effective constituents of multi-herb formulas.
基金This work was supported by National Natural Science Foundation of China(Nos.81222054,81303294)the Program for New Century Excellent Talents in University from Chinese Ministry of Education(No.NCET-11-0019).
文摘Antrodia cinnamomea is a precious medicinal mushroom.It exhibits promising therapeutic effects on cancer,intoxication,hypertension,hepatitis,and inflammation.Its major bioactive constituents are ergostane and lanostane triterpenoids.In this study,we used intestinal Caco-2 cell monolayer model to reveal the intestinal absorption property of 14 representative triterpenoids from A.cinnamomea.The bidirectional transport through the monolayer at different time points was monitored by a fully validated LC/MS/MS method.In the case of pure compounds,ergostanes 5(25R-antcin H),6(25Santcin H)and 10(25R-antcin B)could readily pass through the Caco-2 cell layer,whereas lanostanes 13(dehydroeburicoic acid)and 14(eburicoic acid)could hardly pass through.When the cells were treated with A.cinnamomea extract,antcins A,B,C,H and K(1–6 and 9–11)were absorbed via passive transcellular diffusion,and showed high PAB and PBA values(>2.5×10^(-5) cm/s).Meanwhile,the lanostanes dehydrosulphurenic acid(8),15a-acetyldehydrosulphurenic acid(12),13 and 14 exhibited poor permeability.Transport features of these compounds were consistent with their pharmacokinetic behaviors in rats.This study could also be helpful in predicting the intestinal absorption of A.cinnamomea in human.
基金NSFC, Grant !No. 29875001National ninth five-year Sci. & Tech. program of China, Grant !No. 96-903-02-02.
文摘(+)-Sesamin was found in Semen Cuscutae for the first time. A rapid and simple approach for the analysis of(+)-sesamin in different sources of Semen Cuscutae is proposed, which used GC-FID for the determination of(+)-sesamin and CC-MS for its identification. The result suggested that this approach could be used to identify Semen Cuscutae from various sources based on the different content of(+)-sesamin in them.
基金National Key Research and Development Program of China(Grant No.2018YFC1707301,2018YFC1707304)。
文摘TongMai Granules is a Chinese medicine patent drug to treat cardiovascular diseases.It is prepared from the water extract of Dan-shen,Chuan-xiong and Ge-gen.The main compounds of TongMai Granules are isoflavone glycosides,isoflavones,and phenolic acids.However,it is difficult to detect low-polar compounds such as tanshinones and phthalides though they may also contribute to the therapeutic effect.In this work,an off-line two-dimensional chromatography method was developed to enrich the minor constituents in TongMai Granules.The extract was subjected to an SEP-PAK silica SPE column and eluted with petroleum ether,ethyl acetate–methanol(6:1,v/v),and methanol,successively.The three fractions were then analyzed using ultra-high performance liquid chromatography coupled with orbitrap mass spectrometry(UHPLC/orbitrap-MS).The isoflavones from Ge-gen,phenolic acids from Dan-shen and phthalides from Chuan-xiong were distributed in different fractions.A total of 131 compounds,including 59 from Ge-gen,26 from Dan-shen,43 from Chuan-xiong,and 3 common ones from Dan-shen and Chuan-xiong were tentatively identified.Among them,26 compounds were identified by comparing with reference standards.This study set a good example for comprehensive analysis of the constituents in traditional Chinese medicine formula.
基金ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China （No.10674138 and No.20571022）. The authors express their appreciations to Professor Q. F. Fang, Dr. X. P. Wang, and Mr. Z. J. Cheng for the technical support and helpful discussions in the dielectric measurements.
文摘Aluminum nitride （AlN） nanobelts were successfully synthesized in high yield through a chloride assisted vapor-solid process. X-ray diffraction, transmission electron microscopy, and selected area electronic diffraction demonstrate that the as-prepared nanobelts are pure, structurally uniform and single crystalline, and can be indexed to hexagonal wurtzite structure. The micro observations show that there exist no defects in the obtained nanobelts. The growth direction of the nanobelts is along [0001]. The frequency spectra of the relative dielectric constant and of the dielectric loss were measured in the frequency range of 50 Hz to 5 MHz. Analysis of these spectra indicates that the interface in samples has great influence on the dielectric behavior of samples. As compared with AlN micropowders, AlN nanobelts have much higher relative dielectric constant, especially at low frequencies at room temperature.
基金Supported by The Natural Science Foundation Project of Yunnan Province ( No. 2010CD056)
文摘[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment friendship. [Method] Using mung bean rooting method, the rooting promotion effects of 33 plant extracts on mung bean section were studied. [Result] When the concentration was 1 mg/mL, Eupatorium adenophorum （stem and leaf）, Cryptomeriafortunei （stem and leaf）, Pinus yunnanensis （stem）, Pinus yunnanensis （leaf）, Rhododendron simsii （leaf）, Pinus armandii （leaf）, Litchi chinensis （pericarp）, P/nus armandiifruit （fruit） and Alnus cremastogyne （leaf） showed strong promotion effect on rooting of mung bean sections, and the rooting promotion rate reached over 100% ; the roo- ting promotion effect ofE. adenophorum （stem and leaf） was the strongest, reaching（1068.42 ＋7.87）%, which was extremely higher than the extracts of other plants. [Conclusion] E. adenophorum （stem and leaf）, C. fortunei （stem and leaf）, P. yunnanens/s （stem）, P. yurmanens/s （leaf）, R. simsii （leaf）, P. armandii （leaf）, L. chinensis （pericarp）, P. armandiifruit （fruit） and A. cremastogyne （leaf） all contained substances with st/ong rooting promotion effect, which could be further used for development of natural rooting reagent.
基金This work was supported by National Natural Science Foundation of China （No. 81303294）, the National Key Research and Development Program of China （No. 2017YFC1700405）, and Young Elite Scientists Sponsorship Program by CAST （2016QNRC001）.
文摘Objective： Antrodia camphorata （AC）, a precious medicinal mushroom in Taiwan, is popularly used for adjuvant cancer therapy. This paper aims to clarify the metabolites which are present in tumor tissues after oral administration of AC in Sarcoma-180 tumor-bearing mice, as well as their contents in tumors. Methods： Tumors of Sarcoma-180 tumor-bearing mice were obtained at 1 h and 4 h after oral administration of AC extract, and the metabolites in the tumor homogenate samples were characterized using UHPLC-orbitrap/MS analysis. Then, a fully validated LC-MS/MS method was developed for quantitative analysis of the most abundant compounds in tumor tissues, namely （25R/S）-antcin H. Results： A total of 33 compounds were characterized in tumor homogenate samples including 28 prototypes of triterpenoids and 5 metabolites. Among them, （25R）-antcin H and （25S）-antcin H had the highest contents of 2.03 and 0.66 μg/g tumor tissues for the 1 h group, and 2.04 and 0.59 μg/g tumor tissues for the 4 h group, respectively. It was obvious that （25R）-antcin H had higher tumor affinity than （25S）-antcin H, since the content of （25R）-antcin H was lower than that of （25S）-antcin H in AC extract （P 〈 0.01）. Conclusion： Triterpenoids can enter tumor tissues after oral administration of AC. Particularly, （25R）-antcin H showed higher exposure to tumor than （25S）-antcin H. These compounds could contribute to the anticancer activities of AC.
基金supported by National Natural Science Foundation of China(Grant Nos.:81891010/81891011,81725023,82003614,82173950,31770192,32070187,32161133003 and 82003681)China Postdoctoral Science Foundation(Grant No:2022T150029).
文摘Currently,human health due to corona virus disease 2019(COVID-19)pandemic has been seriously threatened.The coronavirus severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)spike(S)protein plays a crucial role in virus transmission and several S-based therapeutic approaches have been approved for the treatment of COVID-19.However,the efficacy is compromised by the SARS-CoV-2 evolvement and mutation.Here we report the SARS-CoV-2 S protein receptor-binding domain(RBD)inhibitor licorice-saponin A3(A3)could widely inhibit RBD of SARS-CoV-2 variants,including Beta,Delta,and Omicron BA.1,XBB and BQ1.1.Furthermore,A3 could potently inhibit SARS-CoV-2 Omicron virus in Vero E6 cells,with EC50 of 1.016μM.The mechanism was related to binding with Y453 of RBD determined by hydrogen-deuterium exchange mass spectrometry(HDX-MS)analysis combined with quantum mechanics/molecular mechanics(QM/MM)simulations.Interestingly,phosphoproteomics analysis and multi fluorescent immunohistochemistry(mIHC)respectively indicated that A3 also inhibits host inflammation by directly modulating the JNK and p38 mitogen-activated protein kinase(MAPK)pathways and rebalancing the corresponding immune dysregulation.This work supports A3 as a promising broad-spectrum small molecule drug candidate for COVID-19.
基金supported by the research project‘‘SafeDaBatt”(03EMF0409A)funded by the German Federal Ministry for Digital and Transport(BMDV)+2 种基金the National Key Research and Development Program of China(2022YFE0102700)the Key Research and Development Program of Shaanxi Province(2023-GHYB-05,2023-YBSF-104)the financial support from the China Scholarship Council(CSC)(202206567008)。
文摘Accurate aging diagnosis is crucial for the health and safety management of lithium-ion batteries in electric vehicles.Despite significant advancements achieved by data-driven methods,diagnosis accuracy remains constrained by the high costs of check-up tests and the scarcity of labeled data.This paper presents a framework utilizing self-supervised machine learning to harness the potential of unlabeled data for diagnosing battery aging in electric vehicles during field operations.We validate our method using battery degradation datasets collected over more than two years from twenty real-world electric vehicles.Our analysis comprehensively addresses cell inconsistencies,physical interpretations,and charging uncertainties in real-world applications.This is achieved through self-supervised feature extraction using random short charging sequences in the main peak of incremental capacity curves.By leveraging inexpensive unlabeled data in a self-supervised approach,our method demonstrates improvements in average root mean square errors of 74.54%and 60.50%in the best and worst cases,respectively,compared to the supervised benchmark.This work underscores the potential of employing low-cost unlabeled data with self-supervised machine learning for effective battery health and safety management in realworld scenarios.
文摘[Objectives]To explore the mechanism of Huanglian detoxification soup improving pain behavior by affecting serum IL-2 levels in postherpetic neuralgia(post herpes neuralgia,PHN)model rats.[Methods]20 PHN rats were randomly divided into model group(PHN group)and detoxification decoction group(n=10).In the group,10%Huanglian detoxification soup(0.4 mL/0.1 kg)was given once in the morning and evening for 14 d.The PHN group was filled with an equal volume of 0.9%sodium chloride solution.Tail vein serum inflammatory factor interleukin-2(IL-2),IL-βand IL-6 levels were measured using ELISA kits at 7 and 21 d.[Results]IL-2 increased at 14 and 21 d in IL group,while IL-βand IL-6 decreased compared with the PHN group(P<0.05).[Conclusions]Huanglian detoxification soup may raise IL-2 levels after VZV infection to promote the differentiation of cells of the immune system,so as to relieve the pain caused by IL-βand IL-6 inflammatory factors.
