Suitable temperature and light intensity play important roles in the formation of harmful algae blooms(HABs),which can pose serious threats to aquatic ecosystems and human health.In this study,we measured the growth,p...Suitable temperature and light intensity play important roles in the formation of harmful algae blooms(HABs),which can pose serious threats to aquatic ecosystems and human health.In this study,we measured the growth,physiological function,and paralytic shellfish toxins(PSTs)production of Alexandrium pacificum(CCMA-272),a strain isolated from East China Sea,at different temperatures(15,20,and 25℃)and light intensities(30,60,and 90μmol photons/(m^(2)·s)).Results indicate that temperature and light intensity significantly affected the growth,physiology,and toxigenic potentials of A.pacificum.The optimal conditions for the growth of A.pacificum were observed at 20℃ under60μmol photons/(m^(2)·s).Regarding the production of PSTs,this strain of A.pacificum produced 12 PSTs,including carbamate toxins:saxitoxin(STX),neosaxitoxin(NEO),and gonyautoxin 1–4(GTX1,GTX2,GTX3,GTX4);dicarbamoyl toxins:dicarbamoylsaxitoxin(dcSTX),dicarbamoylgonyautoxin 2,3(dcGTX2,dcGTX3);and N-sulfocarbamoyl toxins:N-sulfocarbamoylgonyautoxin 1,2(C1,C2),and gonyautoxin 5(GTX5).Among all the PSTs,C2 was the most abundant.Low temperature(15℃)and high light intensity(90μmol photons/(m^(2)·s))were beneficial for the production of PSTs in A.pacificum.When cultured at 20 and 25℃,A.pacificum generated comparable total quantities of PSTs,yet the toxicity levels were lower at 25℃.Intra-cellular PSTs contents were greater than extra-cellular PSTs contents,except those under the condition of 25℃ with 30μmol photons/(m^(2)·s).However,as the increase of temperature,A.pacificum released more amounts of analogues with higher toxicity levels(e.g.,STX and dcGTX_(2))into the environment than intracellularly.These findings emphasize the significant sensitivity of A.pacificum to temperature and light intensity,highlighting the importance of evaluating both intra-cellular and extra-cellular PSTs for assessing its toxicity and aiding in the prediction and management of HABs.展开更多
The data of field surveys during 2009 to 2018 was analyzed to understand the seasonality and inter-annual variability of the floating Ulva and Sargassum in the Subei Shoal,the southwestern Yellow Sea of China on decad...The data of field surveys during 2009 to 2018 was analyzed to understand the seasonality and inter-annual variability of the floating Ulva and Sargassum in the Subei Shoal,the southwestern Yellow Sea of China on decadal scale.The floating Ulva biomass was consistently originated from the central region of the Subei Shoal in middle to late April,increased rapidly,drifted and extended into the offshore water in May and June.The average floating Ulva biomass in the shoal generally increased over the years with evident inter-annual fluctuations.In contrast,pelagic Sargassum was accumulated in the Subei Shoal and formed the spring bloom only in 2013,2017 and 2018,and the biomass was higher than the co-occurring Ulva during the survey in these three years.Compared to the raft-origin floating Ulva,genesis and development of the pelagic Sargassum was distinct.Based on the current research,the Sargassum biomass was exotic and often initiated in the offshore water in March,and intruded into the shoal in April and May.The analysis on the environmental parameters was inconclusive since multiple anthropogenic and non-indigenous factors could influence the green tides in this region.Further research covering both the East China Sea and the Yellow Sea is needed to trace the origin of the floating Sargassum and to understand the interactions between these two co-occurring seaweeds.展开更多
Recently, the critical chain study has become a hot issue in the project management research field. The construction of the critical chain with multi-resource constraints is a new research subject. According to the sy...Recently, the critical chain study has become a hot issue in the project management research field. The construction of the critical chain with multi-resource constraints is a new research subject. According to the system analysis theory and project portfolio theory, this paper discusses the creation of project portfolios based on the similarity principle and gives the definition of priority in multi-resource allocation based on quantitative analysis. A model with multi-resource constraints, which can be applied to the critical chain construction of the A-bid section in the South-to-North Water Diversion Project, was proposed. Contrast analysis with the comprehensive treatment construction method and aggressive treatment construction method was carried out. This paper also makes suggestions for further research directions and subjects, which will be useful in improving the theories in relevant research fields.展开更多
BACKGROUND Heart failure(HF),especially HF with reduced ejection fraction(HFrEF),presents complex challenges,particularly in the aging population where it often coexists with type 2 diabetes mellitus(T2DM).AIM To anal...BACKGROUND Heart failure(HF),especially HF with reduced ejection fraction(HFrEF),presents complex challenges,particularly in the aging population where it often coexists with type 2 diabetes mellitus(T2DM).AIM To analyze the effect of dapagliflozin treatment on cardiac,renal function,and safety in patients with HFrEF combined with T2DM.METHODS Patients with T2DM complicated with HFrEF who underwent treatment in our hospital from February 2018 to March 2023 were retrospectively analyzed as the subjects of this study.The propensity score matching method was used,and a total of 102 eligible samples were scaled.The clinical efficacy of the two groups was evaluated at the end of the treatment,comparing the results of blood glucose,insulin,cardiac function,markers of myocardial injury,renal function indexes,and 6-min walk test(6MWT)before and after the treatment.We compared the occurrence of adverse effects on the treatment process of the two groups of patients.The incidence of adverse outcomes in patients within six months of treatment was counted.RESULTS The overall clinical efficacy rate of patients in the study group was significantly higher than that of patients in the control group(P=0.013).After treatment,the pancreatic beta-cell function index,left ventricular ejection fraction,and glomerular filtration rate of patients in the study group were significantly higher than control group(P<0.001),while their fasting plasma glucose,2-h postprandial glucose,glycosylated hemoglobin,insulin resistance index,left ventricular end-systolic diameter,left ventricular end-diastolic diameter,cardiac troponin I,creatine kinase-MB,N-terminal pro b-type natriuretic peptide,serum creatinine,and blood urea nitrogen were significantly lower than those of the control group.After treatment,patients in the study group had a significantly higher 6MWT than those in the control group(P<0.001).Hypoglycemic reaction(P=0.647),urinary tract infection(P=0.558),gastrointestinal adverse effect(P=0.307),respiratory disturbance(P=0.558),and angioedema(P=0.647)were not statistically different.There was no significant difference between the incidence of adverse outcomes between the two groups(P=0.250).CONCLUSION Dapagliflozin significantly enhances clinical efficacy,cardiac and renal function,and ambulatory capacity in patients with HFrEF and T2DM without an increased risk of adverse effects or outcomes.展开更多
A direct strategy for the creation of defects on carbon nanofibers (CNFs) has been developed by steam treatment.Nitrogen physisorption,XRD,Raman spectra,SEM and TEM analyses proved the existence of the new defects on ...A direct strategy for the creation of defects on carbon nanofibers (CNFs) has been developed by steam treatment.Nitrogen physisorption,XRD,Raman spectra,SEM and TEM analyses proved the existence of the new defects on CNFs.BET surface area of CNFs after steam treatment was enhanced from 20 to 378 m2/g.Pd catalysts supported on CNFs were also prepared by colloidal deposition method.The different activity of Pd/CNFs catalysts in the partial hydrogenation of phenylacetylene further demonstrated the diverse surfaces of CNFs could be formed by steam treatment.展开更多
[Objectives] To investigate the infrared identification of ZhuQin Formula and Atractylodes polysaccharides and its effect on immune function in immunocompromised mice. [Methods] In this experiment,the structure of pol...[Objectives] To investigate the infrared identification of ZhuQin Formula and Atractylodes polysaccharides and its effect on immune function in immunocompromised mice. [Methods] In this experiment,the structure of polysaccharides was analyzed by Fourier transform infrared spectroscopy. The experiment was divided into the polysaccharide groups of high,medium and low dose of ZhuQin,the extraction group of ZhuQin,the polysaccharide group of Atractylodes,the positive drug group,the blank group and the model group. The model of immunosuppressed mice was established by intraperitoneal injection of cyclophosphamide. The carbon clearance index,immune organ index,serum hemolysin,spleen T and B lymphocyte transformation rate were determined. [Results]The results show that the polysaccharides have obvious absorption peaks of polysaccharides; Formula extracts and polysaccharides can enhance the immune function of immunosuppressive mice at a certain dose level,and the effect of formula polysaccharide is better than that of extract and single traditional Chinese medicine polysaccharides. [Conclusions] The results suggest that the polysaccharides of ZhuQin Formula and Atractylodes may be acidic polysaccharides containing pyran ring and furan ring. ZhuQin Formula polysaccharide may play an immunomodulatory role mainly by improving the specific immunity of the organism.展开更多
Objective: To discuss the ultrasonic evaluation of fetal ventricular systolic function in hypertensive disorder complicating pregnancy and its correlation with the expression of placental hypoxia-related genes. Method...Objective: To discuss the ultrasonic evaluation of fetal ventricular systolic function in hypertensive disorder complicating pregnancy and its correlation with the expression of placental hypoxia-related genes. Methods: A total of 98 late pregnant women with hypertensive disorder complicating pregnancy who were treated in the hospital between December 2014 and February 2017 were selected as hypertensive disorder complicating pregnancy group and 100 normal late pregnant women who received physical examination in the hospital during the same period were selected as normal control group. The ultrasonic parameters of fetal ventricular systolic function in two groups of pregnant women were detected before delivery, and the expression of hypoxia-related genes in placental tissue was detected by fluorescence quantitative PCR. Pearson test was used to assess the correlation between the parameters of fetal ventricular systolic function and placental hypoxia in hypertensive disorder complicating pregnancy. Results: The fetal left ventricular fractional shortening and right ventricular fractional shortening levels of hypertensive disorder complicating pregnancy group were lower than those of normal control group. Angiogenesis-related genes VEGF, netrin-1 and XIAP mRNA expression in placental tissue of hypertensive disorder complicating pregnancy group were lower than those in placental tissue of normal control group while HIF-1α mRNA expression was higher than that in placental tissue of normal control group;oxidative stress-related genes Nrf2, TAC3 and PrxⅡ mRNA expression in placental tissue were lower than those in placental tissue of normal control group;apoptosis genes Fas, p53 and caspase-9 mRNA expression in placental tissue were higher than those in placental tissue of normal control group while Bcl-2 and SFRP2 mRNA expression were lower than those in placental tissue of normal control group. Pearson test showed that the ultrasonic parameter levels of fetal ventricular systolic function in hypertensive disorder complicating pregnancy were directly correlated with the degree of placental hypoxia. Conclusion:Ultrasonic testing of fetal ventricular systolic function in hypertensive disorder complicating pregnancy can be used as a reliable method to measure the degree of placental hypoxia.展开更多
OBJECTIVE:The objective is to explore the chest computed tomography(CT)and pulmonary function findings of coronavirus disease 2019(COVID‑19)survivors 1 month after discharge.METHODS:We retrospectively analyzed the che...OBJECTIVE:The objective is to explore the chest computed tomography(CT)and pulmonary function findings of coronavirus disease 2019(COVID‑19)survivors 1 month after discharge.METHODS:We retrospectively analyzed the chest CT and pulmonary function findings of 158(COVID‑19)survivors who were discharged and followed up at Wuhan Red Cross Hospital from April 9 to May 10,2020.RESULTS:Follow‑up chest CT revealed that pulmonary lesions in all patients were clearly absorbed and dissipated.One month after discharge,125(79.1%)patients had residual imaging abnormalities,mainly light ground‑glass opacities(44.9%)and light patchy shadows(38.6%).Other features included fibrous cord lesions in 90 cases(57.0%),pulmonary nodules in 46 cases(29.1%),pleural thickening in 44 cases(27.8%),and mediastinal lymphadenopathy in 81 cases(51.3%).Among 158 COVID‑19 survivors,124 were tested for pulmonary function.Pulmonary function tests revealed pulmonary function impairment in 10 patients(8.1%),restrictive ventilation dysfunction in 16(12.9%),and pulmonary diffusion dysfunction in 48(38.7%).Residual lung lesions and pleural thickening were negatively correlated with DLCO%(P<0.05).CONCLUSION:COVID‑19 patients have characteristic findings on chest CT and pulmonary function testing.Identifying these findings is helpful to dynamically monitor the disease prognosis.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.32101290,52009082)the Natural Science Foundation of Jiangsu Province(No.BK20210364)the Fundamental Research Funds for the Central Universities(No.B220202044)。
文摘Suitable temperature and light intensity play important roles in the formation of harmful algae blooms(HABs),which can pose serious threats to aquatic ecosystems and human health.In this study,we measured the growth,physiological function,and paralytic shellfish toxins(PSTs)production of Alexandrium pacificum(CCMA-272),a strain isolated from East China Sea,at different temperatures(15,20,and 25℃)and light intensities(30,60,and 90μmol photons/(m^(2)·s)).Results indicate that temperature and light intensity significantly affected the growth,physiology,and toxigenic potentials of A.pacificum.The optimal conditions for the growth of A.pacificum were observed at 20℃ under60μmol photons/(m^(2)·s).Regarding the production of PSTs,this strain of A.pacificum produced 12 PSTs,including carbamate toxins:saxitoxin(STX),neosaxitoxin(NEO),and gonyautoxin 1–4(GTX1,GTX2,GTX3,GTX4);dicarbamoyl toxins:dicarbamoylsaxitoxin(dcSTX),dicarbamoylgonyautoxin 2,3(dcGTX2,dcGTX3);and N-sulfocarbamoyl toxins:N-sulfocarbamoylgonyautoxin 1,2(C1,C2),and gonyautoxin 5(GTX5).Among all the PSTs,C2 was the most abundant.Low temperature(15℃)and high light intensity(90μmol photons/(m^(2)·s))were beneficial for the production of PSTs in A.pacificum.When cultured at 20 and 25℃,A.pacificum generated comparable total quantities of PSTs,yet the toxicity levels were lower at 25℃.Intra-cellular PSTs contents were greater than extra-cellular PSTs contents,except those under the condition of 25℃ with 30μmol photons/(m^(2)·s).However,as the increase of temperature,A.pacificum released more amounts of analogues with higher toxicity levels(e.g.,STX and dcGTX_(2))into the environment than intracellularly.These findings emphasize the significant sensitivity of A.pacificum to temperature and light intensity,highlighting the importance of evaluating both intra-cellular and extra-cellular PSTs for assessing its toxicity and aiding in the prediction and management of HABs.
基金Foundation item:The National Key Research and Development Program of China under contract No.2016YFC1402100the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2018SDKJ0505-4+1 种基金the National Natural Science Foundation of China under contract No.41876137the NSFC-Shandong Joint Funded Project under contract No.U1606404.
文摘The data of field surveys during 2009 to 2018 was analyzed to understand the seasonality and inter-annual variability of the floating Ulva and Sargassum in the Subei Shoal,the southwestern Yellow Sea of China on decadal scale.The floating Ulva biomass was consistently originated from the central region of the Subei Shoal in middle to late April,increased rapidly,drifted and extended into the offshore water in May and June.The average floating Ulva biomass in the shoal generally increased over the years with evident inter-annual fluctuations.In contrast,pelagic Sargassum was accumulated in the Subei Shoal and formed the spring bloom only in 2013,2017 and 2018,and the biomass was higher than the co-occurring Ulva during the survey in these three years.Compared to the raft-origin floating Ulva,genesis and development of the pelagic Sargassum was distinct.Based on the current research,the Sargassum biomass was exotic and often initiated in the offshore water in March,and intruded into the shoal in April and May.The analysis on the environmental parameters was inconclusive since multiple anthropogenic and non-indigenous factors could influence the green tides in this region.Further research covering both the East China Sea and the Yellow Sea is needed to trace the origin of the floating Sargassum and to understand the interactions between these two co-occurring seaweeds.
基金supported by the National Science and Technology Plan (Major Project of the Eleventh Five-Year Plan,Grant No. 2006BAB04A13)the Philosophy and Social Science Fund of the Education Department of Jiangsu Province (Grant No.07SJD630006)+2 种基金the Third Key Discipline (Techno-Economics and Management) of the 211 Projectthe Key Discipline of Jiangsu Province (Engineering and Project Management)the Office of the South-to-North Water Diversion Project Construction Committee under the State Council
文摘Recently, the critical chain study has become a hot issue in the project management research field. The construction of the critical chain with multi-resource constraints is a new research subject. According to the system analysis theory and project portfolio theory, this paper discusses the creation of project portfolios based on the similarity principle and gives the definition of priority in multi-resource allocation based on quantitative analysis. A model with multi-resource constraints, which can be applied to the critical chain construction of the A-bid section in the South-to-North Water Diversion Project, was proposed. Contrast analysis with the comprehensive treatment construction method and aggressive treatment construction method was carried out. This paper also makes suggestions for further research directions and subjects, which will be useful in improving the theories in relevant research fields.
文摘BACKGROUND Heart failure(HF),especially HF with reduced ejection fraction(HFrEF),presents complex challenges,particularly in the aging population where it often coexists with type 2 diabetes mellitus(T2DM).AIM To analyze the effect of dapagliflozin treatment on cardiac,renal function,and safety in patients with HFrEF combined with T2DM.METHODS Patients with T2DM complicated with HFrEF who underwent treatment in our hospital from February 2018 to March 2023 were retrospectively analyzed as the subjects of this study.The propensity score matching method was used,and a total of 102 eligible samples were scaled.The clinical efficacy of the two groups was evaluated at the end of the treatment,comparing the results of blood glucose,insulin,cardiac function,markers of myocardial injury,renal function indexes,and 6-min walk test(6MWT)before and after the treatment.We compared the occurrence of adverse effects on the treatment process of the two groups of patients.The incidence of adverse outcomes in patients within six months of treatment was counted.RESULTS The overall clinical efficacy rate of patients in the study group was significantly higher than that of patients in the control group(P=0.013).After treatment,the pancreatic beta-cell function index,left ventricular ejection fraction,and glomerular filtration rate of patients in the study group were significantly higher than control group(P<0.001),while their fasting plasma glucose,2-h postprandial glucose,glycosylated hemoglobin,insulin resistance index,left ventricular end-systolic diameter,left ventricular end-diastolic diameter,cardiac troponin I,creatine kinase-MB,N-terminal pro b-type natriuretic peptide,serum creatinine,and blood urea nitrogen were significantly lower than those of the control group.After treatment,patients in the study group had a significantly higher 6MWT than those in the control group(P<0.001).Hypoglycemic reaction(P=0.647),urinary tract infection(P=0.558),gastrointestinal adverse effect(P=0.307),respiratory disturbance(P=0.558),and angioedema(P=0.647)were not statistically different.There was no significant difference between the incidence of adverse outcomes between the two groups(P=0.250).CONCLUSION Dapagliflozin significantly enhances clinical efficacy,cardiac and renal function,and ambulatory capacity in patients with HFrEF and T2DM without an increased risk of adverse effects or outcomes.
基金supported by the National Natural Science Foundation of China(21073023 and 20906008)the Fundamental Research Funds for the Central Universities(DUT12YQ03)the CSC and DAAD for a Project Based Personnel Exchange Program
文摘A direct strategy for the creation of defects on carbon nanofibers (CNFs) has been developed by steam treatment.Nitrogen physisorption,XRD,Raman spectra,SEM and TEM analyses proved the existence of the new defects on CNFs.BET surface area of CNFs after steam treatment was enhanced from 20 to 378 m2/g.Pd catalysts supported on CNFs were also prepared by colloidal deposition method.The different activity of Pd/CNFs catalysts in the partial hydrogenation of phenylacetylene further demonstrated the diverse surfaces of CNFs could be formed by steam treatment.
基金Supported by Public Welfare and Industry Special Fund Project of the Ministry of Agriculture(201303040-05)Natural Science Foundation Project of CQCSTC(2013FYF110600)
文摘[Objectives] To investigate the infrared identification of ZhuQin Formula and Atractylodes polysaccharides and its effect on immune function in immunocompromised mice. [Methods] In this experiment,the structure of polysaccharides was analyzed by Fourier transform infrared spectroscopy. The experiment was divided into the polysaccharide groups of high,medium and low dose of ZhuQin,the extraction group of ZhuQin,the polysaccharide group of Atractylodes,the positive drug group,the blank group and the model group. The model of immunosuppressed mice was established by intraperitoneal injection of cyclophosphamide. The carbon clearance index,immune organ index,serum hemolysin,spleen T and B lymphocyte transformation rate were determined. [Results]The results show that the polysaccharides have obvious absorption peaks of polysaccharides; Formula extracts and polysaccharides can enhance the immune function of immunosuppressive mice at a certain dose level,and the effect of formula polysaccharide is better than that of extract and single traditional Chinese medicine polysaccharides. [Conclusions] The results suggest that the polysaccharides of ZhuQin Formula and Atractylodes may be acidic polysaccharides containing pyran ring and furan ring. ZhuQin Formula polysaccharide may play an immunomodulatory role mainly by improving the specific immunity of the organism.
文摘Objective: To discuss the ultrasonic evaluation of fetal ventricular systolic function in hypertensive disorder complicating pregnancy and its correlation with the expression of placental hypoxia-related genes. Methods: A total of 98 late pregnant women with hypertensive disorder complicating pregnancy who were treated in the hospital between December 2014 and February 2017 were selected as hypertensive disorder complicating pregnancy group and 100 normal late pregnant women who received physical examination in the hospital during the same period were selected as normal control group. The ultrasonic parameters of fetal ventricular systolic function in two groups of pregnant women were detected before delivery, and the expression of hypoxia-related genes in placental tissue was detected by fluorescence quantitative PCR. Pearson test was used to assess the correlation between the parameters of fetal ventricular systolic function and placental hypoxia in hypertensive disorder complicating pregnancy. Results: The fetal left ventricular fractional shortening and right ventricular fractional shortening levels of hypertensive disorder complicating pregnancy group were lower than those of normal control group. Angiogenesis-related genes VEGF, netrin-1 and XIAP mRNA expression in placental tissue of hypertensive disorder complicating pregnancy group were lower than those in placental tissue of normal control group while HIF-1α mRNA expression was higher than that in placental tissue of normal control group;oxidative stress-related genes Nrf2, TAC3 and PrxⅡ mRNA expression in placental tissue were lower than those in placental tissue of normal control group;apoptosis genes Fas, p53 and caspase-9 mRNA expression in placental tissue were higher than those in placental tissue of normal control group while Bcl-2 and SFRP2 mRNA expression were lower than those in placental tissue of normal control group. Pearson test showed that the ultrasonic parameter levels of fetal ventricular systolic function in hypertensive disorder complicating pregnancy were directly correlated with the degree of placental hypoxia. Conclusion:Ultrasonic testing of fetal ventricular systolic function in hypertensive disorder complicating pregnancy can be used as a reliable method to measure the degree of placental hypoxia.
基金partly supported by operating research grants from the Sichuan Provincial Program for Diagnostic and Treatment of COVID-19(grant no.2020YFS0002)the Science and Technology Project of Sichuan Province(grant no.2020YFS0572)the Nanchong Science and Technology Bureau project(grant no.20YFZJ0105).
文摘OBJECTIVE:The objective is to explore the chest computed tomography(CT)and pulmonary function findings of coronavirus disease 2019(COVID‑19)survivors 1 month after discharge.METHODS:We retrospectively analyzed the chest CT and pulmonary function findings of 158(COVID‑19)survivors who were discharged and followed up at Wuhan Red Cross Hospital from April 9 to May 10,2020.RESULTS:Follow‑up chest CT revealed that pulmonary lesions in all patients were clearly absorbed and dissipated.One month after discharge,125(79.1%)patients had residual imaging abnormalities,mainly light ground‑glass opacities(44.9%)and light patchy shadows(38.6%).Other features included fibrous cord lesions in 90 cases(57.0%),pulmonary nodules in 46 cases(29.1%),pleural thickening in 44 cases(27.8%),and mediastinal lymphadenopathy in 81 cases(51.3%).Among 158 COVID‑19 survivors,124 were tested for pulmonary function.Pulmonary function tests revealed pulmonary function impairment in 10 patients(8.1%),restrictive ventilation dysfunction in 16(12.9%),and pulmonary diffusion dysfunction in 48(38.7%).Residual lung lesions and pleural thickening were negatively correlated with DLCO%(P<0.05).CONCLUSION:COVID‑19 patients have characteristic findings on chest CT and pulmonary function testing.Identifying these findings is helpful to dynamically monitor the disease prognosis.
