Abstract： A new sulfated triterpene glycoside named as 17-dehydroxyholothurin A 1 was isolated from the sea cucumber Holothuria impatiens. Its structure was elucidated on the basis of spectroscopic data （2D NMR and...Abstract： A new sulfated triterpene glycoside named as 17-dehydroxyholothurin A 1 was isolated from the sea cucumber Holothuria impatiens. Its structure was elucidated on the basis of spectroscopic data （2D NMR and MS） and chemical evidence. Compound 1 showed cytotoxic activity against cancer cell lines MKN-28, MFC-7, KB, HL-60 and Hep G2 with IC50 values of 1.98, 4.53, 2.01, 4.69 and 2.80 ×10^-6 tool/L, respectively.展开更多
In this paper,the aero-engine test with inter-shaft bearing fault is carried out,and a dataset is proposed for the first time based on the vibration signal of rotors and casings.First,a test rig based on a real aero-e...In this paper,the aero-engine test with inter-shaft bearing fault is carried out,and a dataset is proposed for the first time based on the vibration signal of rotors and casings.First,a test rig based on a real aero-engine is established,driven by motors and equipped with a lubricating system.Then,the aero-engine is disassembled and assembled following the specification process,and the inter-shaft bearing with artificial fault is replaced.Next,the aero-engine test is conducted at 28 groups of high-and low-pressure speeds.Six measuring points are arranged,including two displacement sensors to test the displacement vibration signals of the low-pressure rotor and four acceleration sensors to test the acceleration vibration signals of the casing.The test results are integrated into an inter-shaft bearing fault dataset.Finally,based on the dataset in this paper,frequency spectrum,envelope spectrum,CNN,LSTM,and TST are used for fault diagnosis,and the results are compared with those of CWRU and XJTU datasets.The results show that the characteristic fault frequency cannot be found directly in the spectrum and envelope spectrum corresponding to this paper’s dataset but in CWRU and XJTU datasets.Using CNN,LSTM,and TST for fault diagnosis of the dataset in this paper,the accuracy is 83.13%,85.41%,and 71.07%,respectively,much lower than the diagnosis results of CWRU and XJTU datasets.It can be seen that the dataset in this paper is closer to the actual fault diagnosis situation and is a more challenging dataset.This dataset provides a new benchmark for the validation of fault diagnosis methods.Mendeley data:https://github.com/HouLeiHIT/HIT-dataset.展开更多
In this study,wild eggplant germplasm No.M239,which is highly susceptible to Verticillium wilt,was used as the experimental material.The physiological and biochemical indices(SOD,PAL,MDA and soluble protein)of M239 ro...In this study,wild eggplant germplasm No.M239,which is highly susceptible to Verticillium wilt,was used as the experimental material.The physiological and biochemical indices(SOD,PAL,MDA and soluble protein)of M239 roots were measured at different times(0,12,24,36,48,60 and 72 h)post inoculation with Verticillium dahliae,and the key time points for the M239 response to Verticillium wilt infection were screened.Then,RNA-Seq technology was used to screen the differentially expressed genes(DEGs)in M239 roots at 0,12 and 48 h post-inoculation(hpi).The transcriptional results of M239 were also compared with those resistance genes from some reported wild relative Solanum species(S.sisymbriifolium and S.aculeatissimum).Then some DEGs were chosen for validation by qRT–PCR.The results showed that 12 and 48 hpi were the turning points in the changes in all physiological and biochemical indices.A total of 6,783 DEGs were identified by RNA-Seq,including 6,141 DEGs(3,046 upregulated and 3,095 downregulated)at the M_12 h vs.M_0 h,1,903 DEGs(792 upregulated and 1,111 downregulated)at M_48 h vs.M_12 h,and 1,261 DEGs that appeared simultaneously in both stages.KEGG enrichment analysis showed that there were 5 metabolic pathways enriched from DEGs,which were mostly related to primary metabolism,such as glycolysis,amino acid and ribosome biogenesis.Compared with the NCBI non-redundant protein(NR)database,one Ve2 homologous gene and 8 PR protein-related genes were screened.Transcription factor analysis showed that there were a large number of DEGs,such as MYB,AP2-EREBP,bHLH,NAC and Orphans in the two stages.Compared with the reported Verticillium wilt-resistant wild eggplant species,it was found that there were fewer genes and enriched metabolic pathways in the M239 response to Verticillium wilt infection,and it also lacked the response of some known key resistance genes.These results proved that the above resistance genes and metabolic pathways played a key role in the wild eggplant response to V.dahliae infection.展开更多
文摘Abstract： A new sulfated triterpene glycoside named as 17-dehydroxyholothurin A 1 was isolated from the sea cucumber Holothuria impatiens. Its structure was elucidated on the basis of spectroscopic data （2D NMR and MS） and chemical evidence. Compound 1 showed cytotoxic activity against cancer cell lines MKN-28, MFC-7, KB, HL-60 and Hep G2 with IC50 values of 1.98, 4.53, 2.01, 4.69 and 2.80 ×10^-6 tool/L, respectively.
基金supports from the National Natural Science Foundation of China (Grant No.11972129)the Natural Science Foundation of Heilongjiang Province (Outstanding Youth Foundation,Grant No.YQ2022A008)the Fundamental Research Funds for the Central Universities.
文摘In this paper,the aero-engine test with inter-shaft bearing fault is carried out,and a dataset is proposed for the first time based on the vibration signal of rotors and casings.First,a test rig based on a real aero-engine is established,driven by motors and equipped with a lubricating system.Then,the aero-engine is disassembled and assembled following the specification process,and the inter-shaft bearing with artificial fault is replaced.Next,the aero-engine test is conducted at 28 groups of high-and low-pressure speeds.Six measuring points are arranged,including two displacement sensors to test the displacement vibration signals of the low-pressure rotor and four acceleration sensors to test the acceleration vibration signals of the casing.The test results are integrated into an inter-shaft bearing fault dataset.Finally,based on the dataset in this paper,frequency spectrum,envelope spectrum,CNN,LSTM,and TST are used for fault diagnosis,and the results are compared with those of CWRU and XJTU datasets.The results show that the characteristic fault frequency cannot be found directly in the spectrum and envelope spectrum corresponding to this paper’s dataset but in CWRU and XJTU datasets.Using CNN,LSTM,and TST for fault diagnosis of the dataset in this paper,the accuracy is 83.13%,85.41%,and 71.07%,respectively,much lower than the diagnosis results of CWRU and XJTU datasets.It can be seen that the dataset in this paper is closer to the actual fault diagnosis situation and is a more challenging dataset.This dataset provides a new benchmark for the validation of fault diagnosis methods.Mendeley data:https://github.com/HouLeiHIT/HIT-dataset.
基金supported by the National Natural Science Foundation of China(31960594)the Yunnan Fundamental Research Projects(202201AT070074,2019FB059)the Yunnan Technical Innovation Talent Training Project(202205AD160029).
文摘In this study,wild eggplant germplasm No.M239,which is highly susceptible to Verticillium wilt,was used as the experimental material.The physiological and biochemical indices(SOD,PAL,MDA and soluble protein)of M239 roots were measured at different times(0,12,24,36,48,60 and 72 h)post inoculation with Verticillium dahliae,and the key time points for the M239 response to Verticillium wilt infection were screened.Then,RNA-Seq technology was used to screen the differentially expressed genes(DEGs)in M239 roots at 0,12 and 48 h post-inoculation(hpi).The transcriptional results of M239 were also compared with those resistance genes from some reported wild relative Solanum species(S.sisymbriifolium and S.aculeatissimum).Then some DEGs were chosen for validation by qRT–PCR.The results showed that 12 and 48 hpi were the turning points in the changes in all physiological and biochemical indices.A total of 6,783 DEGs were identified by RNA-Seq,including 6,141 DEGs(3,046 upregulated and 3,095 downregulated)at the M_12 h vs.M_0 h,1,903 DEGs(792 upregulated and 1,111 downregulated)at M_48 h vs.M_12 h,and 1,261 DEGs that appeared simultaneously in both stages.KEGG enrichment analysis showed that there were 5 metabolic pathways enriched from DEGs,which were mostly related to primary metabolism,such as glycolysis,amino acid and ribosome biogenesis.Compared with the NCBI non-redundant protein(NR)database,one Ve2 homologous gene and 8 PR protein-related genes were screened.Transcription factor analysis showed that there were a large number of DEGs,such as MYB,AP2-EREBP,bHLH,NAC and Orphans in the two stages.Compared with the reported Verticillium wilt-resistant wild eggplant species,it was found that there were fewer genes and enriched metabolic pathways in the M239 response to Verticillium wilt infection,and it also lacked the response of some known key resistance genes.These results proved that the above resistance genes and metabolic pathways played a key role in the wild eggplant response to V.dahliae infection.
