The epidermal growth factor(EGF)has been shown to promote the proliferation of various types of cells,to maintain the physiological function of the mucosa of the digestive tract,and to promote the healing of the gastr...The epidermal growth factor(EGF)has been shown to promote the proliferation of various types of cells,to maintain the physiological function of the mucosa of the digestive tract,and to promote the healing of the gastric and duodenal ulcers.It has been expressed in many types of bacteria and yeasts.In this article,a bio-reactor was constructed,namely,the human EGF(hEGF)transgenic mini-tomato.On the basis of hEGF gene sequence,a tomato codon preference hEGF gene was chemically synthesized,and it was constructed into the plant expression vector pCAMBIA2300.The transgenic tomato plants containing gene hEGF were obtained through Agrobacterium-mediated transformation.The expression product was determined by radioimmunoassay(RIA)and showed a yield of 3.48±1.01 ng/g fresh fruits.The intragastric gavage(ig)administration of the rhEGF-containing juice of the transgenic tomato(equivalent to 24 ng rhEGF per mouse a day)for 15 days could significantly protect mice against the alcohol-induced ulceration.The ulcer index,expressed as a degree of the stomach lesion,decreased from 42.20±18.13 to 16.25±9.57.展开更多
In this editorial,we have taken an in-depth look at the article published by Wan et al.The study showed that preconditioning mesenchymal stem cells(MSCs)protected them against programmed cell death,and increased their...In this editorial,we have taken an in-depth look at the article published by Wan et al.The study showed that preconditioning mesenchymal stem cells(MSCs)protected them against programmed cell death,and increased their survival rate and therapeutic potential.Autophagy,a type of programmed cell death,is a major intracellular degradation and recycling pathway that is crucial for maintaining cellular homeostasis,self-renewal,and pluripotency.We have explored the relationship between autophagy and MSCs to determine the role of autophagy in the therapeutic applications of MSCs.展开更多
A series of 2D direct numerical simulations were performed with an accurate level set method for single drop impacts.The adopted ACLS method was validated to be efficient with perfect mass conservation in both normal ...A series of 2D direct numerical simulations were performed with an accurate level set method for single drop impacts.The adopted ACLS method was validated to be efficient with perfect mass conservation in both normal and oblique impacts.A square-root correction for neck bases was modified in accuracy as well as scope of applications.In addition,process of jet formation and evolution was studied to reveal internal dynamics in drop impacts.It's found that pressure gradient and vortex are coexisting and completive reasons for jet topology while the inclined angle has a significant effect on them.Mechanisms of jet formation and evolution are different in the front and back necks.With the help of PDF distribution and correction calculation,a compromise in the competition is observed.This work lays a solid foundation for further studies of dynamics in gas-liquid flows.展开更多
[Objectives]This study was conducted to investigate the molecular characteristics,genetic structure and evolutionary mechanism of cucurbit aphid-borne yellows virus(CABYV),which is of great significance for clarifying...[Objectives]This study was conducted to investigate the molecular characteristics,genetic structure and evolutionary mechanism of cucurbit aphid-borne yellows virus(CABYV),which is of great significance for clarifying the epidemic laws of the disease in the field and formulating long-term sustainable control strategies.[Methods]120 melon leaves suspected to be infected with CABYV were randomly collected from Aksu City,Xinjiang.RT-PCR was used to detect the virus.MEGA 7.0 was used to construct a phylogenetic tree.DnaSP v5.10 was used to analyze the genetic diversity among different sequences,and RDP v.4.31 software was used to analyze possible recombination events of CABYV sequence.[Results]The detection rate of CABYV was 31.67%.A new CABYV-2 was isolated,sequenced and cloned from these positive samples.The length of the genome is 5497 bp,encoding six open reading frames.Compared with 23 CABYV strains isolated from different countries in NCBI database,CABYV-2 shared the highest homology with KR231942.1 and the lowest homology with JF939812.1.The results of evolutionary tree analysis showed that the 24 isolates were divided into two branches due to different geographical factors,including CABYV-2 and KR2319421,which were clustered together and belonged to branch 1.The results of genetic diversity and neutrality tests showed that CABYV was highly variable and the population was expanding.The results of recombination analysis showed that there were two recombinants,EU636992.1 and KR231949.1,which exacerbated the variation of CABYV.[Conclusions]The CABYV isolate from Aksu City,Xinjiang had the closest relationship with the Korean isolate KR231942.1,and the farthest relationship with the isolates from European countries.There were large genetic differences between the isolates of branch 1 and branch 2,and the recombination of CABYV in different hosts aggravated the variation of CABYV.Recombination and negative selection may be important reasons for the genetic variation of CABYV.展开更多
基金This work was supported by the Innovation Group Development Project of the Ministry of Education of China(No.IRT0435).
文摘The epidermal growth factor(EGF)has been shown to promote the proliferation of various types of cells,to maintain the physiological function of the mucosa of the digestive tract,and to promote the healing of the gastric and duodenal ulcers.It has been expressed in many types of bacteria and yeasts.In this article,a bio-reactor was constructed,namely,the human EGF(hEGF)transgenic mini-tomato.On the basis of hEGF gene sequence,a tomato codon preference hEGF gene was chemically synthesized,and it was constructed into the plant expression vector pCAMBIA2300.The transgenic tomato plants containing gene hEGF were obtained through Agrobacterium-mediated transformation.The expression product was determined by radioimmunoassay(RIA)and showed a yield of 3.48±1.01 ng/g fresh fruits.The intragastric gavage(ig)administration of the rhEGF-containing juice of the transgenic tomato(equivalent to 24 ng rhEGF per mouse a day)for 15 days could significantly protect mice against the alcohol-induced ulceration.The ulcer index,expressed as a degree of the stomach lesion,decreased from 42.20±18.13 to 16.25±9.57.
文摘In this editorial,we have taken an in-depth look at the article published by Wan et al.The study showed that preconditioning mesenchymal stem cells(MSCs)protected them against programmed cell death,and increased their survival rate and therapeutic potential.Autophagy,a type of programmed cell death,is a major intracellular degradation and recycling pathway that is crucial for maintaining cellular homeostasis,self-renewal,and pluripotency.We have explored the relationship between autophagy and MSCs to determine the role of autophagy in the therapeutic applications of MSCs.
基金Supported by the National Natural Science Foundation of China(91541202,51276163)
文摘A series of 2D direct numerical simulations were performed with an accurate level set method for single drop impacts.The adopted ACLS method was validated to be efficient with perfect mass conservation in both normal and oblique impacts.A square-root correction for neck bases was modified in accuracy as well as scope of applications.In addition,process of jet formation and evolution was studied to reveal internal dynamics in drop impacts.It's found that pressure gradient and vortex are coexisting and completive reasons for jet topology while the inclined angle has a significant effect on them.Mechanisms of jet formation and evolution are different in the front and back necks.With the help of PDF distribution and correction calculation,a compromise in the competition is observed.This work lays a solid foundation for further studies of dynamics in gas-liquid flows.
基金Supported by National Natural Science Foundation of China(31760511)Linfen Key R&D Program(2009)+1 种基金2020 National Key R&D Program-Technology Helps the Economy 2020 Key Project(2020-NK-ZL34)Fundamental Research Program of Shanxi Province(201901D211403)。
文摘[Objectives]This study was conducted to investigate the molecular characteristics,genetic structure and evolutionary mechanism of cucurbit aphid-borne yellows virus(CABYV),which is of great significance for clarifying the epidemic laws of the disease in the field and formulating long-term sustainable control strategies.[Methods]120 melon leaves suspected to be infected with CABYV were randomly collected from Aksu City,Xinjiang.RT-PCR was used to detect the virus.MEGA 7.0 was used to construct a phylogenetic tree.DnaSP v5.10 was used to analyze the genetic diversity among different sequences,and RDP v.4.31 software was used to analyze possible recombination events of CABYV sequence.[Results]The detection rate of CABYV was 31.67%.A new CABYV-2 was isolated,sequenced and cloned from these positive samples.The length of the genome is 5497 bp,encoding six open reading frames.Compared with 23 CABYV strains isolated from different countries in NCBI database,CABYV-2 shared the highest homology with KR231942.1 and the lowest homology with JF939812.1.The results of evolutionary tree analysis showed that the 24 isolates were divided into two branches due to different geographical factors,including CABYV-2 and KR2319421,which were clustered together and belonged to branch 1.The results of genetic diversity and neutrality tests showed that CABYV was highly variable and the population was expanding.The results of recombination analysis showed that there were two recombinants,EU636992.1 and KR231949.1,which exacerbated the variation of CABYV.[Conclusions]The CABYV isolate from Aksu City,Xinjiang had the closest relationship with the Korean isolate KR231942.1,and the farthest relationship with the isolates from European countries.There were large genetic differences between the isolates of branch 1 and branch 2,and the recombination of CABYV in different hosts aggravated the variation of CABYV.Recombination and negative selection may be important reasons for the genetic variation of CABYV.
