Subcritical crack growth(SCG)in fluid-rock interactions plays a crucial role in understanding crustal deformation and fracture network development.Using a double-torsion technique,the subcritical crack growth and frac...Subcritical crack growth(SCG)in fluid-rock interactions plays a crucial role in understanding crustal deformation and fracture network development.Using a double-torsion technique,the subcritical crack growth and fracture characteristics of Zhangzhou granite were investigated under fluids with different pH values.Subcritical crack growth index(SCI)was reduced in both acidic and alkaline fluids compared with the neutral environment,with reduction percentages of 9.8%e31.9%under acidic environment(pH=1-5)and 8.3%e17.5%under alkaline environment(pH=10-14),respectively.In contrast,the weakening effect of fluid pH values on critical stress intensity was less than that of SCI.Scanning electron microscopy(SEM)results showed that grain boundaries were prone to dissolution compared to the basal surface,proving that subcritical cracks preferentially propagate along the grain boundaries.Fracture toughness was insensitive to fluid pH values in the short term but sensitive to solution salinity.Considering mineral compositions and contents,a rock dissolution rate was defined,and a SCI prediction model was proposed,which was demonstrated to be capable of estimating variations in SCI under various fluid environments for different rock types and could provide valuable insights for engineering applications and environmental assessments.展开更多
Background:Lactobacillus reuteri strains are widely used as probiotics to prevent and treat inflammatory bowel disease by modulating the host’s immune system.However,the underlying mechanisms by which they communicat...Background:Lactobacillus reuteri strains are widely used as probiotics to prevent and treat inflammatory bowel disease by modulating the host’s immune system.However,the underlying mechanisms by which they communicate with the host have not been clearly understood.Bacterial extracellular vesicles(EVs)have been considered as important mediators of host-pathogen interactions,but their potential role in commensals-host crosstalk has not been widely studied.Here,we investigated the regulatory actions of EVs produced by L.reuteri BBC3,a gut-associated commensal bacterium of Black-Bone chicken,in the development of lipopolysaccharide(LPS)-induced intestinal inflammation in a chicken model using both in vivo and in vitro experiments.Results:L.reuteri BBC3 produced nano-scale membrane vesicles with the size range of 60-250 nm.Biochemical and proteomic analyses showed that L.reuteri BBC3-derived EVs(LrEVs)carried DNA,RNA and several bioactive proteins previously described as mediators of other probiotics’beneficial effects such as glucosyltransferase,serine protease and elongation factor Tu.In vivo broiler experiments showed that administration of LrEVs exerted similar effects as L.reuteri BBC3 in attenuating LPS-induced inflammation by improving growth performance,reducing mortality and decreasing intestinal injury.LrEVs suppressed the LPS-induced expression of pro-inflammatory genes(TNF-α,IL-1β,IL-6,IL-17 and IL-8),and improved the expression of anti-inflammatory genes(IL-10 and TGF-β)in the jejunum.LrEVs could be internalized by chicken macrophages.In vitro pretreatment with LrEVs reduced the gene expression of TNF-α,IL-1βand IL-6 by suppressing the NF-κB activity,and enhanced the gene expression of IL-10 and TGF-βin LPS-activated chicken macrophages.Additionally,LrEVs could inhibit Th1-and Th17-mediated inflammatory responses and enhance the immunoregulatory cells-mediated immunosuppression in splenic lymphocytes of LPS-challenged chickens through the activation of macrophages.Finally,we revealed that the reduced content of both vesicular proteins and nucleic acids attenuated the suppression of LrEVs on LPS-induced inflammatory responses in ex vivo experiments,suggesting that they are essential for the LrEVs-mediated immunoregulation.Conclusions:We revealed that LrEVs participated in maintaining intestinal immune homeostasis against LPSinduced inflammatory responses in a chicken model.Our findings provide mechanistic insight into how commensal and probiotic Lactobacillus species modulate the host’s immune system in pathogens-induced inflammation.展开更多
Consistency degree calculation is established on the basis of known correspondence, but in real life, the correspondence is generally unknown, so how to calculate consistency of two models under unknown correspondence...Consistency degree calculation is established on the basis of known correspondence, but in real life, the correspondence is generally unknown, so how to calculate consistency of two models under unknown correspondence has become a problem. For this condition, we should analyze unknown correspondence due to the influence of different correspondences.In this paper we obtain the relations of transitions based on event relations using branching processes, and build a behavioral matrix of relations. Based on the permutation of behavioral matrix, we express different correspondences, and define a new formula to compute the maximal consistency degree of two workflow nets. Additionally, this paper utilizes an example to show these definitions, computation as well as the advantages.展开更多
This study introduces a coupled electromagnetic–thermal–mechanical model to reveal the mechanisms of microcracking and mineral melting of polymineralic rocks under microwave radiation.Experimental tests validate the...This study introduces a coupled electromagnetic–thermal–mechanical model to reveal the mechanisms of microcracking and mineral melting of polymineralic rocks under microwave radiation.Experimental tests validate the rationality of the proposed model.Embedding microscopic mineral sections into the granite model for simulation shows that uneven temperature gradients create distinct molten,porous,and nonmolten zones on the fracture surface.Moreover,the varying thermal expansion coefficients and Young's moduli among the minerals induce significant thermal stress at the mineral boundaries.Quartz and biotite with higher thermal expansion coefficients are subjected to compression,whereas plagioclase with smaller coefficients experiences tensile stress.In the molten zone,quartz undergoes transgranular cracking due to theα–βphase transition.The local high temperatures also induce melting phase transitions in biotite and feldspar.This numerical study provides new insights into the distribution of thermal stress and mineral phase changes in rocks under microwave irradiation.展开更多
Currently,the video captioning models based on an encoder-decoder mainly rely on a single video input source.The contents of video captioning are limited since few studies employed external corpus information to guide...Currently,the video captioning models based on an encoder-decoder mainly rely on a single video input source.The contents of video captioning are limited since few studies employed external corpus information to guide the generation of video captioning,which is not conducive to the accurate descrip-tion and understanding of video content.To address this issue,a novel video captioning method guided by a sentence retrieval generation network(ED-SRG)is proposed in this paper.First,a ResNeXt network model,an efficient convolutional network for online video understanding(ECO)model,and a long short-term memory(LSTM)network model are integrated to construct an encoder-decoder,which is utilized to extract the 2D features,3D features,and object features of video data respectively.These features are decoded to generate textual sentences that conform to video content for sentence retrieval.Then,a sentence-transformer network model is employed to retrieve different sentences in an external corpus that are semantically similar to the above textual sentences.The candidate sentences are screened out through similarity measurement.Finally,a novel GPT-2 network model is constructed based on GPT-2 network structure.The model introduces a designed random selector to randomly select predicted words with a high probability in the corpus,which is used to guide and generate textual sentences that are more in line with human natural language expressions.The proposed method in this paper is compared with several existing works by experiments.The results show that the indicators BLEU-4,CIDEr,ROUGE_L,and METEOR are improved by 3.1%,1.3%,0.3%,and 1.5%on a public dataset MSVD and 1.3%,0.5%,0.2%,1.9%on a public dataset MSR-VTT respectively.It can be seen that the proposed method in this paper can generate video captioning with richer semantics than several state-of-the-art approaches.展开更多
The passwords for unlocking the mobile devices are relatively simple,easier to be stolen,which causes serious potential security problems.An important research direction of identity authentication is to establish user...The passwords for unlocking the mobile devices are relatively simple,easier to be stolen,which causes serious potential security problems.An important research direction of identity authentication is to establish user behavior models to authenticate users.In this paper,a mobile terminal APP browsing behavioral authentication system architecture which synthesizes multiple factors is designed.This architecture is suitable for users using the mobile terminal APP in the daily life.The architecture includes data acquisition,data processing,feature extraction,and sub model training.We can use this architecture for continuous authentication when the user uses APP at the mobile terminal.展开更多
Background:Despite integrin being highlighted as a stiffness-sensor molecule in matrix stiffness-driven angiogenesis,other stiffness-sensor molecules and their mechanosensory pathways related to angiogenesis in hepato...Background:Despite integrin being highlighted as a stiffness-sensor molecule in matrix stiffness-driven angiogenesis,other stiffness-sensor molecules and their mechanosensory pathways related to angiogenesis in hepatocellular carcinoma(HCC)remain obscure.Here,we explored the interplay between Piezo1 and integrinβ1 in the mechanosensory pathway and their effects on HCC angiogenesis to better understand matrix stiffness-induced angiogenesis.Methods:The role of Piezo1 in matrix stiffness-induced angiogenesis was investigated using orthotopic liver cancer SD rat models with high liver stiffness background,and its clinical significance was evaluated in human HCC tissues.Matrix stiffness-mediated Piezo1 upregulation and activation were assayed using an in vitro fibronectin(FN)-coated cell culture system with different stiffness,Western blotting and Ca^(2+)probe.The effects of shPiezo1-conditioned medium(CM)on angiogenesis were examined by tube formation assay,wound healing assay and angiogenesis array.The underlying mechanism by which Piezo1 participated in matrix stiffness-induced angiogenesis was analyzed by microRNA quantitative real-time polymerase chain reaction(qRT-PCR),matrix stiffness measurement,dual-luciferase reporter assay,ubiquitination assay and co-immunoprecipitation.Results:Increased matrix stiffness significantly upregulated Piezo1 expression at both cellular and tissue levels,and high expression of Piezo1 indicated an unfavorable prognosis.High matrix stiffness also noticeably enhanced the activation level of Piezo1,similar to its expression level.Piezo1 knockdown significantly suppressed tumor growth,angiogenesis,and lung metastasis of HCC rat models with high liver stiffness background.shPiezo1-CM from HCC cells attenuated tube formation and migration abilities of vascular endothelial cells remarkably,and analysis of differentially expressed pro-angiogenic factors revealed that Piezo1 promoted the expression and secretion of vascular endothelial growth factor(VEGF),CXC chemokine ligand 16(CXCL16)and insulin-like growth factor binding protein 2(IGFBP2).Matrix stiffness-caused Piezo1 upregulation/activation restrained hypoxia inducible factor-1α(HIF-1α)ubiquitination,subsequently enhanced the expression of downstream pro-angiogenic factors to accelerate HCC angiogenesis.Besides,collagen 1(COL1)-reinforced tissue stiffening resulted in more expression of Piezo1 via miR-625-5p.Conclusions:This study unravels a new mechanism by which the inte-grinβ1/Piezo1 activation/Ca2+influx/HIF-1αubiquitination/VEGF,CXCL16 and IGFBP2 pathway participates in matrix stiffness-driven HCC angiogenesis.Simultaneously,a positive feedback regulation loop as stiff matrix/integrinβ1/miR-625-5p/Piezo1 and COL1/stiffer matrix mediates matrix stiffness-caused Piezo1 upregulation.展开更多
基金financial support from the National Natural Science Foundation of China(No.52074349)China Scholarship Council(No.202206370108)the Hunan Provincial Innovation Foundation for Postgraduate(No.CX20230193).
文摘Subcritical crack growth(SCG)in fluid-rock interactions plays a crucial role in understanding crustal deformation and fracture network development.Using a double-torsion technique,the subcritical crack growth and fracture characteristics of Zhangzhou granite were investigated under fluids with different pH values.Subcritical crack growth index(SCI)was reduced in both acidic and alkaline fluids compared with the neutral environment,with reduction percentages of 9.8%e31.9%under acidic environment(pH=1-5)and 8.3%e17.5%under alkaline environment(pH=10-14),respectively.In contrast,the weakening effect of fluid pH values on critical stress intensity was less than that of SCI.Scanning electron microscopy(SEM)results showed that grain boundaries were prone to dissolution compared to the basal surface,proving that subcritical cracks preferentially propagate along the grain boundaries.Fracture toughness was insensitive to fluid pH values in the short term but sensitive to solution salinity.Considering mineral compositions and contents,a rock dissolution rate was defined,and a SCI prediction model was proposed,which was demonstrated to be capable of estimating variations in SCI under various fluid environments for different rock types and could provide valuable insights for engineering applications and environmental assessments.
基金This study was supported by the National Natural Science Foundation of China(No.31372343 and 31672437).
文摘Background:Lactobacillus reuteri strains are widely used as probiotics to prevent and treat inflammatory bowel disease by modulating the host’s immune system.However,the underlying mechanisms by which they communicate with the host have not been clearly understood.Bacterial extracellular vesicles(EVs)have been considered as important mediators of host-pathogen interactions,but their potential role in commensals-host crosstalk has not been widely studied.Here,we investigated the regulatory actions of EVs produced by L.reuteri BBC3,a gut-associated commensal bacterium of Black-Bone chicken,in the development of lipopolysaccharide(LPS)-induced intestinal inflammation in a chicken model using both in vivo and in vitro experiments.Results:L.reuteri BBC3 produced nano-scale membrane vesicles with the size range of 60-250 nm.Biochemical and proteomic analyses showed that L.reuteri BBC3-derived EVs(LrEVs)carried DNA,RNA and several bioactive proteins previously described as mediators of other probiotics’beneficial effects such as glucosyltransferase,serine protease and elongation factor Tu.In vivo broiler experiments showed that administration of LrEVs exerted similar effects as L.reuteri BBC3 in attenuating LPS-induced inflammation by improving growth performance,reducing mortality and decreasing intestinal injury.LrEVs suppressed the LPS-induced expression of pro-inflammatory genes(TNF-α,IL-1β,IL-6,IL-17 and IL-8),and improved the expression of anti-inflammatory genes(IL-10 and TGF-β)in the jejunum.LrEVs could be internalized by chicken macrophages.In vitro pretreatment with LrEVs reduced the gene expression of TNF-α,IL-1βand IL-6 by suppressing the NF-κB activity,and enhanced the gene expression of IL-10 and TGF-βin LPS-activated chicken macrophages.Additionally,LrEVs could inhibit Th1-and Th17-mediated inflammatory responses and enhance the immunoregulatory cells-mediated immunosuppression in splenic lymphocytes of LPS-challenged chickens through the activation of macrophages.Finally,we revealed that the reduced content of both vesicular proteins and nucleic acids attenuated the suppression of LrEVs on LPS-induced inflammatory responses in ex vivo experiments,suggesting that they are essential for the LrEVs-mediated immunoregulation.Conclusions:We revealed that LrEVs participated in maintaining intestinal immune homeostasis against LPSinduced inflammatory responses in a chicken model.Our findings provide mechanistic insight into how commensal and probiotic Lactobacillus species modulate the host’s immune system in pathogens-induced inflammation.
基金supported in part by the National Key R&D Program of China(2017YFB1001804)Shanghai Science and Technology Innovation Action Plan Project(16511100900)the National Natural Science Foundation of China(61572360)
文摘Consistency degree calculation is established on the basis of known correspondence, but in real life, the correspondence is generally unknown, so how to calculate consistency of two models under unknown correspondence has become a problem. For this condition, we should analyze unknown correspondence due to the influence of different correspondences.In this paper we obtain the relations of transitions based on event relations using branching processes, and build a behavioral matrix of relations. Based on the permutation of behavioral matrix, we express different correspondences, and define a new formula to compute the maximal consistency degree of two workflow nets. Additionally, this paper utilizes an example to show these definitions, computation as well as the advantages.
基金the National Natural Science Foundation of China(No.52074349)the Graduate Research Innovation Project of Hunan Province,China(No.CX20230194)。
文摘This study introduces a coupled electromagnetic–thermal–mechanical model to reveal the mechanisms of microcracking and mineral melting of polymineralic rocks under microwave radiation.Experimental tests validate the rationality of the proposed model.Embedding microscopic mineral sections into the granite model for simulation shows that uneven temperature gradients create distinct molten,porous,and nonmolten zones on the fracture surface.Moreover,the varying thermal expansion coefficients and Young's moduli among the minerals induce significant thermal stress at the mineral boundaries.Quartz and biotite with higher thermal expansion coefficients are subjected to compression,whereas plagioclase with smaller coefficients experiences tensile stress.In the molten zone,quartz undergoes transgranular cracking due to theα–βphase transition.The local high temperatures also induce melting phase transitions in biotite and feldspar.This numerical study provides new insights into the distribution of thermal stress and mineral phase changes in rocks under microwave irradiation.
基金supported in part by the National Natural Science Foundation of China under Grants 62273272 and 61873277in part by the Chinese Postdoctoral Science Foundation under Grant 2020M673446+1 种基金in part by the Key Research and Development Program of Shaanxi Province under Grant 2023-YBGY-243in part by the Youth Innovation Team of Shaanxi Universities.
文摘Currently,the video captioning models based on an encoder-decoder mainly rely on a single video input source.The contents of video captioning are limited since few studies employed external corpus information to guide the generation of video captioning,which is not conducive to the accurate descrip-tion and understanding of video content.To address this issue,a novel video captioning method guided by a sentence retrieval generation network(ED-SRG)is proposed in this paper.First,a ResNeXt network model,an efficient convolutional network for online video understanding(ECO)model,and a long short-term memory(LSTM)network model are integrated to construct an encoder-decoder,which is utilized to extract the 2D features,3D features,and object features of video data respectively.These features are decoded to generate textual sentences that conform to video content for sentence retrieval.Then,a sentence-transformer network model is employed to retrieve different sentences in an external corpus that are semantically similar to the above textual sentences.The candidate sentences are screened out through similarity measurement.Finally,a novel GPT-2 network model is constructed based on GPT-2 network structure.The model introduces a designed random selector to randomly select predicted words with a high probability in the corpus,which is used to guide and generate textual sentences that are more in line with human natural language expressions.The proposed method in this paper is compared with several existing works by experiments.The results show that the indicators BLEU-4,CIDEr,ROUGE_L,and METEOR are improved by 3.1%,1.3%,0.3%,and 1.5%on a public dataset MSVD and 1.3%,0.5%,0.2%,1.9%on a public dataset MSR-VTT respectively.It can be seen that the proposed method in this paper can generate video captioning with richer semantics than several state-of-the-art approaches.
基金partially supported by the National Key Research and Development Program of China(2018YFB2100801)。
文摘The passwords for unlocking the mobile devices are relatively simple,easier to be stolen,which causes serious potential security problems.An important research direction of identity authentication is to establish user behavior models to authenticate users.In this paper,a mobile terminal APP browsing behavioral authentication system architecture which synthesizes multiple factors is designed.This architecture is suitable for users using the mobile terminal APP in the daily life.The architecture includes data acquisition,data processing,feature extraction,and sub model training.We can use this architecture for continuous authentication when the user uses APP at the mobile terminal.
基金ational Natural Science Foundation of China,Grant/Award Number:81972910。
文摘Background:Despite integrin being highlighted as a stiffness-sensor molecule in matrix stiffness-driven angiogenesis,other stiffness-sensor molecules and their mechanosensory pathways related to angiogenesis in hepatocellular carcinoma(HCC)remain obscure.Here,we explored the interplay between Piezo1 and integrinβ1 in the mechanosensory pathway and their effects on HCC angiogenesis to better understand matrix stiffness-induced angiogenesis.Methods:The role of Piezo1 in matrix stiffness-induced angiogenesis was investigated using orthotopic liver cancer SD rat models with high liver stiffness background,and its clinical significance was evaluated in human HCC tissues.Matrix stiffness-mediated Piezo1 upregulation and activation were assayed using an in vitro fibronectin(FN)-coated cell culture system with different stiffness,Western blotting and Ca^(2+)probe.The effects of shPiezo1-conditioned medium(CM)on angiogenesis were examined by tube formation assay,wound healing assay and angiogenesis array.The underlying mechanism by which Piezo1 participated in matrix stiffness-induced angiogenesis was analyzed by microRNA quantitative real-time polymerase chain reaction(qRT-PCR),matrix stiffness measurement,dual-luciferase reporter assay,ubiquitination assay and co-immunoprecipitation.Results:Increased matrix stiffness significantly upregulated Piezo1 expression at both cellular and tissue levels,and high expression of Piezo1 indicated an unfavorable prognosis.High matrix stiffness also noticeably enhanced the activation level of Piezo1,similar to its expression level.Piezo1 knockdown significantly suppressed tumor growth,angiogenesis,and lung metastasis of HCC rat models with high liver stiffness background.shPiezo1-CM from HCC cells attenuated tube formation and migration abilities of vascular endothelial cells remarkably,and analysis of differentially expressed pro-angiogenic factors revealed that Piezo1 promoted the expression and secretion of vascular endothelial growth factor(VEGF),CXC chemokine ligand 16(CXCL16)and insulin-like growth factor binding protein 2(IGFBP2).Matrix stiffness-caused Piezo1 upregulation/activation restrained hypoxia inducible factor-1α(HIF-1α)ubiquitination,subsequently enhanced the expression of downstream pro-angiogenic factors to accelerate HCC angiogenesis.Besides,collagen 1(COL1)-reinforced tissue stiffening resulted in more expression of Piezo1 via miR-625-5p.Conclusions:This study unravels a new mechanism by which the inte-grinβ1/Piezo1 activation/Ca2+influx/HIF-1αubiquitination/VEGF,CXCL16 and IGFBP2 pathway participates in matrix stiffness-driven HCC angiogenesis.Simultaneously,a positive feedback regulation loop as stiff matrix/integrinβ1/miR-625-5p/Piezo1 and COL1/stiffer matrix mediates matrix stiffness-caused Piezo1 upregulation.
