Owing to its unique ability to capture volumetric tomographic information with a single light flash,optoacoustic(OA)tomography has recently demonstrated ultrafast imaging speeds ultimately limited by the ultrasound ti...Owing to its unique ability to capture volumetric tomographic information with a single light flash,optoacoustic(OA)tomography has recently demonstrated ultrafast imaging speeds ultimately limited by the ultrasound time-of-flight.The method's scalability and the achievable spatial resolution are yet limited by the narrow bandwidth of piezo-composite arrays currently employed for OA signal detection.Here we report on the first implementation of high-density spherical array technology based on flexible polyvinylidene difluoride films featuring ultrawideband(0.3-40 MHz)sub mm^(2)area elements,thus enabling real-time multi-scale volumetric imaging with 22-35μm spatial resolution,superior image fidelity and over an order of magnitude signal-to-noise enhancement compared to piezo-composite equivalents.We further demonstrate five-dimensional(spectroscopic,time-resolved,volumetric)imaging capabilities by visualizing fast stimulus-evoked cerebral oxygenation changes in mice and performing real-time functional angiography of deep human micro-vasculature.The new technology thus leverages the true potential of OA for quantitative high-resolution visualization of rapid bio-dynamics across scales.展开更多
基金supported by the Swiss National Science Foundation(310030_192757)the European Research Council(ERC-2015-CoG-682379)+2 种基金The development of the ultra-wideband ultrasonic antenna and the in vivo optoacoustic experiments were supported by the grants from the Russian Science Foundation(18-45-06006)the Helmholtz Association(HRSF-0020)The development of numerical algorithms for enhancement of angiographic optoacoustic images was partially supported by the grant from the Russian Science Foundation(19-75-10055)。
文摘Owing to its unique ability to capture volumetric tomographic information with a single light flash,optoacoustic(OA)tomography has recently demonstrated ultrafast imaging speeds ultimately limited by the ultrasound time-of-flight.The method's scalability and the achievable spatial resolution are yet limited by the narrow bandwidth of piezo-composite arrays currently employed for OA signal detection.Here we report on the first implementation of high-density spherical array technology based on flexible polyvinylidene difluoride films featuring ultrawideband(0.3-40 MHz)sub mm^(2)area elements,thus enabling real-time multi-scale volumetric imaging with 22-35μm spatial resolution,superior image fidelity and over an order of magnitude signal-to-noise enhancement compared to piezo-composite equivalents.We further demonstrate five-dimensional(spectroscopic,time-resolved,volumetric)imaging capabilities by visualizing fast stimulus-evoked cerebral oxygenation changes in mice and performing real-time functional angiography of deep human micro-vasculature.The new technology thus leverages the true potential of OA for quantitative high-resolution visualization of rapid bio-dynamics across scales.
