Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery,ischemic states and multiple organ failure.The pre-existing nutritional status of the liver,i.e.,fasting,might contribute to the ex...Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery,ischemic states and multiple organ failure.The pre-existing nutritional status of the liver,i.e.,fasting,might contribute to the extent of tissue injury.This study investigated whether alanine,an amino acid precursor of glucose,could protect ex vivo perfused livers of fasting rats from reperfusion injury.The portal vein was cannulated,the liver re-moved and perfused in a closed ex vivo system.Isolated livers were perfused either with glucose 1 g/L and 10 g/L,or with equal concentrations of alanine(n=10 in each group).The experiment consisted of perfusion for 15 min,ischemia for 60 min,and reoxygenation during 60 min.Enzymes,glucose,lactate and bilirubin were analysed in perfusate samples.The proportion of glycogen as well as activation of caspase 3 was determined in biopsies.Alanine at a concentration of 10 g/L attenuated enzymes release in the perfusate during reoxygenation when com-pared to glucose-treated groups.Lactate level in the perfusate was lowest in alanine groups.Ischemia-reperfusion and mainly alanine activated apoptosis,specifically in Kupffer and endothelial cells.Alanine presents a pro-tective effect on normothermic ischemia-reperfusion injury of the fasting rat liver when compared to glucose.展开更多
基金The authors are grateful to Laurence Seidel,MSc(Biostatistician,Department of Biostatistics,University of Liege,Liege,Belgium),to Professor Adelin Albert,PhD(Professor of Biostatistics,Department of Biostatistics,University of Liege,Liege,Belgium)for his advice and con-structive criticism so freely given and to Saad Guendouz BSc(Laboratory of Pathological Anatomy(ULB unit 724)Free University of Brussels,Brussels,Belgium)for tech-nical help.
文摘Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery,ischemic states and multiple organ failure.The pre-existing nutritional status of the liver,i.e.,fasting,might contribute to the extent of tissue injury.This study investigated whether alanine,an amino acid precursor of glucose,could protect ex vivo perfused livers of fasting rats from reperfusion injury.The portal vein was cannulated,the liver re-moved and perfused in a closed ex vivo system.Isolated livers were perfused either with glucose 1 g/L and 10 g/L,or with equal concentrations of alanine(n=10 in each group).The experiment consisted of perfusion for 15 min,ischemia for 60 min,and reoxygenation during 60 min.Enzymes,glucose,lactate and bilirubin were analysed in perfusate samples.The proportion of glycogen as well as activation of caspase 3 was determined in biopsies.Alanine at a concentration of 10 g/L attenuated enzymes release in the perfusate during reoxygenation when com-pared to glucose-treated groups.Lactate level in the perfusate was lowest in alanine groups.Ischemia-reperfusion and mainly alanine activated apoptosis,specifically in Kupffer and endothelial cells.Alanine presents a pro-tective effect on normothermic ischemia-reperfusion injury of the fasting rat liver when compared to glucose.
