Background:Aortic dissection(AD)is a fatal cardiovascular disease for which the key involved genes are largely unknown.Here,we aimed to identify promising AD biomarkers from high-throughput RNA expressing data.Methods...Background:Aortic dissection(AD)is a fatal cardiovascular disease for which the key involved genes are largely unknown.Here,we aimed to identify promising AD biomarkers from high-throughput RNA expressing data.Methods:In the GSE98770 dataset,differentially expressed mRNAs(DE-mRNAs)and microRNAs(DE-microRNAs)were identified through differentially expressed gene analysis and gene set enrichment analysis.The regulatory network between DE-mRNAs and DE-microRNAs was established,and hub genes were identified with Cytoscape.Relationships between hub genes and AD were confirmed in the Comparative Toxicogenomics Database(CTD).Potential key transcription factors were discovered with Cytoscape.Hub gene verification was performed by qPCR and immunofluorescence analyses of human specimens.Results:DE-mRNAs and DE-microRNAs were identified.Four mRNAs and microRNA-1321(miR-1321)were found to have the most connections with other genes.CBL was connected to the most genes and interacted with miR-1321,which was also connected to the most genes among the DE-microRNAs.In addition,CBL was associated with AD in the CTD.Among the top five transcription factors potentially regulating CBL transcription,only HOXB13 was a DE-mRNA.The findings were further successfully verified in human specimens.Conclusion:CBL,which may be transcriptionally regulated by HOXB13 and post-transcriptionally regulated by miR-1321,was identified as the most promising potential biomarker for AD.展开更多
Understanding the mechanical response of Q2 loess subjected to dry-wet cycles(DWCs)is the premise for the rational design of a hydraulic tunnel.Taking the Hanjiang-to-Weihe south line project in China as the research ...Understanding the mechanical response of Q2 loess subjected to dry-wet cycles(DWCs)is the premise for the rational design of a hydraulic tunnel.Taking the Hanjiang-to-Weihe south line project in China as the research background,the microstructure evolution,strength degradation and compression characteristics of Q2 loess under different DWCs were investigated,and the fluid-solid coupling analysis of the hydraulic tunnel was carried out using the FLAC3D software.The amplification effect of tunnel surrounding soil pressure(SSP)and its influence on the long-term stability of the tunnel under different DWCs were obtained.The results showed that the pore microstructure parameters of the undisturbed and remolded loess basically tend to be stable after the number of DWCs exceeds 3.The porosity of Q2 loess is increased by 26%.The internal friction angle and cohesion of Q2 loess are decreased by 35%and 31%,respectively.The vertical strain of Q2 loess is increased by 55%after considering the DWCs.After the DWCs stabilized,the SSP ratio is increased between 10%and 25%.With the increase in buried depth of the tunnel,the SSP ratio is increased by 8%e10%.The SSP is reduced from 8%to 16%by the rise in groundwater level.As the number of DWCs increases and the burial depth of the tunnel decreases,the distribution of SSP becomes progressively more non-uniform.Based on the amplification factor and the modified compressive arch theory,the SSP distribution model of loess tunnel was proposed,which can be preliminarily applied to the design of supporting structures considering DWCs.展开更多
基金supported by grants from the Guangzhou Basic and Applied Basic Research Foundation(202201010940)National Natural Science Foundation of China(82200306)Guangdong Basic and Applied Basic Research Foundation(2021A1515111092).
文摘Background:Aortic dissection(AD)is a fatal cardiovascular disease for which the key involved genes are largely unknown.Here,we aimed to identify promising AD biomarkers from high-throughput RNA expressing data.Methods:In the GSE98770 dataset,differentially expressed mRNAs(DE-mRNAs)and microRNAs(DE-microRNAs)were identified through differentially expressed gene analysis and gene set enrichment analysis.The regulatory network between DE-mRNAs and DE-microRNAs was established,and hub genes were identified with Cytoscape.Relationships between hub genes and AD were confirmed in the Comparative Toxicogenomics Database(CTD).Potential key transcription factors were discovered with Cytoscape.Hub gene verification was performed by qPCR and immunofluorescence analyses of human specimens.Results:DE-mRNAs and DE-microRNAs were identified.Four mRNAs and microRNA-1321(miR-1321)were found to have the most connections with other genes.CBL was connected to the most genes and interacted with miR-1321,which was also connected to the most genes among the DE-microRNAs.In addition,CBL was associated with AD in the CTD.Among the top five transcription factors potentially regulating CBL transcription,only HOXB13 was a DE-mRNA.The findings were further successfully verified in human specimens.Conclusion:CBL,which may be transcriptionally regulated by HOXB13 and post-transcriptionally regulated by miR-1321,was identified as the most promising potential biomarker for AD.
基金the National Natural Science Foundation of China(Grant Nos.52279110,52178355 and 52108339).
文摘Understanding the mechanical response of Q2 loess subjected to dry-wet cycles(DWCs)is the premise for the rational design of a hydraulic tunnel.Taking the Hanjiang-to-Weihe south line project in China as the research background,the microstructure evolution,strength degradation and compression characteristics of Q2 loess under different DWCs were investigated,and the fluid-solid coupling analysis of the hydraulic tunnel was carried out using the FLAC3D software.The amplification effect of tunnel surrounding soil pressure(SSP)and its influence on the long-term stability of the tunnel under different DWCs were obtained.The results showed that the pore microstructure parameters of the undisturbed and remolded loess basically tend to be stable after the number of DWCs exceeds 3.The porosity of Q2 loess is increased by 26%.The internal friction angle and cohesion of Q2 loess are decreased by 35%and 31%,respectively.The vertical strain of Q2 loess is increased by 55%after considering the DWCs.After the DWCs stabilized,the SSP ratio is increased between 10%and 25%.With the increase in buried depth of the tunnel,the SSP ratio is increased by 8%e10%.The SSP is reduced from 8%to 16%by the rise in groundwater level.As the number of DWCs increases and the burial depth of the tunnel decreases,the distribution of SSP becomes progressively more non-uniform.Based on the amplification factor and the modified compressive arch theory,the SSP distribution model of loess tunnel was proposed,which can be preliminarily applied to the design of supporting structures considering DWCs.
