Dear Editor,Recently,a number of new Omicron subvariants related to BA.4/5 and BA.2.75 have emerged and shown remarkable antibody evasion capacities,in particular BF.7,BQ.1,BQ.1.1,BA.2.75.2,XBB and XBB.1.5.1 Unsurpris...Dear Editor,Recently,a number of new Omicron subvariants related to BA.4/5 and BA.2.75 have emerged and shown remarkable antibody evasion capacities,in particular BF.7,BQ.1,BQ.1.1,BA.2.75.2,XBB and XBB.1.5.1 Unsurprisingly,these new subvariants are quickly gaining prevalence worldwide.In fact,some of them have outcompeted BA.5 in the USA according to CDC’s national genomic surveillance data in which,as of 6th February 2023,XBB.1.5,BQ.1.1,BQ.1,XBB and BF.7 have achieved a dominance of 66.4%,19.9%,7.3%,2.3%and 0.5%in the USA,as compared to 0.5%for BA.5.In this report,using plasma samples collected from individuals following different vaccination strategies and COVID-19 convalescent donors,we performed pseudoviral neutralization assays to confirm severe reductions in neutralization titers against BF.7,BQ.1,BQ.1.1,BA.2.75.2,XBB and XBB.1.5 in comparison to other Omicron sub-lineages.XBB and XBB.1.5 were shown to be remarkably resistant to plasma neutralization in all tested cohorts.By comparing the differential neutralization profiles,we found that a heterologous booster with an aerosolized vaccine following 2 doses of inactivated vaccine seemed to be superior to other vaccination strategies.展开更多
Dear Editor,Emerging epidemiology data indicate that the Omicron BA.2 sublineage is expected to become the dominant strain owing to its enhanced transmissibility.1 The BA.1 sublineage reduced the efficacy of neutraliz...Dear Editor,Emerging epidemiology data indicate that the Omicron BA.2 sublineage is expected to become the dominant strain owing to its enhanced transmissibility.1 The BA.1 sublineage reduced the efficacy of neutralizing antibodies.2,3 The BA.2 sublineage may possess similar neutralizing antibody evasion.3 However,booster(3rd dose)vaccination strategies,or three exposures to the SARS-CoV2 spike protein through natural infection elicits strong neutralizing antibody responses.This raises the question of whether booster doses also elicit neutralizing antibody responses against BA.2.We present in this study the neutralization activity of serum from human recipients of homologous booster(three doses of CoronaVac inactivated vaccine)or heterologous booster(inactivated vaccine priming dose with BnT162b2 third dose)vaccine regimens against pseudovirus containing the Omicron BA.2 spike protein.We also evaluated the neutralizing activities of 6 monoclonal antibodies,and serum from monkeys vaccinated with a recombinant RBD protein vaccine.Finally,we also evaluated cell entry mediated by the Omicron BA.2 spike protein.展开更多
基金Key R&D Program of China(2021YFC2300101 YW)the National Natural Science Foundation of China(82172240 YW,82025001 JZ)+4 种基金Self-supporting Program of Guangzhou Laboratory(SRPG22-001,SRPG22-006)Guangdong Basic and Applied Research Projects(2023B1515020040 YW,2020A0505100063 JZ,2019B1515120068 JZ)ZhongNanShan Medical Foundation of Guangdong Province(ZNSA-2020001,ZNSA-2020013)State Key Laboratory of Respiratory Disease(SKLRD-Z-202214 YW,SKLRD-OP-202309 YW)Guangzhou Medical University(YP2022005 YW).
文摘Dear Editor,Recently,a number of new Omicron subvariants related to BA.4/5 and BA.2.75 have emerged and shown remarkable antibody evasion capacities,in particular BF.7,BQ.1,BQ.1.1,BA.2.75.2,XBB and XBB.1.5.1 Unsurprisingly,these new subvariants are quickly gaining prevalence worldwide.In fact,some of them have outcompeted BA.5 in the USA according to CDC’s national genomic surveillance data in which,as of 6th February 2023,XBB.1.5,BQ.1.1,BQ.1,XBB and BF.7 have achieved a dominance of 66.4%,19.9%,7.3%,2.3%and 0.5%in the USA,as compared to 0.5%for BA.5.In this report,using plasma samples collected from individuals following different vaccination strategies and COVID-19 convalescent donors,we performed pseudoviral neutralization assays to confirm severe reductions in neutralization titers against BF.7,BQ.1,BQ.1.1,BA.2.75.2,XBB and XBB.1.5 in comparison to other Omicron sub-lineages.XBB and XBB.1.5 were shown to be remarkably resistant to plasma neutralization in all tested cohorts.By comparing the differential neutralization profiles,we found that a heterologous booster with an aerosolized vaccine following 2 doses of inactivated vaccine seemed to be superior to other vaccination strategies.
文摘Dear Editor,Emerging epidemiology data indicate that the Omicron BA.2 sublineage is expected to become the dominant strain owing to its enhanced transmissibility.1 The BA.1 sublineage reduced the efficacy of neutralizing antibodies.2,3 The BA.2 sublineage may possess similar neutralizing antibody evasion.3 However,booster(3rd dose)vaccination strategies,or three exposures to the SARS-CoV2 spike protein through natural infection elicits strong neutralizing antibody responses.This raises the question of whether booster doses also elicit neutralizing antibody responses against BA.2.We present in this study the neutralization activity of serum from human recipients of homologous booster(three doses of CoronaVac inactivated vaccine)or heterologous booster(inactivated vaccine priming dose with BnT162b2 third dose)vaccine regimens against pseudovirus containing the Omicron BA.2 spike protein.We also evaluated the neutralizing activities of 6 monoclonal antibodies,and serum from monkeys vaccinated with a recombinant RBD protein vaccine.Finally,we also evaluated cell entry mediated by the Omicron BA.2 spike protein.
