Aim: This paper aims at providing more information of people with hemophilia for more targeted treatment. Methods: 1149 people with hemophilia are surveyed in 45 units, and then established by EpiData software. The nu...Aim: This paper aims at providing more information of people with hemophilia for more targeted treatment. Methods: 1149 people with hemophilia are surveyed in 45 units, and then established by EpiData software. The number of people with hemophilia in 9 age intervals for the male and female is analyzed by Population Pyramid and Pareto Analysis. Results: It is found that the people with hemophilia are appearing in every age interval. Relative to the number of male patients, the number of female patients is extremely rare. Of 1134 male patients, relative frequencies for male ranked the first (23.0%), second (17.4%), third (17.2%) are 7 - 12, 2 - 3, 13 - 18 years old, respectively, while of 15 female patients, relative frequencies for female in these age intervals are only 13.3%, 13.3%, 6.7%, respectively. However, the most relative frequency of female patients is between 26 - 45 years old. Conclusion: There is a challenge of the aging hemophilia population. Most of male patients are between 7 - 12 and 2 - 3 years old. Most of female patients are between 26 - 45 years old.展开更多
Objective To supply a scientific basis for standardizing the normal reference value of forced vital capacity(FVC)of Chinese younger women.Methods We studied the relationship between the normal reference value of 21767...Objective To supply a scientific basis for standardizing the normal reference value of forced vital capacity(FVC)of Chinese younger women.Methods We studied the relationship between the normal reference value of 21767 samples of FVC of younger women and eight geographical factors in 157 areas in China.Results It was found that the correlation between geographical factors and the normal reference value of FVC of younger women was quite significant(F=5.884,P=0.000).By adopting the method of mathematical regression analysis,one regression equation was inferred:=3.146+0.00006919X1+0.01315X4-0.006966X6+0.09524X8±0.254.In the above equation,is the normal reference value of younger women’FVC(L);X1 is the altitude(m);X4 is the annual mean air temperature(℃);X6 is the annual mean relative humidity(%);X8 is the annual mean wind speed(m/s);0.254 is the value of the residual standard deviation.Conclusion If geographical values are obtained in a certain area,the normal reference value of FVC of younger women in this area can be obtained by using the regression equation.Furthermore,according to the geographical factors,China can be divided into eight regions:Northeast China Region,North China Region,Shanxi-Shaanxi-Inner Mongolia Region,middle and lower reaches of the Yangzte River Region,Southeast China Region,Northwest China Region,Southwest China Region,and Qinghai-Tibet Plateau Region.展开更多
Background Hand,foot,and mouth disease caused by enterovirus 71(EV71)infection is prevalent in the Asia-Pacific region in recent years.Currently,no drug is available for the prevention and treatment of EV71 infection....Background Hand,foot,and mouth disease caused by enterovirus 71(EV71)infection is prevalent in the Asia-Pacific region in recent years.Currently,no drug is available for the prevention and treatment of EV71 infection.IMB-0523,a N-phenylbenzamide derivative,inhibits hepatitis B virus replication by upregulating the expression of APOBEC3G.In the present study,the effect of IMB-0523 on EV71 replication and related mechanism were investigated.Methods The cytotoxicity of IMB-0523 was determined by cell counting kit.Quantitative real-time polymerase chain reaction and Western blot assay were used to detect the effect of IMB-0523 on EV71 replication and related mechanism.Cytopathic effect assay was used to investigate the effect of IMB-0523 on different EV71 strains,coxsackievirus A16,and coxsackieviruses of group B.Results The results showed that IMB-0523 could dose-dependently inhibit EV71 replication.Preliminary mechanism studies showed that IMB-0523 could activate STAT3 signaling to upregulate the expression of interferon-stimulated genes to play an antiviral role.In addition,IMB-0523 inhibited the replication of different EV71 strains,coxsackievirus A16,and coxsackieviruses of group B.Conclusions IMB-0523 inhibits EV71 replication by activating the STAT3 signaling pathway to upregulate interferon-stimulated gene expression.IMB-0523 has broad-spectrum antiviral potential and may be used as a lead compound for the development of broad spectrum antiviral drugs.展开更多
Coxsackievirus B type 3(CVB3)is one of the major causative pathogens associated with viral meningitis and myocarditis,which are widespread in the human population and especially prevalent in neonates and children.Thes...Coxsackievirus B type 3(CVB3)is one of the major causative pathogens associated with viral meningitis and myocarditis,which are widespread in the human population and especially prevalent in neonates and children.These infections can result in dilated cardiomyopathy(DCM)and other severe clinical complications.There are no vaccines or drugs approved for the prevention or therapy of CVB3-induced diseases.During screening for anti-CVB3 candidates in our previous studies,we found that jiadifenoic acids C exhibited strong antiviral activities against CVB3 as well as other strains of Coxsackie B viruses(CVBs).The present studies were carried out to evaluate the antiviral activities of jiadifenoic acids C.Results showed that jiadifenoic acids C could reduce CVB3 RNA and proteins synthesis in a dose-dependent manner.Jiadifenoic acids C also had a similar antiviral effect on the pleconaril-resistant variant of CVB3.We further examined the impact of jiadifenoic acids C on the synthesis of viral structural and non-structural proteins,finding that jiadifenoic acids C could reduce VP1 and 3D protein production.A time-course study with Vero cells showed that jiadifenoic acids C displayed significant antiviral activities at 0–6 h after CVB3 inoculation,indicating that jiadifenoic acids C functioned at an early step of CVB3 replication.However,jiadifenoic acids C had no prophylactic effect against CVB3.Taken together,we show that jiadifenoic acids C exhibit strong antiviral activities against all strains of CVB,including the pleconaril-resistant variant.Our study could provide a significant lead for anti-CVB3 drug development.展开更多
文摘Aim: This paper aims at providing more information of people with hemophilia for more targeted treatment. Methods: 1149 people with hemophilia are surveyed in 45 units, and then established by EpiData software. The number of people with hemophilia in 9 age intervals for the male and female is analyzed by Population Pyramid and Pareto Analysis. Results: It is found that the people with hemophilia are appearing in every age interval. Relative to the number of male patients, the number of female patients is extremely rare. Of 1134 male patients, relative frequencies for male ranked the first (23.0%), second (17.4%), third (17.2%) are 7 - 12, 2 - 3, 13 - 18 years old, respectively, while of 15 female patients, relative frequencies for female in these age intervals are only 13.3%, 13.3%, 6.7%, respectively. However, the most relative frequency of female patients is between 26 - 45 years old. Conclusion: There is a challenge of the aging hemophilia population. Most of male patients are between 7 - 12 and 2 - 3 years old. Most of female patients are between 26 - 45 years old.
基金supported by the National Natural Science Foundation of China(No.40671005)
文摘Objective To supply a scientific basis for standardizing the normal reference value of forced vital capacity(FVC)of Chinese younger women.Methods We studied the relationship between the normal reference value of 21767 samples of FVC of younger women and eight geographical factors in 157 areas in China.Results It was found that the correlation between geographical factors and the normal reference value of FVC of younger women was quite significant(F=5.884,P=0.000).By adopting the method of mathematical regression analysis,one regression equation was inferred:=3.146+0.00006919X1+0.01315X4-0.006966X6+0.09524X8±0.254.In the above equation,is the normal reference value of younger women’FVC(L);X1 is the altitude(m);X4 is the annual mean air temperature(℃);X6 is the annual mean relative humidity(%);X8 is the annual mean wind speed(m/s);0.254 is the value of the residual standard deviation.Conclusion If geographical values are obtained in a certain area,the normal reference value of FVC of younger women in this area can be obtained by using the regression equation.Furthermore,according to the geographical factors,China can be divided into eight regions:Northeast China Region,North China Region,Shanxi-Shaanxi-Inner Mongolia Region,middle and lower reaches of the Yangzte River Region,Southeast China Region,Northwest China Region,Southwest China Region,and Qinghai-Tibet Plateau Region.
基金CAMS Innovation Fund for Medical Sciences(2021-I2M-1-030)National Science and Technology Major Projects for"Major New Drugs Innovation and Development"(2018ZX09711003).
文摘Background Hand,foot,and mouth disease caused by enterovirus 71(EV71)infection is prevalent in the Asia-Pacific region in recent years.Currently,no drug is available for the prevention and treatment of EV71 infection.IMB-0523,a N-phenylbenzamide derivative,inhibits hepatitis B virus replication by upregulating the expression of APOBEC3G.In the present study,the effect of IMB-0523 on EV71 replication and related mechanism were investigated.Methods The cytotoxicity of IMB-0523 was determined by cell counting kit.Quantitative real-time polymerase chain reaction and Western blot assay were used to detect the effect of IMB-0523 on EV71 replication and related mechanism.Cytopathic effect assay was used to investigate the effect of IMB-0523 on different EV71 strains,coxsackievirus A16,and coxsackieviruses of group B.Results The results showed that IMB-0523 could dose-dependently inhibit EV71 replication.Preliminary mechanism studies showed that IMB-0523 could activate STAT3 signaling to upregulate the expression of interferon-stimulated genes to play an antiviral role.In addition,IMB-0523 inhibited the replication of different EV71 strains,coxsackievirus A16,and coxsackieviruses of group B.Conclusions IMB-0523 inhibits EV71 replication by activating the STAT3 signaling pathway to upregulate interferon-stimulated gene expression.IMB-0523 has broad-spectrum antiviral potential and may be used as a lead compound for the development of broad spectrum antiviral drugs.
基金This work was supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China(2012ZX09301002-001-015 and 2012ZX10004501-004-001).
文摘Coxsackievirus B type 3(CVB3)is one of the major causative pathogens associated with viral meningitis and myocarditis,which are widespread in the human population and especially prevalent in neonates and children.These infections can result in dilated cardiomyopathy(DCM)and other severe clinical complications.There are no vaccines or drugs approved for the prevention or therapy of CVB3-induced diseases.During screening for anti-CVB3 candidates in our previous studies,we found that jiadifenoic acids C exhibited strong antiviral activities against CVB3 as well as other strains of Coxsackie B viruses(CVBs).The present studies were carried out to evaluate the antiviral activities of jiadifenoic acids C.Results showed that jiadifenoic acids C could reduce CVB3 RNA and proteins synthesis in a dose-dependent manner.Jiadifenoic acids C also had a similar antiviral effect on the pleconaril-resistant variant of CVB3.We further examined the impact of jiadifenoic acids C on the synthesis of viral structural and non-structural proteins,finding that jiadifenoic acids C could reduce VP1 and 3D protein production.A time-course study with Vero cells showed that jiadifenoic acids C displayed significant antiviral activities at 0–6 h after CVB3 inoculation,indicating that jiadifenoic acids C functioned at an early step of CVB3 replication.However,jiadifenoic acids C had no prophylactic effect against CVB3.Taken together,we show that jiadifenoic acids C exhibit strong antiviral activities against all strains of CVB,including the pleconaril-resistant variant.Our study could provide a significant lead for anti-CVB3 drug development.
